Click here to switch to the map view.

The map label for this gene is trpG [H]

Identifier: 109946656

GI number: 109946656

Start: 20871

End: 21458

Strand: Reverse

Name: trpG [H]

Synonym: Hac_0017

Alternate gene names: 109946656

Gene position: 21458-20871 (Counterclockwise)

Preceding gene: 109946657

Following gene: 109946655

Centisome position: 1.38

GC content: 39.8

Gene sequence:

>588_bases
ATGAAAATCTTTTTTATAGATAATTTTGATTCTTTCTCTTATAACTTGGTGTATGAATTGGAGTGCTTGGGTTATGAAGT
GGCTGTTTATCAAAACGATATTGAGCCTAGCTATCTCATGGATTTAATGCTTAAAGAATCAACAACGCCCTTATTGTTTA
TTTCGCCTGGGCCTGGTAACCCTAATAGTTCGGGTAATCTTTTAGAAATCATTGCAATGGCTAAAAAGAAATTCCCCATT
TTAGGGGTTTGTTTGGGGTTGCAAGCTTTAGCGCAAAGCTATGGAGCCAAAATCATAAGGAGTAAAGAGATCGTGCATGG
CAAAGCAACGACAATCGCGCTCAAAAAGCATGCCGTTTTTAAAGGCTTAGGGGAGAGCATGGTGGTGGGGCGTTACCATT
CTTTAATGGCGAGCGGATTGCCTAAAAATTTAGAAGTGATCGCAGAACATGAAAATATCCCTATGGCTATTGTTGATGAA
AAGGATAGAATCTTAGCCTATCAATTCCACCCTGAAAGCATCATGACTTTACAAGGGAGGGCGTTACTGGAACAAAGCGT
GGGGTTTTTAAAAGGGCTATCATCATGA

Upstream 100 bases:

>100_bases
CAGGAGCTGGCATCGTTTTAGATAGCGTGCCACAAAATGAAGCGGATGAAACAAAAGCCAAAGCTAAAGCCCTTATTGAT
GCGATCAGGAAAACAAGCTT

Downstream 100 bases:

>100_bases
AAGGGATTTTAAACGCCTTGTACCATCAAAAAGACTTGAATGATGAAGAAGTCAAAAGATTATTCACTTTTATTATTAAC
GAAGAAGTAAGCCCCACGCA

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amido-transferase [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MKIFFIDNFDSFSYNLVYELECLGYEVAVYQNDIEPSYLMDLMLKESTTPLLFISPGPGNPNSSGNLLEIIAMAKKKFPI
LGVCLGLQALAQSYGAKIIRSKEIVHGKATTIALKKHAVFKGLGESMVVGRYHSLMASGLPKNLEVIAEHENIPMAIVDE
KDRILAYQFHPESIMTLQGRALLEQSVGFLKGLSS

Sequences:

>Translated_195_residues
MKIFFIDNFDSFSYNLVYELECLGYEVAVYQNDIEPSYLMDLMLKESTTPLLFISPGPGNPNSSGNLLEIIAMAKKKFPI
LGVCLGLQALAQSYGAKIIRSKEIVHGKATTIALKKHAVFKGLGESMVVGRYHSLMASGLPKNLEVIAEHENIPMAIVDE
KDRILAYQFHPESIMTLQGRALLEQSVGFLKGLSS
>Mature_195_residues
MKIFFIDNFDSFSYNLVYELECLGYEVAVYQNDIEPSYLMDLMLKESTTPLLFISPGPGNPNSSGNLLEIIAMAKKKFPI
LGVCLGLQALAQSYGAKIIRSKEIVHGKATTIALKKHAVFKGLGESMVVGRYHSLMASGLPKNLEVIAEHENIPMAIVDE
KDRILAYQFHPESIMTLQGRALLEQSVGFLKGLSS

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=189, Percent_Identity=40.7407407407407, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1789760, Length=185, Percent_Identity=40, Blast_Score=130, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322638, Length=188, Percent_Identity=37.2340425531915, Blast_Score=116, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323873, Length=109, Percent_Identity=36.697247706422, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 21543; Mature: 21543

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFFIDNFDSFSYNLVYELECLGYEVAVYQNDIEPSYLMDLMLKESTTPLLFISPGPGN
CEEEEEECCCCCEEEEEEEEEECCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCC
PNSSGNLLEIIAMAKKKFPILGVCLGLQALAQSYGAKIIRSKEIVHGKATTIALKKHAVF
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHEEECCCEECCCEEEEEEHHHHHH
KGLGESMVVGRYHSLMASGLPKNLEVIAEHENIPMAIVDEKDRILAYQFHPESIMTLQGR
HCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCEEEEEECCCCEEEECCH
ALLEQSVGFLKGLSS
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIFFIDNFDSFSYNLVYELECLGYEVAVYQNDIEPSYLMDLMLKESTTPLLFISPGPGN
CEEEEEECCCCCEEEEEEEEEECCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCC
PNSSGNLLEIIAMAKKKFPILGVCLGLQALAQSYGAKIIRSKEIVHGKATTIALKKHAVF
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHEEECCCEECCCEEEEEEHHHHHH
KGLGESMVVGRYHSLMASGLPKNLEVIAEHENIPMAIVDEKDRILAYQFHPESIMTLQGR
HCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCEEEEEECCCCEEEECCH
ALLEQSVGFLKGLSS
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]