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The map label for this gene is exoA [H]

Identifier: 109946643

GI number: 109946643

Start: 3162

End: 3914

Strand: Direct

Name: exoA [H]

Synonym: Hac_0003

Alternate gene names: 109946643

Gene position: 3162-3914 (Clockwise)

Preceding gene: 109946642

Following gene: 109946646

Centisome position: 0.2

GC content: 37.05

Gene sequence:

>753_bases
ATGAAATTGATTTCATGGAATGTGAATGGTTTGAGAGCTTGCATGACTAAGGGCTTTATGGATTTTTTCAATAGCGTGGA
TGCGGATATTTTTTGCATTCAAGAATCTAAAATGCAACAAGAACAAAACACCTTTGAATTTAAAGGGTATTTTGATTTTT
GGAATTGCGCGATCAAAAAAGGCTATTCTGGGGTGGTAACTTTCACCAAAAAAGAGCCTTTGAGCGTGAGCTATGGTATC
AATATTGAAGAGCATGACAAAGAAGGGCGCATCATCACTTGCGAGTTTGAATCGTTTTATTTAGTGAACATTTATACCCC
CAATTCCCAACAAGCCCTATCCAGGCTCAATTACCGCATGAGTTGGGAAGTAGAGTTTAGAAGATTTTTAAAAGCTTTAG
AGTTGAAAAAACCTGTCATTGTGTGCGGGGATTTGAATGTGGCTCACAATGAAATTGATTTAGAAAACCCTAAAACAAAC
CGAAAAAATGCAGGCTTTAGCGATGAAGAGAGAGGGAAATTCAACGAGCTTTTAAACACCGGTTTTATTGACACTTTCCG
TTATTTTTACCCCAATAAAGAAAAGGCTTATACCTGGTGGAGTTATATGCAACAAGCGAGAGATAAAAATATCGGTTGGC
GCATTGATTATTTTTTATGTTCCAATAGCCTAAAAACGCGTTTAAAAGACGCTTTAATCTATAAAGATATTTTAGGGAGC
GATCATTGTCCGGTAGGGTTGGAATTGGTTTAG

Upstream 100 bases:

>100_bases
TCTGCTCTACCGACTGAGCTATCAAGCCAAGCAAAAGCTGATTATCACATAAAAAAGGTAAAATAAAGCTTAAAGATTAA
AAAATTGAACAAGGATTAAA

Downstream 100 bases:

>100_bases
AGGTAGAAAGTGTGCGAAATAAAAATAGCGAACATTCTCACAAAAGGGGAGTTGTATTCAAAGGCAAAAGGCCATTTGAC
CCCCACTTCTATGCTAGCGT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKLISWNVNGLRACMTKGFMDFFNSVDADIFCIQESKMQQEQNTFEFKGYFDFWNCAIKKGYSGVVTFTKKEPLSVSYGI
NIEEHDKEGRIITCEFESFYLVNIYTPNSQQALSRLNYRMSWEVEFRRFLKALELKKPVIVCGDLNVAHNEIDLENPKTN
RKNAGFSDEERGKFNELLNTGFIDTFRYFYPNKEKAYTWWSYMQQARDKNIGWRIDYFLCSNSLKTRLKDALIYKDILGS
DHCPVGLELV

Sequences:

>Translated_250_residues
MKLISWNVNGLRACMTKGFMDFFNSVDADIFCIQESKMQQEQNTFEFKGYFDFWNCAIKKGYSGVVTFTKKEPLSVSYGI
NIEEHDKEGRIITCEFESFYLVNIYTPNSQQALSRLNYRMSWEVEFRRFLKALELKKPVIVCGDLNVAHNEIDLENPKTN
RKNAGFSDEERGKFNELLNTGFIDTFRYFYPNKEKAYTWWSYMQQARDKNIGWRIDYFLCSNSLKTRLKDALIYKDILGS
DHCPVGLELV
>Mature_250_residues
MKLISWNVNGLRACMTKGFMDFFNSVDADIFCIQESKMQQEQNTFEFKGYFDFWNCAIKKGYSGVVTFTKKEPLSVSYGI
NIEEHDKEGRIITCEFESFYLVNIYTPNSQQALSRLNYRMSWEVEFRRFLKALELKKPVIVCGDLNVAHNEIDLENPKTN
RKNAGFSDEERGKFNELLNTGFIDTFRYFYPNKEKAYTWWSYMQQARDKNIGWRIDYFLCSNSLKTRLKDALIYKDILGS
DHCPVGLELV

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=256, Percent_Identity=50, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI18375503, Length=256, Percent_Identity=50, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI18375501, Length=256, Percent_Identity=50, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI18375507, Length=319, Percent_Identity=28.2131661442006, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71989536, Length=253, Percent_Identity=42.6877470355731, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71989539, Length=143, Percent_Identity=35.6643356643357, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319452, Length=341, Percent_Identity=25.2199413489736, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=48, Blast_Score=260, Evalue=6e-70,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=48, Blast_Score=260, Evalue=7e-70,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29408; Mature: 29408

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGLRACMTKGFMDFFNSVDADIFCIQESKMQQEQNTFEFKGYFDFWNCAIKK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEEEHHHHHHHHHC
GYSGVVTFTKKEPLSVSYGINIEEHDKEGRIITCEFESFYLVNIYTPNSQQALSRLNYRM
CCCEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEEEECCCHHHHHHHCCCEE
SWEVEFRRFLKALELKKPVIVCGDLNVAHNEIDLENPKTNRKNAGFSDEERGKFNELLNT
EEEHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GFIDTFRYFYPNKEKAYTWWSYMQQARDKNIGWRIDYFLCSNSLKTRLKDALIYKDILGS
CHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCC
DHCPVGLELV
CCCCCCCEEC
>Mature Secondary Structure
MKLISWNVNGLRACMTKGFMDFFNSVDADIFCIQESKMQQEQNTFEFKGYFDFWNCAIKK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEEEHHHHHHHHHC
GYSGVVTFTKKEPLSVSYGINIEEHDKEGRIITCEFESFYLVNIYTPNSQQALSRLNYRM
CCCEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEEEECCCHHHHHHHCCCEE
SWEVEFRRFLKALELKKPVIVCGDLNVAHNEIDLENPKTNRKNAGFSDEERGKFNELLNT
EEEHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GFIDTFRYFYPNKEKAYTWWSYMQQARDKNIGWRIDYFLCSNSLKTRLKDALIYKDILGS
CHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCC
DHCPVGLELV
CCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]