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The map label for this gene is metB [H]

Identifier: 108813900

GI number: 108813900

Start: 4291617

End: 4292606

Strand: Reverse

Name: metB [H]

Synonym: YPN_3740

Alternate gene names: 108813900

Gene position: 4292606-4291617 (Counterclockwise)

Preceding gene: 108813903

Following gene: 108813899

Centisome position: 94.66

GC content: 52.22

Gene sequence:

>990_bases
ATGACGCGTAAACAGGCAACAATAGCAGTCCGTAGCGGGTTGAATGATGACGAGCAATACGGCTGCGTTGTCCCCCCGAT
TCACCTTTCCAGTACCTACAATTTTATTGATTTTAATCAGCCGCGCACGCATGACTATTCACGTCGTGGTAATCCAACGC
GTGATGTTGTCCAACGGGCGCTGGCGGAATTGGAAGGGGGGGCCGGTGCCGTCATGACCAGCAGCGGGATGTCGGCGCTT
CATTTGGTTTGCACTACATTCTTACAGCCGGGCGATCTGTTGGTCGCTCCGCATGACTGTTACGGTGGCAGTTACCGTTT
ATTTGACAGCTTGAGCAAGCGTGGTGCGTATCGGGTGTTATTTGTTGATCAGGGCGATGAAGCGGCACTAAACTGCGCAT
TGGCGGAGAAACCGAAGTTGGTCTTGATTGAAACACCGAGTAATCCATTGCTACGGGTTGTTGATATTGCCGCCATCTGC
CAAGCCGCCCGTGCTGCGGGCGCACTGACGGTTTGTGATAACACCTTCCTCAGCCCCGCCTTACAGCAGCCTCTCTCTCT
TGGGGCCGATTTAGTGGTGCACTCCTGTACCAAATATCTCAATGGTCACTCTGATGTGGTGGCTGGTGCTGTTATTGCGA
AAGATCCAGAACTGGTTGTCGAGCTGGCATGGTGGGCAAATAATATTGGTGTAACCGGTGCTGCGTTTGACAGCTATCTA
CTCCTTCGTGGTTTACGCACGTTATCACCACGCATGGCTCAACAGCAGCGTAACGCGGATGACATTGTGCGTTATTTACA
GCAACAGCCTTTAGTGAAAAAGCTGTATCATCCTTCCCTGCCACAACATCCCGGCCACGAAATAGCCTGCCGTCAGCAAT
CAGGTTTTGGTGCAATGCTCAGTTTTGAGCTGGATGGTGATGAGCAGGTCATGCGCCGTTTCCTTTCTGCCCTTGAGCTA
TTTACCTTGGCAGAGTCTTTGGGGGGGTAG

Upstream 100 bases:

>100_bases
TATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCGTAATTTGTGCGTCTACTCTGGCGTAACATTTTCTCGAT
CCCGGCTGGTGGAAATCAAT

Downstream 100 bases:

>100_bases
AAAGCCTGATCTCCCATGCAGCGACCATGACCCACGCGGGTATGGCGGCAGAGGCGCGTATTGCCGCAGGCATTACTGAT
AGTTTGTTGCGTATTTCCGT

Product: hypothetical protein

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAL
HLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNCALAEKPKLVLIETPSNPLLRVVDIAAIC
QAARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAKDPELVVELAWWANNIGVTGAAFDSYL
LLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQSGFGAMLSFELDGDEQVMRRFLSALEL
FTLAESLGG

Sequences:

>Translated_329_residues
MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAL
HLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNCALAEKPKLVLIETPSNPLLRVVDIAAIC
QAARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAKDPELVVELAWWANNIGVTGAAFDSYL
LLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQSGFGAMLSFELDGDEQVMRRFLSALEL
FTLAESLGG
>Mature_328_residues
TRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSALH
LVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNCALAEKPKLVLIETPSNPLLRVVDIAAICQ
AARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAKDPELVVELAWWANNIGVTGAAFDSYLL
LRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQSGFGAMLSFELDGDEQVMRRFLSALELF
TLAESLGG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=327, Percent_Identity=40.3669724770642, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI299473759, Length=327, Percent_Identity=37.6146788990826, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI25453487, Length=326, Percent_Identity=35.8895705521472, Blast_Score=181, Evalue=6e-46,
Organism=Escherichia coli, GI1790375, Length=329, Percent_Identity=84.1945288753799, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1789383, Length=344, Percent_Identity=30.5232558139535, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538055, Length=328, Percent_Identity=45.4268292682927, Blast_Score=264, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17560056, Length=328, Percent_Identity=43.9024390243902, Blast_Score=254, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI71980708, Length=317, Percent_Identity=33.4384858044164, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319307, Length=336, Percent_Identity=37.797619047619, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321254, Length=352, Percent_Identity=33.8068181818182, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6321170, Length=322, Percent_Identity=28.8819875776398, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=343, Percent_Identity=29.4460641399417, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323333, Length=292, Percent_Identity=32.8767123287671, Blast_Score=112, Evalue=9e-26,
Organism=Drosophila melanogaster, GI19922554, Length=327, Percent_Identity=40.9785932721713, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 35780; Mature: 35649

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTRDVVQRA
CCCCHHHHHHHCCCCCCCCCCCCCCCEECCCCCCEEECCCCCCCCCCCCCCCHHHHHHHH
LAELEGGAGAVMTSSGMSALHLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVL
HHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEE
FVDQGDEAALNCALAEKPKLVLIETPSNPLLRVVDIAAICQAARAAGALTVCDNTFLSPA
EEECCCCHHEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHH
LQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAKDPELVVELAWWANNIGVTGAAFDSYL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCHHEEEEEEHHCCCCCCHHHHHHHH
LLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQSGFGAML
HHHHHHHCCHHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEECCCCCCEEE
SFELDGDEQVMRRFLSALELFTLAESLGG
EEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTRDVVQRA
CCCHHHHHHHCCCCCCCCCCCCCCCEECCCCCCEEECCCCCCCCCCCCCCCHHHHHHHH
LAELEGGAGAVMTSSGMSALHLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVL
HHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEE
FVDQGDEAALNCALAEKPKLVLIETPSNPLLRVVDIAAICQAARAAGALTVCDNTFLSPA
EEECCCCHHEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHH
LQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAKDPELVVELAWWANNIGVTGAAFDSYL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCHHEEEEEEHHCCCCCCHHHHHHHH
LLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQSGFGAML
HHHHHHHCCHHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEECCCCCCEEE
SFELDGDEQVMRRFLSALELFTLAESLGG
EEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]