Click here to switch to the map view.

The map label for this gene is alkJ [H]

Identifier: 108801901

GI number: 108801901

Start: 5227751

End: 5229352

Strand: Reverse

Name: alkJ [H]

Synonym: Mmcs_4938

Alternate gene names: 108801901

Gene position: 5229352-5227751 (Counterclockwise)

Preceding gene: 108801903

Following gene: 108801900

Centisome position: 91.66

GC content: 66.92

Gene sequence:

>1602_bases
ATGGCCAGCTACGACTACATCATCACCGGAGCCGGATCGGCCGGTTGCGTCCTGGCCAATCGGCTGTCGGAGGATCCGAG
ACTCAACGTGCTGCTGCTCGAGGCAGGCGGCGGCGATCGCAATCTGTGGTTCCACATCCCCAAGGGTTCGGGAAAGCTCT
TCGAGAGCGAAAAGCACATGTGGCACTACGAGACCACGCCGTTCGGACCCGATCAGCACGTCGAGCAGTGGATGCGCGGG
AAGGCGCTCGGCGGGTCGAGTTCGATCAACGGACTGCTCTACAACCGCGGCAACCGCGCCGACTACGACGGGCTGGAGCG
GTTGGGCAACAAGGGGTGGGGCTGGGATGAGATCCTGCCCATCTTCAAGGGATTCGAGAACAACGAGTTCGGTCCGTCCG
CGACGCGCGGGACCGGCGGCCCGCTGAACATCTCCGTCCCCCGCGACCCCGACCCGCTGTGCGAGGAGATGATCGACGCC
GCGACCCGGATCGGGATGTCCCGCGTCGAGGACATCAACGAATCCGACGCCGAACGCATCGGATACGCGACCTCGACGAT
CCGCAAGGGGCGCCGCGTCAGCGCCGCGACTGCGTTCCTCAAGCCCGCAATGCGGCGCCCAAACCTGACCGTGCGCACCG
GCGCGCTCGTCCACCGGGTGATCATCGAGGGCGGGCGCGCGGCCGGGGTCGAGGTCACGACGCCCAGCGGTGTCGAACGG
CTCCGCGCCACCCGCGAGGTGATCGTGTCGATGGGCAGCCTCAACTCGCCGAAGCTGCTCCAGCTCTCCGGTATCGGGCC
ACGCGAGGTGCTCTCGGCCGCCGGGGTGGAGGTCCGCCTGGAACGCGACAACGTCGGTCGTGGACTGCGTGAACACCGTT
GCGCGACTCTGCGTTACGGGCTCAACGAGGATCTCGGTTACAACAGGTACCTCGCAACGAGTATGGGGCAGGCGCTCACC
GGGATGAAATACCTCGCCACACGCAAGGGGCCACTCGCCGCGCCGTCGTTCGACGTCGTCGGCTTCGTGAAAACGCGGCC
CGACGAAGAACGCCCCGACGGGCAGGTGATGATGGGCCCGTACACGTTGCCGCCCTACAACGTCGGCGAACCCGTCTCCA
TCCAGCGCGAGCCGGGCGTGTCCTGCCTCGGCATGGTGTTGCGGCCGACGTCCGAGGGGTATATCGAGATCACCTCGGCC
GATCCCGCCGCGGCGCTGAGGATCAACCCCAACTATCTGGGCACCGACTACGACCGCGAGACGACGGCCGGTCTACTCCG
CAGGATGCGTGCGATCTTCGAGCAGTCACCGATCGCCGGCCGCATCAGCCACGAGACCTATCCTGGTCCGGGGGTGCAGA
GCGATGACCAACTCGTCGACGCCGCGCTCGACGGGGGTTACTGCGGCTACCACGCCGTCGGGACCTGCGCGATGGGGCCC
AGCGACCACGACGTGGTCGACCATCAGCTCCGGCTCAGGGGAGTGGACGGACTGCGCGTCGTCGACTGTTCGGTGATGCC
GACGATCGTGGCCGGCAACCTCAACGGCCCGATCATGGCAATGGCCTGGCGGGCAGCGGATTTCATCCTGCACGGGTGCT
GA

Upstream 100 bases:

>100_bases
GACAGCATCGGCGCAATCGGGGGTGAAACGGGAAGTTGCACCCAGCTATTGTCGCCGAGACACGTACTATACGGCCGTAC
AGCGCGACGAGAGGCGACCC

Downstream 100 bases:

>100_bases
ACCGGCCGTGTGTGCTATACATTTGTATAGCGGTGGCGAGGAGTAGTGGTGAGTCGAGTAGCGGTGGTGACCGGCGGCGC
GTCGGGCCTGGGCGAGGCGA

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MASYDYIITGAGSAGCVLANRLSEDPRLNVLLLEAGGGDRNLWFHIPKGSGKLFESEKHMWHYETTPFGPDQHVEQWMRG
KALGGSSSINGLLYNRGNRADYDGLERLGNKGWGWDEILPIFKGFENNEFGPSATRGTGGPLNISVPRDPDPLCEEMIDA
ATRIGMSRVEDINESDAERIGYATSTIRKGRRVSAATAFLKPAMRRPNLTVRTGALVHRVIIEGGRAAGVEVTTPSGVER
LRATREVIVSMGSLNSPKLLQLSGIGPREVLSAAGVEVRLERDNVGRGLREHRCATLRYGLNEDLGYNRYLATSMGQALT
GMKYLATRKGPLAAPSFDVVGFVKTRPDEERPDGQVMMGPYTLPPYNVGEPVSIQREPGVSCLGMVLRPTSEGYIEITSA
DPAAALRINPNYLGTDYDRETTAGLLRRMRAIFEQSPIAGRISHETYPGPGVQSDDQLVDAALDGGYCGYHAVGTCAMGP
SDHDVVDHQLRLRGVDGLRVVDCSVMPTIVAGNLNGPIMAMAWRAADFILHGC

Sequences:

>Translated_533_residues
MASYDYIITGAGSAGCVLANRLSEDPRLNVLLLEAGGGDRNLWFHIPKGSGKLFESEKHMWHYETTPFGPDQHVEQWMRG
KALGGSSSINGLLYNRGNRADYDGLERLGNKGWGWDEILPIFKGFENNEFGPSATRGTGGPLNISVPRDPDPLCEEMIDA
ATRIGMSRVEDINESDAERIGYATSTIRKGRRVSAATAFLKPAMRRPNLTVRTGALVHRVIIEGGRAAGVEVTTPSGVER
LRATREVIVSMGSLNSPKLLQLSGIGPREVLSAAGVEVRLERDNVGRGLREHRCATLRYGLNEDLGYNRYLATSMGQALT
GMKYLATRKGPLAAPSFDVVGFVKTRPDEERPDGQVMMGPYTLPPYNVGEPVSIQREPGVSCLGMVLRPTSEGYIEITSA
DPAAALRINPNYLGTDYDRETTAGLLRRMRAIFEQSPIAGRISHETYPGPGVQSDDQLVDAALDGGYCGYHAVGTCAMGP
SDHDVVDHQLRLRGVDGLRVVDCSVMPTIVAGNLNGPIMAMAWRAADFILHGC
>Mature_532_residues
ASYDYIITGAGSAGCVLANRLSEDPRLNVLLLEAGGGDRNLWFHIPKGSGKLFESEKHMWHYETTPFGPDQHVEQWMRGK
ALGGSSSINGLLYNRGNRADYDGLERLGNKGWGWDEILPIFKGFENNEFGPSATRGTGGPLNISVPRDPDPLCEEMIDAA
TRIGMSRVEDINESDAERIGYATSTIRKGRRVSAATAFLKPAMRRPNLTVRTGALVHRVIIEGGRAAGVEVTTPSGVERL
RATREVIVSMGSLNSPKLLQLSGIGPREVLSAAGVEVRLERDNVGRGLREHRCATLRYGLNEDLGYNRYLATSMGQALTG
MKYLATRKGPLAAPSFDVVGFVKTRPDEERPDGQVMMGPYTLPPYNVGEPVSIQREPGVSCLGMVLRPTSEGYIEITSAD
PAAALRINPNYLGTDYDRETTAGLLRRMRAIFEQSPIAGRISHETYPGPGVQSDDQLVDAALDGGYCGYHAVGTCAMGPS
DHDVVDHQLRLRGVDGLRVVDCSVMPTIVAGNLNGPIMAMAWRAADFILHGC

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=539, Percent_Identity=37.6623376623377, Blast_Score=312, Evalue=6e-85,
Organism=Escherichia coli, GI1786503, Length=539, Percent_Identity=35.2504638218924, Blast_Score=283, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17532301, Length=554, Percent_Identity=33.3935018050542, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24642055, Length=571, Percent_Identity=35.9019264448336, Blast_Score=278, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24642042, Length=571, Percent_Identity=35.0262697022767, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24642059, Length=573, Percent_Identity=35.0785340314136, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24642039, Length=567, Percent_Identity=32.2751322751323, Blast_Score=248, Evalue=1e-65,
Organism=Drosophila melanogaster, GI45551458, Length=567, Percent_Identity=32.9805996472663, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI45549471, Length=566, Percent_Identity=33.0388692579505, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17137792, Length=550, Percent_Identity=34, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI18859995, Length=566, Percent_Identity=32.3321554770318, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24642048, Length=575, Percent_Identity=32.1739130434783, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24642037, Length=571, Percent_Identity=32.0490367775832, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24650267, Length=575, Percent_Identity=31.8260869565217, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24642051, Length=572, Percent_Identity=30.9440559440559, Blast_Score=223, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24642035, Length=572, Percent_Identity=30.4195804195804, Blast_Score=215, Evalue=8e-56,
Organism=Drosophila melanogaster, GI18859993, Length=569, Percent_Identity=29.3497363796134, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24642057, Length=585, Percent_Identity=31.6239316239316, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24645930, Length=588, Percent_Identity=25.5102040816327, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 57826; Mature: 57695

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASYDYIITGAGSAGCVLANRLSEDPRLNVLLLEAGGGDRNLWFHIPKGSGKLFESEKHM
CCCCCEEEECCCCCCHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCEECCCCE
WHYETTPFGPDQHVEQWMRGKALGGSSSINGLLYNRGNRADYDGLERLGNKGWGWDEILP
EEEECCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCHHHHHH
IFKGFENNEFGPSATRGTGGPLNISVPRDPDPLCEEMIDAATRIGMSRVEDINESDAERI
HHCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GYATSTIRKGRRVSAATAFLKPAMRRPNLTVRTGALVHRVIIEGGRAAGVEVTTPSGVER
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCEEEECCHHHHH
LRATREVIVSMGSLNSPKLLQLSGIGPREVLSAAGVEVRLERDNVGRGLREHRCATLRYG
HHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCCCEEEEECCCCCCCHHHHCCEEEECC
LNEDLGYNRYLATSMGQALTGMKYLATRKGPLAAPSFDVVGFVKTRPDEERPDGQVMMGP
CCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECC
YTLPPYNVGEPVSIQREPGVSCLGMVLRPTSEGYIEITSADPAAALRINPNYLGTDYDRE
EECCCCCCCCCCEEECCCCCCEEHEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCH
TTAGLLRRMRAIFEQSPIAGRISHETYPGPGVQSDDQLVDAALDGGYCGYHAVGTCAMGP
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHEEECCCC
SDHDVVDHQLRLRGVDGLRVVDCSVMPTIVAGNLNGPIMAMAWRAADFILHGC
CCCCHHCCEEEEECCCCEEEEECCCCCEEEECCCCCCEEEEHHHHHHHHEECC
>Mature Secondary Structure 
ASYDYIITGAGSAGCVLANRLSEDPRLNVLLLEAGGGDRNLWFHIPKGSGKLFESEKHM
CCCCEEEECCCCCCHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCEECCCCE
WHYETTPFGPDQHVEQWMRGKALGGSSSINGLLYNRGNRADYDGLERLGNKGWGWDEILP
EEEECCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCHHHHHH
IFKGFENNEFGPSATRGTGGPLNISVPRDPDPLCEEMIDAATRIGMSRVEDINESDAERI
HHCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GYATSTIRKGRRVSAATAFLKPAMRRPNLTVRTGALVHRVIIEGGRAAGVEVTTPSGVER
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCEEEECCHHHHH
LRATREVIVSMGSLNSPKLLQLSGIGPREVLSAAGVEVRLERDNVGRGLREHRCATLRYG
HHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCCCEEEEECCCCCCCHHHHCCEEEECC
LNEDLGYNRYLATSMGQALTGMKYLATRKGPLAAPSFDVVGFVKTRPDEERPDGQVMMGP
CCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECC
YTLPPYNVGEPVSIQREPGVSCLGMVLRPTSEGYIEITSADPAAALRINPNYLGTDYDRE
EECCCCCCCCCCEEECCCCCCEEHEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCH
TTAGLLRRMRAIFEQSPIAGRISHETYPGPGVQSDDQLVDAALDGGYCGYHAVGTCAMGP
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHEEECCCC
SDHDVVDHQLRLRGVDGLRVVDCSVMPTIVAGNLNGPIMAMAWRAADFILHGC
CCCCHHCCEEEEECCCCEEEEECCCCCEEEECCCCCCEEEEHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]