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The map label for this gene is lysS [H]

Identifier: 108801721

GI number: 108801721

Start: 5035062

End: 5036600

Strand: Reverse

Name: lysS [H]

Synonym: Mmcs_4758

Alternate gene names: 108801721

Gene position: 5036600-5035062 (Counterclockwise)

Preceding gene: 108801723

Following gene: 108801720

Centisome position: 88.28

GC content: 64.65

Gene sequence:

>1539_bases
GTGACACCGACCGGCGGCGACAACACGCCCGAGAACAACGCGCCCGATAACGACCCTGACATCCCCGAGCAGTTCCGCAT
TCGTCAGGCCAAGCGGGAACGTCTGCTGGCCGAGGGTCGCGACCCCTACCCGGTGGACGTGGACCGCACGCACACGCTCG
CCGAAGTGCGCGCCGCTTTCCCCGACCTGCCGGTCGACTCGCAGACCGGCGAGATCGTCGGCGTCGCCGGGCGCGTCGTA
TTCGCCCGCAACTCGGGGAAGCTGTGTTTCGCGACCTTGCAGGAGGGCGACGGTACGCAACTACAGGCCATGATCAGCCT
GGCCGAGGTGGGGCAGGAATCGCTCGACAGCTGGAAAGCCGACGTCGACCTGGGTGACATCGTGTCCGTCCACGGCCAGG
TCATCAGTTCCCGTCGCGGTGAGCTCTCTGTGCTCGCCGATTCCTGGCAAATCATCTCCAAGGCGCTGCGGCCGCTTCCG
GTCGCGCACAAGGAGATGAGCGAGGAGGCACGGGTGCGGCAGCGCTACGTCGACCTCATCGTGCGTCCGGAGGCCCGCAC
CATCGCACGTCAACGCGTGGCCGTCGTGCGTGCGCTCCGCAACGCGCTCGAGCGGCGCGGCTTCCTCGAACTCGAGACGC
CGATGCTGCAGACCCTGGCCGGCGGTGCGGCGGCCAGGCCGTTCGTCACCCACTCCAATGCACTCGATGTGGACCTATAC
CTGCGTATCGCGCCGGAGCTGTTCCTCAAGCGTGCCCTGGTCGGTGGTTTCGAGAAGGTCTTCGAGCTGAATCGCAACTT
CCGAAACGAAGGTATCGATTCCACGCATTCTCCCGAATTCGCGATGCTGGAGACTTATCAGGCGTATGGCGACTACAACG
ATTCCGCGGTCGTGACGCGGGAACTAATTCAGGAAGTCGCCGACGAGGCGATCGGGACCCGTCAAGTGCCATTGGCGGAT
GGCACCGTGTACGACCTGGACGGTGAGTGGGAGTCGTTGGAAATGTACCCCTCGCTGTCCGCGGCGCTGGGTGAGGAGAT
CACCCCGCAGACCCCCGCCGACGAACTCTGGCGGATCGCCGACCGGCTCGGTGTGGAGATTCCCCGTGACCGCGGATACG
GCCACGGGAAATTGATCGAGGAACTGTGGGAGCACACCGTCGGCGAGGATCTCTGGGCGCCGACCTTCGTGCGGGACTTC
CCGGTGGAGACGACCCCGCTCACCCGGTCGCACCGCAGCGTTCCCGGCGTCACCGAGAAGTGGGATCTCTATGTCCGCAA
GTTCGAGTTGGCGACCGGCTATTCCGAACTCATCGATCCGATAATTCAGCGCGAGCGATTCGAGGAGCAGGCGCGAGCGG
CCGCTGCCGGTGACGACGAGGCAATGGTTCTCGACGAGGATTTCCTGGCGGCATTGGAGTACGGCATGCCGCCGTCCACC
GGTACCGGAATGGGCATCGACCGGTTGCTGATGGCGCTGACTGGACTGTCAATTAGAGAGACAATTCTCTTCCCGATTGT
TCGGCGGCACGGCGGCTGA

Upstream 100 bases:

>100_bases
GTCGATGGGGCTGCGCATTCGTTCATTAAAGGCGGATGGACACCGGGAGGCGATCGCGGCGTGCAGGGGGTTCTCGTGAG
CAGCGTTAAGCTGGCACCCC

Downstream 100 bases:

>100_bases
ACTCCTGGGATAGGAGCGTCGTTGAATAGTGTAGATTTATATGGCACATTGGAGGCCGAGAATCCGGTAGCCAAAACTAT
CCACCAAGCGCAGGTAGAAG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTPTGGDNTPENNAPDNDPDIPEQFRIRQAKRERLLAEGRDPYPVDVDRTHTLAEVRAAFPDLPVDSQTGEIVGVAGRVV
FARNSGKLCFATLQEGDGTQLQAMISLAEVGQESLDSWKADVDLGDIVSVHGQVISSRRGELSVLADSWQIISKALRPLP
VAHKEMSEEARVRQRYVDLIVRPEARTIARQRVAVVRALRNALERRGFLELETPMLQTLAGGAAARPFVTHSNALDVDLY
LRIAPELFLKRALVGGFEKVFELNRNFRNEGIDSTHSPEFAMLETYQAYGDYNDSAVVTRELIQEVADEAIGTRQVPLAD
GTVYDLDGEWESLEMYPSLSAALGEEITPQTPADELWRIADRLGVEIPRDRGYGHGKLIEELWEHTVGEDLWAPTFVRDF
PVETTPLTRSHRSVPGVTEKWDLYVRKFELATGYSELIDPIIQRERFEEQARAAAAGDDEAMVLDEDFLAALEYGMPPST
GTGMGIDRLLMALTGLSIRETILFPIVRRHGG

Sequences:

>Translated_512_residues
MTPTGGDNTPENNAPDNDPDIPEQFRIRQAKRERLLAEGRDPYPVDVDRTHTLAEVRAAFPDLPVDSQTGEIVGVAGRVV
FARNSGKLCFATLQEGDGTQLQAMISLAEVGQESLDSWKADVDLGDIVSVHGQVISSRRGELSVLADSWQIISKALRPLP
VAHKEMSEEARVRQRYVDLIVRPEARTIARQRVAVVRALRNALERRGFLELETPMLQTLAGGAAARPFVTHSNALDVDLY
LRIAPELFLKRALVGGFEKVFELNRNFRNEGIDSTHSPEFAMLETYQAYGDYNDSAVVTRELIQEVADEAIGTRQVPLAD
GTVYDLDGEWESLEMYPSLSAALGEEITPQTPADELWRIADRLGVEIPRDRGYGHGKLIEELWEHTVGEDLWAPTFVRDF
PVETTPLTRSHRSVPGVTEKWDLYVRKFELATGYSELIDPIIQRERFEEQARAAAAGDDEAMVLDEDFLAALEYGMPPST
GTGMGIDRLLMALTGLSIRETILFPIVRRHGG
>Mature_511_residues
TPTGGDNTPENNAPDNDPDIPEQFRIRQAKRERLLAEGRDPYPVDVDRTHTLAEVRAAFPDLPVDSQTGEIVGVAGRVVF
ARNSGKLCFATLQEGDGTQLQAMISLAEVGQESLDSWKADVDLGDIVSVHGQVISSRRGELSVLADSWQIISKALRPLPV
AHKEMSEEARVRQRYVDLIVRPEARTIARQRVAVVRALRNALERRGFLELETPMLQTLAGGAAARPFVTHSNALDVDLYL
RIAPELFLKRALVGGFEKVFELNRNFRNEGIDSTHSPEFAMLETYQAYGDYNDSAVVTRELIQEVADEAIGTRQVPLADG
TVYDLDGEWESLEMYPSLSAALGEEITPQTPADELWRIADRLGVEIPRDRGYGHGKLIEELWEHTVGEDLWAPTFVRDFP
VETTPLTRSHRSVPGVTEKWDLYVRKFELATGYSELIDPIIQRERFEEQARAAAAGDDEAMVLDEDFLAALEYGMPPSTG
TGMGIDRLLMALTGLSIRETILFPIVRRHGG

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=511, Percent_Identity=39.3346379647749, Blast_Score=332, Evalue=8e-91,
Organism=Homo sapiens, GI194272210, Length=511, Percent_Identity=39.3346379647749, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI45439306, Length=472, Percent_Identity=23.3050847457627, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1790571, Length=499, Percent_Identity=41.2825651302605, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1789256, Length=503, Percent_Identity=39.7614314115308, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI87082379, Length=330, Percent_Identity=32.1212121212121, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788173, Length=233, Percent_Identity=28.3261802575107, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=517, Percent_Identity=38.6847195357834, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17535927, Length=517, Percent_Identity=38.4912959381045, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI71994340, Length=506, Percent_Identity=38.9328063241107, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17551876, Length=471, Percent_Identity=24.8407643312102, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=520, Percent_Identity=37.6923076923077, Blast_Score=308, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6324256, Length=464, Percent_Identity=28.2327586206897, Blast_Score=156, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24640849, Length=513, Percent_Identity=38.9863547758285, Blast_Score=335, Evalue=7e-92,
Organism=Drosophila melanogaster, GI24640851, Length=513, Percent_Identity=38.9863547758285, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI17136276, Length=483, Percent_Identity=24.0165631469979, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56986; Mature: 56855

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTGGDNTPENNAPDNDPDIPEQFRIRQAKRERLLAEGRDPYPVDVDRTHTLAEVRAAF
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
PDLPVDSQTGEIVGVAGRVVFARNSGKLCFATLQEGDGTQLQAMISLAEVGQESLDSWKA
CCCCCCCCCCCEEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHCC
DVDLGDIVSVHGQVISSRRGELSVLADSWQIISKALRPLPVAHKEMSEEARVRQRYVDLI
CCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VRPEARTIARQRVAVVRALRNALERRGFLELETPMLQTLAGGAAARPFVTHSNALDVDLY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCCCEEECCCCCCEEHH
LRIAPELFLKRALVGGFEKVFELNRNFRNEGIDSTHSPEFAMLETYQAYGDYNDSAVVTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHH
ELIQEVADEAIGTRQVPLADGTVYDLDGEWESLEMYPSLSAALGEEITPQTPADELWRIA
HHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEECCCCHHHHHCCCCCCCCCHHHHHHHH
DRLGVEIPRDRGYGHGKLIEELWEHTVGEDLWAPTFVRDFPVETTPLTRSHRSVPGVTEK
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHCCCHHHHHCCCCCCCCCCCCCCCCCCCHH
WDLYVRKFELATGYSELIDPIIQRERFEEQARAAAAGDDEAMVLDEDFLAALEYGMPPST
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCC
GTGMGIDRLLMALTGLSIRETILFPIVRRHGG
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPTGGDNTPENNAPDNDPDIPEQFRIRQAKRERLLAEGRDPYPVDVDRTHTLAEVRAAF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
PDLPVDSQTGEIVGVAGRVVFARNSGKLCFATLQEGDGTQLQAMISLAEVGQESLDSWKA
CCCCCCCCCCCEEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHCC
DVDLGDIVSVHGQVISSRRGELSVLADSWQIISKALRPLPVAHKEMSEEARVRQRYVDLI
CCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VRPEARTIARQRVAVVRALRNALERRGFLELETPMLQTLAGGAAARPFVTHSNALDVDLY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCCCEEECCCCCCEEHH
LRIAPELFLKRALVGGFEKVFELNRNFRNEGIDSTHSPEFAMLETYQAYGDYNDSAVVTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHH
ELIQEVADEAIGTRQVPLADGTVYDLDGEWESLEMYPSLSAALGEEITPQTPADELWRIA
HHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEECCCCHHHHHCCCCCCCCCHHHHHHHH
DRLGVEIPRDRGYGHGKLIEELWEHTVGEDLWAPTFVRDFPVETTPLTRSHRSVPGVTEK
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHCCCHHHHHCCCCCCCCCCCCCCCCCCCHH
WDLYVRKFELATGYSELIDPIIQRERFEEQARAAAAGDDEAMVLDEDFLAALEYGMPPST
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCC
GTGMGIDRLLMALTGLSIRETILFPIVRRHGG
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA