Click here to switch to the map view.

The map label for this gene is icaB [H]

Identifier: 108801605

GI number: 108801605

Start: 4915573

End: 4916946

Strand: Reverse

Name: icaB [H]

Synonym: Mmcs_4642

Alternate gene names: 108801605

Gene position: 4916946-4915573 (Counterclockwise)

Preceding gene: 108801606

Following gene: 108801602

Centisome position: 86.18

GC content: 69.8

Gene sequence:

>1374_bases
ATGACGGCGACGGAGGACCAGCCCGACCCCGCCGCGGATCCGCGACACCATCTCGTCGTCACGCCGGAGGCGTTCGATGC
GCAGCTCACCGCGCTGCGGGCCGCGGGCTACACCTCCATCACGTCCGATCAGTACGTGGACTACCTGGCAGGTGGCGAGG
TCCCGGAACGGTCGGTGCTCCTCACGTTCGACGACGGCACGCACGGACTGTGGACATACGCGGACAAGATCCTCGAGCGA
CACGGGATGCACGCGGTGTCGTTCCTCATCACCGGCAATGTCGGCGCGAAACGGCCGTACTACCTGTCGTGGCAGGAGAT
CGAGCGGATGGCCGACTCCGGCCGGTGGGACTTCCAGTCGCACACCCGGAAGATGCACGCCCGGCTGCCGATCGATGCCG
CGGGCACCCTGGCATCCGAGATGACGCACCGCCGTTGGCTTTTCGAGAAGAACCGGCCAGAGACGCTCGACGAGTTCGAA
GGCAAGATCAGAACCGACCTGCTGGCGTCGGTACAGGACATCGTCGACCACGGACTGCCGCGTCCGACACTGTTCGCCTT
CCCGTTCTCCGACGGATACGACGACGACCGCGCCTCGAGCGACCCACAGGCTGCCGCGACCGCGCTGACGGTCGTCCGGG
AGATCTTCGCGGGCGCGTTCAACAACGCACCCCCGCAGCCACTGCCCGCCGGTGCCCGGGCCGCGGCGGTGGGAATGACG
GGCCGCATCGAGCTGACCGTCGACTCCACCGTCGAGGGGTTGCTCGAGAGCGTCCGCGCGAAAACGCCTGTCGCCCCGGG
CCATGCTGCACCGTCACGCCGCCCCGATCTGTGGACACGGCTGAGCGACGACACCGAGGCCCCGCTGCGCATACAGGGGA
GGGGCGTTCGCTTCGACGGGCCGGGCCGGTGGTCGGGTGCCGCGTACGGACGTGAGGCGACGGCGGACTGGGCGTCGTAC
ACGGCGACGGTCACTGTGGGCGGGTTGGCGGCACGTGGTGTCGAGAACGGGGCGCTGATCGCCAGGGTCGGCACCGGCGA
AGAGGTGTCGGTCCAGGTCAGCGCGAAGTACGCCCGGGTGTCGGTCGGGCTCGGTCCGGCGTGGAAGGTGTTGCGTGACC
TGCCGTTGCGGCCCGGTGACCGGCACACCGTCACCATGGAGGTGTCGCCGACGGCCATCGGCGTGGTCGTGGACGGCTCG
GCCCGGATGTCGGTCCCGGCCGGCGGTGGGCCCGCCGAGTACGGCGGTATCGGGCTGAGCAGTTCGCGGACCACGGAGGC
CGCACCGTGGCCGGTCTTCACCGACCTCTCGGTCGCCCCGGGCCGGGACGTTCCGAATGTCAAAGCGGGGGTAGGGGTTC
CGACCCGAAGCTGA

Upstream 100 bases:

>100_bases
GTCACTCGCGGCCGACCAGGACGCGCTCGCAGAGACCCTGCGATCCGAACTGCCTGACCGCGCGGCCCCGGTCGTCCTCG
CCTACCACGACATCCGGCCG

Downstream 100 bases:

>100_bases
CGCGCAGATCGTCGAGGCGCAGCTCGGCGTTGTCGCCCCGTATCCCGACCGAGCCCGCATGCAGCGGCGGGCAGCCTGTA
CCGCTGTCGACCGCCGTCAC

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: PNAG N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase; Biofilm polysaccharide intercellular adhesin deacetylase; Biofilm PIA deacetylase; Intercellular adhesion protein B [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTATEDQPDPAADPRHHLVVTPEAFDAQLTALRAAGYTSITSDQYVDYLAGGEVPERSVLLTFDDGTHGLWTYADKILER
HGMHAVSFLITGNVGAKRPYYLSWQEIERMADSGRWDFQSHTRKMHARLPIDAAGTLASEMTHRRWLFEKNRPETLDEFE
GKIRTDLLASVQDIVDHGLPRPTLFAFPFSDGYDDDRASSDPQAAATALTVVREIFAGAFNNAPPQPLPAGARAAAVGMT
GRIELTVDSTVEGLLESVRAKTPVAPGHAAPSRRPDLWTRLSDDTEAPLRIQGRGVRFDGPGRWSGAAYGREATADWASY
TATVTVGGLAARGVENGALIARVGTGEEVSVQVSAKYARVSVGLGPAWKVLRDLPLRPGDRHTVTMEVSPTAIGVVVDGS
ARMSVPAGGGPAEYGGIGLSSSRTTEAAPWPVFTDLSVAPGRDVPNVKAGVGVPTRS

Sequences:

>Translated_457_residues
MTATEDQPDPAADPRHHLVVTPEAFDAQLTALRAAGYTSITSDQYVDYLAGGEVPERSVLLTFDDGTHGLWTYADKILER
HGMHAVSFLITGNVGAKRPYYLSWQEIERMADSGRWDFQSHTRKMHARLPIDAAGTLASEMTHRRWLFEKNRPETLDEFE
GKIRTDLLASVQDIVDHGLPRPTLFAFPFSDGYDDDRASSDPQAAATALTVVREIFAGAFNNAPPQPLPAGARAAAVGMT
GRIELTVDSTVEGLLESVRAKTPVAPGHAAPSRRPDLWTRLSDDTEAPLRIQGRGVRFDGPGRWSGAAYGREATADWASY
TATVTVGGLAARGVENGALIARVGTGEEVSVQVSAKYARVSVGLGPAWKVLRDLPLRPGDRHTVTMEVSPTAIGVVVDGS
ARMSVPAGGGPAEYGGIGLSSSRTTEAAPWPVFTDLSVAPGRDVPNVKAGVGVPTRS
>Mature_456_residues
TATEDQPDPAADPRHHLVVTPEAFDAQLTALRAAGYTSITSDQYVDYLAGGEVPERSVLLTFDDGTHGLWTYADKILERH
GMHAVSFLITGNVGAKRPYYLSWQEIERMADSGRWDFQSHTRKMHARLPIDAAGTLASEMTHRRWLFEKNRPETLDEFEG
KIRTDLLASVQDIVDHGLPRPTLFAFPFSDGYDDDRASSDPQAAATALTVVREIFAGAFNNAPPQPLPAGARAAAVGMTG
RIELTVDSTVEGLLESVRAKTPVAPGHAAPSRRPDLWTRLSDDTEAPLRIQGRGVRFDGPGRWSGAAYGREATADWASYT
ATVTVGGLAARGVENGALIARVGTGEEVSVQVSAKYARVSVGLGPAWKVLRDLPLRPGDRHTVTMEVSPTAIGVVVDGSA
RMSVPAGGGPAEYGGIGLSSSRTTEAAPWPVFTDLSVAPGRDVPNVKAGVGVPTRS

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide. In fact, the IcaB deacetylase converts 15 to 20% of the GlcNAc residues of PNAG to glucosamine. N-deacetylation is cr

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Secreted, cell wall. Note=Attached to the cell surface [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=198, Percent_Identity=28.2828282828283, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786322, Length=111, Percent_Identity=32.4324324324324, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 48885; Mature: 48754

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATEDQPDPAADPRHHLVVTPEAFDAQLTALRAAGYTSITSDQYVDYLAGGEVPERSVL
CCCCCCCCCCCCCCCCEEEECCCHHCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEE
LTFDDGTHGLWTYADKILERHGMHAVSFLITGNVGAKRPYYLSWQEIERMADSGRWDFQS
EEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEECHHHHHHHHHCCCCCHHH
HTRKMHARLPIDAAGTLASEMTHRRWLFEKNRPETLDEFEGKIRTDLLASVQDIVDHGLP
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCC
RPTLFAFPFSDGYDDDRASSDPQAAATALTVVREIFAGAFNNAPPQPLPAGARAAAVGMT
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
GRIELTVDSTVEGLLESVRAKTPVAPGHAAPSRRPDLWTRLSDDTEAPLRIQGRGVRFDG
EEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEECCEEEECC
PGRWSGAAYGREATADWASYTATVTVGGLAARGVENGALIARVGTGEEVSVQVSAKYARV
CCCCCCCCCCCCCCCCHHHEEEEEEECCHHHCCCCCCEEEEEECCCCEEEEEEEEEEEEE
SVGLGPAWKVLRDLPLRPGDRHTVTMEVSPTAIGVVVDGSARMSVPAGGGPAEYGGIGLS
EECCCHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEEECCCEEECCCCCCCCCCCCCCCC
SSRTTEAAPWPVFTDLSVAPGRDVPNVKAGVGVPTRS
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TATEDQPDPAADPRHHLVVTPEAFDAQLTALRAAGYTSITSDQYVDYLAGGEVPERSVL
CCCCCCCCCCCCCCCEEEECCCHHCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEE
LTFDDGTHGLWTYADKILERHGMHAVSFLITGNVGAKRPYYLSWQEIERMADSGRWDFQS
EEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEECHHHHHHHHHCCCCCHHH
HTRKMHARLPIDAAGTLASEMTHRRWLFEKNRPETLDEFEGKIRTDLLASVQDIVDHGLP
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCC
RPTLFAFPFSDGYDDDRASSDPQAAATALTVVREIFAGAFNNAPPQPLPAGARAAAVGMT
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
GRIELTVDSTVEGLLESVRAKTPVAPGHAAPSRRPDLWTRLSDDTEAPLRIQGRGVRFDG
EEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEECCEEEECC
PGRWSGAAYGREATADWASYTATVTVGGLAARGVENGALIARVGTGEEVSVQVSAKYARV
CCCCCCCCCCCCCCCCHHHEEEEEEECCHHHCCCCCCEEEEEECCCCEEEEEEEEEEEEE
SVGLGPAWKVLRDLPLRPGDRHTVTMEVSPTAIGVVVDGSARMSVPAGGGPAEYGGIGLS
EECCCHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEEECCCEEECCCCCCCCCCCCCCCC
SSRTTEAAPWPVFTDLSVAPGRDVPNVKAGVGVPTRS
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA