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The map label for this gene is fadE12 [H]

Identifier: 108801365

GI number: 108801365

Start: 4680592

End: 4681749

Strand: Reverse

Name: fadE12 [H]

Synonym: Mmcs_4402

Alternate gene names: 108801365

Gene position: 4681749-4680592 (Counterclockwise)

Preceding gene: 108801366

Following gene: 108801364

Centisome position: 82.06

GC content: 69.08

Gene sequence:

>1158_bases
ATGAGCATGGACTTCGACATGGGGCCGCGCGCCGCCGAATTGCGCAGCCGCCTGAGGGAGCTCGTCAGCGAGCACGTTCC
CGAGCACTTCCTGGGCGCGTTCACCGACGACCCGGCCGATCTGGAGACCGCACAACGGTTCTGCCGCCTGCTCGCCGAAC
AGGATCTGCTGTGCCTGGCGTGGCCGCAGGAGTTCGGCGGCGGGGGAGCGTCGGTGTGGGAGCAGACCGTCGTACGCGAG
GAGATGTGGGCCCACCACGAACCGCGCGGCGCCCAGTACATGGGTGTCAACTGGGTCGGGCCGATCATCATGCGGCACGG
CACCGAGGAGCAGCAGCGCAGACATCTGCCGCCGATCGCGCGCGGTGAGGTGATCTGGTGTCAGGGTTTCTCCGAACCCG
AGGCCGGGTCCGATCTCGCGTCGCTGCGCACCACCGCGCGTCGTGACGGGGACGGGTGGCTGATCAGCGGGCAGAAGATC
TGGACGTCCTACGCGACGATGGCGCAGTGGTGCTTCCTGCTGGCACGGACCTCCAGGGGTGAGCGCAAACAGCAGGGCCA
GACGATCTTCCTGGTGCCGATGGACGATCCGGCGATCCAGGTCCGGCCGATCCGCGCCATGCTCGGCCCGCACCACCTCA
ACGAGGTGTTCTTCGACGATCTGCGGGTGACCGAGGCCGACGTGCTGGGCACCGTGGACGCGGGCTGGTCGATCGTGCAG
GACGTGATGGCGTTCGAACGGGTCGGCATCGCCCGCTACGCCCGGTGTGAGCGGCTGCTCGCTGCTGCGCCAACGGTTCT
CGGCGATCGCTGGGGCGACCTGTCCGAGGAGCTGCGCGGCCGCTGGGTGCGCATGCTCACCCACTGCCGCCGCGCGCGGC
TGATGGCCTACCGGGTGGTGTCGCTGCAGAGCAGCGGACGCATCCAGCCCGGGGACGCCGCCGCCTACCGAATCGCCGTG
ACCAAACTCGATCAGGACAGCGCGGAGGTGCTGATGGACATCTCGGCCGAGGTGTCCCGCGACGATCCGCGGGCGGCCTG
GTTCCTCGGCGAGGTCGAGGACCACTGGCGGTACTCGCAGGCAGCGACCGTGTCCTCGGGCAGCATCGAGATGCAGCGCA
TCCTGCTGTCTCGTGCCCTGCTGGCGGCGGCGAAATGA

Upstream 100 bases:

>100_bases
TCCTCGACGAGCTGGGCCTGAGCCGCGCCGAGCCTCCGGTTGTTGACGGGAATCCCGCGGATCTCGTCGACAACCGTAGT
GTCGGCGCCGCGGAGCTGGG

Downstream 100 bases:

>100_bases
TCCTCGACCTCGGCGACGACGCCACCGAATACGGCCGCCAGGCGCTGCGGGCGTTCGAGACCGCGGGCGGTGACCTGCTG
GTGCAGCAGGCGGAAGCCAA

Product: acyl-CoA dehydrogenase-like protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSMDFDMGPRAAELRSRLRELVSEHVPEHFLGAFTDDPADLETAQRFCRLLAEQDLLCLAWPQEFGGGGASVWEQTVVRE
EMWAHHEPRGAQYMGVNWVGPIIMRHGTEEQQRRHLPPIARGEVIWCQGFSEPEAGSDLASLRTTARRDGDGWLISGQKI
WTSYATMAQWCFLLARTSRGERKQQGQTIFLVPMDDPAIQVRPIRAMLGPHHLNEVFFDDLRVTEADVLGTVDAGWSIVQ
DVMAFERVGIARYARCERLLAAAPTVLGDRWGDLSEELRGRWVRMLTHCRRARLMAYRVVSLQSSGRIQPGDAAAYRIAV
TKLDQDSAEVLMDISAEVSRDDPRAAWFLGEVEDHWRYSQAATVSSGSIEMQRILLSRALLAAAK

Sequences:

>Translated_385_residues
MSMDFDMGPRAAELRSRLRELVSEHVPEHFLGAFTDDPADLETAQRFCRLLAEQDLLCLAWPQEFGGGGASVWEQTVVRE
EMWAHHEPRGAQYMGVNWVGPIIMRHGTEEQQRRHLPPIARGEVIWCQGFSEPEAGSDLASLRTTARRDGDGWLISGQKI
WTSYATMAQWCFLLARTSRGERKQQGQTIFLVPMDDPAIQVRPIRAMLGPHHLNEVFFDDLRVTEADVLGTVDAGWSIVQ
DVMAFERVGIARYARCERLLAAAPTVLGDRWGDLSEELRGRWVRMLTHCRRARLMAYRVVSLQSSGRIQPGDAAAYRIAV
TKLDQDSAEVLMDISAEVSRDDPRAAWFLGEVEDHWRYSQAATVSSGSIEMQRILLSRALLAAAK
>Mature_384_residues
SMDFDMGPRAAELRSRLRELVSEHVPEHFLGAFTDDPADLETAQRFCRLLAEQDLLCLAWPQEFGGGGASVWEQTVVREE
MWAHHEPRGAQYMGVNWVGPIIMRHGTEEQQRRHLPPIARGEVIWCQGFSEPEAGSDLASLRTTARRDGDGWLISGQKIW
TSYATMAQWCFLLARTSRGERKQQGQTIFLVPMDDPAIQVRPIRAMLGPHHLNEVFFDDLRVTEADVLGTVDAGWSIVQD
VMAFERVGIARYARCERLLAAAPTVLGDRWGDLSEELRGRWVRMLTHCRRARLMAYRVVSLQSSGRIQPGDAAAYRIAVT
KLDQDSAEVLMDISAEVSRDDPRAAWFLGEVEDHWRYSQAATVSSGSIEMQRILLSRALLAAAK

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=345, Percent_Identity=26.3768115942029, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI226958412, Length=400, Percent_Identity=22.75, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI226958414, Length=368, Percent_Identity=23.6413043478261, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4501859, Length=387, Percent_Identity=24.031007751938, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI4501857, Length=207, Percent_Identity=28.5024154589372, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI4557231, Length=216, Percent_Identity=28.7037037037037, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI187960098, Length=216, Percent_Identity=28.7037037037037, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI4557235, Length=250, Percent_Identity=30, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI76496475, Length=250, Percent_Identity=30, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI211971008, Length=166, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI48976061, Length=166, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1786223, Length=392, Percent_Identity=26.0204081632653, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081958, Length=264, Percent_Identity=28.030303030303, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17538396, Length=346, Percent_Identity=25.4335260115607, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17508101, Length=203, Percent_Identity=33.0049261083744, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17506239, Length=203, Percent_Identity=31.5270935960591, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32563615, Length=195, Percent_Identity=31.7948717948718, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17569725, Length=341, Percent_Identity=24.9266862170088, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17570075, Length=394, Percent_Identity=23.3502538071066, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17534899, Length=341, Percent_Identity=24.3401759530792, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71990804, Length=207, Percent_Identity=28.5024154589372, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17505929, Length=194, Percent_Identity=27.319587628866, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17551932, Length=219, Percent_Identity=25.1141552511416, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71985184, Length=211, Percent_Identity=26.5402843601896, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71985192, Length=211, Percent_Identity=26.5402843601896, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71982178, Length=200, Percent_Identity=26.5, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24666513, Length=348, Percent_Identity=26.4367816091954, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24646207, Length=349, Percent_Identity=26.0744985673352, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355753, Length=338, Percent_Identity=26.0355029585799, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356377, Length=346, Percent_Identity=23.6994219653179, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24660351, Length=304, Percent_Identity=23.6842105263158, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI281363737, Length=158, Percent_Identity=29.1139240506329, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 43402; Mature: 43271

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMDFDMGPRAAELRSRLRELVSEHVPEHFLGAFTDDPADLETAQRFCRLLAEQDLLCLA
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEE
WPQEFGGGGASVWEQTVVREEMWAHHEPRGAQYMGVNWVGPIIMRHGTEEQQRRHLPPIA
CCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCCHHHHHCCCCCC
RGEVIWCQGFSEPEAGSDLASLRTTARRDGDGWLISGQKIWTSYATMAQWCFLLARTSRG
CCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCC
ERKQQGQTIFLVPMDDPAIQVRPIRAMLGPHHLNEVFFDDLRVTEADVLGTVDAGWSIVQ
CHHHCCCEEEEEECCCCCEEEEHHHHHHCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHH
DVMAFERVGIARYARCERLLAAAPTVLGDRWGDLSEELRGRWVRMLTHCRRARLMAYRVV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLQSSGRIQPGDAAAYRIAVTKLDQDSAEVLMDISAEVSRDDPRAAWFLGEVEDHWRYSQ
EECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHH
AATVSSGSIEMQRILLSRALLAAAK
HCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SMDFDMGPRAAELRSRLRELVSEHVPEHFLGAFTDDPADLETAQRFCRLLAEQDLLCLA
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEE
WPQEFGGGGASVWEQTVVREEMWAHHEPRGAQYMGVNWVGPIIMRHGTEEQQRRHLPPIA
CCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCCHHHHHCCCCCC
RGEVIWCQGFSEPEAGSDLASLRTTARRDGDGWLISGQKIWTSYATMAQWCFLLARTSRG
CCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCC
ERKQQGQTIFLVPMDDPAIQVRPIRAMLGPHHLNEVFFDDLRVTEADVLGTVDAGWSIVQ
CHHHCCCEEEEEECCCCCEEEEHHHHHHCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHH
DVMAFERVGIARYARCERLLAAAPTVLGDRWGDLSEELRGRWVRMLTHCRRARLMAYRVV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLQSSGRIQPGDAAAYRIAVTKLDQDSAEVLMDISAEVSRDDPRAAWFLGEVEDHWRYSQ
EECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHH
AATVSSGSIEMQRILLSRALLAAAK
HCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]