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The map label for this gene is paaF [C]

Identifier: 108801362

GI number: 108801362

Start: 4677955

End: 4678731

Strand: Reverse

Name: paaF [C]

Synonym: Mmcs_4399

Alternate gene names: 108801362

Gene position: 4678731-4677955 (Counterclockwise)

Preceding gene: 108801363

Following gene: 108801361

Centisome position: 82.0

GC content: 70.53

Gene sequence:

>777_bases
ATGAAATACGACCTGCCCGACGAACTGACCGTCACCGCCGACGGGGCGGTGCGCACCGTGCTGATCAACCGGCCCGACGA
CCTGAACTGCGTCAACGAGAACCTGCACTGGGCGCTGGCCAACGTCTGGCGGCAACTGGCCGCCGACCAGGAGGCCCGCG
TGGTGATCCTCTCGGGTGCCGGGCGGGCGTTCTGCGCCGGCGGTGACCTCAACTGGATCACATCGTTCCTCGACGACTCC
GCCGCCCGCGACGAGAGCATCCGCGAGGGCGCCCAGATCATCGAGGAGATGCTGCGGTTCCCGCTTCCGGTGATCGCCGC
CGTCAACGGCCCCGCGGTCGGCCTGGGCTGCAGTGTCGCCCTGCTCAGCGACATCATGCTGATCGCCGAGGACGCCTACC
TGTCCGATCCGCACGTCGCCGTCGGTCTCGTGGCCGGCGACGGCGGGGCGGCGTTCTGGCCCATGCTCACCCCGATCATG
CGGTCACGCGAATACCTGTTCACCGGCGACCGGATCCCCGCCGCGACGGCCGTCGAACTCGGCTTCGCGAGCCGCCGGGT
TCCGGCGGCCGATCTGATGAGCGAGGCCGCCACACTCGCCGGACGGCTCGCGGCCCAACCCGTCGAGGCCCTGCAGGGCA
CCAAACGGGTGGTGAACATGTACCTGTCGCAGGCCATGGCCGGACCGCTGCAGGCCGGGTTCGCCGCCGAGGTCGCCACC
ATGCGCTCCGAGGACCACCGGCGCAGGCTGCTCGCGTTCCGGGAGGGTGCGACGTGA

Upstream 100 bases:

>100_bases
TGCGGACGGGCAGCCGGACCACCCCCTCGTTCGACGACGGACTCGCCGTCGCGCGCGTGATGGACCGATTGCGCGCCAAC
GCCGTTCGGATGGGCACCCG

Downstream 100 bases:

>100_bases
GCATCGACGACTTCCGCGCCACCGTGCGGGCGTGGTGTGCCGAACACGTGCCGTCGGACTGGCGCGAGAAGCAGACCGGC
GTGGGCGACGAGGAGTTCGT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKYDLPDELTVTADGAVRTVLINRPDDLNCVNENLHWALANVWRQLAADQEARVVILSGAGRAFCAGGDLNWITSFLDDS
AARDESIREGAQIIEEMLRFPLPVIAAVNGPAVGLGCSVALLSDIMLIAEDAYLSDPHVAVGLVAGDGGAAFWPMLTPIM
RSREYLFTGDRIPAATAVELGFASRRVPAADLMSEAATLAGRLAAQPVEALQGTKRVVNMYLSQAMAGPLQAGFAAEVAT
MRSEDHRRRLLAFREGAT

Sequences:

>Translated_258_residues
MKYDLPDELTVTADGAVRTVLINRPDDLNCVNENLHWALANVWRQLAADQEARVVILSGAGRAFCAGGDLNWITSFLDDS
AARDESIREGAQIIEEMLRFPLPVIAAVNGPAVGLGCSVALLSDIMLIAEDAYLSDPHVAVGLVAGDGGAAFWPMLTPIM
RSREYLFTGDRIPAATAVELGFASRRVPAADLMSEAATLAGRLAAQPVEALQGTKRVVNMYLSQAMAGPLQAGFAAEVAT
MRSEDHRRRLLAFREGAT
>Mature_258_residues
MKYDLPDELTVTADGAVRTVLINRPDDLNCVNENLHWALANVWRQLAADQEARVVILSGAGRAFCAGGDLNWITSFLDDS
AARDESIREGAQIIEEMLRFPLPVIAAVNGPAVGLGCSVALLSDIMLIAEDAYLSDPHVAVGLVAGDGGAAFWPMLTPIM
RSREYLFTGDRIPAATAVELGFASRRVPAADLMSEAATLAGRLAAQPVEALQGTKRVVNMYLSQAMAGPLQAGFAAEVAT
MRSEDHRRRLLAFREGAT

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=242, Percent_Identity=25.6198347107438, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI45643119, Length=217, Percent_Identity=24.8847926267281, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI260275230, Length=217, Percent_Identity=24.8847926267281, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4757968, Length=207, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI49355787, Length=207, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI260274832, Length=217, Percent_Identity=24.8847926267281, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI116235487, Length=218, Percent_Identity=25.6880733944954, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI25453481, Length=174, Percent_Identity=27.0114942528736, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI51538573, Length=174, Percent_Identity=27.0114942528736, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4757966, Length=174, Percent_Identity=27.0114942528736, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI51538581, Length=174, Percent_Identity=27.0114942528736, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI31542718, Length=203, Percent_Identity=29.5566502463054, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI221136756, Length=232, Percent_Identity=26.2931034482759, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI221136753, Length=232, Percent_Identity=26.2931034482759, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI221307494, Length=232, Percent_Identity=26.2931034482759, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI70995211, Length=227, Percent_Identity=32.15859030837, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=27.683615819209, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI68989263, Length=196, Percent_Identity=27.5510204081633, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=231, Percent_Identity=32.034632034632, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=28.125, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787660, Length=244, Percent_Identity=31.5573770491803, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=29.3193717277487, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788597, Length=212, Percent_Identity=26.4150943396226, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=29.0697674418605, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=29.6442687747036, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=220, Percent_Identity=24.5454545454545, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=214, Percent_Identity=28.5046728971963, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=196, Percent_Identity=27.0408163265306, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=223, Percent_Identity=28.6995515695067, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=27.9569892473118, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=178, Percent_Identity=28.6516853932584, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=193, Percent_Identity=29.5336787564767, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=193, Percent_Identity=29.5336787564767, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=29.6296296296296, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=29.6296296296296, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24654903, Length=218, Percent_Identity=26.1467889908257, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920382, Length=212, Percent_Identity=28.3018867924528, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21357171, Length=206, Percent_Identity=25.7281553398058, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653139, Length=229, Percent_Identity=27.5109170305677, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922422, Length=201, Percent_Identity=24.3781094527363, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27607; Mature: 27607

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDLPDELTVTADGAVRTVLINRPDDLNCVNENLHWALANVWRQLAADQEARVVILSGA
CCCCCCCCEEEEECCCEEEEEEECCCCCCHHCCCHHHHHHHHHHHHHCCCCCEEEEEECC
GRAFCAGGDLNWITSFLDDSAARDESIREGAQIIEEMLRFPLPVIAAVNGPAVGLGCSVA
CCEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
LLSDIMLIAEDAYLSDPHVAVGLVAGDGGAAFWPMLTPIMRSREYLFTGDRIPAATAVEL
HHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHH
GFASRRVPAADLMSEAATLAGRLAAQPVEALQGTKRVVNMYLSQAMAGPLQAGFAAEVAT
CHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHH
MRSEDHRRRLLAFREGAT
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKYDLPDELTVTADGAVRTVLINRPDDLNCVNENLHWALANVWRQLAADQEARVVILSGA
CCCCCCCCEEEEECCCEEEEEEECCCCCCHHCCCHHHHHHHHHHHHHCCCCCEEEEEECC
GRAFCAGGDLNWITSFLDDSAARDESIREGAQIIEEMLRFPLPVIAAVNGPAVGLGCSVA
CCEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
LLSDIMLIAEDAYLSDPHVAVGLVAGDGGAAFWPMLTPIMRSREYLFTGDRIPAATAVEL
HHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHH
GFASRRVPAADLMSEAATLAGRLAAQPVEALQGTKRVVNMYLSQAMAGPLQAGFAAEVAT
CHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHH
MRSEDHRRRLLAFREGAT
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA