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The map label for this gene is purR [H]

Identifier: 108801351

GI number: 108801351

Start: 4664300

End: 4665289

Strand: Reverse

Name: purR [H]

Synonym: Mmcs_4388

Alternate gene names: 108801351

Gene position: 4665289-4664300 (Counterclockwise)

Preceding gene: 108801352

Following gene: 108801347

Centisome position: 81.77

GC content: 72.12

Gene sequence:

>990_bases
ATGCGGGAGGTGGCCGCCGCGGCGGCCGTCTCCGTCGGCACGGTGTCCAACGTGCTCAACTCACCCGACAAGGTGGCCCC
GGCCACCGTCGCCCGCGTGCACGCGGCCATCGAGGAACTGGGGTTCGTCCGTAACGACGCCGCGCGCCAACTCAAGGCCG
GCCGGTCGCGCAGCGTCGGGCTGGTGGTGCTCGACGTCGGCAACCCGTTCTTCACCGACATCGCCCGCGCCGCCGAACAT
CGCGCGGGCGAACACAACCTCACCGTGCTGCTCGGTACGTCCGACGACGACCCCCGCCGCGAACGCGCCTACCTCGACGC
CTTCGACGAACAACGGGTCTTCGGACTTCTGGTGTCGCCGATCGGCGACGACCTCAGCCGGTTGCGCGCCTCCCAGCAGC
GGGGGACGCCGGTGGTGCTGGTCGACCGCGACGGCAGCGGCACACCGTTCGATTCGGTGGCGGTCGACGACGTCGCGGGC
GCCCGGTTGGCCGTCGATCACCTGTGCGCCACCGGACGTCGGCGGATCGCGTTCGTCAGCGGGCCGACCGAACTGCGGCA
GGTGCGCGACCGGCTTCGCGGTGCGCGCGAGGCGGTCGCCGAGGTTGCGGGTGCCACCCTCGAGGTGATCGAGACCCCGG
CGCTGACCGTGCTGGAGGGCCGCGCCGCGGGGGAACGGCTGCGGGAACGGCCGCCCGGTCGCCGCCCCGACGCAGTGTTC
TGCGCCAACGATCTACTGGCCATCGGCGTGCTGCAGGCGTTGGCGCTGATCGGGGACATCCGGGTACCCGACGACATCGC
GCTGGTCGGCTACGACGACATCGATTTCGCGCGTTCGGCCGTCGTGCCGCTGACGTCGGTGCGTCAGCCCACCCGGGCGA
TCGGGACGACGGCGATCGACCTGCTGATGGCCGCCGCCGACGCGGGCGGTGCGCACCGCCCCGACCACATCGTGTTCCAG
CCCGAACTCGTGACGCGCGACTCGACCTAA

Upstream 100 bases:

>100_bases
GACTCGTCGACGGGGGCCTCGACGCCCTGCGCACGCTGGTCGCTGACCGGTTCCCCGCGACCCGCTACGTCCCGTCTGGT
GCAATGGTGTCGTGGTGAGT

Downstream 100 bases:

>100_bases
CCGTCGACGATCAGCACGTCCAGCAGGCGGGGACCGTGCACACCCTCGACCCGGTTGAGCTCGATGTCGCTGGTGGCGCT
CGGACCGGAGATGAACGTCA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MREVAAAAAVSVGTVSNVLNSPDKVAPATVARVHAAIEELGFVRNDAARQLKAGRSRSVGLVVLDVGNPFFTDIARAAEH
RAGEHNLTVLLGTSDDDPRRERAYLDAFDEQRVFGLLVSPIGDDLSRLRASQQRGTPVVLVDRDGSGTPFDSVAVDDVAG
ARLAVDHLCATGRRRIAFVSGPTELRQVRDRLRGAREAVAEVAGATLEVIETPALTVLEGRAAGERLRERPPGRRPDAVF
CANDLLAIGVLQALALIGDIRVPDDIALVGYDDIDFARSAVVPLTSVRQPTRAIGTTAIDLLMAAADAGGAHRPDHIVFQ
PELVTRDST

Sequences:

>Translated_329_residues
MREVAAAAAVSVGTVSNVLNSPDKVAPATVARVHAAIEELGFVRNDAARQLKAGRSRSVGLVVLDVGNPFFTDIARAAEH
RAGEHNLTVLLGTSDDDPRRERAYLDAFDEQRVFGLLVSPIGDDLSRLRASQQRGTPVVLVDRDGSGTPFDSVAVDDVAG
ARLAVDHLCATGRRRIAFVSGPTELRQVRDRLRGAREAVAEVAGATLEVIETPALTVLEGRAAGERLRERPPGRRPDAVF
CANDLLAIGVLQALALIGDIRVPDDIALVGYDDIDFARSAVVPLTSVRQPTRAIGTTAIDLLMAAADAGGAHRPDHIVFQ
PELVTRDST
>Mature_329_residues
MREVAAAAAVSVGTVSNVLNSPDKVAPATVARVHAAIEELGFVRNDAARQLKAGRSRSVGLVVLDVGNPFFTDIARAAEH
RAGEHNLTVLLGTSDDDPRRERAYLDAFDEQRVFGLLVSPIGDDLSRLRASQQRGTPVVLVDRDGSGTPFDSVAVDDVAG
ARLAVDHLCATGRRRIAFVSGPTELRQVRDRLRGAREAVAEVAGATLEVIETPALTVLEGRAAGERLRERPPGRRPDAVF
CANDLLAIGVLQALALIGDIRVPDDIALVGYDDIDFARSAVVPLTSVRQPTRAIGTTAIDLLMAAADAGGAHRPDHIVFQ
PELVTRDST

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=31.858407079646, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=33.6569579288026, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=31.3609467455621, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789202, Length=341, Percent_Identity=33.1378299120235, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=31.9402985074627, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786540, Length=313, Percent_Identity=28.4345047923323, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787906, Length=333, Percent_Identity=28.8288288288288, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=29.6407185628743, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI48994940, Length=307, Percent_Identity=26.7100977198697, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787580, Length=311, Percent_Identity=24.4372990353698, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=25.08038585209, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786268, Length=323, Percent_Identity=27.2445820433437, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789456, Length=328, Percent_Identity=25.9146341463415, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790192, Length=224, Percent_Identity=26.7857142857143, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34974; Mature: 34974

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVAAAAAVSVGTVSNVLNSPDKVAPATVARVHAAIEELGFVRNDAARQLKAGRSRSVG
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LVVLDVGNPFFTDIARAAEHRAGEHNLTVLLGTSDDDPRRERAYLDAFDEQRVFGLLVSP
EEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHH
IGDDLSRLRASQQRGTPVVLVDRDGSGTPFDSVAVDDVAGARLAVDHLCATGRRRIAFVS
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEEC
GPTELRQVRDRLRGAREAVAEVAGATLEVIETPALTVLEGRAAGERLRERPPGRRPDAVF
CCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHCCCCCCCCEEE
CANDLLAIGVLQALALIGDIRVPDDIALVGYDDIDFARSAVVPLTSVRQPTRAIGTTAID
EHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCHHHCCCHHHHHHHHHHH
LLMAAADAGGAHRPDHIVFQPELVTRDST
HHHHHHCCCCCCCCCEEEECCCEECCCCC
>Mature Secondary Structure
MREVAAAAAVSVGTVSNVLNSPDKVAPATVARVHAAIEELGFVRNDAARQLKAGRSRSVG
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LVVLDVGNPFFTDIARAAEHRAGEHNLTVLLGTSDDDPRRERAYLDAFDEQRVFGLLVSP
EEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHH
IGDDLSRLRASQQRGTPVVLVDRDGSGTPFDSVAVDDVAGARLAVDHLCATGRRRIAFVS
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEEC
GPTELRQVRDRLRGAREAVAEVAGATLEVIETPALTVLEGRAAGERLRERPPGRRPDAVF
CCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHCCCCCCCCEEE
CANDLLAIGVLQALALIGDIRVPDDIALVGYDDIDFARSAVVPLTSVRQPTRAIGTTAID
EHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCHHHCCCHHHHHHHHHHH
LLMAAADAGGAHRPDHIVFQPELVTRDST
HHHHHHCCCCCCCCCEEEECCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA