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The map label for this gene is pcrA [H]

Identifier: 108801294

GI number: 108801294

Start: 4603985

End: 4606342

Strand: Reverse

Name: pcrA [H]

Synonym: Mmcs_4330

Alternate gene names: 108801294

Gene position: 4606342-4603985 (Counterclockwise)

Preceding gene: 108801296

Following gene: 108801291

Centisome position: 80.74

GC content: 67.43

Gene sequence:

>2358_bases
ATGAGTGTGAACGTGACTGAACCGAGTTCCCGGCCTGACGACAGGGCCGAGGAACTCCTCGACGGCCTCAACCCGCAGCA
GCGCCAGGCGGTGCTGCACGAGGGGTCGCCGCTGCTGATCGTGGCGGGAGCGGGGTCGGGTAAGACCGCGGTGCTGACCC
GCCGAATCGCCTACCTGCTGGCCGCCCGCGACGTCGGGGTCGGGCAGGTGCTGGCCATCACGTTCACCAACAAGGCCGCC
GCCGAGATGCGCGAACGCGTCGTCCATTTGGTGGGCCCGCGCGCGAGGTCGATGTGGGTGGCGACGTTCCACTCGACGTG
TGTGCGCATCCTGCGCAACCAGGCGTCGCTGCTGCCCGGGCTGAACTCCAACTTCTCGATCTACGACGCCGACGATTCGC
GGCGGCTGCTGTTGATGATCGGCAAGGACATGGGCCTGGACACCAAGAAGTACTCGCCCCGCCTGCTGTCCAACGGGATC
TCGAACCTGAAGAACGAGCTGATCGGGCCCGAACAGGCCGCCGCCGAGGCGTCGGAGGCCGCCGACGACCTGGCCCGCAT
CATCGCCGACGTCTACGCCGAGTACCAGCGCCGGCTGCGCGCCGCCAACGCGCTGGACTTCGACGACCTGATCGGCGAGA
CGGTCGCTGTGCTGCAACGTTTTCCGCAGATCGCGCAGTACTACCGCCGGCGCTTCCGGCACATCCTGGTCGACGAATAC
CAGGACACCAACCACGCCCAGTACGTGCTGGTGCGCGAGCTCGTAGGGGGCGGCGCCCACGACACCGGAGAGGTGGCACC
CGCGGAGCTCTGCGTGGTGGGCGACGCCGACCAGTCGATCTACGCGTTCCGCGGCGCGACGATCCGCAACATCGAGGACT
TCGAACGCGACTATCCCAACGCGACGACCATCCTGCTCGAGCAGAACTACCGCTCCACCCAGACCATCCTCAACGCCGCC
AACTCGGTGATCGCCCGCAACGCCGGCCGGCGCGAGAAGCGGCTGTGGACGGACTCCGGCCACGGGGAGCTGATCGTCGG
CTACGTCGCCGACAACGAACACGACGAGGCCCGGTTCGTCGCGCAGGAGATCGACCGGCTGGCCGATGACGGCCACAGCT
ACAACGATTTCGCCGTCTTCTACCGGACCAACAACAACTCCCGTGCGTTGGAAGAGGTGTTCATCCGCGCCGGCATCCCG
TACAAGGTCGTCGGCGGCGTCCGGTTCTACGAACGCCGCGAGATCCGCGACATCGTCGCCTACCTGCGGGTACTCGACAA
CCCCGGCGACTCGGTGAGCATGCGCCGCATCCTCAACACCCCGCGGCGCGGGATCGGCGACCGGGCCGAGGCGTGTGTGG
CGGTGTACGCCGAGAACACCGGTGCCAGCTTCAACGACGCGCTGCAGGCCGCCGCCGAGGGCAAGGTGCCGATGCTCAAC
ACCCGCTCGGAGAAGTGCATCGCGAGTTTCGTGCAGATGCTCGACGATCTGCGCGGCCGCCTCGGTGACGAACTCGGCGA
ACTCGTCGAGGCGGTGCTCGACCGCACCGGCTACCGCGCGGAGCTCGAGGCGTCCAATGATCCGCAGGACCTCGCGCGGC
TGGACAACCTCAACGAATTGGTCAGCGTCGCACACGAGTTCAGCATCGACCGGGCGAACGCCGCGGCGCTGGCCGAAGAC
CTCGACGAACCGGTCGACGAGGACATCCCCGATACCGGTGTGCTGGCGCAGTTCCTCGAGCGGGTGTCGCTGGTGGCCGA
CGCCGACGACATCCCCGAGCACGGCTCGGGCGTCGTCACCATGATGACGCTGCACACCGCCAAGGGACTGGAGTTCCCCG
TCGTGTTCGTCACCGGCTGGGAGGACGGCATGTTCCCGCACATGCGGGCACTCGGCGATCCCAACGAACTGTCCGAGGAA
CGCCGGTTGGCCTACGTCGGCATCACCCGGGCCCGTCAGCGGCTCTATCTCAGCCGGGCGAAGGTGCGCTCGTCGTGGGG
GCAACCCATGCTCAACCCGGAGTCGCGGTTCCTGCGGGAGATCCCGCAGGAGCTCATCGACTGGCGGCGCACCGATCCCG
AGAACAGAAGGCCGTCGTTCAGCGCGCCCAGCCGGATGTCGTCGGGCTCCTCCGGTACGAGTTTCGGCGCACCGCGCCCG
TCGCCGATGCGCTCGGGTGGCGGTAAGAAGCCGCTGATCGTGCTCGAACCGGGTGACCGCATCAACCACGACAAGTACGG
TCTGGGCCGCGTCGAAGAGGTCTCGGGCGTCGGCGAATCCGCGATGTCACTGATCGACTTCGGCAGCGCCGGACGCGTCA
AGCTGATGCACAACCACGCTCCGGTGCAGAAGCTCTAA

Upstream 100 bases:

>100_bases
CGTAAAAAAATCGCCACTGGTCAAGCACGGTTGTCAGTGTGCCACCTGCGGCCGTGCCAGCGCAAAGAGTGTCACCGTGC
GGCGGTAAGTTGGGACAGAC

Downstream 100 bases:

>100_bases
CCGGATTGGGCCCACCGGCCCGTGCTGGGCAGCAGCGTCAGCGCGATCGCCGCCACCGGCAGCACCGGGATTGCGTAGAC
GATCCACGGGAATTTCGCGC

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 785; Mature: 784

Protein sequence:

>785_residues
MSVNVTEPSSRPDDRAEELLDGLNPQQRQAVLHEGSPLLIVAGAGSGKTAVLTRRIAYLLAARDVGVGQVLAITFTNKAA
AEMRERVVHLVGPRARSMWVATFHSTCVRILRNQASLLPGLNSNFSIYDADDSRRLLLMIGKDMGLDTKKYSPRLLSNGI
SNLKNELIGPEQAAAEASEAADDLARIIADVYAEYQRRLRAANALDFDDLIGETVAVLQRFPQIAQYYRRRFRHILVDEY
QDTNHAQYVLVRELVGGGAHDTGEVAPAELCVVGDADQSIYAFRGATIRNIEDFERDYPNATTILLEQNYRSTQTILNAA
NSVIARNAGRREKRLWTDSGHGELIVGYVADNEHDEARFVAQEIDRLADDGHSYNDFAVFYRTNNNSRALEEVFIRAGIP
YKVVGGVRFYERREIRDIVAYLRVLDNPGDSVSMRRILNTPRRGIGDRAEACVAVYAENTGASFNDALQAAAEGKVPMLN
TRSEKCIASFVQMLDDLRGRLGDELGELVEAVLDRTGYRAELEASNDPQDLARLDNLNELVSVAHEFSIDRANAAALAED
LDEPVDEDIPDTGVLAQFLERVSLVADADDIPEHGSGVVTMMTLHTAKGLEFPVVFVTGWEDGMFPHMRALGDPNELSEE
RRLAYVGITRARQRLYLSRAKVRSSWGQPMLNPESRFLREIPQELIDWRRTDPENRRPSFSAPSRMSSGSSGTSFGAPRP
SPMRSGGGKKPLIVLEPGDRINHDKYGLGRVEEVSGVGESAMSLIDFGSAGRVKLMHNHAPVQKL

Sequences:

>Translated_785_residues
MSVNVTEPSSRPDDRAEELLDGLNPQQRQAVLHEGSPLLIVAGAGSGKTAVLTRRIAYLLAARDVGVGQVLAITFTNKAA
AEMRERVVHLVGPRARSMWVATFHSTCVRILRNQASLLPGLNSNFSIYDADDSRRLLLMIGKDMGLDTKKYSPRLLSNGI
SNLKNELIGPEQAAAEASEAADDLARIIADVYAEYQRRLRAANALDFDDLIGETVAVLQRFPQIAQYYRRRFRHILVDEY
QDTNHAQYVLVRELVGGGAHDTGEVAPAELCVVGDADQSIYAFRGATIRNIEDFERDYPNATTILLEQNYRSTQTILNAA
NSVIARNAGRREKRLWTDSGHGELIVGYVADNEHDEARFVAQEIDRLADDGHSYNDFAVFYRTNNNSRALEEVFIRAGIP
YKVVGGVRFYERREIRDIVAYLRVLDNPGDSVSMRRILNTPRRGIGDRAEACVAVYAENTGASFNDALQAAAEGKVPMLN
TRSEKCIASFVQMLDDLRGRLGDELGELVEAVLDRTGYRAELEASNDPQDLARLDNLNELVSVAHEFSIDRANAAALAED
LDEPVDEDIPDTGVLAQFLERVSLVADADDIPEHGSGVVTMMTLHTAKGLEFPVVFVTGWEDGMFPHMRALGDPNELSEE
RRLAYVGITRARQRLYLSRAKVRSSWGQPMLNPESRFLREIPQELIDWRRTDPENRRPSFSAPSRMSSGSSGTSFGAPRP
SPMRSGGGKKPLIVLEPGDRINHDKYGLGRVEEVSGVGESAMSLIDFGSAGRVKLMHNHAPVQKL
>Mature_784_residues
SVNVTEPSSRPDDRAEELLDGLNPQQRQAVLHEGSPLLIVAGAGSGKTAVLTRRIAYLLAARDVGVGQVLAITFTNKAAA
EMRERVVHLVGPRARSMWVATFHSTCVRILRNQASLLPGLNSNFSIYDADDSRRLLLMIGKDMGLDTKKYSPRLLSNGIS
NLKNELIGPEQAAAEASEAADDLARIIADVYAEYQRRLRAANALDFDDLIGETVAVLQRFPQIAQYYRRRFRHILVDEYQ
DTNHAQYVLVRELVGGGAHDTGEVAPAELCVVGDADQSIYAFRGATIRNIEDFERDYPNATTILLEQNYRSTQTILNAAN
SVIARNAGRREKRLWTDSGHGELIVGYVADNEHDEARFVAQEIDRLADDGHSYNDFAVFYRTNNNSRALEEVFIRAGIPY
KVVGGVRFYERREIRDIVAYLRVLDNPGDSVSMRRILNTPRRGIGDRAEACVAVYAENTGASFNDALQAAAEGKVPMLNT
RSEKCIASFVQMLDDLRGRLGDELGELVEAVLDRTGYRAELEASNDPQDLARLDNLNELVSVAHEFSIDRANAAALAEDL
DEPVDEDIPDTGVLAQFLERVSLVADADDIPEHGSGVVTMMTLHTAKGLEFPVVFVTGWEDGMFPHMRALGDPNELSEER
RLAYVGITRARQRLYLSRAKVRSSWGQPMLNPESRFLREIPQELIDWRRTDPENRRPSFSAPSRMSSGSSGTSFGAPRPS
PMRSGGGKKPLIVLEPGDRINHDKYGLGRVEEVSGVGESAMSLIDFGSAGRVKLMHNHAPVQKL

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=768, Percent_Identity=38.1510416666667, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI48994965, Length=702, Percent_Identity=39.031339031339, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1787196, Length=348, Percent_Identity=27.8735632183908, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=763, Percent_Identity=28.8335517693316, Blast_Score=248, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6324477, Length=706, Percent_Identity=22.0963172804533, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 86914; Mature: 86783

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNVTEPSSRPDDRAEELLDGLNPQQRQAVLHEGSPLLIVAGAGSGKTAVLTRRIAYLL
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
AARDVGVGQVLAITFTNKAAAEMRERVVHLVGPRARSMWVATFHSTCVRILRNQASLLPG
HHCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
LNSNFSIYDADDSRRLLLMIGKDMGLDTKKYSPRLLSNGISNLKNELIGPEQAAAEASEA
CCCCCEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ADDLARIIADVYAEYQRRLRAANALDFDDLIGETVAVLQRFPQIAQYYRRRFRHILVDEY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDTNHAQYVLVRELVGGGAHDTGEVAPAELCVVGDADQSIYAFRGATIRNIEDFERDYPN
CCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCHHHHHHCCCC
ATTILLEQNYRSTQTILNAANSVIARNAGRREKRLWTDSGHGELIVGYVADNEHDEARFV
CEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHH
AQEIDRLADDGHSYNDFAVFYRTNNNSRALEEVFIRAGIPYKVVGGVRFYERREIRDIVA
HHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
YLRVLDNPGDSVSMRRILNTPRRGIGDRAEACVAVYAENTGASFNDALQAAAEGKVPMLN
HHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHCCCCCCCC
TRSEKCIASFVQMLDDLRGRLGDELGELVEAVLDRTGYRAELEASNDPQDLARLDNLNEL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCHHHH
VSVAHEFSIDRANAAALAEDLDEPVDEDIPDTGVLAQFLERVSLVADADDIPEHGSGVVT
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
MMTLHTAKGLEFPVVFVTGWEDGMFPHMRALGDPNELSEERRLAYVGITRARQRLYLSRA
EEEEHHCCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHHEEHHHHHHHHHHHHHHHH
KVRSSWGQPMLNPESRFLREIPQELIDWRRTDPENRRPSFSAPSRMSSGSSGTSFGAPRP
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SPMRSGGGKKPLIVLEPGDRINHDKYGLGRVEEVSGVGESAMSLIDFGSAGRVKLMHNHA
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCEEEEECCC
PVQKL
CCCCC
>Mature Secondary Structure 
SVNVTEPSSRPDDRAEELLDGLNPQQRQAVLHEGSPLLIVAGAGSGKTAVLTRRIAYLL
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
AARDVGVGQVLAITFTNKAAAEMRERVVHLVGPRARSMWVATFHSTCVRILRNQASLLPG
HHCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
LNSNFSIYDADDSRRLLLMIGKDMGLDTKKYSPRLLSNGISNLKNELIGPEQAAAEASEA
CCCCCEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ADDLARIIADVYAEYQRRLRAANALDFDDLIGETVAVLQRFPQIAQYYRRRFRHILVDEY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDTNHAQYVLVRELVGGGAHDTGEVAPAELCVVGDADQSIYAFRGATIRNIEDFERDYPN
CCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCHHHHHHCCCC
ATTILLEQNYRSTQTILNAANSVIARNAGRREKRLWTDSGHGELIVGYVADNEHDEARFV
CEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHH
AQEIDRLADDGHSYNDFAVFYRTNNNSRALEEVFIRAGIPYKVVGGVRFYERREIRDIVA
HHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
YLRVLDNPGDSVSMRRILNTPRRGIGDRAEACVAVYAENTGASFNDALQAAAEGKVPMLN
HHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHCCCCCCCC
TRSEKCIASFVQMLDDLRGRLGDELGELVEAVLDRTGYRAELEASNDPQDLARLDNLNEL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCHHHH
VSVAHEFSIDRANAAALAEDLDEPVDEDIPDTGVLAQFLERVSLVADADDIPEHGSGVVT
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
MMTLHTAKGLEFPVVFVTGWEDGMFPHMRALGDPNELSEERRLAYVGITRARQRLYLSRA
EEEEHHCCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHHEEHHHHHHHHHHHHHHHH
KVRSSWGQPMLNPESRFLREIPQELIDWRRTDPENRRPSFSAPSRMSSGSSGTSFGAPRP
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SPMRSGGGKKPLIVLEPGDRINHDKYGLGRVEEVSGVGESAMSLIDFGSAGRVKLMHNHA
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCEEEEECCC
PVQKL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]