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The map label for this gene is ydiB [C]

Identifier: 108801247

GI number: 108801247

Start: 4550731

End: 4551555

Strand: Reverse

Name: ydiB [C]

Synonym: Mmcs_4283

Alternate gene names: 108801247

Gene position: 4551555-4550731 (Counterclockwise)

Preceding gene: 108801248

Following gene: 108801245

Centisome position: 79.78

GC content: 68.97

Gene sequence:

>825_bases
ATGGGGCGGCCGCCGCTGAACAAGGACACCCGGCTGTGCATCTCGCTGGCCGGGCGTCCCAGCAACATCGGCACCCGGTT
CCACAACCACCTCTACGAGGTCCTCGGGTTGGACTTCCTCTACAAGGCGTTCACCACCACCGACATCGGCGCGGCCATCG
GCGGCGTACGGGCGCTGGGCATCCGCGGGTGTTCGGTGTCGATGCCGTTCAAACAGGACGTACTCGACCTCGTCGACGAG
GTCGAGCCGTCCGCACGAATCATCAACGCCGTCAATACGATTGTGAACGACGACGGGCATCTCACCGCGTCGAACACCGA
TTACCTGGCCGTGCAACGCCTCATCGACCAGTACGGCCTGGATTCCGGTTGCTCGGTGATGGTCCAGGGCAGCGGAGGCA
TGGCGTCCGCGGTCGGCGCGGCCTTCCGCGACCGCGGCTTCGCGACGGGGACGGTGGTGGCGCGCAATGAAACCACCGGG
CGCGCCCTGGCCGAACGCCTCGGCTACGACTACGCCGCCCAGGTCGCATCGCGGTCCGCCGAGGTGATCGTCAACGTCAC
GCCCATCGGGATGGCCGGCGGTCCCGAAGAGCGGCAGCAGGCGTTCGACGCCGACACGATCGCGCAGGCGCACACCGTAT
TCGACGTCGTCGCGACGCCCGCCGAGACGCCGCTGATCACCGCCGCGCGCCAGGCAGGGGTCGCGGTGATCACCGGTGCG
GAGGTGGTGGCATTGCAGGCCGCGGAGCAGTTCGAGCGCTACACCGGGGTGCGGCCGACGCCGGAGCAGGTCGCCGAGGC
GTCGGCGATATCCCGCCGGGACTGA

Upstream 100 bases:

>100_bases
CCACCGTCGATGTGCCCGACCCGGCCACGCTGCCGTTCCCGCCGGAACTCACCGGCCGGATCCGCGACGTCACCCGCCAG
GTCATCCGGGCCGTCGGCTG

Downstream 100 bases:

>100_bases
CCGGTCTCAGGGCGTGATCTTCGCCTGTTCGTCGATGGCCGCCGTGGCGTCCATCAGCGGCCCGATCTGATCCTCGCGAC
CGTCGGCATTGATCTGCAGC

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MGRPPLNKDTRLCISLAGRPSNIGTRFHNHLYEVLGLDFLYKAFTTTDIGAAIGGVRALGIRGCSVSMPFKQDVLDLVDE
VEPSARIINAVNTIVNDDGHLTASNTDYLAVQRLIDQYGLDSGCSVMVQGSGGMASAVGAAFRDRGFATGTVVARNETTG
RALAERLGYDYAAQVASRSAEVIVNVTPIGMAGGPEERQQAFDADTIAQAHTVFDVVATPAETPLITAARQAGVAVITGA
EVVALQAAEQFERYTGVRPTPEQVAEASAISRRD

Sequences:

>Translated_274_residues
MGRPPLNKDTRLCISLAGRPSNIGTRFHNHLYEVLGLDFLYKAFTTTDIGAAIGGVRALGIRGCSVSMPFKQDVLDLVDE
VEPSARIINAVNTIVNDDGHLTASNTDYLAVQRLIDQYGLDSGCSVMVQGSGGMASAVGAAFRDRGFATGTVVARNETTG
RALAERLGYDYAAQVASRSAEVIVNVTPIGMAGGPEERQQAFDADTIAQAHTVFDVVATPAETPLITAARQAGVAVITGA
EVVALQAAEQFERYTGVRPTPEQVAEASAISRRD
>Mature_273_residues
GRPPLNKDTRLCISLAGRPSNIGTRFHNHLYEVLGLDFLYKAFTTTDIGAAIGGVRALGIRGCSVSMPFKQDVLDLVDEV
EPSARIINAVNTIVNDDGHLTASNTDYLAVQRLIDQYGLDSGCSVMVQGSGGMASAVGAAFRDRGFATGTVVARNETTGR
ALAERLGYDYAAQVASRSAEVIVNVTPIGMAGGPEERQQAFDADTIAQAHTVFDVVATPAETPLITAARQAGVAVITGAE
VVALQAAEQFERYTGVRPTPEQVAEASAISRRD

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=266, Percent_Identity=33.4586466165414, Blast_Score=114, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6320332, Length=254, Percent_Identity=23.6220472440945, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 28913; Mature: 28782

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRPPLNKDTRLCISLAGRPSNIGTRFHNHLYEVLGLDFLYKAFTTTDIGAAIGGVRALG
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
IRGCSVSMPFKQDVLDLVDEVEPSARIINAVNTIVNDDGHLTASNTDYLAVQRLIDQYGL
ECCCEECCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCC
DSGCSVMVQGSGGMASAVGAAFRDRGFATGTVVARNETTGRALAERLGYDYAAQVASRSA
CCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHCCCC
EVIVNVTPIGMAGGPEERQQAFDADTIAQAHTVFDVVATPAETPLITAARQAGVAVITGA
EEEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEECC
EVVALQAAEQFERYTGVRPTPEQVAEASAISRRD
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
GRPPLNKDTRLCISLAGRPSNIGTRFHNHLYEVLGLDFLYKAFTTTDIGAAIGGVRALG
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
IRGCSVSMPFKQDVLDLVDEVEPSARIINAVNTIVNDDGHLTASNTDYLAVQRLIDQYGL
ECCCEECCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCC
DSGCSVMVQGSGGMASAVGAAFRDRGFATGTVVARNETTGRALAERLGYDYAAQVASRSA
CCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHCCCC
EVIVNVTPIGMAGGPEERQQAFDADTIAQAHTVFDVVATPAETPLITAARQAGVAVITGA
EEEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEECC
EVVALQAAEQFERYTGVRPTPEQVAEASAISRRD
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]