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The map label for this gene is metB [H]

Identifier: 108801110

GI number: 108801110

Start: 4411050

End: 4412228

Strand: Reverse

Name: metB [H]

Synonym: Mmcs_4146

Alternate gene names: 108801110

Gene position: 4412228-4411050 (Counterclockwise)

Preceding gene: 108801112

Following gene: 108801106

Centisome position: 77.33

GC content: 69.04

Gene sequence:

>1179_bases
ATGACTGAACAGCGCAGCGCCCACGGACCCGCCCACGGACTCGCCACTGGACTCGCCACGAAGGCCATCCACGCCGGGTA
CGCCCCCGACCCCCGCACCGGTGCGGTCAACACGCCGATCTACGCCAGCTCGACCTTCGCCCAGGACGGTGTCGGAGGGC
TGCGGGGCGGCTTCGAGTACGCCCGCACCGGCAACCCGACGCGGCAGTCCCTGGAGTCGGCGCTGGCCGCGGTGGAGGAA
GCCGGGTACGGCCGGGCCTTCGCCTCCGGGATGGCCGCCACCGACTGTGCGTTGCGCGCGGTGCTGCGCCCCGGTGACCA
TCTCGTCATCCCGGACGACGCCTACGGCGGCACGTTCCGGCTGATCGACAAGGTGTTCTCCCAGTGGGGCATCAGCTACA
CGGCGGTCGCGCTGTCGGACCTCGACGCCGTCCGGGCGGCGATCACCCCGCAGACCAAGATGATCTGGGTCGAGACGCCG
ACCAACCCGCTGCTGTCGATCGCCGACATCGCCGGTATCGCAGGCATCGCGGCCGAATCGGGGCTGAAGGTGTTGGTGGA
CAACACCTTTGCCTCGCCCGCGTTACAGCAGCCGTTGCTGCTCGGCGCGGACATCGTGTTGCACTCCACCACGAAGTACA
TCGGCGGACACTCCGACGTGGTCGGCGGCGCGCTGCTGACCAACGACGAGGAACTCGACACGGCGTTCGCCTTCCTGCAG
AACGGATCGGGCGCCGTCCCAGGACCGTTCGACGCCTACCTCACCCTGCGCGGACTCAAGACGCTGGTGGTGCGGATGCA
GCGACACAGCGAGAACGCTGCGGCGGTCGCCGAATTCCTCGACGGCCACCCGGCGATCGACACGGTGCTCTATCCCGGTC
TGCCCAGCCATCCCGGCCACGACGTGGCCGCCAAGCAGATGAGCGGGTTCGGCGGCATGGTGTCGGTGCGGCTACACGGC
GGGCCCAAGGCGGCTCGCGAATTCTGCGCCCGCACAGGCATTTTCATCCTCGCCGAATCGCTGGGCGGGGTGGAGTCGCT
GATCGAGCATCCGGGTGCGATGACGCACGCGTCCACCGCCGGATCCCAACTCGAGGTGCCCGAGGATCTGGTGCGGCTGT
CGGTCGGCATCGAGGACCGCGCGGATCTGATCGCCGACCTGGAGCAGGCGCTAGCCTAA

Upstream 100 bases:

>100_bases
GTTCCTCGGGGGCGAGCATGGCGTCGGTGTCCAGCAGATCGTCGATACGTACCGTCATGAACCCATGTCTACACGGCGCT
TCGATAGGCTCGACGGTGTT

Downstream 100 bases:

>100_bases
CGCCGCGCGCAGCGCCGCCGCGGTGACCGCGAGGTTCACCTCGGCCAGCCCGGTCGGCGACTCGTCGACCCAACTGCCGA
GCGAGATCTCACCGGCCCGG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTEQRSAHGPAHGLATGLATKAIHAGYAPDPRTGAVNTPIYASSTFAQDGVGGLRGGFEYARTGNPTRQSLESALAAVEE
AGYGRAFASGMAATDCALRAVLRPGDHLVIPDDAYGGTFRLIDKVFSQWGISYTAVALSDLDAVRAAITPQTKMIWVETP
TNPLLSIADIAGIAGIAAESGLKVLVDNTFASPALQQPLLLGADIVLHSTTKYIGGHSDVVGGALLTNDEELDTAFAFLQ
NGSGAVPGPFDAYLTLRGLKTLVVRMQRHSENAAAVAEFLDGHPAIDTVLYPGLPSHPGHDVAAKQMSGFGGMVSVRLHG
GPKAAREFCARTGIFILAESLGGVESLIEHPGAMTHASTAGSQLEVPEDLVRLSVGIEDRADLIADLEQALA

Sequences:

>Translated_392_residues
MTEQRSAHGPAHGLATGLATKAIHAGYAPDPRTGAVNTPIYASSTFAQDGVGGLRGGFEYARTGNPTRQSLESALAAVEE
AGYGRAFASGMAATDCALRAVLRPGDHLVIPDDAYGGTFRLIDKVFSQWGISYTAVALSDLDAVRAAITPQTKMIWVETP
TNPLLSIADIAGIAGIAAESGLKVLVDNTFASPALQQPLLLGADIVLHSTTKYIGGHSDVVGGALLTNDEELDTAFAFLQ
NGSGAVPGPFDAYLTLRGLKTLVVRMQRHSENAAAVAEFLDGHPAIDTVLYPGLPSHPGHDVAAKQMSGFGGMVSVRLHG
GPKAAREFCARTGIFILAESLGGVESLIEHPGAMTHASTAGSQLEVPEDLVRLSVGIEDRADLIADLEQALA
>Mature_391_residues
TEQRSAHGPAHGLATGLATKAIHAGYAPDPRTGAVNTPIYASSTFAQDGVGGLRGGFEYARTGNPTRQSLESALAAVEEA
GYGRAFASGMAATDCALRAVLRPGDHLVIPDDAYGGTFRLIDKVFSQWGISYTAVALSDLDAVRAAITPQTKMIWVETPT
NPLLSIADIAGIAGIAAESGLKVLVDNTFASPALQQPLLLGADIVLHSTTKYIGGHSDVVGGALLTNDEELDTAFAFLQN
GSGAVPGPFDAYLTLRGLKTLVVRMQRHSENAAAVAEFLDGHPAIDTVLYPGLPSHPGHDVAAKQMSGFGGMVSVRLHGG
PKAAREFCARTGIFILAESLGGVESLIEHPGAMTHASTAGSQLEVPEDLVRLSVGIEDRADLIADLEQALA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=379, Percent_Identity=49.6042216358839, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI299473759, Length=379, Percent_Identity=45.3825857519789, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI25453487, Length=378, Percent_Identity=43.6507936507937, Blast_Score=269, Evalue=4e-72,
Organism=Escherichia coli, GI1790375, Length=374, Percent_Identity=44.9197860962567, Blast_Score=307, Evalue=7e-85,
Organism=Escherichia coli, GI1789383, Length=398, Percent_Identity=30.4020100502513, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17560056, Length=379, Percent_Identity=48.0211081794195, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17538055, Length=379, Percent_Identity=48.8126649076517, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI71980708, Length=368, Percent_Identity=38.8586956521739, Blast_Score=249, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6319307, Length=386, Percent_Identity=48.1865284974093, Blast_Score=317, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6321254, Length=401, Percent_Identity=31.9201995012469, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=419, Percent_Identity=30.7875894988067, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=307, Percent_Identity=29.9674267100977, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6321904, Length=391, Percent_Identity=29.4117647058824, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI19922554, Length=382, Percent_Identity=48.4293193717278, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 40711; Mature: 40580

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQRSAHGPAHGLATGLATKAIHAGYAPDPRTGAVNTPIYASSTFAQDGVGGLRGGFEY
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHCCCHH
ARTGNPTRQSLESALAAVEEAGYGRAFASGMAATDCALRAVLRPGDHLVIPDDAYGGTFR
HHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHH
LIDKVFSQWGISYTAVALSDLDAVRAAITPQTKMIWVETPTNPLLSIADIAGIAGIAAES
HHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCC
GLKVLVDNTFASPALQQPLLLGADIVLHSTTKYIGGHSDVVGGALLTNDEELDTAFAFLQ
CCEEEEECCCCCHHHHCCHHHCCCEEEECCHHHCCCCCHHCCCEEECCCHHHHHHHHHHH
NGSGAVPGPFDAYLTLRGLKTLVVRMQRHSENAAAVAEFLDGHPAIDTVLYPGLPSHPGH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHEECCCCCCCCCC
DVAAKQMSGFGGMVSVRLHGGPKAAREFCARTGIFILAESLGGVESLIEHPGAMTHASTA
CHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCHHEEEHHHCCHHHHHHCCCCCCCCCCC
GSQLEVPEDLVRLSVGIEDRADLIADLEQALA
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
TEQRSAHGPAHGLATGLATKAIHAGYAPDPRTGAVNTPIYASSTFAQDGVGGLRGGFEY
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHCCCHH
ARTGNPTRQSLESALAAVEEAGYGRAFASGMAATDCALRAVLRPGDHLVIPDDAYGGTFR
HHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHH
LIDKVFSQWGISYTAVALSDLDAVRAAITPQTKMIWVETPTNPLLSIADIAGIAGIAAES
HHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCC
GLKVLVDNTFASPALQQPLLLGADIVLHSTTKYIGGHSDVVGGALLTNDEELDTAFAFLQ
CCEEEEECCCCCHHHHCCHHHCCCEEEECCHHHCCCCCHHCCCEEECCCHHHHHHHHHHH
NGSGAVPGPFDAYLTLRGLKTLVVRMQRHSENAAAVAEFLDGHPAIDTVLYPGLPSHPGH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHEECCCCCCCCCC
DVAAKQMSGFGGMVSVRLHGGPKAAREFCARTGIFILAESLGGVESLIEHPGAMTHASTA
CHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCHHEEEHHHCCHHHHHHCCCCCCCCCCC
GSQLEVPEDLVRLSVGIEDRADLIADLEQALA
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]