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The map label for this gene is hsdR [H]

Identifier: 108800744

GI number: 108800744

Start: 4031427

End: 4033838

Strand: Reverse

Name: hsdR [H]

Synonym: Mmcs_3779

Alternate gene names: 108800744

Gene position: 4033838-4031427 (Counterclockwise)

Preceding gene: 108800745

Following gene: 108800743

Centisome position: 70.7

GC content: 64.18

Gene sequence:

>2412_bases
ATGAGTGCCTCGGATGACGACGCCCGGCTGCCGGCCGAAGCGCGCGCCCGCAAACTTATCGATGCACAGCTGACGCAGGC
CGGTTGGGTTGTCCAGGACAAGCAGGACCTCAACCTCTTCGCGGCGCAAGGCGTCGCCGTCCGCGAGGTGGTGATGAAGC
CCGGCCACGGCCGTGTCGATTACCTGTTGTACGTCGACAAAGCCGTGGTCGGCGTCATCGAGGCTAAACCGGTCGGCACG
CCGCTCTCCGGTGTGGAGTGGCAGTCGGCAATGTACGCCGACGGTCTGCCCGCCGACGTCAGGATTGCGGCCAAGACACG
CGATGGCAGGTTGCCGTTCGTGTTCGAGGCTTCCGGCGTCGAAACGCACTTCACCAACGGCTTCGAGCCGGAATCGCGGG
CGCGGCTGATCTTCAATGTCCCGCGACCGGAGACGCTCGCGCGTTATCTGCGCGAGGCCGAGACAAACCCCGATTACCCC
ACCTGGCGTGCGAAGGTCCGCAAGCTGCCGGCGCTGGACACGGCTGCTCTGCGGCCGGCGCAGATCGAGGCCGTCAACGG
GGTGGAGCAGAGCCTGGCCGGTCAGCAGTGCAACCGGTCGTTGGTGCAGATGGCGACCGGCGCGGGCAAGACGTTCACTG
CGGTGACGCAGTCCTACCGGCTGCTCAAGCACGGCGGCTTCGAACGGATCCTGTTCCTGGTCGACCGCAACAATCTGGCC
GACCAGACGTTGGGCGAATTTCAGAACTACCGCACACCCGATGACGGTCGACGTTTCACCGAGCTGTACAACGTCAACAA
GCTTTCCAGCGCGGGCTTGCTGGGATCGACCAAGGTCACGATCTCAACGATTCAGCGGGTGTTCCGTTTCATCAAGGCCG
GCGAGGTCAGTGATGCCGACGACCCCGACATCGACGATTACGTGCCGGACGCGCCTGTCACCGTTTCGTACAGCGAGGCG
CTGCCACCGGAGACCTTCGACCTGGTCATTGTCGACGAGGCGCACCGCAGCATCTACGGCGTGTGGCGCGGAGTCCTGGA
GTACTTCGATGCGCACGTCGTCGGGCTGACGGCGACACCGGGCAAGCAGACGTTCGCGTTCTTCCGGCAGAACCTGGTGT
CGGACTACACCTACCCGGAATCGGTGGCCGACGGCGTCAACGTCGACTTCGACGTCTACCGCATCCGCACCAAGATCAGC
GATCAGGGATCGCATATCGATGCCGGAACCATCGTCCCCAAGGTGGACCGCCGCACCCGCGAACAGCGTCTGGAAGCACT
CGATGACGATCTGGATTACGTTCCCGGGCAACTTGATCGAGCAGTCACCGCCACAGATCAGATCCGCACGGTGCTGGAGA
CTTTTCGCGACCGGCTGTTCACCGAGATCTTTCCCGGCCGCAGTACGGTCCCGAAGACGCTGATCTTCGCCAAGGACGAC
AACCACGCCGAGGAGATCGTCAGACAGGTGCGTGAGGTGTTCGGCAAGGGCAACGACTTTGCCGCCAAGATCACCTACAA
CGCCCGCAACGCGAAGGAACAGCTGAAGGCGTTCCGCACCAGCCCGGCTCTGCGCATCGCGGTGACGGTCGACATGATCG
CCACCGGCACCGATGTCAAACCGCTGGAGTGCGTGTTCTTCATGCGTGACGTGCGTTCGGCGCAGTACTTCGAGCAGATG
AAGGGCCGGGGTGCACGCACCATTCCCGACGCGGACTTCCAGGCGGTCACGCCGGACGCCACGACCAAGACCCGGTTCGT
CATCGTCGACGCGATCGGTGTCACCGAGCACGATTTCGTCGAACCCCCGCTGAACCGGCAGCGCACGGTGCCGCTGAAGA
AGCTGCTGGAGAAAGCCGCCAACCTGACGATCGCTGAGGACGAGGCGGCCACCCTGGCCTCACGCCTGGCCAAGCTCGAA
CTCGATCTGACCGATGCCGAGCGTGTCGAGCTCGATGAGGTGGCGGGGCAGCCGGTGCGCGAGATCATCCGGTCGTTGGT
GGATGCGGTCGAGCCGTCGGCCACAACTCCCGAAGCCATTGAAGCGGCCCTGGTGCCGATCGCGTCGAACCCGGAGCTGC
GCAACCGTGTCCTCGAACTTCGGGCCGCCCACGACCGCATCATCGATGAGGTCAGCGCCGATGAGCTGATCGAGGCCGGG
GGAGTGGTGGACCCCGGCAAGGCCCAGTCGATCGTAGAGTCGTGGACCGCGTACTCGAAGAGCACCGTGATGAGATCACG
GCGCTGCAGTTGGGATATGAGGCCAGCGAGCGACGCATCGACTTCGGCTTCATCGAGGGGTTGGCCGCGAGAATTGCTCG
GCCGCCGCACAATTGGACGCCTGATCTACTCTGGAACGCCTATGCCGCGGTCGACGGCCCGAAGGTGCACAAGAGCGCCA
CCCATGCGGTGA

Upstream 100 bases:

>100_bases
GGTGGGGTTGCAGCGGAGTAGACCGTCGCGAAGGTGCCCCGCAGGGCAGCGCGTAATCGCTCGCCGAACTTCGGTCACAG
TGGCTAACCTGTGACCCAGT

Downstream 100 bases:

>100_bases
CCGACCTTGTGTCCCTCGTCCGGTATGCCACCGGCGTCGAGGACGAACTCGTCCCCTACGGTGACCGGGTGCGGGAGAAG
TACGCGGCGTGGCTGACGCA

Product: type III restriction enzyme, res subunit

Products: NA

Alternate protein names: R.EcoKI [H]

Number of amino acids: Translated: 803; Mature: 802

Protein sequence:

>803_residues
MSASDDDARLPAEARARKLIDAQLTQAGWVVQDKQDLNLFAAQGVAVREVVMKPGHGRVDYLLYVDKAVVGVIEAKPVGT
PLSGVEWQSAMYADGLPADVRIAAKTRDGRLPFVFEASGVETHFTNGFEPESRARLIFNVPRPETLARYLREAETNPDYP
TWRAKVRKLPALDTAALRPAQIEAVNGVEQSLAGQQCNRSLVQMATGAGKTFTAVTQSYRLLKHGGFERILFLVDRNNLA
DQTLGEFQNYRTPDDGRRFTELYNVNKLSSAGLLGSTKVTISTIQRVFRFIKAGEVSDADDPDIDDYVPDAPVTVSYSEA
LPPETFDLVIVDEAHRSIYGVWRGVLEYFDAHVVGLTATPGKQTFAFFRQNLVSDYTYPESVADGVNVDFDVYRIRTKIS
DQGSHIDAGTIVPKVDRRTREQRLEALDDDLDYVPGQLDRAVTATDQIRTVLETFRDRLFTEIFPGRSTVPKTLIFAKDD
NHAEEIVRQVREVFGKGNDFAAKITYNARNAKEQLKAFRTSPALRIAVTVDMIATGTDVKPLECVFFMRDVRSAQYFEQM
KGRGARTIPDADFQAVTPDATTKTRFVIVDAIGVTEHDFVEPPLNRQRTVPLKKLLEKAANLTIAEDEAATLASRLAKLE
LDLTDAERVELDEVAGQPVREIIRSLVDAVEPSATTPEAIEAALVPIASNPELRNRVLELRAAHDRIIDEVSADELIEAG
GVVDPGKAQSIVESWTAYSKSTVMRSRRCSWDMRPASDASTSASSRGWPRELLGRRTIGRLIYSGTPMPRSTARRCTRAP
PMR

Sequences:

>Translated_803_residues
MSASDDDARLPAEARARKLIDAQLTQAGWVVQDKQDLNLFAAQGVAVREVVMKPGHGRVDYLLYVDKAVVGVIEAKPVGT
PLSGVEWQSAMYADGLPADVRIAAKTRDGRLPFVFEASGVETHFTNGFEPESRARLIFNVPRPETLARYLREAETNPDYP
TWRAKVRKLPALDTAALRPAQIEAVNGVEQSLAGQQCNRSLVQMATGAGKTFTAVTQSYRLLKHGGFERILFLVDRNNLA
DQTLGEFQNYRTPDDGRRFTELYNVNKLSSAGLLGSTKVTISTIQRVFRFIKAGEVSDADDPDIDDYVPDAPVTVSYSEA
LPPETFDLVIVDEAHRSIYGVWRGVLEYFDAHVVGLTATPGKQTFAFFRQNLVSDYTYPESVADGVNVDFDVYRIRTKIS
DQGSHIDAGTIVPKVDRRTREQRLEALDDDLDYVPGQLDRAVTATDQIRTVLETFRDRLFTEIFPGRSTVPKTLIFAKDD
NHAEEIVRQVREVFGKGNDFAAKITYNARNAKEQLKAFRTSPALRIAVTVDMIATGTDVKPLECVFFMRDVRSAQYFEQM
KGRGARTIPDADFQAVTPDATTKTRFVIVDAIGVTEHDFVEPPLNRQRTVPLKKLLEKAANLTIAEDEAATLASRLAKLE
LDLTDAERVELDEVAGQPVREIIRSLVDAVEPSATTPEAIEAALVPIASNPELRNRVLELRAAHDRIIDEVSADELIEAG
GVVDPGKAQSIVESWTAYSKSTVMRSRRCSWDMRPASDASTSASSRGWPRELLGRRTIGRLIYSGTPMPRSTARRCTRAP
PMR
>Mature_802_residues
SASDDDARLPAEARARKLIDAQLTQAGWVVQDKQDLNLFAAQGVAVREVVMKPGHGRVDYLLYVDKAVVGVIEAKPVGTP
LSGVEWQSAMYADGLPADVRIAAKTRDGRLPFVFEASGVETHFTNGFEPESRARLIFNVPRPETLARYLREAETNPDYPT
WRAKVRKLPALDTAALRPAQIEAVNGVEQSLAGQQCNRSLVQMATGAGKTFTAVTQSYRLLKHGGFERILFLVDRNNLAD
QTLGEFQNYRTPDDGRRFTELYNVNKLSSAGLLGSTKVTISTIQRVFRFIKAGEVSDADDPDIDDYVPDAPVTVSYSEAL
PPETFDLVIVDEAHRSIYGVWRGVLEYFDAHVVGLTATPGKQTFAFFRQNLVSDYTYPESVADGVNVDFDVYRIRTKISD
QGSHIDAGTIVPKVDRRTREQRLEALDDDLDYVPGQLDRAVTATDQIRTVLETFRDRLFTEIFPGRSTVPKTLIFAKDDN
HAEEIVRQVREVFGKGNDFAAKITYNARNAKEQLKAFRTSPALRIAVTVDMIATGTDVKPLECVFFMRDVRSAQYFEQMK
GRGARTIPDADFQAVTPDATTKTRFVIVDAIGVTEHDFVEPPLNRQRTVPLKKLLEKAANLTIAEDEAATLASRLAKLEL
DLTDAERVELDEVAGQPVREIIRSLVDAVEPSATTPEAIEAALVPIASNPELRNRVLELRAAHDRIIDEVSADELIEAGG
VVDPGKAQSIVESWTAYSKSTVMRSRRCSWDMRPASDASTSASSRGWPRELLGRRTIGRLIYSGTPMPRSTARRCTRAPP
MR

Specific function: The EcoKI enzyme recognizes 5'-AACN(6)GTGC-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG4096

COG function: function code V; Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI226510991, Length=660, Percent_Identity=27.4242424242424, Blast_Score=176, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR021943
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR007409 [H]

Pfam domain/function: PF12082 DUF3559; PF00271 Helicase_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 89108; Mature: 88976

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASDDDARLPAEARARKLIDAQLTQAGWVVQDKQDLNLFAAQGVAVREVVMKPGHGRVD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHCCHHHHHHHHCCCCCCEE
YLLYVDKAVVGVIEAKPVGTPLSGVEWQSAMYADGLPADVRIAAKTRDGRLPFVFEASGV
EEEEECHHHHHHEECCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCEEEECCCC
ETHFTNGFEPESRARLIFNVPRPETLARYLREAETNPDYPTWRAKVRKLPALDTAALRPA
CCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHCCCH
QIEAVNGVEQSLAGQQCNRSLVQMATGAGKTFTAVTQSYRLLKHGGFERILFLVDRNNLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCH
DQTLGEFQNYRTPDDGRRFTELYNVNKLSSAGLLGSTKVTISTIQRVFRFIKAGEVSDAD
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
DPDIDDYVPDAPVTVSYSEALPPETFDLVIVDEAHRSIYGVWRGVLEYFDAHVVGLTATP
CCCCHHCCCCCCEEEEHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCEEEEEECCC
GKQTFAFFRQNLVSDYTYPESVADGVNVDFDVYRIRTKISDQGSHIDAGTIVPKVDRRTR
CHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEEEEHHCCCCCCCCCCCCCCCHHHHHH
EQRLEALDDDLDYVPGQLDRAVTATDQIRTVLETFRDRLFTEIFPGRSTVPKTLIFAKDD
HHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
NHAEEIVRQVREVFGKGNDFAAKITYNARNAKEQLKAFRTSPALRIAVTVDMIATGTDVK
CHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCC
PLECVFFMRDVRSAQYFEQMKGRGARTIPDADFQAVTPDATTKTRFVIVDAIGVTEHDFV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEEEEECCCCCCCC
EPPLNRQRTVPLKKLLEKAANLTIAEDEAATLASRLAKLELDLTDAERVELDEVAGQPVR
CCCCCCCCCCHHHHHHHHHHCCEEECHHHHHHHHHHHHHEECCCCCHHHHHHHHCCHHHH
EIIRSLVDAVEPSATTPEAIEAALVPIASNPELRNRVLELRAAHDRIIDEVSADELIEAG
HHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
GVVDPGKAQSIVESWTAYSKSTVMRSRRCSWDMRPASDASTSASSRGWPRELLGRRTIGR
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LIYSGTPMPRSTARRCTRAPPMR
HHHCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SASDDDARLPAEARARKLIDAQLTQAGWVVQDKQDLNLFAAQGVAVREVVMKPGHGRVD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHCCHHHHHHHHCCCCCCEE
YLLYVDKAVVGVIEAKPVGTPLSGVEWQSAMYADGLPADVRIAAKTRDGRLPFVFEASGV
EEEEECHHHHHHEECCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCEEEECCCC
ETHFTNGFEPESRARLIFNVPRPETLARYLREAETNPDYPTWRAKVRKLPALDTAALRPA
CCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHCCCH
QIEAVNGVEQSLAGQQCNRSLVQMATGAGKTFTAVTQSYRLLKHGGFERILFLVDRNNLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCH
DQTLGEFQNYRTPDDGRRFTELYNVNKLSSAGLLGSTKVTISTIQRVFRFIKAGEVSDAD
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
DPDIDDYVPDAPVTVSYSEALPPETFDLVIVDEAHRSIYGVWRGVLEYFDAHVVGLTATP
CCCCHHCCCCCCEEEEHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCEEEEEECCC
GKQTFAFFRQNLVSDYTYPESVADGVNVDFDVYRIRTKISDQGSHIDAGTIVPKVDRRTR
CHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEEEEHHCCCCCCCCCCCCCCCHHHHHH
EQRLEALDDDLDYVPGQLDRAVTATDQIRTVLETFRDRLFTEIFPGRSTVPKTLIFAKDD
HHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
NHAEEIVRQVREVFGKGNDFAAKITYNARNAKEQLKAFRTSPALRIAVTVDMIATGTDVK
CHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCC
PLECVFFMRDVRSAQYFEQMKGRGARTIPDADFQAVTPDATTKTRFVIVDAIGVTEHDFV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEEEEECCCCCCCC
EPPLNRQRTVPLKKLLEKAANLTIAEDEAATLASRLAKLELDLTDAERVELDEVAGQPVR
CCCCCCCCCCHHHHHHHHHHCCEEECHHHHHHHHHHHHHEECCCCCHHHHHHHHCCHHHH
EIIRSLVDAVEPSATTPEAIEAALVPIASNPELRNRVLELRAAHDRIIDEVSADELIEAG
HHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
GVVDPGKAQSIVESWTAYSKSTVMRSRRCSWDMRPASDASTSASSRGWPRELLGRRTIGR
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LIYSGTPMPRSTARRCTRAPPMR
HHHCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3323532; 7610040; 9278503; 1650347 [H]