Click here to switch to the map view.

The map label for this gene is pdhC [H]

Identifier: 108800590

GI number: 108800590

Start: 3868819

End: 3869973

Strand: Reverse

Name: pdhC [H]

Synonym: Mmcs_3624

Alternate gene names: 108800590

Gene position: 3869973-3868819 (Counterclockwise)

Preceding gene: 108800591

Following gene: 108800587

Centisome position: 67.83

GC content: 70.82

Gene sequence:

>1155_bases
ATGACCGTGCAGGAGTTCCTGGTGCCGGATCTGGGTGAGGGTCTGCAGGACGCGACGATCACCTCGTGGGCGGTCGATGT
CGGTGACGAGGTCGAGCTGAACCAGACGCTGTGCACCGTCGAGACGAACAAGGCGGAGGTCGAGATCCCCAGCCCGTACG
CCGGCCGGGTGGTCGAACGCGGGGGAGACGAGGGTCAGACGCTCGACGTGGGATCCCTGCTGGTGCGGATCGCGACATCA
GCGGATGAACCGGTGCCGCAACGCAAATCGGTACTGGTCGGATACGGCGCCGATGAGGCGATGGATACCAGCAGGCGCCG
GTCGGCGCGGCCGCGCGCCAAACCGCCGGTGCGCAAGCTGGCGGCCGAACTGGACGTGGATCTCAACGCGGTCAGCGCAT
CGGGACCCGACGGCGTGGTCACCCGCGAGGACGTGCAGCGTGCGGCGGAAGGCTGGGCGCCGGCCGCGGAGCAGGTCGCG
GTGCGGGGTGTCCAGGCGGAGATGGCGCGACGGATGTCGTTGTCGCGGAGGGAGATCCCGGATGCCCACGCCAGCGTCAC
GGTCGACGGCAGCGCACTGCTGCGGCTGCGGGACAGACTGCTCGGCACCGACCCGCCGGTGACGCCCTTCGTGCTGACGC
TGCGACTGCTGACGCTGGCGCTGCGGCACCAGCCGGCGCTAAACGCCACCTGGATCGAGACGGCGGAGGGGCCGCAGATC
CACCGGCACACTGCAGTCCACCTCGGATTCGGTGTCGCCGCGCCGCGCGGGCTGCTGGTGCCGGTGATCGCCGACGCGCA
GGACATGACCACGCGTGGACTCGCGGCCGCGGTGGGGCGGCTGGTGGCGGAGGCTCGCGCCGGACGGGTGCGGCCGGCGG
AGCTGTCGGGGTCGACGTTCACCGTCTCGAATTTCGGTGCGCTCGGGCTCGACGAGGGCGTGCCGGTGATCAACCATCCG
GAGGCTGCGATCCTGGGCATGGGTTCGTTGAAGCCGCGCGCGGTGGTCGTCGACGGTGAGGTGGTCGCCCGGCCGACGAT
GACGTTGACGTGCGCCTTCGACCATCGCATCGCCGACGGGGCCACCGTGGCGGCCTTCCTGGGCGAGTTGCGCGAGCTCG
TCGAAGCGCCGGAGCTCGCGCTGCTCGACCTGTGA

Upstream 100 bases:

>100_bases
CCGGGTTCGACACCCCGTATCCACCGGCCCGGCTGGAGAAGCTGTGGTTACCGGGGGTGGACCGGTTGCTCGACTGCGTC
CAGCGGACCCTGGAGATGCC

Downstream 100 bases:

>100_bases
CCTACTTGCGTTTGGCCGCGGCGAGGCGGCGGGCGAACTCCGGCGATTCGATGGACGCCGCCTGAGGACCGAGTTCGATG
TCGACCGCCGTGCGGTGCTG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTVQEFLVPDLGEGLQDATITSWAVDVGDEVELNQTLCTVETNKAEVEIPSPYAGRVVERGGDEGQTLDVGSLLVRIATS
ADEPVPQRKSVLVGYGADEAMDTSRRRSARPRAKPPVRKLAAELDVDLNAVSASGPDGVVTREDVQRAAEGWAPAAEQVA
VRGVQAEMARRMSLSRREIPDAHASVTVDGSALLRLRDRLLGTDPPVTPFVLTLRLLTLALRHQPALNATWIETAEGPQI
HRHTAVHLGFGVAAPRGLLVPVIADAQDMTTRGLAAAVGRLVAEARAGRVRPAELSGSTFTVSNFGALGLDEGVPVINHP
EAAILGMGSLKPRAVVVDGEVVARPTMTLTCAFDHRIADGATVAAFLGELRELVEAPELALLDL

Sequences:

>Translated_384_residues
MTVQEFLVPDLGEGLQDATITSWAVDVGDEVELNQTLCTVETNKAEVEIPSPYAGRVVERGGDEGQTLDVGSLLVRIATS
ADEPVPQRKSVLVGYGADEAMDTSRRRSARPRAKPPVRKLAAELDVDLNAVSASGPDGVVTREDVQRAAEGWAPAAEQVA
VRGVQAEMARRMSLSRREIPDAHASVTVDGSALLRLRDRLLGTDPPVTPFVLTLRLLTLALRHQPALNATWIETAEGPQI
HRHTAVHLGFGVAAPRGLLVPVIADAQDMTTRGLAAAVGRLVAEARAGRVRPAELSGSTFTVSNFGALGLDEGVPVINHP
EAAILGMGSLKPRAVVVDGEVVARPTMTLTCAFDHRIADGATVAAFLGELRELVEAPELALLDL
>Mature_383_residues
TVQEFLVPDLGEGLQDATITSWAVDVGDEVELNQTLCTVETNKAEVEIPSPYAGRVVERGGDEGQTLDVGSLLVRIATSA
DEPVPQRKSVLVGYGADEAMDTSRRRSARPRAKPPVRKLAAELDVDLNAVSASGPDGVVTREDVQRAAEGWAPAAEQVAV
RGVQAEMARRMSLSRREIPDAHASVTVDGSALLRLRDRLLGTDPPVTPFVLTLRLLTLALRHQPALNATWIETAEGPQIH
RHTAVHLGFGVAAPRGLLVPVIADAQDMTTRGLAAAVGRLVAEARAGRVRPAELSGSTFTVSNFGALGLDEGVPVINHPE
AAILGMGSLKPRAVVVDGEVVARPTMTLTCAFDHRIADGATVAAFLGELRELVEAPELALLDL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=28.2051282051282, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI31711992, Length=295, Percent_Identity=28.8135593220339, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI203098753, Length=225, Percent_Identity=28.8888888888889, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI203098816, Length=225, Percent_Identity=28.8888888888889, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI19923748, Length=166, Percent_Identity=36.144578313253, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=31.875, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1786946, Length=419, Percent_Identity=27.2076372315036, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786305, Length=415, Percent_Identity=28.1927710843373, Blast_Score=131, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI25146366, Length=410, Percent_Identity=26.8292682926829, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=26.2910798122066, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17560088, Length=432, Percent_Identity=27.3148148148148, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=27.8761061946903, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=396, Percent_Identity=29.040404040404, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=22.0489977728285, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24645909, Length=161, Percent_Identity=37.888198757764, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24582497, Length=236, Percent_Identity=26.6949152542373, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129315, Length=236, Percent_Identity=26.6949152542373, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 40677; Mature: 40546

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQEFLVPDLGEGLQDATITSWAVDVGDEVELNQTLCTVETNKAEVEIPSPYAGRVVER
CCHHHHHCCHHCCCCCCCEEEEEEECCCCCEECCCEEEEEECCCEEEECCCCCHHHHHHC
GGDEGQTLDVGSLLVRIATSADEPVPQRKSVLVGYGADEAMDTSRRRSARPRAKPPVRKL
CCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHH
AAELDVDLNAVSASGPDGVVTREDVQRAAEGWAPAAEQVAVRGVQAEMARRMSLSRREIP
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DAHASVTVDGSALLRLRDRLLGTDPPVTPFVLTLRLLTLALRHQPALNATWIETAEGPQI
CCCCEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCE
HRHTAVHLGFGVAAPRGLLVPVIADAQDMTTRGLAAAVGRLVAEARAGRVRPAELSGSTF
EEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
TVSNFGALGLDEGVPVINHPEAAILGMGSLKPRAVVVDGEVVARPTMTLTCAFDHRIADG
EECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCEEECCCEEEEEEECCCCCCC
ATVAAFLGELRELVEAPELALLDL
HHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
TVQEFLVPDLGEGLQDATITSWAVDVGDEVELNQTLCTVETNKAEVEIPSPYAGRVVER
CHHHHHCCHHCCCCCCCEEEEEEECCCCCEECCCEEEEEECCCEEEECCCCCHHHHHHC
GGDEGQTLDVGSLLVRIATSADEPVPQRKSVLVGYGADEAMDTSRRRSARPRAKPPVRKL
CCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHH
AAELDVDLNAVSASGPDGVVTREDVQRAAEGWAPAAEQVAVRGVQAEMARRMSLSRREIP
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DAHASVTVDGSALLRLRDRLLGTDPPVTPFVLTLRLLTLALRHQPALNATWIETAEGPQI
CCCCEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCE
HRHTAVHLGFGVAAPRGLLVPVIADAQDMTTRGLAAAVGRLVAEARAGRVRPAELSGSTF
EEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
TVSNFGALGLDEGVPVINHPEAAILGMGSLKPRAVVVDGEVVARPTMTLTCAFDHRIADG
EECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCEEECCCEEEEEEECCCCCCC
ATVAAFLGELRELVEAPELALLDL
HHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA