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The map label for this gene is 108800586

Identifier: 108800586

GI number: 108800586

Start: 3864705

End: 3866186

Strand: Reverse

Name: 108800586

Synonym: Mmcs_3620

Alternate gene names: NA

Gene position: 3866186-3864705 (Counterclockwise)

Preceding gene: 108800587

Following gene: 108800585

Centisome position: 67.76

GC content: 70.58

Gene sequence:

>1482_bases
GTGGCGACGAGCGACGTGCCGGAATTGGATGCCGCGGGGTTGCCCGAAGAGCTCAGCCCGTTCGACCAGATCCTGCACCG
CGGCGAGGCCAACCCGCGGACACGCTCGGGCATCCTGACCATCGAGATCCTCGACACCACGCCGGACTGGGACCGGTTTC
GCACCCGTTTCGAACACGCCTCTCGCAAAGTCCTGCGCCTGCGCCAGAAGGTCGTGACGCCGACCCTGCCGACGGCCGCA
CCGCGGTGGGTCGTCGACCCCGACTTCAACCTGGACTTCCACGTCCGCCGGGTCCGCATCCCGGAGCCCGGCAACATGCG
GCAGCTGATCGATCTCGCCGAGATCGCCCTGCAGTCGCCCTTGGACATCTCGCGCCCGCTGTGGACCGCCACGCTCGTCG
AAGGGCTCGAGGGTGGTCGCGCCGCGACGATGTTGCACTTCAGCCACGCGGTGACCGACGGAGTCGGCGGTGTCGAGATG
TTCGCCAGCGTCTACGACCTGGAACGCGACCCGCCGCCGAGCGATGCGCCGCCGCTGCCGATTCCGCAGGACCTCTCGCC
GAACGACCTCATGCGTCAGGGGCTCAACCGGCTGCCGTTCTCCCTCCTCGGCGGCGTCCGCAACGCGCTGGGCGGCGCCG
CGAGCGCGGTCGGCAAGGTGGTGCGCGACCCCGTCTCGTCCGTCGGCGGCGTGGTCGACTACGCCCGCTCCGGCGCGCGT
GTGGTGGGCTCGGTCGCCGAACCCTCGCCGCTGCTGCGCCGGCGCAGCCTCTCGACACGCAGCGAGGCGATCGACATGTC
CCTCGGCGACCTGCACAAGGCGGCCAAGGCGGCCGGCGGCTCCATCAACGACGCGTACCTGGCGGGCGTGTGCGGGGCGC
TGCGGCTCTACCACGACGCCTTCGGCCTACCCGTCGCCACGCTGCCGATGGCCGTCCCCGTGAGCCTGCGGTCCGACGCC
GATCCCGCGGGCGGCAACCGCTTCGTCGGGGTGAACCTCGCCGCACCCATCGGCGTCACGGATCCGCGCGAGCGCATCGC
CAAGCTGCGCGCACAGATGACGCGAAAGCGCGAGGAGCGCGCCCTCGACATGATCGGCGCCGTGGCACCCGTCGCGAGCC
TGCTACCCGATACGGTGCTCGAGGCGATGGCCGGTTCGGTCGTCAACTCCGACGTCCAGGCCAGCAACGTGCCCGTCTAC
GCTGGTGACACCTACATCGCGGGCGCAAAGATCCTCCGCCAGTACGGCATCGGACCGCTGCCCGGTGTGGCGATGATGGT
GGTTCTGGTGTCGCGCTCCGGCTTCTGCACGGTCACCGCCCGCTACGACCGCGCGTCCATCACCGACCCCGACCTGTTCG
CCGAGTGCCTGCTCGCCGGTTTCGACGAGGTGCTCGCCATCGGCGGTCCCGGCCGCGCCAGCCCGGCGTCGTTCACCCTG
GAGTCCGAAAAACCGTCACCGAATGGGAGTGCGCCACAATGA

Upstream 100 bases:

>100_bases
GCAGCGCGGGTGAGTGTATCTCGCCTCCCAGTGGGCAGCCGCGGACTAGCGTGGTGTCAGCAGTCATGCGAGCAGGCACT
GTCGACAGGAGGGCTTCAGG

Downstream 100 bases:

>100_bases
GTCCGGACGACGGTCAGCAGTCCGAGCGGCGTCCCATGCGTCTGCCCGGGTCGGTCGCGGAGATCCTCGCCGCACCGGAG
GGGCCGCAGGTCGGCGCGTT

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv2484c/MT2557 [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MATSDVPELDAAGLPEELSPFDQILHRGEANPRTRSGILTIEILDTTPDWDRFRTRFEHASRKVLRLRQKVVTPTLPTAA
PRWVVDPDFNLDFHVRRVRIPEPGNMRQLIDLAEIALQSPLDISRPLWTATLVEGLEGGRAATMLHFSHAVTDGVGGVEM
FASVYDLERDPPPSDAPPLPIPQDLSPNDLMRQGLNRLPFSLLGGVRNALGGAASAVGKVVRDPVSSVGGVVDYARSGAR
VVGSVAEPSPLLRRRSLSTRSEAIDMSLGDLHKAAKAAGGSINDAYLAGVCGALRLYHDAFGLPVATLPMAVPVSLRSDA
DPAGGNRFVGVNLAAPIGVTDPRERIAKLRAQMTRKREERALDMIGAVAPVASLLPDTVLEAMAGSVVNSDVQASNVPVY
AGDTYIAGAKILRQYGIGPLPGVAMMVVLVSRSGFCTVTARYDRASITDPDLFAECLLAGFDEVLAIGGPGRASPASFTL
ESEKPSPNGSAPQ

Sequences:

>Translated_493_residues
MATSDVPELDAAGLPEELSPFDQILHRGEANPRTRSGILTIEILDTTPDWDRFRTRFEHASRKVLRLRQKVVTPTLPTAA
PRWVVDPDFNLDFHVRRVRIPEPGNMRQLIDLAEIALQSPLDISRPLWTATLVEGLEGGRAATMLHFSHAVTDGVGGVEM
FASVYDLERDPPPSDAPPLPIPQDLSPNDLMRQGLNRLPFSLLGGVRNALGGAASAVGKVVRDPVSSVGGVVDYARSGAR
VVGSVAEPSPLLRRRSLSTRSEAIDMSLGDLHKAAKAAGGSINDAYLAGVCGALRLYHDAFGLPVATLPMAVPVSLRSDA
DPAGGNRFVGVNLAAPIGVTDPRERIAKLRAQMTRKREERALDMIGAVAPVASLLPDTVLEAMAGSVVNSDVQASNVPVY
AGDTYIAGAKILRQYGIGPLPGVAMMVVLVSRSGFCTVTARYDRASITDPDLFAECLLAGFDEVLAIGGPGRASPASFTL
ESEKPSPNGSAPQ
>Mature_492_residues
ATSDVPELDAAGLPEELSPFDQILHRGEANPRTRSGILTIEILDTTPDWDRFRTRFEHASRKVLRLRQKVVTPTLPTAAP
RWVVDPDFNLDFHVRRVRIPEPGNMRQLIDLAEIALQSPLDISRPLWTATLVEGLEGGRAATMLHFSHAVTDGVGGVEMF
ASVYDLERDPPPSDAPPLPIPQDLSPNDLMRQGLNRLPFSLLGGVRNALGGAASAVGKVVRDPVSSVGGVVDYARSGARV
VGSVAEPSPLLRRRSLSTRSEAIDMSLGDLHKAAKAAGGSINDAYLAGVCGALRLYHDAFGLPVATLPMAVPVSLRSDAD
PAGGNRFVGVNLAAPIGVTDPRERIAKLRAQMTRKREERALDMIGAVAPVASLLPDTVLEAMAGSVVNSDVQASNVPVYA
GDTYIAGAKILRQYGIGPLPGVAMMVVLVSRSGFCTVTARYDRASITDPDLFAECLLAGFDEVLAIGGPGRASPASFTLE
SEKPSPNGSAPQ

Specific function: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 52358; Mature: 52227

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSDVPELDAAGLPEELSPFDQILHRGEANPRTRSGILTIEILDTTPDWDRFRTRFEHA
CCCCCCCCCCCCCCCHHCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
SRKVLRLRQKVVTPTLPTAAPRWVVDPDFNLDFHVRRVRIPEPGNMRQLIDLAEIALQSP
HHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCC
LDISRPLWTATLVEGLEGGRAATMLHFSHAVTDGVGGVEMFASVYDLERDPPPSDAPPLP
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCC
IPQDLSPNDLMRQGLNRLPFSLLGGVRNALGGAASAVGKVVRDPVSSVGGVVDYARSGAR
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VVGSVAEPSPLLRRRSLSTRSEAIDMSLGDLHKAAKAAGGSINDAYLAGVCGALRLYHDA
EECCCCCCHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
FGLPVATLPMAVPVSLRSDADPAGGNRFVGVNLAAPIGVTDPRERIAKLRAQMTRKREER
HCCCHHHHCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
ALDMIGAVAPVASLLPDTVLEAMAGSVVNSDVQASNVPVYAGDTYIAGAKILRQYGIGPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCC
PGVAMMVVLVSRSGFCTVTARYDRASITDPDLFAECLLAGFDEVLAIGGPGRASPASFTL
CHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHEEECCCCCCCCCCEEE
ESEKPSPNGSAPQ
CCCCCCCCCCCCC
>Mature Secondary Structure 
ATSDVPELDAAGLPEELSPFDQILHRGEANPRTRSGILTIEILDTTPDWDRFRTRFEHA
CCCCCCCCCCCCCCHHCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
SRKVLRLRQKVVTPTLPTAAPRWVVDPDFNLDFHVRRVRIPEPGNMRQLIDLAEIALQSP
HHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCC
LDISRPLWTATLVEGLEGGRAATMLHFSHAVTDGVGGVEMFASVYDLERDPPPSDAPPLP
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCC
IPQDLSPNDLMRQGLNRLPFSLLGGVRNALGGAASAVGKVVRDPVSSVGGVVDYARSGAR
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VVGSVAEPSPLLRRRSLSTRSEAIDMSLGDLHKAAKAAGGSINDAYLAGVCGALRLYHDA
EECCCCCCHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
FGLPVATLPMAVPVSLRSDADPAGGNRFVGVNLAAPIGVTDPRERIAKLRAQMTRKREER
HCCCHHHHCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
ALDMIGAVAPVASLLPDTVLEAMAGSVVNSDVQASNVPVYAGDTYIAGAKILRQYGIGPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCC
PGVAMMVVLVSRSGFCTVTARYDRASITDPDLFAECLLAGFDEVLAIGGPGRASPASFTL
CHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHEEECCCCCCCCCCEEE
ESEKPSPNGSAPQ
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]