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The map label for this gene is folC [H]

Identifier: 108800523

GI number: 108800523

Start: 3784718

End: 3786121

Strand: Reverse

Name: folC [H]

Synonym: Mmcs_3557

Alternate gene names: 108800523

Gene position: 3786121-3784718 (Counterclockwise)

Preceding gene: 108800524

Following gene: 108800522

Centisome position: 66.36

GC content: 71.3

Gene sequence:

>1404_bases
ATGAGCCGGCCCGAGCCCACCCCCGACGAGATCGCGGCGCTGCTGCAGGTCGAGCACCTGCTCGACGCCCGCTGGCCGGA
AACCAAGATCGAGCCGAGCACCGCGCGGATCAGCGCGCTGCTCGAGATGCTCGGCAACCCCCAGCGCGGCTATCCGTCGA
TCCACATCGCGGGCACGAACGGCAAGACGTCGGTGGCCCGGATGGTCGATGCGCTGCTGACCGCGCTCAGCCGGCGCACC
GGACGCACCACCAGCCCGCATCTGCAGTCGGCGGTCGAGCGCGTCGCGATCGACGGCGAACCGATCTCCCCGGCCCGATA
CGTCGAGGTCTACCGCGAGATCGAACCGTTCGTCCTGATGGTCGACGAACGGTCCGAAGAGGCAGGCGGACCGCGGCTGA
GCAAGTTCGAGGTGCTCACCGCGATGGCCTTCGCCGCATTCGCCGACGCACCCGTGGACGTCGCGGTCGTGGAGGTCGGT
ATGGGTGGCCGGTGGGATGCGACCAACGTGGTCAACGCCCCGGTCGCGGTGATCACGCCGATCGGCATCGACCACGCCGA
CTACCTCGGGGACACGATCGCCGAGATCGCGGGGGAGAAGGCGGGCATCATCACCCGCCAGCCCGACGACCTCGTCCCGA
CGGACACGGTCGCGGTGATCGGGCGCCAGGCACCCGAGGCCATGGAGGTGCTGCTGGCCCAGGCCGTCCGCGCGGACGCC
GCGGTGGCCCGCGAGGACTCCGAATTCGCGGTGCTGTCCCGCCAGGTCGCCGTCGGCGGCCAGCTTCTCGAACTGCAGGG
CCTCGGCGGTGTGTACCCGGAGATCTTCCTGCCGCTGCACGGTGAACACCAGGCCCACAACGCGATACTCGCCCTAGCCG
CGGTCGAGGCGTTCTTCGGCGCGGGCGCGGAGCGCCAACTCGACATCGACGCCGTGCGGGCCGGATTCGCCGCCGCGGCC
AGTCCCGGCCGGTTGGAGCGGCTGCGCAGCGCGCCGACGGTGTTCATCGACGCCGCGCACAACCCCGCGGGCGCCGCCGC
GCTCGCCGATGCGCTGCAGACCGAATTCGACTTCCGGTTCCTGGTCGGGGTGGTGTCGGTGATGGGGGACAAGGACGTCG
ACGGCATCCTCGCGGCGCTGGAACCGGCGTTCGACCAGATCGTCGTCACCCACAACGGTTCGCCGCGCGCGCTCGACGTC
GAGGCGCTCGCCGTACGCGCCGAGGAGCGTTTCGGCCCGGAACGGGTGATCCGGGCGGCCACGCTGCCGGACGCCATCGA
GACGGCCACCGCGGTGGTCGAGGACTCCGGCGGCGAAGGACTCGCCGGTGCCGGGATGGTCATCACCGGGTCCGTGGTCA
CCGCGGGTGCCGCGAGGACCCTGTTCGGACGGGATCCGCAATGA

Upstream 100 bases:

>100_bases
TCGCCAAGGCGCCCGCGGAGGTGGTCGACAAGATCCGCGGCCGCCAGCAGGTGGCGCGCGAGGAGGTCGAGCGCATCACG
GCTCGCCTGAGCGCGTTGCG

Downstream 100 bases:

>100_bases
GCGAGCAGACCCCGTCCGGTCAGCCCCAGGCGCCCGACCCGTGGCGCAGCTTCCGCGGCGTGATGGCCGGCACGCTCATC
CTCGAGGCCATCGTCGTACT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSRPEPTPDEIAALLQVEHLLDARWPETKIEPSTARISALLEMLGNPQRGYPSIHIAGTNGKTSVARMVDALLTALSRRT
GRTTSPHLQSAVERVAIDGEPISPARYVEVYREIEPFVLMVDERSEEAGGPRLSKFEVLTAMAFAAFADAPVDVAVVEVG
MGGRWDATNVVNAPVAVITPIGIDHADYLGDTIAEIAGEKAGIITRQPDDLVPTDTVAVIGRQAPEAMEVLLAQAVRADA
AVAREDSEFAVLSRQVAVGGQLLELQGLGGVYPEIFLPLHGEHQAHNAILALAAVEAFFGAGAERQLDIDAVRAGFAAAA
SPGRLERLRSAPTVFIDAAHNPAGAAALADALQTEFDFRFLVGVVSVMGDKDVDGILAALEPAFDQIVVTHNGSPRALDV
EALAVRAEERFGPERVIRAATLPDAIETATAVVEDSGGEGLAGAGMVITGSVVTAGAARTLFGRDPQ

Sequences:

>Translated_467_residues
MSRPEPTPDEIAALLQVEHLLDARWPETKIEPSTARISALLEMLGNPQRGYPSIHIAGTNGKTSVARMVDALLTALSRRT
GRTTSPHLQSAVERVAIDGEPISPARYVEVYREIEPFVLMVDERSEEAGGPRLSKFEVLTAMAFAAFADAPVDVAVVEVG
MGGRWDATNVVNAPVAVITPIGIDHADYLGDTIAEIAGEKAGIITRQPDDLVPTDTVAVIGRQAPEAMEVLLAQAVRADA
AVAREDSEFAVLSRQVAVGGQLLELQGLGGVYPEIFLPLHGEHQAHNAILALAAVEAFFGAGAERQLDIDAVRAGFAAAA
SPGRLERLRSAPTVFIDAAHNPAGAAALADALQTEFDFRFLVGVVSVMGDKDVDGILAALEPAFDQIVVTHNGSPRALDV
EALAVRAEERFGPERVIRAATLPDAIETATAVVEDSGGEGLAGAGMVITGSVVTAGAARTLFGRDPQ
>Mature_466_residues
SRPEPTPDEIAALLQVEHLLDARWPETKIEPSTARISALLEMLGNPQRGYPSIHIAGTNGKTSVARMVDALLTALSRRTG
RTTSPHLQSAVERVAIDGEPISPARYVEVYREIEPFVLMVDERSEEAGGPRLSKFEVLTAMAFAAFADAPVDVAVVEVGM
GGRWDATNVVNAPVAVITPIGIDHADYLGDTIAEIAGEKAGIITRQPDDLVPTDTVAVIGRQAPEAMEVLLAQAVRADAA
VAREDSEFAVLSRQVAVGGQLLELQGLGGVYPEIFLPLHGEHQAHNAILALAAVEAFFGAGAERQLDIDAVRAGFAAAAS
PGRLERLRSAPTVFIDAAHNPAGAAALADALQTEFDFRFLVGVVSVMGDKDVDGILAALEPAFDQIVVTHNGSPRALDVE
ALAVRAEERFGPERVIRAATLPDAIETATAVVEDSGGEGLAGAGMVITGSVVTAGAARTLFGRDPQ

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=371, Percent_Identity=30.7277628032345, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI66932990, Length=371, Percent_Identity=30.7277628032345, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788654, Length=349, Percent_Identity=31.2320916905444, Blast_Score=133, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=185, Percent_Identity=37.2972972972973, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71984923, Length=185, Percent_Identity=37.2972972972973, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17553150, Length=185, Percent_Identity=37.2972972972973, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323760, Length=373, Percent_Identity=30.8310991957105, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6324815, Length=477, Percent_Identity=26.6247379454927, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6322718, Length=445, Percent_Identity=22.4719101123595, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24641571, Length=179, Percent_Identity=36.8715083798883, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24581568, Length=197, Percent_Identity=31.9796954314721, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 49065; Mature: 48934

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPEPTPDEIAALLQVEHLLDARWPETKIEPSTARISALLEMLGNPQRGYPSIHIAGTN
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCC
GKTSVARMVDALLTALSRRTGRTTSPHLQSAVERVAIDGEPISPARYVEVYREIEPFVLM
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEE
VDERSEEAGGPRLSKFEVLTAMAFAAFADAPVDVAVVEVGMGGRWDATNVVNAPVAVITP
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEC
IGIDHADYLGDTIAEIAGEKAGIITRQPDDLVPTDTVAVIGRQAPEAMEVLLAQAVRADA
CCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
AVAREDSEFAVLSRQVAVGGQLLELQGLGGVYPEIFLPLHGEHQAHNAILALAAVEAFFG
HHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
AGAERQLDIDAVRAGFAAAASPGRLERLRSAPTVFIDAAHNPAGAAALADALQTEFDFRF
CCCCCCCCHHHHHHCHHHCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCHHH
LVGVVSVMGDKDVDGILAALEPAFDQIVVTHNGSPRALDVEALAVRAEERFGPERVIRAA
HHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHHHHCCHHHHHHHH
TLPDAIETATAVVEDSGGEGLAGAGMVITGSVVTAGAARTLFGRDPQ
CCCHHHHHHHHHEECCCCCCCCCCCEEEECCHHHCCCHHHCCCCCCC
>Mature Secondary Structure 
SRPEPTPDEIAALLQVEHLLDARWPETKIEPSTARISALLEMLGNPQRGYPSIHIAGTN
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCC
GKTSVARMVDALLTALSRRTGRTTSPHLQSAVERVAIDGEPISPARYVEVYREIEPFVLM
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEE
VDERSEEAGGPRLSKFEVLTAMAFAAFADAPVDVAVVEVGMGGRWDATNVVNAPVAVITP
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEC
IGIDHADYLGDTIAEIAGEKAGIITRQPDDLVPTDTVAVIGRQAPEAMEVLLAQAVRADA
CCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
AVAREDSEFAVLSRQVAVGGQLLELQGLGGVYPEIFLPLHGEHQAHNAILALAAVEAFFG
HHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
AGAERQLDIDAVRAGFAAAASPGRLERLRSAPTVFIDAAHNPAGAAALADALQTEFDFRF
CCCCCCCCHHHHHHCHHHCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCHHH
LVGVVSVMGDKDVDGILAALEPAFDQIVVTHNGSPRALDVEALAVRAEERFGPERVIRAA
HHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHHHHCCHHHHHHHH
TLPDAIETATAVVEDSGGEGLAGAGMVITGSVVTAGAARTLFGRDPQ
CCCHHHHHHHHHEECCCCCCCCCCCEEEECCHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]