BacMap

Definition	Mycobacterium sp. MCS chromosome, complete genome.
Accession	NC_008146
Length	5,705,448

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The map label for this gene is dnaJ2 [H]

Identifier: 108800425

GI number: 108800425

Start: 3668861

End: 3670015

Strand: Reverse

Name: dnaJ2 [H]

Synonym: Mmcs_3459

Alternate gene names: 108800425

Gene position: 3670015-3668861 (Counterclockwise)

Preceding gene: 108800426

Following gene: 108800424

Centisome position: 64.32

GC content: 71.43

Gene sequence:

>1155_bases
GTGGCACGCGATTATTACGGCCTGCTCGGAGTGAGCAAGGGCGCCAGCGATCAGGAGATCAAGCGCGCCTATCGCAAGCT
TGCCCGCGAGCTGCACCCCGACGTCAACCCCGACGAGGAGGCGCAGGCGCGGTTCAAGGAGATCAGCGCCGCCTATGAGG
TGCTGTCCGATCCCGAGAAGCGGCGGATCGTCGACCTCGGTGGTGACCCGCTCGAGAGTGCGGGCGCCTCGGGCGGGAAT
GGATTCGGCGCTGGTTTCGGTGGGCTGGGCGATGTGTTCGAGGCGTTCTTCGGCGGTGGCGGCGGCTCCCGCGGACCCAT
CGGACGGGTCCGGCCGGGCTCGGACTCCCTGCTGCGGATGCGGTTGGACCTGGCCGAATGCGCGACCGGCGTGACCAAGC
AGGTCACCGTCGACACCGCCGTGCTGTGCGACCTGTGCCACGGTAAGGGCACCCACGGCACCTCCACCCCCGCGACGTGC
GACACCTGCGGTGGCCGCGGTGAGATCCAGACCGTGCAGCGATCGCTGCTGGGCCAGGTCATGACGACGCGGCCGTGCCC
GGTGTGCGGCGGAGTCGGCGAAGTGATCCCCGATCCGTGCCACCGCTGCGGCGGCGACGGCCGCGTCCGCGCCCGGCGTG
AGATCAGCGTCAAGATCCCGGCCGGGGTCGGCGACGGCATGCGCGTGCGGCTGGCCGCCCAGGGTGAGGTCGGACCCGGC
GGGGGACCGGCGGGCGACCTGTACGTCGAGGTGCACGAGAAGCCGCACGACGTGTACGTCCGCGACGGCGACGACCTGCA
CTGCACGGTGTCGGTGCCGATGGTCGACGCCGCGCTGGGCACCACCGTCAGCGTCGACGACATCCTCGGGGGTCCGCTCG
AACTCACCATCGCCGCCGGTACCCAGCCGGGCGCGGTGTCGACGCTGCGCGGCCACGGCATGCCGCACCTGCGGTCGGGT
GTCCGGGGCGACCTGCACGCCCACATCGACGTGGTGGTGCCGGCCAAACTGGACCACACCGACATCGAGCTGCTGCGCCA
GCTCAAGGAGCATCGCAGCCGCGACGTCGCCGAGGTCCGCACCAGCAGTTCACCGGCCGCGTCGAACGCCGGCGGACTGT
TCAGCCGGCTGCGCGAGACGTTCACCGGCCGCTAG

Upstream 100 bases:

>100_bases
CGCTGCGGTTGCTTTGTACATAGGTGAGGTCCTGGGCACGCGATGACCGCGGCGCAGGGAGAGTCTCAACAGCCGGTACA
CCGGCACTGAAAGGTCAAGC

Downstream 100 bases:

>100_bases
GAAGGCTCGTCGCGTGCGCGACCTCTTCTACGTCGACGCGCTGCCCGGCCTCGGTGAGCTCGCCGTCGTCGACGGCGACG
CCGGTTTTCACGCCACCAAC

Product: chaperone protein DnaJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MARDYYGLLGVSKGASDQEIKRAYRKLARELHPDVNPDEEAQARFKEISAAYEVLSDPEKRRIVDLGGDPLESAGASGGN
GFGAGFGGLGDVFEAFFGGGGGSRGPIGRVRPGSDSLLRMRLDLAECATGVTKQVTVDTAVLCDLCHGKGTHGTSTPATC
DTCGGRGEIQTVQRSLLGQVMTTRPCPVCGGVGEVIPDPCHRCGGDGRVRARREISVKIPAGVGDGMRVRLAAQGEVGPG
GGPAGDLYVEVHEKPHDVYVRDGDDLHCTVSVPMVDAALGTTVSVDDILGGPLELTIAAGTQPGAVSTLRGHGMPHLRSG
VRGDLHAHIDVVVPAKLDHTDIELLRQLKEHRSRDVAEVRTSSSPAASNAGGLFSRLRETFTGR

Sequences:

>Translated_384_residues
MARDYYGLLGVSKGASDQEIKRAYRKLARELHPDVNPDEEAQARFKEISAAYEVLSDPEKRRIVDLGGDPLESAGASGGN
GFGAGFGGLGDVFEAFFGGGGGSRGPIGRVRPGSDSLLRMRLDLAECATGVTKQVTVDTAVLCDLCHGKGTHGTSTPATC
DTCGGRGEIQTVQRSLLGQVMTTRPCPVCGGVGEVIPDPCHRCGGDGRVRARREISVKIPAGVGDGMRVRLAAQGEVGPG
GGPAGDLYVEVHEKPHDVYVRDGDDLHCTVSVPMVDAALGTTVSVDDILGGPLELTIAAGTQPGAVSTLRGHGMPHLRSG
VRGDLHAHIDVVVPAKLDHTDIELLRQLKEHRSRDVAEVRTSSSPAASNAGGLFSRLRETFTGR
>Mature_383_residues
ARDYYGLLGVSKGASDQEIKRAYRKLARELHPDVNPDEEAQARFKEISAAYEVLSDPEKRRIVDLGGDPLESAGASGGNG
FGAGFGGLGDVFEAFFGGGGGSRGPIGRVRPGSDSLLRMRLDLAECATGVTKQVTVDTAVLCDLCHGKGTHGTSTPATCD
TCGGRGEIQTVQRSLLGQVMTTRPCPVCGGVGEVIPDPCHRCGGDGRVRARREISVKIPAGVGDGMRVRLAAQGEVGPGG
GPAGDLYVEVHEKPHDVYVRDGDDLHCTVSVPMVDAALGTTVSVDDILGGPLELTIAAGTQPGAVSTLRGHGMPHLRSGV
RGDLHAHIDVVVPAKLDHTDIELLRQLKEHRSRDVAEVRTSSSPAASNAGGLFSRLRETFTGR

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion. Unfolded proteins bind initially to dnaJ; upon

COG id: COG0484

COG function: function code O; DnaJ-class molecular chaperone with C-terminal Zn finger domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain [H]

Homologues:

Organism=Homo sapiens, GI194328758, Length=353, Percent_Identity=35.1274787535411, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI205360838, Length=404, Percent_Identity=30.9405940594059, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI194328760, Length=353, Percent_Identity=35.1274787535411, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI205360840, Length=354, Percent_Identity=32.4858757062147, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI5031741, Length=340, Percent_Identity=34.7058823529412, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4504511, Length=354, Percent_Identity=32.7683615819209, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI7706495, Length=341, Percent_Identity=31.6715542521994, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI194328762, Length=317, Percent_Identity=32.4921135646688, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI39204547, Length=355, Percent_Identity=26.4788732394366, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI6631085, Length=187, Percent_Identity=34.7593582887701, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI194306642, Length=104, Percent_Identity=48.0769230769231, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI194306640, Length=104, Percent_Identity=48.0769230769231, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI47777312, Length=153, Percent_Identity=40.5228758169935, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI5453690, Length=185, Percent_Identity=32.972972972973, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI27151736, Length=107, Percent_Identity=44.8598130841121, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI88501736, Length=107, Percent_Identity=44.8598130841121, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI23503241, Length=104, Percent_Identity=46.1538461538462, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI17388799, Length=104, Percent_Identity=49.0384615384615, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI201862587, Length=69, Percent_Identity=52.1739130434783, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI56549115, Length=106, Percent_Identity=41.5094339622642, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI201862518, Length=69, Percent_Identity=52.1739130434783, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI4885495, Length=104, Percent_Identity=49.0384615384615, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI72534740, Length=103, Percent_Identity=42.7184466019417, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI9558755, Length=114, Percent_Identity=39.4736842105263, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI21553335, Length=103, Percent_Identity=43.6893203883495, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI5453980, Length=104, Percent_Identity=46.1538461538462, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI56687498, Length=66, Percent_Identity=56.0606060606061, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI22749377, Length=102, Percent_Identity=42.156862745098, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI24308127, Length=68, Percent_Identity=50, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI21361912, Length=62, Percent_Identity=48.3870967741936, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI29126218, Length=79, Percent_Identity=48.1012658227848, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI68077168, Length=76, Percent_Identity=48.6842105263158, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI68077166, Length=76, Percent_Identity=48.6842105263158, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786197, Length=352, Percent_Identity=43.1818181818182, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1787235, Length=357, Percent_Identity=29.6918767507003, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17553096, Length=346, Percent_Identity=34.1040462427746, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17553098, Length=354, Percent_Identity=33.3333333333333, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17507263, Length=340, Percent_Identity=35, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17534355, Length=357, Percent_Identity=29.4117647058824, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI212645553, Length=357, Percent_Identity=31.6526610644258, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17563890, Length=231, Percent_Identity=27.2727272727273, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554644, Length=100, Percent_Identity=52, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17510335, Length=77, Percent_Identity=49.3506493506493, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17551190, Length=74, Percent_Identity=52.7027027027027, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115532476, Length=65, Percent_Identity=55.3846153846154, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115532378, Length=86, Percent_Identity=41.8604651162791, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17568643, Length=66, Percent_Identity=53.030303030303, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538236, Length=113, Percent_Identity=37.1681415929204, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17554900, Length=85, Percent_Identity=37.6470588235294, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324265, Length=352, Percent_Identity=36.3636363636364, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6323870, Length=357, Percent_Identity=32.7731092436975, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6323119, Length=372, Percent_Identity=28.7634408602151, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321094, Length=414, Percent_Identity=29.2270531400966, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324252, Length=422, Percent_Identity=25.3554502369668, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324103, Length=63, Percent_Identity=60.3174603174603, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323813, Length=108, Percent_Identity=42.5925925925926, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320888, Length=99, Percent_Identity=42.4242424242424, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324321, Length=69, Percent_Identity=52.1739130434783, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322194, Length=99, Percent_Identity=43.4343434343434, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45552813, Length=376, Percent_Identity=30.5851063829787, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45549272, Length=376, Percent_Identity=30.5851063829787, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45552811, Length=376, Percent_Identity=30.5851063829787, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646562, Length=353, Percent_Identity=33.1444759206799, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24646560, Length=353, Percent_Identity=33.1444759206799, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24646558, Length=353, Percent_Identity=33.1444759206799, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24646556, Length=353, Percent_Identity=33.1444759206799, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI21357547, Length=353, Percent_Identity=33.1444759206799, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI20129487, Length=338, Percent_Identity=32.8402366863905, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24584014, Length=338, Percent_Identity=32.8402366863905, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19920464, Length=355, Percent_Identity=30.7042253521127, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI85726398, Length=371, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24658562, Length=367, Percent_Identity=25.8855585831063, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24658555, Length=367, Percent_Identity=25.8855585831063, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18858081, Length=348, Percent_Identity=28.1609195402299, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28574949, Length=372, Percent_Identity=26.3440860215054, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24654066, Length=115, Percent_Identity=46.0869565217391, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24654070, Length=115, Percent_Identity=46.0869565217391, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24654068, Length=115, Percent_Identity=46.0869565217391, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24654072, Length=115, Percent_Identity=46.0869565217391, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI161077138, Length=64, Percent_Identity=62.5, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355911, Length=89, Percent_Identity=51.685393258427, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24668492, Length=69, Percent_Identity=44.9275362318841, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358273, Length=115, Percent_Identity=40, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17648033, Length=109, Percent_Identity=37.6146788990826, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584630, Length=109, Percent_Identity=37.6146788990826, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012724
- InterPro:   IPR002939
- InterPro:   IPR001623
- InterPro:   IPR008971
- InterPro:   IPR015609
- InterPro:   IPR003095
- InterPro:   IPR001305 [H]

Pfam domain/function: PF00226 DnaJ; PF01556 DnaJ_C; PF00684 DnaJ_CXXCXGXG [H]

EC number: NA

Molecular weight: Translated: 40196; Mature: 40065

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50076 DNAJ_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDYYGLLGVSKGASDQEIKRAYRKLARELHPDVNPDEEAQARFKEISAAYEVLSDPEK
CCCCCEEHEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
RRIVDLGGDPLESAGASGGNGFGAGFGGLGDVFEAFFGGGGGSRGPIGRVRPGSDSLLRM
HEEEECCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
RLDLAECATGVTKQVTVDTAVLCDLCHGKGTHGTSTPATCDTCGGRGEIQTVQRSLLGQV
HHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
MTTRPCPVCGGVGEVIPDPCHRCGGDGRVRARREISVKIPAGVGDGMRVRLAAQGEVGPG
HHCCCCCCCCCCHHCCCCHHHHCCCCCCEEEEEEEEEEECCCCCCCCEEEEEECCCCCCC
GGPAGDLYVEVHEKPHDVYVRDGDDLHCTVSVPMVDAALGTTVSVDDILGGPLELTIAAG
CCCCCCEEEEEECCCCEEEEECCCCEEEEEECCCHHHHHCCEEEHHHHCCCCEEEEEECC
TQPGAVSTLRGHGMPHLRSGVRGDLHAHIDVVVPAKLDHTDIELLRQLKEHRSRDVAEVR
CCCCCCHHHHCCCCHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHH
TSSSPAASNAGGLFSRLRETFTGR
CCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARDYYGLLGVSKGASDQEIKRAYRKLARELHPDVNPDEEAQARFKEISAAYEVLSDPEK
CCCCEEHEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
RRIVDLGGDPLESAGASGGNGFGAGFGGLGDVFEAFFGGGGGSRGPIGRVRPGSDSLLRM
HEEEECCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
RLDLAECATGVTKQVTVDTAVLCDLCHGKGTHGTSTPATCDTCGGRGEIQTVQRSLLGQV
HHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
MTTRPCPVCGGVGEVIPDPCHRCGGDGRVRARREISVKIPAGVGDGMRVRLAAQGEVGPG
HHCCCCCCCCCCHHCCCCHHHHCCCCCCEEEEEEEEEEECCCCCCCCEEEEEECCCCCCC
GGPAGDLYVEVHEKPHDVYVRDGDDLHCTVSVPMVDAALGTTVSVDDILGGPLELTIAAG
CCCCCCEEEEEECCCCEEEEECCCCEEEEEECCCHHHHHCCEEEHHHHCCCCEEEEEECC
TQPGAVSTLRGHGMPHLRSGVRGDLHAHIDVVVPAKLDHTDIELLRQLKEHRSRDVAEVR
CCCCCCHHHHCCCCHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHH
TSSSPAASNAGGLFSRLRETFTGR
CCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]