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The map label for this gene is cobD [H]

Identifier: 108800315

GI number: 108800315

Start: 3547960

End: 3548994

Strand: Reverse

Name: cobD [H]

Synonym: Mmcs_3349

Alternate gene names: 108800315

Gene position: 3548994-3547960 (Counterclockwise)

Preceding gene: 108800321

Following gene: 108800311

Centisome position: 62.2

GC content: 74.59

Gene sequence:

>1035_bases
ATGGTTTCGCCGATCATGCGTGCCCGCGCCGCCGGAATCGCCGCCGGACTGGTCGCCGATGCGCTGCTGGGTGATCCGCG
CCGCGGACATCCGGTCGCCGGATTCGGCCGCGCCGCAGCGCAATTGGAGCGCATCAGCTACGCCGACTCCCGACGGGCGG
GCGCACTGCACACCGGTGCGCTGCTGAGCGCGCTCGCCGTCGCCGGCGCGACGGCGCAGAGGGCGGCGCGTCGCAGCCCC
TGGGCGACGACCGCACTCACCGCGGCTGCCACCTTCGTCGCGGCGGGCGGGACCTCGCTGTGCCGGGTGGGTGACCGACT
GTCGGAGGTGTTGGACACCGGTGACATCGAGGCCGGCCGCGATCTGCTGCCGTCACTGTGCGGACGGGATCCGTCGGTGC
TCGACGCCGGGGGCCTGACCCGCGCGGCCGTGGAGTCGATCGCCGAGAACACCTCCGACGCCCAGGTTGCGCCGCTGGTG
TGGGCGGCCGTCGCCGGTGTGCCCGGTCTGCTGGTGTACCGGGGAGCGAACACCCTCGACGCTATGATCGGCAACCGCTC
CCCGAGATATGCCCGATTCGGCTGGGCCGCAGCACGACTCGATGACGCCCTCAACTACCTGCCGGCTCGTCTGACCGGGC
TGCTGGCGGCGCTGTGCGCGCCGGTGGTCGGTGGTTCACCCGTCGCCGCGATGCACGCCTGGCGGCGCGACGCCCACCGG
CACCCCAGCCCCAACGCCGGGGTCGCCGAGGCGACGTTCGCCGGTGCGCTGGGCGTGCGTCTGGGTGGGCCCACCCAGTA
CGCCCACGAACTGGAGATCAGGCCGACGCTGGGGGACGGACGCGCTCCCGTGCCCGCCGATCTGCGGCGGGCGGTGCGGC
TGTCGCGGGTGGTGCAGGGCGCGGCCGCGGTGCTGGCGGTGGCGGTCAGCGCCGCCGGCCGTAGCGGTCGGCCAGCTTGT
CCTCGGGGGTCGCGGGCGCGGTGGAGTCCGCGGCAGCCTTGTCGCGGCGGCGCTGACGGACCTCGGCGTAGATGA

Upstream 100 bases:

>100_bases
ATTGACCACATCGCAAATTCCGATGCTCATCGGTGATCTTGTCAAATTTGCCAAGCTGGGATGCCGTCGACTGCGCATCA
TCGTGTGAGACGCTCCCGGG

Downstream 100 bases:

>100_bases
AATAGCCGAGGCCGATCGGGGCGATGATGCCGAACGCGATCCACTGGATCCCGTAGGACAGGAACGGGCCGGCATCGAGG
TGCGGTAGACCGATGACGCC

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MVSPIMRARAAGIAAGLVADALLGDPRRGHPVAGFGRAAAQLERISYADSRRAGALHTGALLSALAVAGATAQRAARRSP
WATTALTAAATFVAAGGTSLCRVGDRLSEVLDTGDIEAGRDLLPSLCGRDPSVLDAGGLTRAAVESIAENTSDAQVAPLV
WAAVAGVPGLLVYRGANTLDAMIGNRSPRYARFGWAAARLDDALNYLPARLTGLLAALCAPVVGGSPVAAMHAWRRDAHR
HPSPNAGVAEATFAGALGVRLGGPTQYAHELEIRPTLGDGRAPVPADLRRAVRLSRVVQGAAAVLAVAVSAAGRSGRPAC
PRGSRARWSPRQPCRGGADGPRRR

Sequences:

>Translated_344_residues
MVSPIMRARAAGIAAGLVADALLGDPRRGHPVAGFGRAAAQLERISYADSRRAGALHTGALLSALAVAGATAQRAARRSP
WATTALTAAATFVAAGGTSLCRVGDRLSEVLDTGDIEAGRDLLPSLCGRDPSVLDAGGLTRAAVESIAENTSDAQVAPLV
WAAVAGVPGLLVYRGANTLDAMIGNRSPRYARFGWAAARLDDALNYLPARLTGLLAALCAPVVGGSPVAAMHAWRRDAHR
HPSPNAGVAEATFAGALGVRLGGPTQYAHELEIRPTLGDGRAPVPADLRRAVRLSRVVQGAAAVLAVAVSAAGRSGRPAC
PRGSRARWSPRQPCRGGADGPRRR
>Mature_344_residues
MVSPIMRARAAGIAAGLVADALLGDPRRGHPVAGFGRAAAQLERISYADSRRAGALHTGALLSALAVAGATAQRAARRSP
WATTALTAAATFVAAGGTSLCRVGDRLSEVLDTGDIEAGRDLLPSLCGRDPSVLDAGGLTRAAVESIAENTSDAQVAPLV
WAAVAGVPGLLVYRGANTLDAMIGNRSPRYARFGWAAARLDDALNYLPARLTGLLAALCAPVVGGSPVAAMHAWRRDAHR
HPSPNAGVAEATFAGALGVRLGGPTQYAHELEIRPTLGDGRAPVPADLRRAVRLSRVVQGAAAVLAVAVSAAGRSGRPAC
PRGSRARWSPRQPCRGGADGPRRR

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 35255; Mature: 35255

Theoretical pI: Translated: 11.73; Mature: 11.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSPIMRARAAGIAAGLVADALLGDPRRGHPVAGFGRAAAQLERISYADSRRAGALHTGA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
LLSALAVAGATAQRAARRSPWATTALTAAATFVAAGGTSLCRVGDRLSEVLDTGDIEAGR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHH
DLLPSLCGRDPSVLDAGGLTRAAVESIAENTSDAQVAPLVWAAVAGVPGLLVYRGANTLD
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHH
AMIGNRSPRYARFGWAAARLDDALNYLPARLTGLLAALCAPVVGGSPVAAMHAWRRDAHR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
HPSPNAGVAEATFAGALGVRLGGPTQYAHELEIRPTLGDGRAPVPADLRRAVRLSRVVQG
CCCCCCCCHHHHHHHHHHEECCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
AAAVLAVAVSAAGRSGRPACPRGSRARWSPRQPCRGGADGPRRR
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVSPIMRARAAGIAAGLVADALLGDPRRGHPVAGFGRAAAQLERISYADSRRAGALHTGA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
LLSALAVAGATAQRAARRSPWATTALTAAATFVAAGGTSLCRVGDRLSEVLDTGDIEAGR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHH
DLLPSLCGRDPSVLDAGGLTRAAVESIAENTSDAQVAPLVWAAVAGVPGLLVYRGANTLD
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHH
AMIGNRSPRYARFGWAAARLDDALNYLPARLTGLLAALCAPVVGGSPVAAMHAWRRDAHR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
HPSPNAGVAEATFAGALGVRLGGPTQYAHELEIRPTLGDGRAPVPADLRRAVRLSRVVQG
CCCCCCCCHHHHHHHHHHEECCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
AAAVLAVAVSAAGRSGRPACPRGSRARWSPRQPCRGGADGPRRR
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]