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The map label for this gene is ilvE [H]

Identifier: 108800274

GI number: 108800274

Start: 3504023

End: 3505129

Strand: Reverse

Name: ilvE [H]

Synonym: Mmcs_3308

Alternate gene names: 108800274

Gene position: 3505129-3504023 (Counterclockwise)

Preceding gene: 108800275

Following gene: 108800268

Centisome position: 61.43

GC content: 68.2

Gene sequence:

>1107_bases
ATGACTGACGGCCCCCTCGAGTTCACCGTTCACCGCAACGCGGAGCCGGCGACCGACGAGGTACGCGCCGAGATCCTGGC
CAACCCCGGCTTCGGCCGCTTCCACACCGACCACATGGTGTCGATCGACTACACCGCCGACGCCGGCTGGCACAACGCAC
AGGTGCTGCCCTACGGCCCGATCGAACTCGACCCGTCGGCCGTTGTGCTGCACTACGCGCAGGAGATCTTCGAGGGACTC
AAGGCCTACCGGTGGGCGGACGGGTCGGTGGTGTCGTTCCGTCCCGAGTCCAACGCCGCACGGCTGCGGTCCTCGGCGCG
CCGGCTGGCCATTCCGGAGTTGCCGGAGGAGCTGTTCCTCGAATCGCTGCGCCAGCTCATCGCGATCGACGAACAGTGGG
TGCCGCCCGCCGGGGGTGAGGAGTCGCTGTACTTGCGGCCGTTCGTCATCGCGACCGAACCGGGTCTGGGCGTCCGGCCC
GCCAACGAGTACCGCTACCTGGTGATCGGCTCGCCGGCCGGCGCGTACTTCCCGCGCGGGATCAAACCGGTCAGCGTGTG
GTTGTCGCACGAGTACGTGCGGGCCAGCCCCGGCGGCACCGGCGCGGCGAAGTTCGGCGGCAACTACGCGGCCTCGCTGC
TCGCGCAGGCGCAGGCCACCGAACACGGCTGCGATCAGGTGGTGTGGCTCGATGCGATCGAACGCCGCTACGTCGAGGAG
ATGGGCGGGATGAACCTGTTCTTCGTCTTCGGCAGCGGGGGTTCGGCGCGGTTGGTGACACCGGAGCTGAGCGGTTCACT
ACTGCCGGGCATCACGCGAGACTCGTTGCTGCAGTTGGCGACCGACGCCGGATTCGCGGTCGAGGAACGCAAGATCGACG
TCGACGAGTGGCAGAAGAAGGCCGCCGCCGGGGAGATCAGCGAGGTGTTCGCGTGTGGCACCGCAGCGGTCATCACACCG
GTCAGCCACGTCAAGCACCACGACGGCGAGTTCACCATCGCCGACGGGGAACCCGGCGAGGTGACGATGGCGCTGCGCGA
CACCCTCACCGGCATCCAGCGCGGCAAGTTCGCCGACACCCACGGCTGGATGGTCAAGCTGAACTGA

Upstream 100 bases:

>100_bases
TGGAGGTCGACGTGCGTGGCCGGCGCATCGAGTGCGAGGTCGTGCCTCCGCCGTTTGTCACCGCGAAAACCCGCTAGCCA
CCGGAGATACAATTTCTTCC

Downstream 100 bases:

>100_bases
CGCCGCCGGCTCAGGGGGCGATGCGCACAGGCGGGTCGGCGAAGGCGGGATCCGCCGGCTGCTGACCGGGGCCCATCAGG
CCGCAGTGCTTGACGGGCAC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTDGPLEFTVHRNAEPATDEVRAEILANPGFGRFHTDHMVSIDYTADAGWHNAQVLPYGPIELDPSAVVLHYAQEIFEGL
KAYRWADGSVVSFRPESNAARLRSSARRLAIPELPEELFLESLRQLIAIDEQWVPPAGGEESLYLRPFVIATEPGLGVRP
ANEYRYLVIGSPAGAYFPRGIKPVSVWLSHEYVRASPGGTGAAKFGGNYAASLLAQAQATEHGCDQVVWLDAIERRYVEE
MGGMNLFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLATDAGFAVEERKIDVDEWQKKAAAGEISEVFACGTAAVITP
VSHVKHHDGEFTIADGEPGEVTMALRDTLTGIQRGKFADTHGWMVKLN

Sequences:

>Translated_368_residues
MTDGPLEFTVHRNAEPATDEVRAEILANPGFGRFHTDHMVSIDYTADAGWHNAQVLPYGPIELDPSAVVLHYAQEIFEGL
KAYRWADGSVVSFRPESNAARLRSSARRLAIPELPEELFLESLRQLIAIDEQWVPPAGGEESLYLRPFVIATEPGLGVRP
ANEYRYLVIGSPAGAYFPRGIKPVSVWLSHEYVRASPGGTGAAKFGGNYAASLLAQAQATEHGCDQVVWLDAIERRYVEE
MGGMNLFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLATDAGFAVEERKIDVDEWQKKAAAGEISEVFACGTAAVITP
VSHVKHHDGEFTIADGEPGEVTMALRDTLTGIQRGKFADTHGWMVKLN
>Mature_367_residues
TDGPLEFTVHRNAEPATDEVRAEILANPGFGRFHTDHMVSIDYTADAGWHNAQVLPYGPIELDPSAVVLHYAQEIFEGLK
AYRWADGSVVSFRPESNAARLRSSARRLAIPELPEELFLESLRQLIAIDEQWVPPAGGEESLYLRPFVIATEPGLGVRPA
NEYRYLVIGSPAGAYFPRGIKPVSVWLSHEYVRASPGGTGAAKFGGNYAASLLAQAQATEHGCDQVVWLDAIERRYVEEM
GGMNLFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLATDAGFAVEERKIDVDEWQKKAAAGEISEVFACGTAAVITPV
SHVKHHDGEFTIADGEPGEVTMALRDTLTGIQRGKFADTHGWMVKLN

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=345, Percent_Identity=39.1304347826087, Blast_Score=244, Evalue=6e-65,
Organism=Homo sapiens, GI296010904, Length=345, Percent_Identity=39.1304347826087, Blast_Score=244, Evalue=7e-65,
Organism=Homo sapiens, GI296010906, Length=345, Percent_Identity=39.1304347826087, Blast_Score=244, Evalue=7e-65,
Organism=Homo sapiens, GI50658084, Length=338, Percent_Identity=39.3491124260355, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI296010902, Length=345, Percent_Identity=34.7826086956522, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI296010900, Length=345, Percent_Identity=34.7826086956522, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI258614015, Length=294, Percent_Identity=40.1360544217687, Blast_Score=198, Evalue=6e-51,
Organism=Escherichia coli, GI48994963, Length=334, Percent_Identity=33.2335329341317, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17568601, Length=344, Percent_Identity=38.953488372093, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17565728, Length=343, Percent_Identity=35.5685131195335, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6322002, Length=367, Percent_Identity=38.6920980926431, Blast_Score=236, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6322608, Length=336, Percent_Identity=38.6904761904762, Blast_Score=225, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24641779, Length=344, Percent_Identity=39.5348837209302, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 40047; Mature: 39916

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGPLEFTVHRNAEPATDEVRAEILANPGFGRFHTDHMVSIDYTADAGWHNAQVLPYGP
CCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEECCEEEEEEEECCCCCCCCEEEECCC
IELDPSAVVLHYAQEIFEGLKAYRWADGSVVSFRPESNAARLRSSARRLAIPELPEELFL
CEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHH
ESLRQLIAIDEQWVPPAGGEESLYLRPFVIATEPGLGVRPANEYRYLVIGSPAGAYFPRG
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCC
IKPVSVWLSHEYVRASPGGTGAAKFGGNYAASLLAQAQATEHGCDQVVWLDAIERRYVEE
CCEEEEEEECCEEEECCCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
MGGMNLFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLATDAGFAVEERKIDVDEWQKK
HCCCEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHH
AAAGEISEVFACGTAAVITPVSHVKHHDGEFTIADGEPGEVTMALRDTLTGIQRGKFADT
HHCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEHHHHHHHHHHCCCEECC
HGWMVKLN
CCEEEEEC
>Mature Secondary Structure 
TDGPLEFTVHRNAEPATDEVRAEILANPGFGRFHTDHMVSIDYTADAGWHNAQVLPYGP
CCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEECCEEEEEEEECCCCCCCCEEEECCC
IELDPSAVVLHYAQEIFEGLKAYRWADGSVVSFRPESNAARLRSSARRLAIPELPEELFL
CEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHH
ESLRQLIAIDEQWVPPAGGEESLYLRPFVIATEPGLGVRPANEYRYLVIGSPAGAYFPRG
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCC
IKPVSVWLSHEYVRASPGGTGAAKFGGNYAASLLAQAQATEHGCDQVVWLDAIERRYVEE
CCEEEEEEECCEEEECCCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
MGGMNLFFVFGSGGSARLVTPELSGSLLPGITRDSLLQLATDAGFAVEERKIDVDEWQKK
HCCCEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHH
AAAGEISEVFACGTAAVITPVSHVKHHDGEFTIADGEPGEVTMALRDTLTGIQRGKFADT
HHCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEHHHHHHHHHHCCCEECC
HGWMVKLN
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA