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The map label for this gene is pyk [H]

Identifier: 108800008

GI number: 108800008

Start: 3213983

End: 3215401

Strand: Reverse

Name: pyk [H]

Synonym: Mmcs_3042

Alternate gene names: 108800008

Gene position: 3215401-3213983 (Counterclockwise)

Preceding gene: 108800009

Following gene: 108800007

Centisome position: 56.36

GC content: 68.57

Gene sequence:

>1419_bases
GTGAATAGACGCGGGAAGATCGTCTGTACCCTCGGTCCGGCCACCGGCTCCGACGACGCCGTCCGGAAGCTGGTGGAGGC
CGGGATGGATGTCGCGCGGCTCAACTTCAGCCACGGCGACTACCCCGATCACGAAGCCAACTACAAGCGGGTCCGCGCGG
CCTCCGACCACACCGGACGCGCCGTCGGCATCCTCGCCGACCTGCAGGGTCCGAAGATCCGGCTGGGCCGCTTCGCCGAC
GGCCCGACCGTATGGGCCGAGGGCGAGACCGTCCGGATCACCGTCGACGACGTCGAGGGCACCCACGACCGCGTGTCGAC
CACCTACAAACGGTTGGCAGAGGACGCCACCCCCGGCGACCGGGTCCTCGTCGACGACGGCAACGTCGCGCTGGTGGTCA
ACGCCATCGAGGGCAACGACGTCGTCTGCGAGGTCACCGAGGGTGGCCGGGTCAGCAACAACAAGGGCATGTCGCTGCCC
GGGATGAACGTCTCGGCGCCCGCGCTGTCGGAGAAGGACATCGAGGACCTCGAGTTCGCGGTGCGCCTCGGTGTCGACCT
GGTCGCGCTGTCGTTCGTGCGGTCCCCGGCCGACGTCGAACTCGTGCACGAGGTGATGGACCGCGTGGGGCGGCGGGTCC
CGGTGATCGCCAAACTGGAGAAACCGGAGGCGATCGACAACCTCGAGGCGATCGTGCTGGCTTTCGACGCGATCATGGTC
GCCCGTGGCGATCTCGGCGTGGAACTGCCGCTCGAAGAGGTCCCGCTGGTGCAGAAGCGCGCCATCCAGATGGCCCGCGA
GAACGCCAAACCCGTCATCGTCGCCACGCAGATGCTGGAGTCGATGATCGAAAACTCCCGGCCCACCCGCGCCGAGGCAT
CCGACGTCGCCAACGCGGTGCTCGACGGCGCGGACGCGGTCATGCTCTCGGGTGAGACATCGGTGGGCAAGTATCCGCTC
GAGGCCGTGCGGACGATGGCGCGCATCATCATCGCCGTCGAGGAGAACTCCGTCGTAGTCCCGCCGCTGACCCACGTCCC
GCGCACCAAGCGCGGCGTGATCTCCTACGCCGCCCGCGACATCGGGGAGCGGTTGGACGCCAAGGCGCTCGTCGCGTTCA
CCCAGTCCGGGGACACCGTGCGGCGTCTCGCGCGGCTGCACACGCCGCTGCCCGTGCTCGCGTTCACCGCGCTGCCCGAG
GTGCGCAGCCAACTGGCCTTGACCTGGGGCACCGAAACGTTCATCGTGCCGCAGATCGGCACCACCGACGGCATGATCCG
CCAGGTCGACAAGTCGCTGCTCGAACTCGGCCGCTACAAGCGGGGCGATCTGGTGGTCATCGTCGCGGGTGCGCCGCCGG
GCACAGTAGGCTCCACGAATCTGATCCACGTGCACCGGATCGGGGAGGACGACGTCTAG

Upstream 100 bases:

>100_bases
CGCGTATCTGATGGGGGAGTCCGACCTGCCGGCGCCGGTGCGACCCGGCGCGCTGCCACTGGCCGTCGTCTGAATCGATC
AACGACTACGATGAGTCAAC

Downstream 100 bases:

>100_bases
ATCCTGAGGGGGCATCTTGTCGGCAGCGGATTTCGACGAGCTGCTGGCACTGCTCGACCTCCGCAGGGTCGACGACGACA
CCTTCGCCGGATCGCACCCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNRRGKIVCTLGPATGSDDAVRKLVEAGMDVARLNFSHGDYPDHEANYKRVRAASDHTGRAVGILADLQGPKIRLGRFAD
GPTVWAEGETVRITVDDVEGTHDRVSTTYKRLAEDATPGDRVLVDDGNVALVVNAIEGNDVVCEVTEGGRVSNNKGMSLP
GMNVSAPALSEKDIEDLEFAVRLGVDLVALSFVRSPADVELVHEVMDRVGRRVPVIAKLEKPEAIDNLEAIVLAFDAIMV
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLESMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPL
EAVRTMARIIIAVEENSVVVPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPVLAFTALPE
VRSQLALTWGTETFIVPQIGTTDGMIRQVDKSLLELGRYKRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV

Sequences:

>Translated_472_residues
MNRRGKIVCTLGPATGSDDAVRKLVEAGMDVARLNFSHGDYPDHEANYKRVRAASDHTGRAVGILADLQGPKIRLGRFAD
GPTVWAEGETVRITVDDVEGTHDRVSTTYKRLAEDATPGDRVLVDDGNVALVVNAIEGNDVVCEVTEGGRVSNNKGMSLP
GMNVSAPALSEKDIEDLEFAVRLGVDLVALSFVRSPADVELVHEVMDRVGRRVPVIAKLEKPEAIDNLEAIVLAFDAIMV
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLESMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPL
EAVRTMARIIIAVEENSVVVPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPVLAFTALPE
VRSQLALTWGTETFIVPQIGTTDGMIRQVDKSLLELGRYKRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV
>Mature_472_residues
MNRRGKIVCTLGPATGSDDAVRKLVEAGMDVARLNFSHGDYPDHEANYKRVRAASDHTGRAVGILADLQGPKIRLGRFAD
GPTVWAEGETVRITVDDVEGTHDRVSTTYKRLAEDATPGDRVLVDDGNVALVVNAIEGNDVVCEVTEGGRVSNNKGMSLP
GMNVSAPALSEKDIEDLEFAVRLGVDLVALSFVRSPADVELVHEVMDRVGRRVPVIAKLEKPEAIDNLEAIVLAFDAIMV
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLESMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPL
EAVRTMARIIIAVEENSVVVPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPVLAFTALPE
VRSQLALTWGTETFIVPQIGTTDGMIRQVDKSLLELGRYKRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=494, Percent_Identity=37.8542510121458, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=37.5510204081633, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=37.5510204081633, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=37.8542510121458, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=37.8542510121458, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=38.6759581881533, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=38.6759581881533, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=36.1702127659575, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=36.1702127659575, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=35.981308411215, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=42.9175475687104, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=39.7058823529412, Blast_Score=295, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=36.4583333333333, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=37.9668049792531, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=37.9668049792531, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17506829, Length=340, Percent_Identity=44.7058823529412, Blast_Score=278, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=340, Percent_Identity=44.7058823529412, Blast_Score=277, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=35.4639175257732, Blast_Score=267, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6324923, Length=488, Percent_Identity=33.8114754098361, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24648964, Length=497, Percent_Identity=37.4245472837022, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI28571814, Length=497, Percent_Identity=37.4245472837022, Blast_Score=294, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24648966, Length=460, Percent_Identity=34.7826086956522, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=28.7755102040816, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24646914, Length=269, Percent_Identity=34.2007434944238, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50802; Mature: 50802

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRGKIVCTLGPATGSDDAVRKLVEAGMDVARLNFSHGDYPDHEANYKRVRAASDHTGR
CCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCCC
AVGILADLQGPKIRLGRFADGPTVWAEGETVRITVDDVEGTHDRVSTTYKRLAEDATPGD
EEEEEEECCCCCEEECEECCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
RVLVDDGNVALVVNAIEGNDVVCEVTEGGRVSNNKGMSLPGMNVSAPALSEKDIEDLEFA
EEEEECCCEEEEEEEECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VRLGVDLVALSFVRSPADVELVHEVMDRVGRRVPVIAKLEKPEAIDNLEAIVLAFDAIMV
HHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHE
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLESMIENSRPTRAEASDVANAV
ECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADAVMLSGETSVGKYPLEAVRTMARIIIAVEENSVVVPPLTHVPRTKRGVISYAARD
HCCCCEEEEECCCCCCCCCHHHHHHHHHEEEEECCCCEEECCCCCCCCCHHHHHHHHHHH
IGERLDAKALVAFTQSGDTVRRLARLHTPLPVLAFTALPEVRSQLALTWGTETFIVPQIG
HHHHCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHCHHHHHHEEEEECCCEEEEECCC
TTDGMIRQVDKSLLELGRYKRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV
CCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MNRRGKIVCTLGPATGSDDAVRKLVEAGMDVARLNFSHGDYPDHEANYKRVRAASDHTGR
CCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCCC
AVGILADLQGPKIRLGRFADGPTVWAEGETVRITVDDVEGTHDRVSTTYKRLAEDATPGD
EEEEEEECCCCCEEECEECCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
RVLVDDGNVALVVNAIEGNDVVCEVTEGGRVSNNKGMSLPGMNVSAPALSEKDIEDLEFA
EEEEECCCEEEEEEEECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VRLGVDLVALSFVRSPADVELVHEVMDRVGRRVPVIAKLEKPEAIDNLEAIVLAFDAIMV
HHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHE
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLESMIENSRPTRAEASDVANAV
ECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADAVMLSGETSVGKYPLEAVRTMARIIIAVEENSVVVPPLTHVPRTKRGVISYAARD
HCCCCEEEEECCCCCCCCCHHHHHHHHHEEEEECCCCEEECCCCCCCCCHHHHHHHHHHH
IGERLDAKALVAFTQSGDTVRRLARLHTPLPVLAFTALPEVRSQLALTWGTETFIVPQIG
HHHHCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHCHHHHHHEEEEECCCEEEEECCC
TTDGMIRQVDKSLLELGRYKRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV
CCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA