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The map label for this gene is glnH [H]

Identifier: 108800000

GI number: 108800000

Start: 3205100

End: 3206035

Strand: Reverse

Name: glnH [H]

Synonym: Mmcs_3034

Alternate gene names: 108800000

Gene position: 3206035-3205100 (Counterclockwise)

Preceding gene: 108800007

Following gene: 108799999

Centisome position: 56.19

GC content: 65.28

Gene sequence:

>936_bases
ATGCAAGAGAAAGAGGCAACAGTGTCACGATGCCAGATCCGTACGGGGAAGATGTGGCGTATCGCAGCGATCTTTGCTGT
CAGCGGCGCGATGGCGCTGTCCGGGTGCGCGCAGAACACCGACACCAGTGGTCCCGCGGAGGAGACGACGGCGGCGACCG
CGGAGAAGGTCGACGAGATCGCCAACACCCTGCCGCAGCCGATCAAGGACCGCGGTTCGCTCATCGTCGGCGTCAACGTG
CCCTACGCCCCGAACGAGTTCAAGGATCCCAGCGGCAAGATCGTCGGCTTCGACGTCGACCTGATGAACGCGATCGCCTC
GACGCTCGGCGTGACGGCCGAATACCGCGAATCGGACTTCGAGAAGATCATCCCGTCCATCCAGGGCGGCACCTACGACG
TCGGCATGTCGTCGTTCACCGACACCAAGGAACGTGAGGCGTCGGTCGACTTCGTGACCTACTTCTCGGCGGGCATCCAG
TGGGCGCAGAAGGCGGGCGCCGGTATCGATCCCGCCAACGCCTGTGGCAAGCGGGTCGCCGTGCAGTCGACGACCATCGA
AGAGACCGAGGAACTCCCGGCCAAGAGCAAGGCCTGCACCGACGCCGGCAAGCCGGCCATCGAGATCGTCAAGTTCGACG
AGCAGGACGCGGTGGCCAACGCCGTTGTGCTCGGCCAGGCCGATGCGATGTCGGCGGACTCGCCGGTCACCGCCTACGCC
GTCAAGCAGAGCAACGGCAAGCTCGAGACGGCCGGCGAGATCTTCGACGCCGCGCCCTACGGGTGGCCCGTCGCCAAGGG
GTCGCCGCTGGGGCAGTCGCTGCAGCAGGCGCTCGAGCACCTCATCGAAACCGGCGCCTATGAACAGATCGCGAAGAACT
GGGGTGTCGAGGACGGGATGATCGACAAACCGGTGATCAACGGCGCGATCAGCTAG

Upstream 100 bases:

>100_bases
CCTGTGGCTTCGGTGCTGGGTCGTTAATGTTCCGTTACCGGAAGTGCGTGCTGCGACCGCGATGGTTAACGTCGTCGGTT
TGGAGACCGGGGACGGTCCG

Downstream 100 bases:

>100_bases
CGGAGTCGACCCAACACGATGACCGATGCCGATTCGTCGCCGCGCACCGACGCCGCTCCCATCGATGCCGTTCCCCTGCG
ACATCCCTGGCGGTGGGTGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MQEKEATVSRCQIRTGKMWRIAAIFAVSGAMALSGCAQNTDTSGPAEETTAATAEKVDEIANTLPQPIKDRGSLIVGVNV
PYAPNEFKDPSGKIVGFDVDLMNAIASTLGVTAEYRESDFEKIIPSIQGGTYDVGMSSFTDTKEREASVDFVTYFSAGIQ
WAQKAGAGIDPANACGKRVAVQSTTIEETEELPAKSKACTDAGKPAIEIVKFDEQDAVANAVVLGQADAMSADSPVTAYA
VKQSNGKLETAGEIFDAAPYGWPVAKGSPLGQSLQQALEHLIETGAYEQIAKNWGVEDGMIDKPVINGAIS

Sequences:

>Translated_311_residues
MQEKEATVSRCQIRTGKMWRIAAIFAVSGAMALSGCAQNTDTSGPAEETTAATAEKVDEIANTLPQPIKDRGSLIVGVNV
PYAPNEFKDPSGKIVGFDVDLMNAIASTLGVTAEYRESDFEKIIPSIQGGTYDVGMSSFTDTKEREASVDFVTYFSAGIQ
WAQKAGAGIDPANACGKRVAVQSTTIEETEELPAKSKACTDAGKPAIEIVKFDEQDAVANAVVLGQADAMSADSPVTAYA
VKQSNGKLETAGEIFDAAPYGWPVAKGSPLGQSLQQALEHLIETGAYEQIAKNWGVEDGMIDKPVINGAIS
>Mature_311_residues
MQEKEATVSRCQIRTGKMWRIAAIFAVSGAMALSGCAQNTDTSGPAEETTAATAEKVDEIANTLPQPIKDRGSLIVGVNV
PYAPNEFKDPSGKIVGFDVDLMNAIASTLGVTAEYRESDFEKIIPSIQGGTYDVGMSSFTDTKEREASVDFVTYFSAGIQ
WAQKAGAGIDPANACGKRVAVQSTTIEETEELPAKSKACTDAGKPAIEIVKFDEQDAVANAVVLGQADAMSADSPVTAYA
VKQSNGKLETAGEIFDAAPYGWPVAKGSPLGQSLQQALEHLIETGAYEQIAKNWGVEDGMIDKPVINGAIS

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=229, Percent_Identity=33.1877729257642, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788228, Length=237, Percent_Identity=26.5822784810127, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788648, Length=245, Percent_Identity=26.9387755102041, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787088, Length=220, Percent_Identity=29.0909090909091, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788649, Length=243, Percent_Identity=27.5720164609054, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 32779; Mature: 32779

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKEATVSRCQIRTGKMWRIAAIFAVSGAMALSGCAQNTDTSGPAEETTAATAEKVDEI
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
ANTLPQPIKDRGSLIVGVNVPYAPNEFKDPSGKIVGFDVDLMNAIASTLGVTAEYRESDF
HHHCCCCHHCCCCEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHH
EKIIPSIQGGTYDVGMSSFTDTKEREASVDFVTYFSAGIQWAQKAGAGIDPANACGKRVA
HHHHHHHCCCEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEE
VQSTTIEETEELPAKSKACTDAGKPAIEIVKFDEQDAVANAVVLGQADAMSADSPVTAYA
EECCCHHHHHHCCCCHHHHHCCCCCEEEEEEECCHHHHHHEEEECCCCCCCCCCCEEEEE
VKQSNGKLETAGEIFDAAPYGWPVAKGSPLGQSLQQALEHLIETGAYEQIAKNWGVEDGM
EECCCCCEEHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
IDKPVINGAIS
CCCHHHCCCCC
>Mature Secondary Structure
MQEKEATVSRCQIRTGKMWRIAAIFAVSGAMALSGCAQNTDTSGPAEETTAATAEKVDEI
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
ANTLPQPIKDRGSLIVGVNVPYAPNEFKDPSGKIVGFDVDLMNAIASTLGVTAEYRESDF
HHHCCCCHHCCCCEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHH
EKIIPSIQGGTYDVGMSSFTDTKEREASVDFVTYFSAGIQWAQKAGAGIDPANACGKRVA
HHHHHHHCCCEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEE
VQSTTIEETEELPAKSKACTDAGKPAIEIVKFDEQDAVANAVVLGQADAMSADSPVTAYA
EECCCHHHHHHCCCCHHHHHCCCCCEEEEEEECCHHHHHHEEEECCCCCCCCCCCEEEEE
VKQSNGKLETAGEIFDAAPYGWPVAKGSPLGQSLQQALEHLIETGAYEQIAKNWGVEDGM
EECCCCCEEHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
IDKPVINGAIS
CCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]