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The map label for this gene is sigF [H]

Identifier: 108799609

GI number: 108799609

Start: 2796849

End: 2797703

Strand: Reverse

Name: sigF [H]

Synonym: Mmcs_2642

Alternate gene names: 108799609

Gene position: 2797703-2796849 (Counterclockwise)

Preceding gene: 108799610

Following gene: 108799608

Centisome position: 49.04

GC content: 64.8

Gene sequence:

>855_bases
ATGACCGTCCACGCTCACCCGGAGGCGACTTCAACACCCGGCATCGATACGCCGTATGACGGCAACGACGTCGATGTCGA
TCGCCTTTTCGACCGTCTCGCTGAAAGCGCCGATGACAGCGAACGGGAGCGATGGCGTCGGCACATCATCACCGCGTGCG
TACCGATCGCCGACCACATCGCCTACCGATTCGTCGGGCGTGGTGAGCCGTCGGAGGATGTGATCCAGGTAGCCCGTATC
GGGTTGATCAAGACCGTCGATCGTTACGACCCGGACAAGGGCCATTTCCTGGCGTTCGCCGTCCCCACGATCCGAGGAGA
GATCCGACGGCACTTCCGGGACAACACCTGGACGGTGCGGGTGCCCCGCAAAGTGCAGGAGACCCAGCTGCGCATGCGCG
ACGCCGTCAACACGCTCTCGCAGCGACTCAACCGCACGCCCACGGGCGTCGAACTGGCCGACGAACTCGATGTCGGCAGC
GAGGAGCTCGGGGATTGCCAAGCCGCGACCACCGCTTATCGGCCGGTATCGCTCGACGCGCCGCTGCTCACCGCGCAGAA
CGACGGCGACACAGTGGGCTCGATGCAAGGAGGCGACGATCCGGGTTACGGCAGGGTGGAAGACCTCATCCTGCTTCAGG
AGGCCATCTCTGATCTCGACCCTCGGCGACGGGCGATCGTCGGGATGATCTTCTTCGACTGCCTCACGCAGCGCGAGGTG
GCCCGTCGGCTCCATGTCTCCCAGGTGCAGATCTCCCGCCTGCTCAACGATGCGTTGGCACGGATCCGTCAGCGGGTCAA
TCTGGACGCCGCGGCCGCACTGTGTGTCGTCGGTCCCTTCATCGGCACCGTCTGA

Upstream 100 bases:

>100_bases
TTCGCCCCGAGCAGGTTCCCGGCGGATGTGCATTTATTGCGTTTCGGCAACGATTGCGTGATCGAGCGTTTACGCATCCC
GGGGCGGGTAGGCGTGTTAC

Downstream 100 bases:

>100_bases
TGCGCCGCCCGGTCTCATCCCGCGCCCCCCGCGTGGCCGGTCGGGCAGGTGGACTCCGTGTGGTTTGACGACTGGGACGA
CCTCCTGCGGGTGCTGATCG

Product: RNA polymerase, sigma 28 subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTVHAHPEATSTPGIDTPYDGNDVDVDRLFDRLAESADDSERERWRRHIITACVPIADHIAYRFVGRGEPSEDVIQVARI
GLIKTVDRYDPDKGHFLAFAVPTIRGEIRRHFRDNTWTVRVPRKVQETQLRMRDAVNTLSQRLNRTPTGVELADELDVGS
EELGDCQAATTAYRPVSLDAPLLTAQNDGDTVGSMQGGDDPGYGRVEDLILLQEAISDLDPRRRAIVGMIFFDCLTQREV
ARRLHVSQVQISRLLNDALARIRQRVNLDAAAALCVVGPFIGTV

Sequences:

>Translated_284_residues
MTVHAHPEATSTPGIDTPYDGNDVDVDRLFDRLAESADDSERERWRRHIITACVPIADHIAYRFVGRGEPSEDVIQVARI
GLIKTVDRYDPDKGHFLAFAVPTIRGEIRRHFRDNTWTVRVPRKVQETQLRMRDAVNTLSQRLNRTPTGVELADELDVGS
EELGDCQAATTAYRPVSLDAPLLTAQNDGDTVGSMQGGDDPGYGRVEDLILLQEAISDLDPRRRAIVGMIFFDCLTQREV
ARRLHVSQVQISRLLNDALARIRQRVNLDAAAALCVVGPFIGTV
>Mature_283_residues
TVHAHPEATSTPGIDTPYDGNDVDVDRLFDRLAESADDSERERWRRHIITACVPIADHIAYRFVGRGEPSEDVIQVARIG
LIKTVDRYDPDKGHFLAFAVPTIRGEIRRHFRDNTWTVRVPRKVQETQLRMRDAVNTLSQRLNRTPTGVELADELDVGSE
ELGDCQAATTAYRPVSLDAPLLTAQNDGDTVGSMQGGDDPGYGRVEDLILLQEAISDLDPRRRAIVGMIFFDCLTQREVA
RRLHVSQVQISRLLNDALARIRQRVNLDAAAALCVVGPFIGTV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for normal spore maturation [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=232, Percent_Identity=26.2931034482759, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789098, Length=233, Percent_Identity=25.3218884120172, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789448, Length=148, Percent_Identity=27.027027027027, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 31662; Mature: 31531

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVHAHPEATSTPGIDTPYDGNDVDVDRLFDRLAESADDSERERWRRHIITACVPIADHI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AYRFVGRGEPSEDVIQVARIGLIKTVDRYDPDKGHFLAFAVPTIRGEIRRHFRDNTWTVR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCEEEE
VPRKVQETQLRMRDAVNTLSQRLNRTPTGVELADELDVGSEELGDCQAATTAYRPVSLDA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHCCHHHHHHHCCCCCCCC
PLLTAQNDGDTVGSMQGGDDPGYGRVEDLILLQEAISDLDPRRRAIVGMIFFDCLTQREV
CEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ARRLHVSQVQISRLLNDALARIRQRVNLDAAAALCVVGPFIGTV
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVHAHPEATSTPGIDTPYDGNDVDVDRLFDRLAESADDSERERWRRHIITACVPIADHI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AYRFVGRGEPSEDVIQVARIGLIKTVDRYDPDKGHFLAFAVPTIRGEIRRHFRDNTWTVR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCEEEE
VPRKVQETQLRMRDAVNTLSQRLNRTPTGVELADELDVGSEELGDCQAATTAYRPVSLDA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHCCHHHHHHHCCCCCCCC
PLLTAQNDGDTVGSMQGGDDPGYGRVEDLILLQEAISDLDPRRRAIVGMIFFDCLTQREV
CEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ARRLHVSQVQISRLLNDALARIRQRVNLDAAAALCVVGPFIGTV
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476207; 12000953 [H]