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The map label for this gene is gadA [H]

Identifier: 108798117

GI number: 108798117

Start: 1243608

End: 1244993

Strand: Direct

Name: gadA [H]

Synonym: Mmcs_1145

Alternate gene names: 108798117

Gene position: 1243608-1244993 (Clockwise)

Preceding gene: 108798116

Following gene: 108798118

Centisome position: 21.8

GC content: 65.95

Gene sequence:

>1386_bases
ATGTCGCGCACGCACAGTCGCGTCCAGCACGTCTCGCCCGCCTACACCGGCAGGTTGGCGATGGCGCCGGTGCCGTCACT
GCGGCTGCCCGACGAGGCGATGGAACCCCAGGCGGCCTACCGGTTCATCCACGACGAACTCATGCTCGACGGCAGCTCGC
GGCTCAACCTCGCCACCTTCGTCACCACGTGGATGGACCCCGAGGCCGAGAAGCTGATGGCCGAGACGTTCGACAAGAAC
ATGATCGACAAGGACGAGTACCCGGCGACCGCGGCGATCGAGCAGCGTTGCGTGTGCATGGTGGCCGACCTGTTCCACGC
CGAGGATCTGCGCGACGACGACCCGAACAGCGCGATCGGGGTGTCGACGATCGGGTCGAGCGAGGCGGTCATGCTCGCCG
GGCTGGCGATGAAATGGCGTTGGCGCCAGCGCCTCGGCGGCGATGCGTGGAAGGGCCGCACCCCGAATCTGGTGATGGGG
TCGAACGTCCAGGTGGTGTGGGAGAAGTTCTGCCGCTACTTCGACGTCGAACCGCGCTATCTGCCGATGGAGGACGGTCG
CTACGTCATCACTCCGGAGCAGGTGCTCGAGCACGTCGACGAGGACACCATCGGAGTGGTGGCGATCCTGGGCACCACCT
ACACCGGTGAACTGGAGCCCATCGGGGAGATCTGCGCCGCGCTCGACAAATTGGCCGCAGACGGGGGTGTCGACGTGCCC
GTCCACGTCGACGCCGCCAGCGGCGGGTTCGTGGTGCCGTTCCTGCATCCGGATCTGGTGTGGGACTTCCGGTTGCCGCG
GGTGGTGTCGATCAACGTCAGTGGACACAAGTACGGGCTCACCTATCCCGGCATCGGGTTCGTGGTGTGGCGCAACGCCG
AGCAGCTACCCGAGGAACTGGTGTTCCGGGTCAACTACCTCGGCGGCGACATGCCGACGTTCACGCTGAACTTCTCTCGT
CCCGGTAACCAGGTGGTCGGCCAGTATTACAACTTCCTGCGGTTGGGTCGCGGCGGCTACGCGCAGGTCATGCAGTCGCT
GTCGCAGACCGCCCGGTGGCTGGGCGACGAACTGCGCGACAGCGAGCATTTCGAGTTGATCACCGACGGTTCGGCGATCC
CGGTCGTGAGCTTCCGGTTGAAGGGCGACCCGGGCTACACCGAGTTCGACATCTCGCAGACGTTGCGCTCCTACGGCTGG
CAGGTGCCCGCGTACACCATGCCCGAGGGCGCCGAGGACGTCACGGTGCTCAGGGTCGTGGTACGCGAGGGGTTTTCCGC
CGATCTGGCCCGCGCGCTGCGCGACGACATGGTCACGGTGCTCAAGCATCTCGACGAGCTCAAGCCGGGTGGCCACTTCG
ATGAGGTGCAGCCGTTCGCCCATTAG

Upstream 100 bases:

>100_bases
CGGCCGACCCCGGCCCACGCCCGGCGCCCACCTCCGCGTCGCGCATCCTGCACCACATCCGCACGGCACTCGGCAGCATC
TGACCCCGAAAGGAAACCCA

Downstream 100 bases:

>100_bases
AAGGGCGGCCACAGGTAACCGGCGACGAGCGGTGCCCGCAGCTCATGGAACCCTGGGAGCGCGGAAGCGACCATCCGCGC
ACGGCAACGTACAGAGCCGG

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSRTHSRVQHVSPAYTGRLAMAPVPSLRLPDEAMEPQAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAEKLMAETFDKN
MIDKDEYPATAAIEQRCVCMVADLFHAEDLRDDDPNSAIGVSTIGSSEAVMLAGLAMKWRWRQRLGGDAWKGRTPNLVMG
SNVQVVWEKFCRYFDVEPRYLPMEDGRYVITPEQVLEHVDEDTIGVVAILGTTYTGELEPIGEICAALDKLAADGGVDVP
VHVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGIGFVVWRNAEQLPEELVFRVNYLGGDMPTFTLNFSR
PGNQVVGQYYNFLRLGRGGYAQVMQSLSQTARWLGDELRDSEHFELITDGSAIPVVSFRLKGDPGYTEFDISQTLRSYGW
QVPAYTMPEGAEDVTVLRVVVREGFSADLARALRDDMVTVLKHLDELKPGGHFDEVQPFAH

Sequences:

>Translated_461_residues
MSRTHSRVQHVSPAYTGRLAMAPVPSLRLPDEAMEPQAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAEKLMAETFDKN
MIDKDEYPATAAIEQRCVCMVADLFHAEDLRDDDPNSAIGVSTIGSSEAVMLAGLAMKWRWRQRLGGDAWKGRTPNLVMG
SNVQVVWEKFCRYFDVEPRYLPMEDGRYVITPEQVLEHVDEDTIGVVAILGTTYTGELEPIGEICAALDKLAADGGVDVP
VHVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGIGFVVWRNAEQLPEELVFRVNYLGGDMPTFTLNFSR
PGNQVVGQYYNFLRLGRGGYAQVMQSLSQTARWLGDELRDSEHFELITDGSAIPVVSFRLKGDPGYTEFDISQTLRSYGW
QVPAYTMPEGAEDVTVLRVVVREGFSADLARALRDDMVTVLKHLDELKPGGHFDEVQPFAH
>Mature_460_residues
SRTHSRVQHVSPAYTGRLAMAPVPSLRLPDEAMEPQAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAEKLMAETFDKNM
IDKDEYPATAAIEQRCVCMVADLFHAEDLRDDDPNSAIGVSTIGSSEAVMLAGLAMKWRWRQRLGGDAWKGRTPNLVMGS
NVQVVWEKFCRYFDVEPRYLPMEDGRYVITPEQVLEHVDEDTIGVVAILGTTYTGELEPIGEICAALDKLAADGGVDVPV
HVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGIGFVVWRNAEQLPEELVFRVNYLGGDMPTFTLNFSRP
GNQVVGQYYNFLRLGRGGYAQVMQSLSQTARWLGDELRDSEHFELITDGSAIPVVSFRLKGDPGYTEFDISQTLRSYGWQ
VPAYTMPEGAEDVTVLRVVVREGFSADLARALRDDMVTVLKHLDELKPGGHFDEVQPFAH

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=373, Percent_Identity=22.7882037533512, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1787769, Length=426, Percent_Identity=51.1737089201878, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1789934, Length=426, Percent_Identity=51.1737089201878, Blast_Score=431, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17557272, Length=351, Percent_Identity=23.9316239316239, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25148342, Length=353, Percent_Identity=27.4787535410765, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17543922, Length=384, Percent_Identity=24.21875, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323905, Length=465, Percent_Identity=40.4301075268817, Blast_Score=333, Evalue=4e-92,
Organism=Saccharomyces cerevisiae, GI6320500, Length=334, Percent_Identity=25.1497005988024, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355963, Length=402, Percent_Identity=24.3781094527363, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24654344, Length=402, Percent_Identity=24.3781094527363, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 51498; Mature: 51367

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTHSRVQHVSPAYTGRLAMAPVPSLRLPDEAMEPQAAYRFIHDELMLDGSSRLNLATF
CCCHHHHHHHCCCHHCCCEEECCCCCCCCCHHHCCHHHHHHHHHHHHEECCCCCCCCEEH
VTTWMDPEAEKLMAETFDKNMIDKDEYPATAAIEQRCVCMVADLFHAEDLRDDDPNSAIG
EEHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
VSTIGSSEAVMLAGLAMKWRWRQRLGGDAWKGRTPNLVMGSNVQVVWEKFCRYFDVEPRY
EEECCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCE
LPMEDGRYVITPEQVLEHVDEDTIGVVAILGTTYTGELEPIGEICAALDKLAADGGVDVP
EECCCCCEEECHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCE
VHVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGIGFVVWRNAEQLPEEL
EEEECCCCCEEEEECCCCEEEECCCCEEEEEEECCCEECCCCCCCEEEEECCHHHHHHHH
VFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGRGGYAQVMQSLSQTARWLGDELRD
HHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
SEHFELITDGSAIPVVSFRLKGDPGYTEFDISQTLRSYGWQVPAYTMPEGAEDVTVLRVV
CCCEEEEECCCCCEEEEEEECCCCCCCEECHHHHHHHHCCCCCCEECCCCCCHHHHHHHH
VREGFSADLARALRDDMVTVLKHLDELKPGGHFDEVQPFAH
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRTHSRVQHVSPAYTGRLAMAPVPSLRLPDEAMEPQAAYRFIHDELMLDGSSRLNLATF
CCHHHHHHHCCCHHCCCEEECCCCCCCCCHHHCCHHHHHHHHHHHHEECCCCCCCCEEH
VTTWMDPEAEKLMAETFDKNMIDKDEYPATAAIEQRCVCMVADLFHAEDLRDDDPNSAIG
EEHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
VSTIGSSEAVMLAGLAMKWRWRQRLGGDAWKGRTPNLVMGSNVQVVWEKFCRYFDVEPRY
EEECCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCE
LPMEDGRYVITPEQVLEHVDEDTIGVVAILGTTYTGELEPIGEICAALDKLAADGGVDVP
EECCCCCEEECHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCE
VHVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGIGFVVWRNAEQLPEEL
EEEECCCCCEEEEECCCCEEEECCCCEEEEEEECCCEECCCCCCCEEEEECCHHHHHHHH
VFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGRGGYAQVMQSLSQTARWLGDELRD
HHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
SEHFELITDGSAIPVVSFRLKGDPGYTEFDISQTLRSYGWQVPAYTMPEGAEDVTVLRVV
CCCEEEEECCCCCEEEEEEECCCCCCCEECHHHHHHHHCCCCCCEECCCCCCHHHHHHHH
VREGFSADLARALRDDMVTVLKHLDELKPGGHFDEVQPFAH
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]