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The map label for this gene is menD [H]

Identifier: 108797730

GI number: 108797730

Start: 796387

End: 798030

Strand: Direct

Name: menD [H]

Synonym: Mmcs_0751

Alternate gene names: 108797730

Gene position: 796387-798030 (Clockwise)

Preceding gene: 108797729

Following gene: 108797731

Centisome position: 13.96

GC content: 72.02

Gene sequence:

>1644_bases
ATGAACCCCTCGACGGCACAGGCGCGGGTCGTCGTCGACGAACTCGTTCGCGGCGGCGTGCACGACGTCGTGCTGTGCCC
GGGGTCGCGTAACGCACCGCTGGCCTTCGCGCTGGCCGACGCCGACCGCGCGGGCCGGCTGCGGCTGCACGTCCGCATCG
ACGAACGCACCGCCGGCTTCCTCGCGATCGGGTTGGCGGTCGCCGACCGGGCGCCGGTGTGCGTGGCGATGACGTCGGGC
ACCGCGGTCGCGAACCTGGGCCCCGCCGTCGTCGAGGCCAACTACGCTCGGGTGCCGCTGATCGTGCTCAGCGCGAACCG
GCCCTACGAACTGCTCGGCACCGGGGCGAACCAGACCTTCGAGCAGCTCGGCTACTTCGGCAACCAGGTACGGGCGAACA
TCAGCCTGGGTCTCGCCCCGGAATTGAGCTCAGGCAGTCCGGGCGATATGACTTCCCTCAACGCGCAGTGGCGTTCGGCG
ACCTGCCGGGTGGTGGTGGCGGCGACGGGTTCGCGCAGCGCCAACGCCGGCCCCGTGCAGTTCGACATCCCGCTGCGCGA
ACCGCTGGTGCCCACCTTCGACGACGACGGCTCCTGTCCGCCGGGCCGCCCCGACGGCAAACCGTGGACCCACACCCCGC
CGGTGACGTTCGACCAGCCGCTCGACATCGACCTCACCCCCGACACCGTGGTGATCGCCGGTCACGGCGCGGGCGTGCAC
CCGAACCTCGCCGACCTGCCCACCGTCGCCGAACCGACGGCACCGCCTGCGGCCAATCCGCTGCACCCGATGGCGCTGCG
CCTGCTGCGGCCCAAGCAGGTCATCATGCTCGGCCGCCCGACACTGCACCGGCCGGTCTCCGCGCTGCTCGCCGACCCGT
CGGTGCCGGTGTACGCGCTCACCACCGGGCCGCGCTGGCCCGACGTCAGCGGTAACTCGCAGGCCACCGGAACCCGCGCG
GTCACCAGCGGCACCCCCGACCCCGCGTGGCTGCGGCGCTGCAAGGAGGTCAACGACCACGCCGTCGCCGCGGTGCGCGA
ACAACTCGCCGCCCACCCGCTGACCACCGGACTGCACGTCGCCGCAGCGGTGGCCGATGCGGTCCGGCCGGGGGATCAGC
TGGTGCTGGGCGCGAGCAACCCGGTGCGCGACGCCGCGCTGGTCGGGTTCACGCCCCACGGTGTGCAGGTGCGCTCCAAC
CGCGGGGTGGCCGGTATCGACGGCACGGTGTCGACGGCGATCGGCGCCGCACTGGCCCACGACCGCACCGGCGGCCGCAC
CATCGCGTTGATGGGCGACCTGACGTTCGTACACGACAGCTCGGGCCTGTTGATCGGCCCGACCGAACCGACCCCGCGCA
ACCTGACGATCGTGGTGTCCAACGACAACGGCGGCGGCATCTTCGAACTGCTCGAACAGGGCGATCCCCGCTTCAGCGAC
GTTTCGTCTCGGGTGTTCGGCACCCCGCACGACGTCGACGTCGGCGCGCTGTGCCGCGCCTATCACGTCGACAACCGCCA
GATCGAGGTCGGCCAGCTCGCCGACGCACTCGACGAACCGCACGAGGGGATGCGCGTGCTGGAGGTGAAAGCCGACAGGT
CGTCGCTGCGCGCGCTGCATGCCTCGATCAAGGCCGCGCTGTGA

Upstream 100 bases:

>100_bases
GCTATTTGGAGATCCCCGACGCCGGACACCTCGGCTTCATCGAGCGGCCGCAGGAAGTCAACGCCGCGGCGCTGAAATTC
TTCGCCGATATCCTGTAGCG

Downstream 100 bases:

>100_bases
GGCTGGCCGAACGGGTGGCCGCCGTCCGCCGCGTGGTCATCCCGCATCTGTGGGGCGACGGGGACGAGACCCGCGCCGAG
CGGATCATCCGGCGGGTGCG

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MNPSTAQARVVVDELVRGGVHDVVLCPGSRNAPLAFALADADRAGRLRLHVRIDERTAGFLAIGLAVADRAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTFEQLGYFGNQVRANISLGLAPELSSGSPGDMTSLNAQWRSA
TCRVVVAATGSRSANAGPVQFDIPLREPLVPTFDDDGSCPPGRPDGKPWTHTPPVTFDQPLDIDLTPDTVVIAGHGAGVH
PNLADLPTVAEPTAPPAANPLHPMALRLLRPKQVIMLGRPTLHRPVSALLADPSVPVYALTTGPRWPDVSGNSQATGTRA
VTSGTPDPAWLRRCKEVNDHAVAAVREQLAAHPLTTGLHVAAAVADAVRPGDQLVLGASNPVRDAALVGFTPHGVQVRSN
RGVAGIDGTVSTAIGAALAHDRTGGRTIALMGDLTFVHDSSGLLIGPTEPTPRNLTIVVSNDNGGGIFELLEQGDPRFSD
VSSRVFGTPHDVDVGALCRAYHVDNRQIEVGQLADALDEPHEGMRVLEVKADRSSLRALHASIKAAL

Sequences:

>Translated_547_residues
MNPSTAQARVVVDELVRGGVHDVVLCPGSRNAPLAFALADADRAGRLRLHVRIDERTAGFLAIGLAVADRAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTFEQLGYFGNQVRANISLGLAPELSSGSPGDMTSLNAQWRSA
TCRVVVAATGSRSANAGPVQFDIPLREPLVPTFDDDGSCPPGRPDGKPWTHTPPVTFDQPLDIDLTPDTVVIAGHGAGVH
PNLADLPTVAEPTAPPAANPLHPMALRLLRPKQVIMLGRPTLHRPVSALLADPSVPVYALTTGPRWPDVSGNSQATGTRA
VTSGTPDPAWLRRCKEVNDHAVAAVREQLAAHPLTTGLHVAAAVADAVRPGDQLVLGASNPVRDAALVGFTPHGVQVRSN
RGVAGIDGTVSTAIGAALAHDRTGGRTIALMGDLTFVHDSSGLLIGPTEPTPRNLTIVVSNDNGGGIFELLEQGDPRFSD
VSSRVFGTPHDVDVGALCRAYHVDNRQIEVGQLADALDEPHEGMRVLEVKADRSSLRALHASIKAAL
>Mature_547_residues
MNPSTAQARVVVDELVRGGVHDVVLCPGSRNAPLAFALADADRAGRLRLHVRIDERTAGFLAIGLAVADRAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTFEQLGYFGNQVRANISLGLAPELSSGSPGDMTSLNAQWRSA
TCRVVVAATGSRSANAGPVQFDIPLREPLVPTFDDDGSCPPGRPDGKPWTHTPPVTFDQPLDIDLTPDTVVIAGHGAGVH
PNLADLPTVAEPTAPPAANPLHPMALRLLRPKQVIMLGRPTLHRPVSALLADPSVPVYALTTGPRWPDVSGNSQATGTRA
VTSGTPDPAWLRRCKEVNDHAVAAVREQLAAHPLTTGLHVAAAVADAVRPGDQLVLGASNPVRDAALVGFTPHGVQVRSN
RGVAGIDGTVSTAIGAALAHDRTGGRTIALMGDLTFVHDSSGLLIGPTEPTPRNLTIVVSNDNGGGIFELLEQGDPRFSD
VSSRVFGTPHDVDVGALCRAYHVDNRQIEVGQLADALDEPHEGMRVLEVKADRSSLRALHASIKAAL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=512, Percent_Identity=30.6640625, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001 [H]

Pfam domain/function: PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 57160; Mature: 57160

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSTAQARVVVDELVRGGVHDVVLCPGSRNAPLAFALADADRAGRLRLHVRIDERTAGF
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCE
LAIGLAVADRAPVCVAMTSGTAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTF
EEEEEECCCCCCEEEEEECCCEEECCCCHHEECCCCEEEEEEEECCCCEEEEECCCCHHH
EQLGYFGNQVRANISLGLAPELSSGSPGDMTSLNAQWRSATCRVVVAATGSRSANAGPVQ
HHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEE
FDIPLREPLVPTFDDDGSCPPGRPDGKPWTHTPPVTFDQPLDIDLTPDTVVIAGHGAGVH
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCCC
PNLADLPTVAEPTAPPAANPLHPMALRLLRPKQVIMLGRPTLHRPVSALLADPSVPVYAL
CCHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEE
TTGPRWPDVSGNSQATGTRAVTSGTPDPAWLRRCKEVNDHAVAAVREQLAAHPLTTGLHV
ECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
AAAVADAVRPGDQLVLGASNPVRDAALVGFTPHGVQVRSNRGVAGIDGTVSTAIGAALAH
HHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCCEEEECCCCEECCCCHHHHHHHHHHHC
DRTGGRTIALMGDLTFVHDSSGLLIGPTEPTPRNLTIVVSNDNGGGIFELLEQGDPRFSD
CCCCCEEEEEEEEEEEEECCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHCCCCCHHH
VSSRVFGTPHDVDVGALCRAYHVDNRQIEVGQLADALDEPHEGMRVLEVKADRSSLRALH
HHHHHCCCCCCCCHHHHHEEEECCCCEEEHHHHHHHHCCCCCCCEEEEEECCHHHHHHHH
ASIKAAL
HHHHHCC
>Mature Secondary Structure
MNPSTAQARVVVDELVRGGVHDVVLCPGSRNAPLAFALADADRAGRLRLHVRIDERTAGF
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCE
LAIGLAVADRAPVCVAMTSGTAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTF
EEEEEECCCCCCEEEEEECCCEEECCCCHHEECCCCEEEEEEEECCCCEEEEECCCCHHH
EQLGYFGNQVRANISLGLAPELSSGSPGDMTSLNAQWRSATCRVVVAATGSRSANAGPVQ
HHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEE
FDIPLREPLVPTFDDDGSCPPGRPDGKPWTHTPPVTFDQPLDIDLTPDTVVIAGHGAGVH
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCCC
PNLADLPTVAEPTAPPAANPLHPMALRLLRPKQVIMLGRPTLHRPVSALLADPSVPVYAL
CCHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEE
TTGPRWPDVSGNSQATGTRAVTSGTPDPAWLRRCKEVNDHAVAAVREQLAAHPLTTGLHV
ECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
AAAVADAVRPGDQLVLGASNPVRDAALVGFTPHGVQVRSNRGVAGIDGTVSTAIGAALAH
HHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCCEEEECCCCEECCCCHHHHHHHHHHHC
DRTGGRTIALMGDLTFVHDSSGLLIGPTEPTPRNLTIVVSNDNGGGIFELLEQGDPRFSD
CCCCCEEEEEEEEEEEEECCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHCCCCCHHH
VSSRVFGTPHDVDVGALCRAYHVDNRQIEVGQLADALDEPHEGMRVLEVKADRSSLRALH
HHHHHCCCCCCCCHHHHHEEEECCCCEEEHHHHHHHHCCCCCCCEEEEEECCHHHHHHHH
ASIKAAL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA