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The map label for this gene is purA [H]

Identifier: 108797495

GI number: 108797495

Start: 571296

End: 572591

Strand: Direct

Name: purA [H]

Synonym: Mmcs_0515

Alternate gene names: 108797495

Gene position: 571296-572591 (Clockwise)

Preceding gene: 108797493

Following gene: 108797496

Centisome position: 10.01

GC content: 67.67

Gene sequence:

>1296_bases
ATGCCGGCAATCGTGCTCATCGGCGCCCAGTGGGGCGACGAGGGCAAAGGAAAGGCCACCGATCTACTCGGTGGCCGCGT
GCAGTGGGTCGTGCGGTACCAGGGCGGCAACAACGCCGGTCACACCGTCGTGCTCCCCACCGGCGAGAACTTCGCGCTGC
ACCTCATCCCGTCGGGGATCCTCACCCCCGGCGTCACCAACGTCATCGGCAACGGCGTCGTCGTCGATCCGGGGGTGCTG
CTCACCGAGCTGCGGGGTCTGGAGGAGCGCGGCGTGGACACCGAACGCCTGCTGATCTCCGCCGACGCCCACCTGCTGAT
GCCGTATCACGTCGCGATCGACAAGGTGGTCGAACGATATGCGGGCAGCAAGAAGATCGGCACCACCGGCCGCGGCATCG
GACCGTGCTACCAGGACAAGATCGCCCGCCAGGGCATCCGTGTGGCCGACGTGCTCGACCCGGCCGTGCTGGCCGAGAAG
ATCGAGGGCGCACTGGAGCTGAAGAACCAGGTGCTCGTCAAGATCTACAACCGCAAGGCGCTGGAGCCCGCCGAGGTGGT
CGAGAACCTGTTGGAGCAGGCCGAGGGGTTCAAGCACCGCATCGCCGACGCACGGCTGCTGCTCAACCAGGCGCTCGAGC
GCGACGAGATCGTGCTGCTGGAGGGGTCGCAGGGCACGCTGCTCGACGTCGACCACGGCACGTACCCGTACGTCACGTCG
TCGAATCCGACGGCCGGCGGCGCATCGGTGGGTTCGGGTATCGGACCCACCCGCATCACCACCGTGCTCGGGATTCTGAA
GGCGTACACGACGCGGGTCGGCTCGGGTCCGTTCCCGACCGAATTGTTCGACGAGCACGGCGCCTATCTCGCCAAGACCG
GCGGCGAGGTCGGTGTCACCACCGGCCGCGCCCGCCGCTGCGGCTGGTTCGACGCGGTGATCGCGCGGTACGCGACCCGA
GTCAACGGCATCACCGACTACTTCCTCACCAAACTCGACGTGCTGTCCAGCCTGGAGACGGTGCCGATCTGCGTCGGCTA
CACCATCGACGGCAAGCGCACCGACGAGATGCCGATGACGCAGAGCGACATCGCGCGCGCCGAACCGGTCTACGAGGAAC
TGCCCGGCTGGTGGGAGGACATCTCCGGCGCCCGGGAGTTCGACGACCTGCCCGCGAAGGCGCGCGACTATGTGCTGCGG
CTCGAAGAGCTTGCCGGAGCGCATGTTTCGTGTATCGGCGTGGGCCCGGGACGGGATCAGACCATTGTGCGGCGTGACGT
GCTGGCGCCGTCGTGA

Upstream 100 bases:

>100_bases
CCGCCTGCGGCCCTGTCTCTGGCACTCAAGAGCGATCCTCCCGACCTGCTGGGATAGGGTGTGTCACCGGCTGCACCACA
TCAAAGCGGGAGACTAAGCC

Downstream 100 bases:

>100_bases
GCTCCGACTACGGTCTGGATCCGCGCCGCGTCGACCCGGAATACGACCGCCACGGCGGGTTCCCGGTGTTCGAGGCCGCC
GAACCCGGACCTGGTTTCGA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGILTPGVTNVIGNGVVVDPGVL
LTELRGLEERGVDTERLLISADAHLLMPYHVAIDKVVERYAGSKKIGTTGRGIGPCYQDKIARQGIRVADVLDPAVLAEK
IEGALELKNQVLVKIYNRKALEPAEVVENLLEQAEGFKHRIADARLLLNQALERDEIVLLEGSQGTLLDVDHGTYPYVTS
SNPTAGGASVGSGIGPTRITTVLGILKAYTTRVGSGPFPTELFDEHGAYLAKTGGEVGVTTGRARRCGWFDAVIARYATR
VNGITDYFLTKLDVLSSLETVPICVGYTIDGKRTDEMPMTQSDIARAEPVYEELPGWWEDISGAREFDDLPAKARDYVLR
LEELAGAHVSCIGVGPGRDQTIVRRDVLAPS

Sequences:

>Translated_431_residues
MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGILTPGVTNVIGNGVVVDPGVL
LTELRGLEERGVDTERLLISADAHLLMPYHVAIDKVVERYAGSKKIGTTGRGIGPCYQDKIARQGIRVADVLDPAVLAEK
IEGALELKNQVLVKIYNRKALEPAEVVENLLEQAEGFKHRIADARLLLNQALERDEIVLLEGSQGTLLDVDHGTYPYVTS
SNPTAGGASVGSGIGPTRITTVLGILKAYTTRVGSGPFPTELFDEHGAYLAKTGGEVGVTTGRARRCGWFDAVIARYATR
VNGITDYFLTKLDVLSSLETVPICVGYTIDGKRTDEMPMTQSDIARAEPVYEELPGWWEDISGAREFDDLPAKARDYVLR
LEELAGAHVSCIGVGPGRDQTIVRRDVLAPS
>Mature_430_residues
PAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGILTPGVTNVIGNGVVVDPGVLL
TELRGLEERGVDTERLLISADAHLLMPYHVAIDKVVERYAGSKKIGTTGRGIGPCYQDKIARQGIRVADVLDPAVLAEKI
EGALELKNQVLVKIYNRKALEPAEVVENLLEQAEGFKHRIADARLLLNQALERDEIVLLEGSQGTLLDVDHGTYPYVTSS
NPTAGGASVGSGIGPTRITTVLGILKAYTTRVGSGPFPTELFDEHGAYLAKTGGEVGVTTGRARRCGWFDAVIARYATRV
NGITDYFLTKLDVLSSLETVPICVGYTIDGKRTDEMPMTQSDIARAEPVYEELPGWWEDISGAREFDDLPAKARDYVLRL
EELAGAHVSCIGVGPGRDQTIVRRDVLAPS

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=430, Percent_Identity=44.4186046511628, Blast_Score=324, Evalue=8e-89,
Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=43.0555555555556, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI40316944, Length=401, Percent_Identity=43.3915211970075, Blast_Score=291, Evalue=9e-79,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=51.0588235294118, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI25146553, Length=435, Percent_Identity=43.9080459770115, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI25146555, Length=435, Percent_Identity=43.9080459770115, Blast_Score=337, Evalue=9e-93,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=41.3953488372093, Blast_Score=334, Evalue=1e-92,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=41.7061611374408, Blast_Score=315, Evalue=4e-86,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46369; Mature: 46238

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGI
CCEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCC
LTPGVTNVIGNGVVVDPGVLLTELRGLEERGVDTERLLISADAHLLMPYHVAIDKVVERY
CCCCHHHHHCCCEEECCCHHHHHHHCHHHHCCCHHHEEEECCCEEEEHHHHHHHHHHHHH
AGSKKIGTTGRGIGPCYQDKIARQGIRVADVLDPAVLAEKIEGALELKNQVLVKIYNRKA
CCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
LEPAEVVENLLEQAEGFKHRIADARLLLNQALERDEIVLLEGSQGTLLDVDHGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCCEEEC
SNPTAGGASVGSGIGPTRITTVLGILKAYTTRVGSGPFPTELFDEHGAYLAKTGGEVGVT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCCEECC
TGRARRCGWFDAVIARYATRVNGITDYFLTKLDVLSSLETVPICVGYTIDGKRTDEMPMT
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCC
QSDIARAEPVYEELPGWWEDISGAREFDDLPAKARDYVLRLEELAGAHVSCIGVGPGRDQ
HHHHHHCCCHHHHCCCHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCC
TIVRRDVLAPS
HHHHHHCCCCC
>Mature Secondary Structure 
PAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGI
CEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCC
LTPGVTNVIGNGVVVDPGVLLTELRGLEERGVDTERLLISADAHLLMPYHVAIDKVVERY
CCCCHHHHHCCCEEECCCHHHHHHHCHHHHCCCHHHEEEECCCEEEEHHHHHHHHHHHHH
AGSKKIGTTGRGIGPCYQDKIARQGIRVADVLDPAVLAEKIEGALELKNQVLVKIYNRKA
CCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
LEPAEVVENLLEQAEGFKHRIADARLLLNQALERDEIVLLEGSQGTLLDVDHGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCCEEEC
SNPTAGGASVGSGIGPTRITTVLGILKAYTTRVGSGPFPTELFDEHGAYLAKTGGEVGVT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCCEECC
TGRARRCGWFDAVIARYATRVNGITDYFLTKLDVLSSLETVPICVGYTIDGKRTDEMPMT
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCC
QSDIARAEPVYEELPGWWEDISGAREFDDLPAKARDYVLRLEELAGAHVSCIGVGPGRDQ
HHHHHHCCCHHHHCCCHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCC
TIVRRDVLAPS
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA