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The map label for this gene is greA [H]

Identifier: 108763574

GI number: 108763574

Start: 3957094

End: 3957612

Strand: Direct

Name: greA [H]

Synonym: MXAN_3405

Alternate gene names: 108763574

Gene position: 3957094-3957612 (Clockwise)

Preceding gene: 108764014

Following gene: 108763623

Centisome position: 43.3

GC content: 65.32

Gene sequence:

>519_bases
ATGAGCAGCGGGAGCGACAACATCCCGATGACCCCGTCCGGACTGCGCAAGCTGAAGGAGGAACTGAAGCACCTCCAGTC
CGTCGAGCGGGGGAAGATCTCGCGCGAGATCGAGGTCGCTCGCGCACATGGTGACCTGCGTGAGAACGCGGAGTACCACG
CGGCGAAGGAGAAACAGTCGCACATCGAGGGGCGCATCCTGACCCTCAACGACTGGATTGCCCGCGCGGAGGTCATCGAC
CCAAAGAAGCTCGGCGGCGACAAGGTCATTTTCGGGGCGACGGTGGACCTGCTCGATACCGAAACCGACAAGCCGGTGAG
CTACCGGCTCGTGGGGGAGATCGAGGCAGACCTGAAGAAGCGGTGGATTGCCGTCACCTCGCCGGTGGCGCGCGCGCTCA
TCGGTAAAAAGGTGGGCGACATCGCCACGGTGCAGAGCCCCGGCGGCGTGCGCGAGCTGGAAGTCCAGGAGATCCGCTTC
GAGGAGCCCGAGGAAGAGGCCCCGGCGAGCGGGAGCTGA

Upstream 100 bases:

>100_bases
TACATTGGGCTTTTGTTTGGTCGCGGGGGCCGGGCTCCGGAAGAGGAGCGCCGGCCCTCCATCTTATTTGAACCTGGGAG
TGGTGGAAGGAGCTGTGTCG

Downstream 100 bases:

>100_bases
GCCTCGGAGGTCGCAGTGAGTTGCCGGGGGAGCGGGCCAGGGCTCGCTTCCCCGGTCTCGTTTTGTGGGAGACGTCATCC
CCGTTCCGTAGGATGTACGG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MSSGSDNIPMTPSGLRKLKEELKHLQSVERGKISREIEVARAHGDLRENAEYHAAKEKQSHIEGRILTLNDWIARAEVID
PKKLGGDKVIFGATVDLLDTETDKPVSYRLVGEIEADLKKRWIAVTSPVARALIGKKVGDIATVQSPGGVRELEVQEIRF
EEPEEEAPASGS

Sequences:

>Translated_172_residues
MSSGSDNIPMTPSGLRKLKEELKHLQSVERGKISREIEVARAHGDLRENAEYHAAKEKQSHIEGRILTLNDWIARAEVID
PKKLGGDKVIFGATVDLLDTETDKPVSYRLVGEIEADLKKRWIAVTSPVARALIGKKVGDIATVQSPGGVRELEVQEIRF
EEPEEEAPASGS
>Mature_171_residues
SSGSDNIPMTPSGLRKLKEELKHLQSVERGKISREIEVARAHGDLRENAEYHAAKEKQSHIEGRILTLNDWIARAEVIDP
KKLGGDKVIFGATVDLLDTETDKPVSYRLVGEIEADLKKRWIAVTSPVARALIGKKVGDIATVQSPGGVRELEVQEIRFE
EPEEEAPASGS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=48.7012987012987, Blast_Score=145, Evalue=1e-36,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=34.6153846153846, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18947; Mature: 18815

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGSDNIPMTPSGLRKLKEELKHLQSVERGKISREIEVARAHGDLRENAEYHAAKEKQS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCHHHHHHHHHH
HIEGRILTLNDWIARAEVIDPKKLGGDKVIFGATVDLLDTETDKPVSYRLVGEIEADLKK
HHCCEEEEEHHHHHHHHCCCHHHCCCCEEEEEEEEEEECCCCCCCEEEEEEHHHHHHHHH
RWIAVTSPVARALIGKKVGDIATVQSPGGVRELEVQEIRFEEPEEEAPASGS
HEEHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEHHECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSGSDNIPMTPSGLRKLKEELKHLQSVERGKISREIEVARAHGDLRENAEYHAAKEKQS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCHHHHHHHHHH
HIEGRILTLNDWIARAEVIDPKKLGGDKVIFGATVDLLDTETDKPVSYRLVGEIEADLKK
HHCCEEEEEHHHHHHHHCCCHHHCCCCEEEEEEEEEEECCCCCCCEEEEEEHHHHHHHHH
RWIAVTSPVARALIGKKVGDIATVQSPGGVRELEVQEIRFEEPEEEAPASGS
HEEHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEHHECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA