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The map label for this gene is actB [H]

Identifier: 108762849

GI number: 108762849

Start: 3763806

End: 3765467

Strand: Direct

Name: actB [H]

Synonym: MXAN_3214

Alternate gene names: 108762849

Gene position: 3763806-3765467 (Clockwise)

Preceding gene: 108763553

Following gene: 108760626

Centisome position: 41.18

GC content: 70.7

Gene sequence:

>1662_bases
ATGGACCGGATCGCGGTGCTGGTGGTGGATGACGAAGAGGCGGTGCGCACGTTTCTGTCCGAGCTGCTCGGAAGTGCGGG
GTACCAGGTGCGCTGTGCCGCGAGTGGCACCCAGGCGCTGGAGATGCTCTCAGGGGGCTCCTTCGACGCGGTGTTGCTGG
ACGTGGTGATGCCGGAGATGAGCGGCCTGGAAGTGCTGCGCCGTTACCAGTCCATGGGCGGCGGCGCGCCCGTCATCGTG
CTCAGCGCGCTGGCGGGGGCGGACGACGCCGTTCGGGCGATGAAGATGGGCGCCAGCGACTACCTGGCCAAGCCGTTCGG
CAACGACGAGCTGCAGGACGCGCTGGCCCGGGCCCTGGGGACGCGGGCGCCGGAGCGTCAGGCCGTGGCGCCCATCCTGC
CCTCGCGCACGGTGCTGCCGGTGGTGGATTCGGCGCAGGAGCAGCGCGTCCTCATCTCCACCTCGCCGGCCATGCGCCGC
GCGCGTGCGCTGGTGGAGCGCATCGCCGACACGGACGTCCCCGTGCTGCTGCTGGGTGAGTCCGGTACGGGCAAGGAGGT
CATCGCGCGGGAGATTCACGCGCGCAGCCAGCGGCGCAACCGTCCGTTCATCAAGGTGAACTGCGCGGCGCTGCCCGGCG
AGCTGCTGGAGAGTGAGCTGTTCGGCCACGAGCGCGGCGCCTTCACCGGCGCCACTGCCGAGAAGCCCGGCAAGTTCGAA
CTGGCGGACCAGGGCACCATCTTCCTGGACGAGATTGGCGAAATGGCCATCCGGCTCCAGGCCAAGCTGCTCCAGGTGCT
CCAGGACGAGGAGTTCTTCCGCGTCGGTGGCAAGAAGAGCGTGCGCGTGGACAGCCGCGTCGTCGTGGCCACCAACCGCG
ACCTGGAGAAGGAAATCGCGCTGGGCAACTTCCGCGAGGACCTCTTCTATCGCCTCAACGTGGTGGCCATCCGCCTGCCG
GCGCTGCGCGAGCGCCGCGAGGACGTGGTTCCGCTGACGGACCACTTCCTCAAGAAGTACGGCCGAGGCTACCTCAGCGG
CGTGTCCGAGCTGCCCACGGAGGTGCTGCACGCCTTCGCGGACTACGACTGGCCGGGCAACGTGCGCGAGCTGGAGAACA
TGGTCCGCCGGCTGTGCGTGCTGAAGGACCCGACGCTGGTGCTCGACGAGCTGCGGGCCGCGGTCCGCGCTCCCGCGAGC
GCGCCGTCCCTGCCCACGTCCTACGCCGGTGACGACGGTGGCCCCTTCGGCCGCGCGCACGACGAGGGCTTCCGCACGCC
GCCGCCCGCCGTGCAGGTGCTGGAGATGCCGTCGCGCGGCGCCGCCTTTGGGCTGGCGTCCGTCGCGCCGCTGGCGCCGC
CCGCGCACGCGCCCATGGTGATGGAGTCCGCGAACGCGGTGGTGTCCGCGCCGCGCTACGTCAATCCCTTCGACGTGCCG
CAGCCGCCGCCGCCGCCCGCGCCCACCGGGGAGCTGTCGCTGAAGGACATCGGCAAACGGGCGGCGATGCTGGCCGAGCG
CGAGGCGATTCTGGCCATGCTCCAACGCACCGCGTGGAACAAGCGGCGCGCGGCCACCAAGCTGCGCATCAGCTACAAGG
CGCTCCTCTACAAAATCAAGGAGTGCGGCATCATCGACCCGCGCGCGAGCGCGGAGCTGTAG

Upstream 100 bases:

>100_bases
GACGCGCGCGTGAGTTTCGCCGGATGGTCGGGAGCACGTCCCGGTTGTCGGTGTGAGGGTGGGTCGGACTTCGATGCAGC
GGGCGAGTGGAGGCGAGGGG

Downstream 100 bases:

>100_bases
GTGAGCCGTAACCGTCGCTTGCACTGTCCCCGCGGTCTCGCTATCGCGGGGACATGACGCCTCCTCTCGTCCTCCTGGGG
TCTGGGTACACGCTCACGCG

Product: sigma-54 activator protein ActB

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MDRIAVLVVDDEEAVRTFLSELLGSAGYQVRCAASGTQALEMLSGGSFDAVLLDVVMPEMSGLEVLRRYQSMGGGAPVIV
LSALAGADDAVRAMKMGASDYLAKPFGNDELQDALARALGTRAPERQAVAPILPSRTVLPVVDSAQEQRVLISTSPAMRR
ARALVERIADTDVPVLLLGESGTGKEVIAREIHARSQRRNRPFIKVNCAALPGELLESELFGHERGAFTGATAEKPGKFE
LADQGTIFLDEIGEMAIRLQAKLLQVLQDEEFFRVGGKKSVRVDSRVVVATNRDLEKEIALGNFREDLFYRLNVVAIRLP
ALRERREDVVPLTDHFLKKYGRGYLSGVSELPTEVLHAFADYDWPGNVRELENMVRRLCVLKDPTLVLDELRAAVRAPAS
APSLPTSYAGDDGGPFGRAHDEGFRTPPPAVQVLEMPSRGAAFGLASVAPLAPPAHAPMVMESANAVVSAPRYVNPFDVP
QPPPPPAPTGELSLKDIGKRAAMLAEREAILAMLQRTAWNKRRAATKLRISYKALLYKIKECGIIDPRASAEL

Sequences:

>Translated_553_residues
MDRIAVLVVDDEEAVRTFLSELLGSAGYQVRCAASGTQALEMLSGGSFDAVLLDVVMPEMSGLEVLRRYQSMGGGAPVIV
LSALAGADDAVRAMKMGASDYLAKPFGNDELQDALARALGTRAPERQAVAPILPSRTVLPVVDSAQEQRVLISTSPAMRR
ARALVERIADTDVPVLLLGESGTGKEVIAREIHARSQRRNRPFIKVNCAALPGELLESELFGHERGAFTGATAEKPGKFE
LADQGTIFLDEIGEMAIRLQAKLLQVLQDEEFFRVGGKKSVRVDSRVVVATNRDLEKEIALGNFREDLFYRLNVVAIRLP
ALRERREDVVPLTDHFLKKYGRGYLSGVSELPTEVLHAFADYDWPGNVRELENMVRRLCVLKDPTLVLDELRAAVRAPAS
APSLPTSYAGDDGGPFGRAHDEGFRTPPPAVQVLEMPSRGAAFGLASVAPLAPPAHAPMVMESANAVVSAPRYVNPFDVP
QPPPPPAPTGELSLKDIGKRAAMLAEREAILAMLQRTAWNKRRAATKLRISYKALLYKIKECGIIDPRASAEL
>Mature_553_residues
MDRIAVLVVDDEEAVRTFLSELLGSAGYQVRCAASGTQALEMLSGGSFDAVLLDVVMPEMSGLEVLRRYQSMGGGAPVIV
LSALAGADDAVRAMKMGASDYLAKPFGNDELQDALARALGTRAPERQAVAPILPSRTVLPVVDSAQEQRVLISTSPAMRR
ARALVERIADTDVPVLLLGESGTGKEVIAREIHARSQRRNRPFIKVNCAALPGELLESELFGHERGAFTGATAEKPGKFE
LADQGTIFLDEIGEMAIRLQAKLLQVLQDEEFFRVGGKKSVRVDSRVVVATNRDLEKEIALGNFREDLFYRLNVVAIRLP
ALRERREDVVPLTDHFLKKYGRGYLSGVSELPTEVLHAFADYDWPGNVRELENMVRRLCVLKDPTLVLDELRAAVRAPAS
APSLPTSYAGDDGGPFGRAHDEGFRTPPPAVQVLEMPSRGAAFGLASVAPLAPPAHAPMVMESANAVVSAPRYVNPFDVP
QPPPPPAPTGELSLKDIGKRAAMLAEREAILAMLQRTAWNKRRAATKLRISYKALLYKIKECGIIDPRASAEL

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=408, Percent_Identity=42.8921568627451, Blast_Score=336, Evalue=3e-93,
Organism=Escherichia coli, GI1790437, Length=380, Percent_Identity=43.9473684210526, Blast_Score=314, Evalue=8e-87,
Organism=Escherichia coli, GI1790299, Length=416, Percent_Identity=41.3461538461538, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788905, Length=386, Percent_Identity=39.6373056994819, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1789087, Length=231, Percent_Identity=52.8138528138528, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=51.9480519480519, Blast_Score=248, Evalue=9e-67,
Organism=Escherichia coli, GI1789233, Length=247, Percent_Identity=47.3684210526316, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI87082152, Length=248, Percent_Identity=48.3870967741936, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786524, Length=248, Percent_Identity=44.3548387096774, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI87081872, Length=217, Percent_Identity=47.0046082949309, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787583, Length=227, Percent_Identity=40.5286343612335, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789828, Length=242, Percent_Identity=36.3636363636364, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI87081858, Length=228, Percent_Identity=30.7017543859649, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1786784, Length=133, Percent_Identity=32.3308270676692, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI145693157, Length=115, Percent_Identity=38.2608695652174, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1789809, Length=106, Percent_Identity=35.8490566037736, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787487, Length=100, Percent_Identity=37, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1786911, Length=124, Percent_Identity=38.7096774193548, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082012, Length=124, Percent_Identity=32.258064516129, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789402, Length=101, Percent_Identity=32.6732673267327, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787375, Length=106, Percent_Identity=33.9622641509434, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786599, Length=108, Percent_Identity=34.2592592592593, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790860, Length=133, Percent_Identity=30.0751879699248, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=116, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=141, Percent_Identity=31.2056737588652, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 60052; Mature: 60052

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIAVLVVDDEEAVRTFLSELLGSAGYQVRCAASGTQALEMLSGGSFDAVLLDVVMPEM
CCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHCCC
SGLEVLRRYQSMGGGAPVIVLSALAGADDAVRAMKMGASDYLAKPFGNDELQDALARALG
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHC
TRAPERQAVAPILPSRTVLPVVDSAQEQRVLISTSPAMRRARALVERIADTDVPVLLLGE
CCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEECC
SGTGKEVIAREIHARSQRRNRPFIKVNCAALPGELLESELFGHERGAFTGATAEKPGKFE
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEE
LADQGTIFLDEIGEMAIRLQAKLLQVLQDEEFFRVGGKKSVRVDSRVVVATNRDLEKEIA
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCEEEEEECCCHHHHHH
LGNFREDLFYRLNVVAIRLPALRERREDVVPLTDHFLKKYGRGYLSGVSELPTEVLHAFA
HCCHHHHHHHHHHHHEEECHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
DYDWPGNVRELENMVRRLCVLKDPTLVLDELRAAVRAPASAPSLPTSYAGDDGGPFGRAH
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DEGFRTPPPAVQVLEMPSRGAAFGLASVAPLAPPAHAPMVMESANAVVSAPRYVNPFDVP
CCCCCCCCCHHHHEECCCCCCHHCHHHCCCCCCCCCCCHHHHCCCHHCCCCCCCCCCCCC
QPPPPPAPTGELSLKDIGKRAAMLAEREAILAMLQRTAWNKRRAATKLRISYKALLYKIK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ECGIIDPRASAEL
HCCCCCCCCCCCC
>Mature Secondary Structure
MDRIAVLVVDDEEAVRTFLSELLGSAGYQVRCAASGTQALEMLSGGSFDAVLLDVVMPEM
CCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHCCC
SGLEVLRRYQSMGGGAPVIVLSALAGADDAVRAMKMGASDYLAKPFGNDELQDALARALG
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHC
TRAPERQAVAPILPSRTVLPVVDSAQEQRVLISTSPAMRRARALVERIADTDVPVLLLGE
CCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEECC
SGTGKEVIAREIHARSQRRNRPFIKVNCAALPGELLESELFGHERGAFTGATAEKPGKFE
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEE
LADQGTIFLDEIGEMAIRLQAKLLQVLQDEEFFRVGGKKSVRVDSRVVVATNRDLEKEIA
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCEEEEEECCCHHHHHH
LGNFREDLFYRLNVVAIRLPALRERREDVVPLTDHFLKKYGRGYLSGVSELPTEVLHAFA
HCCHHHHHHHHHHHHEEECHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
DYDWPGNVRELENMVRRLCVLKDPTLVLDELRAAVRAPASAPSLPTSYAGDDGGPFGRAH
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DEGFRTPPPAVQVLEMPSRGAAFGLASVAPLAPPAHAPMVMESANAVVSAPRYVNPFDVP
CCCCCCCCCHHHHEECCCCCCHHCHHHCCCCCCCCCCCHHHHCCCHHCCCCCCCCCCCCC
QPPPPPAPTGELSLKDIGKRAAMLAEREAILAMLQRTAWNKRRAATKLRISYKALLYKIK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ECGIIDPRASAEL
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]