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The map label for this gene is hisF

Identifier: 108762116

GI number: 108762116

Start: 5184038

End: 5184799

Strand: Reverse

Name: hisF

Synonym: MXAN_4224

Alternate gene names: 108762116

Gene position: 5184799-5184038 (Counterclockwise)

Preceding gene: 108758246

Following gene: 108759195

Centisome position: 56.73

GC content: 70.73

Gene sequence:

>762_bases
ATGCTCACGCGGCGGCTCGTCGTCTGTCTGGACGTGAAGGGCGGACGCGTGGTGAAGGGCGTCCAGTTCGAGGGACTTCG
CGACGTGGGTGACCCGGTGGAATTGGCCCGGCGCTACGAAGCGGAGGGGGCGGATGAGCTGACGTTCCTCGACATCTCCG
CCAGCGCCGAGGAGCGGGACACCTTGTGGGAGCTGGTGCGGCGCACCGCCGAGCAGCTCTTCATTCCCCTGGCTGTCGGC
GGCGGGGTGCGCACGGTCGATGACGTGGGCCGGGCGCTGCGTGCGGGCGCGGACAAGGTGAGCATCAACTCCGCGGCGGT
GGCCAACCCGGCGCTGCTCACCGCGTGCGCGGAGCGCTTCGGTGCGCAGTGCGTGGTGGCCAGCATCGACGCGAAGAGAG
ACGGCGACCGCTGGCGCGTCTACACCCATGGCGGCAGGAAGCCCACGGACCTGGACGCGGTGGCCTGGGCGCGTGAGTGT
GTGGCTCGCGGGGCAGGGGAAGTGCTCCTCACCAGCATCGACCGGGATGGCGCGCGGACGGGCTATGACCTGGCGTTGAC
GCGGGCCGTGTCGGAAGCGGTCGACGTGCCCGTCATCGCCTCGGGCGGCGCGGGCAGCGCGGCGCACGTCCGGGCTGCAT
TCCAGGAGGGAGGCGCTGATGCGGCATTGGTCGCGGGCATCCTTCACGACGGCGTCACTACCGTGGGCGCCATCAAGGCG
CTGCTCCGCGAGAGCGGCCTTCACATTCGGAGCCTGACATGA

Upstream 100 bases:

>100_bases
AGGCGTCCTTCAAGGCGCTGGGGCTGGCGCTGCGGGATGCCATGGTGGACTCCGGCTCGGTGTTCAGCATGAAGGGCTCC
GTGTCCCTGGAGGTGAAGTG

Downstream 100 bases:

>100_bases
CGGACTCGGGTAATCTGATGCAGGCCGCCGCCGAGGTGGCGCGGATAGCGGGTGACGCGGCGTTGGGGTTCTTCCGCGGT
GGCATCGCGGTGGACACGAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLTRRLVVCLDVKGGRVVKGVQFEGLRDVGDPVELARRYEAEGADELTFLDISASAEERDTLWELVRRTAEQLFIPLAVG
GGVRTVDDVGRALRAGADKVSINSAAVANPALLTACAERFGAQCVVASIDAKRDGDRWRVYTHGGRKPTDLDAVAWAREC
VARGAGEVLLTSIDRDGARTGYDLALTRAVSEAVDVPVIASGGAGSAAHVRAAFQEGGADAALVAGILHDGVTTVGAIKA
LLRESGLHIRSLT

Sequences:

>Translated_253_residues
MLTRRLVVCLDVKGGRVVKGVQFEGLRDVGDPVELARRYEAEGADELTFLDISASAEERDTLWELVRRTAEQLFIPLAVG
GGVRTVDDVGRALRAGADKVSINSAAVANPALLTACAERFGAQCVVASIDAKRDGDRWRVYTHGGRKPTDLDAVAWAREC
VARGAGEVLLTSIDRDGARTGYDLALTRAVSEAVDVPVIASGGAGSAAHVRAAFQEGGADAALVAGILHDGVTTVGAIKA
LLRESGLHIRSLT
>Mature_253_residues
MLTRRLVVCLDVKGGRVVKGVQFEGLRDVGDPVELARRYEAEGADELTFLDISASAEERDTLWELVRRTAEQLFIPLAVG
GGVRTVDDVGRALRAGADKVSINSAAVANPALLTACAERFGAQCVVASIDAKRDGDRWRVYTHGGRKPTDLDAVAWAREC
VARGAGEVLLTSIDRDGARTGYDLALTRAVSEAVDVPVIASGGAGSAAHVRAAFQEGGADAALVAGILHDGVTTVGAIKA
LLRESGLHIRSLT

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=44.1860465116279, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082028, Length=236, Percent_Identity=29.6610169491525, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=36.741214057508, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_MYXXD (Q1D4M4)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_632399.1
- ProteinModelPortal:   Q1D4M4
- SMR:   Q1D4M4
- STRING:   Q1D4M4
- GeneID:   4105862
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_4224
- TIGR:   MXAN_4224
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q1D4M4
- ProtClustDB:   PRK02083
- BioCyc:   MXAN246197:MXAN_4224-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26569; Mature: 26569

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRLVVCLDVKGGRVVKGVQFEGLRDVGDPVELARRYEAEGADELTFLDISASAEERD
CCCCEEEEEEECCCCEEEECEECCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHH
TLWELVRRTAEQLFIPLAVGGGVRTVDDVGRALRAGADKVSINSAAVANPALLTACAERF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHCCHHHHHHHHHHH
GAQCVVASIDAKRDGDRWRVYTHGGRKPTDLDAVAWARECVARGAGEVLLTSIDRDGART
CCEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GYDLALTRAVSEAVDVPVIASGGAGSAAHVRAAFQEGGADAALVAGILHDGVTTVGAIKA
CHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
LLRESGLHIRSLT
HHHHCCCEEEECC
>Mature Secondary Structure
MLTRRLVVCLDVKGGRVVKGVQFEGLRDVGDPVELARRYEAEGADELTFLDISASAEERD
CCCCEEEEEEECCCCEEEECEECCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHH
TLWELVRRTAEQLFIPLAVGGGVRTVDDVGRALRAGADKVSINSAAVANPALLTACAERF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHCCHHHHHHHHHHH
GAQCVVASIDAKRDGDRWRVYTHGGRKPTDLDAVAWARECVARGAGEVLLTSIDRDGART
CCEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GYDLALTRAVSEAVDVPVIASGGAGSAAHVRAAFQEGGADAALVAGILHDGVTTVGAIKA
CHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
LLRESGLHIRSLT
HHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA