Click here to switch to the map view.

The map label for this gene is lpdA [H]

Identifier: 108758911

GI number: 108758911

Start: 5179304

End: 5180701

Strand: Reverse

Name: lpdA [H]

Synonym: MXAN_4219

Alternate gene names: 108758911

Gene position: 5180701-5179304 (Counterclockwise)

Preceding gene: 108760530

Following gene: 108762001

Centisome position: 56.68

GC content: 65.88

Gene sequence:

>1398_bases
GTGGCTGAGACGTTCGACGTGGTGATCATCGGTTCGGGCCCCGGCGGCTACGTGGGCGCCATTCGCGCGGGTCAGCTGGG
CCTGAAGACGGCCATCATCGAGAAGGACAAGCGGCTGGGCGGCACCTGCCTCCACCGCGGCTGCATCCCGACCAAGTCCC
TGCTGTGGACCGCGGAGCTGTTCCACCATGTCCGCGAAGCGGCTGACTTCGGCGTTGACGTGAGCAGCCCGGCCATCAAC
TGGCCCAACGCGATGAAGCACAAGGACAAGATTGTCACCAAGGGTGCCAACGGCATCGACTTCTTGATGAAGAAGAACAA
GGTGACGGTGGTGAAGGGCCACGGCCGCATCGCCGGCAAGGGCAAGGTGGAGGTCACCGCCGCGGACGGCTCCAAGCAGG
TCCTGGAGGCGAAGAACATCATCCTCGCCACGGGCTCGGTGCCCAAGTCCCTGCCCAACGTCCCCGTGGACCACAAGCGG
GTGCTGAACAGCGACTCCATCCTGCAGATCGACCGCGTCCCCAAGAGCATCATCGTGCTGGGCGCCGGCGCGGTGGGCTG
TGAGTTCGCCTCCGTGTTCAACCACGTGGGCAGCAAGACCTCCATCGTGGAGTACATGCCCGCGCTGCTCCCCATCGAGG
ACGCGGACATCTCCAAGGAGCTGGAGAAGATCTTCAAGCGTCGCGGCATCGACGTGCACACCGGCTCCGCGGTGGAGAAG
GTGGAGCACACGGCGGACGGCGTGCGCGTCACCATGAAGGTGGGCAACGAGACCAAGACGCTGGAGGCGGAAATCCTCCT
GTCGGCGGTGGGCCGCTCGCCCGTCACCGAGGACGTGGGTCTGGACAAGACGAACATCCAGGCCGAGCGCGGCTACATCA
AGGTCGACTCGATGCTGCGCACCTCCGAGCCGAACGTCTACGCCGTGGGCGACATCATCCCCACGCCGATGCTCGCGCAC
ATGGCCAGCGCGGAGTGCGTGGTGGCGGTGGAGCACATCGCCGGGAAGAACCCGCAGCCCATCAACTACGACCTGACGCC
GTCTGCCACGTACTGCTACCCCGAGGTCGCCTCGGTGGGCCTGACGGAGAAGAAGGCCAAGGAGCGCGGCTACGATGTGA
AGGTGGGCATCGCTCCGATGGGCGCCGTGACGAAGGCCTCCATCTCCAACGAGGCCACCGGCATGATCAAGATCGTGTCG
GACCGGAAGTACGACGAGGTCCTCGGCGTGCACCTCATCGGGCCGCACGCGACGGAGCTGCTGGCCGAGGCGTGTGTCGC
GCTGAAGCTGGAGATCACCACCGAGGAGCTGGCCAACACCATCCACGCGCACCCGACGCTCTCGGAAATCGTGCACGAGG
GTGCCGAGGCCACGCTGGGCCACCCGCGCCACTTCTAG

Upstream 100 bases:

>100_bases
CGGAGCGGGAAACGGCGCACGAGCAAACTCCTTGATTCCAACCGGGACCGGGGGTTATCAGCCCACCCGGGTTCCATCCG
CAATAATTACGGAGGCGTGC

Downstream 100 bases:

>100_bases
GCGGCGGCGCCTCGCTCGCAGTCCCAGGGGCCGTCCCGGTGTGCCGTGGACGGCCCCTCGTATTTCCGGCCCCCGCGGCC
GCGCGAGGTGACGGTCCCCT

Product: alpha keto acid dehydrogenase complex, E3 component, lipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MAETFDVVIIGSGPGGYVGAIRAGQLGLKTAIIEKDKRLGGTCLHRGCIPTKSLLWTAELFHHVREAADFGVDVSSPAIN
WPNAMKHKDKIVTKGANGIDFLMKKNKVTVVKGHGRIAGKGKVEVTAADGSKQVLEAKNIILATGSVPKSLPNVPVDHKR
VLNSDSILQIDRVPKSIIVLGAGAVGCEFASVFNHVGSKTSIVEYMPALLPIEDADISKELEKIFKRRGIDVHTGSAVEK
VEHTADGVRVTMKVGNETKTLEAEILLSAVGRSPVTEDVGLDKTNIQAERGYIKVDSMLRTSEPNVYAVGDIIPTPMLAH
MASAECVVAVEHIAGKNPQPINYDLTPSATYCYPEVASVGLTEKKAKERGYDVKVGIAPMGAVTKASISNEATGMIKIVS
DRKYDEVLGVHLIGPHATELLAEACVALKLEITTEELANTIHAHPTLSEIVHEGAEATLGHPRHF

Sequences:

>Translated_465_residues
MAETFDVVIIGSGPGGYVGAIRAGQLGLKTAIIEKDKRLGGTCLHRGCIPTKSLLWTAELFHHVREAADFGVDVSSPAIN
WPNAMKHKDKIVTKGANGIDFLMKKNKVTVVKGHGRIAGKGKVEVTAADGSKQVLEAKNIILATGSVPKSLPNVPVDHKR
VLNSDSILQIDRVPKSIIVLGAGAVGCEFASVFNHVGSKTSIVEYMPALLPIEDADISKELEKIFKRRGIDVHTGSAVEK
VEHTADGVRVTMKVGNETKTLEAEILLSAVGRSPVTEDVGLDKTNIQAERGYIKVDSMLRTSEPNVYAVGDIIPTPMLAH
MASAECVVAVEHIAGKNPQPINYDLTPSATYCYPEVASVGLTEKKAKERGYDVKVGIAPMGAVTKASISNEATGMIKIVS
DRKYDEVLGVHLIGPHATELLAEACVALKLEITTEELANTIHAHPTLSEIVHEGAEATLGHPRHF
>Mature_464_residues
AETFDVVIIGSGPGGYVGAIRAGQLGLKTAIIEKDKRLGGTCLHRGCIPTKSLLWTAELFHHVREAADFGVDVSSPAINW
PNAMKHKDKIVTKGANGIDFLMKKNKVTVVKGHGRIAGKGKVEVTAADGSKQVLEAKNIILATGSVPKSLPNVPVDHKRV
LNSDSILQIDRVPKSIIVLGAGAVGCEFASVFNHVGSKTSIVEYMPALLPIEDADISKELEKIFKRRGIDVHTGSAVEKV
EHTADGVRVTMKVGNETKTLEAEILLSAVGRSPVTEDVGLDKTNIQAERGYIKVDSMLRTSEPNVYAVGDIIPTPMLAHM
ASAECVVAVEHIAGKNPQPINYDLTPSATYCYPEVASVGLTEKKAKERGYDVKVGIAPMGAVTKASISNEATGMIKIVSD
RKYDEVLGVHLIGPHATELLAEACVALKLEITTEELANTIHAHPTLSEIVHEGAEATLGHPRHF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=38.8888888888889, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=28.6652078774617, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI148277065, Length=428, Percent_Identity=24.2990654205607, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI33519430, Length=428, Percent_Identity=24.2990654205607, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI33519428, Length=428, Percent_Identity=24.2990654205607, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI33519426, Length=428, Percent_Identity=24.2990654205607, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI148277071, Length=428, Percent_Identity=24.2990654205607, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI291045266, Length=429, Percent_Identity=25.8741258741259, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI22035672, Length=455, Percent_Identity=26.8131868131868, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI291045268, Length=424, Percent_Identity=24.2924528301887, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=36.8995633187773, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI87082354, Length=449, Percent_Identity=29.3986636971047, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789915, Length=438, Percent_Identity=29.9086757990868, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI87081717, Length=462, Percent_Identity=27.0562770562771, Blast_Score=167, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI32565766, Length=453, Percent_Identity=37.9690949227373, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=27.5789473684211, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71983419, Length=441, Percent_Identity=28.5714285714286, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=28.5714285714286, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71982272, Length=479, Percent_Identity=26.5135699373695, Blast_Score=118, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=39.3684210526316, Blast_Score=326, Evalue=6e-90,
Organism=Saccharomyces cerevisiae, GI6325240, Length=488, Percent_Identity=27.2540983606557, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=29.8924731182796, Blast_Score=183, Evalue=4e-47,
Organism=Drosophila melanogaster, GI21358499, Length=467, Percent_Identity=38.1156316916488, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI17737741, Length=466, Percent_Identity=27.0386266094421, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=27.3118279569892, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24640553, Length=465, Percent_Identity=27.3118279569892, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24640551, Length=465, Percent_Identity=27.3118279569892, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49674; Mature: 49543

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETFDVVIIGSGPGGYVGAIRAGQLGLKTAIIEKDKRLGGTCLHRGCIPTKSLLWTAEL
CCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHH
FHHVREAADFGVDVSSPAINWPNAMKHKDKIVTKGANGIDFLMKKNKVTVVKGHGRIAGK
HHHHHHHHHCCCCCCCCCCCCCHHHHHCHHHEECCCCCHHHEEECCEEEEEECCCEEECC
GKVEVTAADGSKQVLEAKNIILATGSVPKSLPNVPVDHKRVLNSDSILQIDRVPKSIIVL
CEEEEEECCCCHHHHHHCCEEEEECCCCCCCCCCCCCHHHHCCCCCEEEEECCCCEEEEE
GAGAVGCEFASVFNHVGSKTSIVEYMPALLPIEDADISKELEKIFKRRGIDVHTGSAVEK
ECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCHHHHH
VEHTADGVRVTMKVGNETKTLEAEILLSAVGRSPVTEDVGLDKTNIQAERGYIKVDSMLR
HHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCHHC
TSEPNVYAVGDIIPTPMLAHMASAECVVAVEHIAGKNPQPINYDLTPSATYCYPEVASVG
CCCCCEEEECCCCCCHHHHHHCCCCCEEEEHHHCCCCCCCCEEECCCCCCCCCHHHHHCC
LTEKKAKERGYDVKVGIAPMGAVTKASISNEATGMIKIVSDRKYDEVLGVHLIGPHATEL
CCHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCEEEEEECCCCCHHEEEEEEECCCHHHH
LAEACVALKLEITTEELANTIHAHPTLSEIVHEGAEATLGHPRHF
HHHHHHEEEEEECHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AETFDVVIIGSGPGGYVGAIRAGQLGLKTAIIEKDKRLGGTCLHRGCIPTKSLLWTAEL
CCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHH
FHHVREAADFGVDVSSPAINWPNAMKHKDKIVTKGANGIDFLMKKNKVTVVKGHGRIAGK
HHHHHHHHHCCCCCCCCCCCCCHHHHHCHHHEECCCCCHHHEEECCEEEEEECCCEEECC
GKVEVTAADGSKQVLEAKNIILATGSVPKSLPNVPVDHKRVLNSDSILQIDRVPKSIIVL
CEEEEEECCCCHHHHHHCCEEEEECCCCCCCCCCCCCHHHHCCCCCEEEEECCCCEEEEE
GAGAVGCEFASVFNHVGSKTSIVEYMPALLPIEDADISKELEKIFKRRGIDVHTGSAVEK
ECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCHHHHH
VEHTADGVRVTMKVGNETKTLEAEILLSAVGRSPVTEDVGLDKTNIQAERGYIKVDSMLR
HHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCHHC
TSEPNVYAVGDIIPTPMLAHMASAECVVAVEHIAGKNPQPINYDLTPSATYCYPEVASVG
CCCCCEEEECCCCCCHHHHHHCCCCCEEEEHHHCCCCCCCCEEECCCCCCCCCHHHHHCC
LTEKKAKERGYDVKVGIAPMGAVTKASISNEATGMIKIVSDRKYDEVLGVHLIGPHATEL
CCHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCEEEEEECCCCCHHEEEEEEECCCHHHH
LAEACVALKLEITTEELANTIHAHPTLSEIVHEGAEATLGHPRHF
HHHHHHEEEEEECHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]