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The map label for this gene is tgt [H]

Identifier: 108761817

GI number: 108761817

Start: 5723417

End: 5724586

Strand: Reverse

Name: tgt [H]

Synonym: MXAN_4585

Alternate gene names: 108761817

Gene position: 5724586-5723417 (Counterclockwise)

Preceding gene: 108758248

Following gene: 108759072

Centisome position: 62.63

GC content: 67.18

Gene sequence:

>1170_bases
ATGGCCGTTCGTTTCGAGCTCCTCACCACCGACCCCACGGGCGCCCGTGCAGGCATCCTGCACACACGCCGGGGTTCCTT
CAAAACCCCCATGTTCATGCCGGTGGCCACCCACGCGGGCTTCCGGCACCTGGCCATGGAGGAGGTGAAGGAGGCGGGCG
CCAGCATCCTGCTGGCCAACACCTACCATCTGATGCTCCGGCCGGGCGCCGAGGTCTTCAAGCGCTTCGGCGGCATCCAC
CCCTTCATGCAGTGGGACGGGGGCGTGCTCACCGATTCGGGCGGCTTCCAGATCTTCTCCCTGCCGGAGGACCGGCTCAT
CACGGAGAAGGGCGCCCACTTCCGCAGCTTCTACGACAACAGCCGGCAGCTCCTCAGCCCCGAGTCGAGCATCGCCATGC
AGCAGGCCATCAACTCGGAAATCATGATGGTGCTGGACGTGTGCATCGACTCGCGGACCGACGAGGCCGGCACGCGCGAG
GCCATGGAGCGCACCCACCGCTGGGCGGTGCGAAGCCTGGAGGCGAAGGCGGCGCGGGACACCGGGCAGGCGATGTTCGG
CATCGTCCAGGGCGGCGTCTTCCCTCACCTGCGTGACGAGAGCGCGGCCTTCCTGACGAAGCTGCCCTTCGACGGCTTCG
CCATTGGCGGTCTGGCCGTGGGCGAGACGAAGGTGGAGCGCGAGTCCATGACGTTGCGCGCCACCGCGTCCCTGCCCGCC
GACAAGCCGCGCTACCTGATGGGCGTGGGCACGCCCACGGACCTGGTGGAAGCCGTGTTGCGCGGTGTGGACATGTTCGA
CTGCATCATCCCCACCAAGATGGCCCAGCAGGGGTATGCGTACACGTTCAGCGGGCTCATCCGCATCTCCCGCAGCGTGT
TCCGCCTCTCCGACGAGCCGCTGGACGCGGAGTGCGACTGCTACGTGTGCAAGCGCTACACGCGCGGCTACCTGCAGCAC
CTGATGCGGGGCAAGCACCACCTGGGCTCGCGCTTCCTGTCCGTGCATAACGTCCGGCACTACCAGAAGCTGATGGGGCG
CATCCGCGAGGGCATCCTGAGTGGTACGTACGCGCAGGTGTACCGCGAGCTGAAGGCGGCCATCGCCACGCCCAAGGACT
TGAAGGACGAGGCCAACGCGCGCGAGGTGACGTTGAAGGAGGTCGGGTGA

Upstream 100 bases:

>100_bases
GGCTGTCCAGGAGGGTAGGTGGCCCCGGATGACGGGTGGGGAGCGGGCAGGGCAGATGGATGCGGTGCCTGCCGGGCATG
ACGGGTATAAGGGCCGGCCC

Downstream 100 bases:

>100_bases
GCACCGCACCCGACGCCAACCCGCGCGACGGCGACTTCGAGCTCATCACCCTGCGCAATGGCGCTCGCGCCGTGCGGCAC
CTGGGCCATGGCGAGGTGAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MAVRFELLTTDPTGARAGILHTRRGSFKTPMFMPVATHAGFRHLAMEEVKEAGASILLANTYHLMLRPGAEVFKRFGGIH
PFMQWDGGVLTDSGGFQIFSLPEDRLITEKGAHFRSFYDNSRQLLSPESSIAMQQAINSEIMMVLDVCIDSRTDEAGTRE
AMERTHRWAVRSLEAKAARDTGQAMFGIVQGGVFPHLRDESAAFLTKLPFDGFAIGGLAVGETKVERESMTLRATASLPA
DKPRYLMGVGTPTDLVEAVLRGVDMFDCIIPTKMAQQGYAYTFSGLIRISRSVFRLSDEPLDAECDCYVCKRYTRGYLQH
LMRGKHHLGSRFLSVHNVRHYQKLMGRIREGILSGTYAQVYRELKAAIATPKDLKDEANAREVTLKEVG

Sequences:

>Translated_389_residues
MAVRFELLTTDPTGARAGILHTRRGSFKTPMFMPVATHAGFRHLAMEEVKEAGASILLANTYHLMLRPGAEVFKRFGGIH
PFMQWDGGVLTDSGGFQIFSLPEDRLITEKGAHFRSFYDNSRQLLSPESSIAMQQAINSEIMMVLDVCIDSRTDEAGTRE
AMERTHRWAVRSLEAKAARDTGQAMFGIVQGGVFPHLRDESAAFLTKLPFDGFAIGGLAVGETKVERESMTLRATASLPA
DKPRYLMGVGTPTDLVEAVLRGVDMFDCIIPTKMAQQGYAYTFSGLIRISRSVFRLSDEPLDAECDCYVCKRYTRGYLQH
LMRGKHHLGSRFLSVHNVRHYQKLMGRIREGILSGTYAQVYRELKAAIATPKDLKDEANAREVTLKEVG
>Mature_388_residues
AVRFELLTTDPTGARAGILHTRRGSFKTPMFMPVATHAGFRHLAMEEVKEAGASILLANTYHLMLRPGAEVFKRFGGIHP
FMQWDGGVLTDSGGFQIFSLPEDRLITEKGAHFRSFYDNSRQLLSPESSIAMQQAINSEIMMVLDVCIDSRTDEAGTREA
MERTHRWAVRSLEAKAARDTGQAMFGIVQGGVFPHLRDESAAFLTKLPFDGFAIGGLAVGETKVERESMTLRATASLPAD
KPRYLMGVGTPTDLVEAVLRGVDMFDCIIPTKMAQQGYAYTFSGLIRISRSVFRLSDEPLDAECDCYVCKRYTRGYLQHL
MRGKHHLGSRFLSVHNVRHYQKLMGRIREGILSGTYAQVYRELKAAIATPKDLKDEANAREVTLKEVG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=354, Percent_Identity=41.2429378531073, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI13375872, Length=277, Percent_Identity=26.3537906137184, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786607, Length=353, Percent_Identity=46.742209631728, Blast_Score=340, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17544680, Length=375, Percent_Identity=38.9333333333333, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI25146502, Length=126, Percent_Identity=32.5396825396825, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920510, Length=354, Percent_Identity=44.6327683615819, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI21355311, Length=175, Percent_Identity=27.4285714285714, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43362; Mature: 43230

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRFELLTTDPTGARAGILHTRRGSFKTPMFMPVATHAGFRHLAMEEVKEAGASILLAN
CEEEEEEEECCCCCCCCCEEEECCCCCCCCEEEEEHHHCCHHHHHHHHHHHCCCEEEEEE
TYHLMLRPGAEVFKRFGGIHPFMQWDGGVLTDSGGFQIFSLPEDRLITEKGAHFRSFYDN
EEEEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHCCCHHHHHHHC
SRQLLSPESSIAMQQAINSEIMMVLDVCIDSRTDEAGTREAMERTHRWAVRSLEAKAARD
CHHHCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TGQAMFGIVQGGVFPHLRDESAAFLTKLPFDGFAIGGLAVGETKVERESMTLRATASLPA
HHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCEEECCEEECCHHHHHHHEEEEEEECCCC
DKPRYLMGVGTPTDLVEAVLRGVDMFDCIIPTKMAQQGYAYTFSGLIRISRSVFRLSDEP
CCCCEEEECCCHHHHHHHHHHCCCHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
LDAECDCYVCKRYTRGYLQHLMRGKHHLGSRFLSVHNVRHYQKLMGRIREGILSGTYAQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
YRELKAAIATPKDLKDEANAREVTLKEVG
HHHHHHHHCCCHHHHCCCCCCEEEHCCCC
>Mature Secondary Structure 
AVRFELLTTDPTGARAGILHTRRGSFKTPMFMPVATHAGFRHLAMEEVKEAGASILLAN
EEEEEEEECCCCCCCCCEEEECCCCCCCCEEEEEHHHCCHHHHHHHHHHHCCCEEEEEE
TYHLMLRPGAEVFKRFGGIHPFMQWDGGVLTDSGGFQIFSLPEDRLITEKGAHFRSFYDN
EEEEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHCCCHHHHHHHC
SRQLLSPESSIAMQQAINSEIMMVLDVCIDSRTDEAGTREAMERTHRWAVRSLEAKAARD
CHHHCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TGQAMFGIVQGGVFPHLRDESAAFLTKLPFDGFAIGGLAVGETKVERESMTLRATASLPA
HHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCEEECCEEECCHHHHHHHEEEEEEECCCC
DKPRYLMGVGTPTDLVEAVLRGVDMFDCIIPTKMAQQGYAYTFSGLIRISRSVFRLSDEP
CCCCEEEECCCHHHHHHHHHHCCCHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
LDAECDCYVCKRYTRGYLQHLMRGKHHLGSRFLSVHNVRHYQKLMGRIREGILSGTYAQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
YRELKAAIATPKDLKDEANAREVTLKEVG
HHHHHHHHCCCHHHHCCCCCCEEEHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA