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The map label for this gene is pilR [H]

Identifier: 108761773

GI number: 108761773

Start: 5213318

End: 5214685

Strand: Direct

Name: pilR [H]

Synonym: MXAN_4252

Alternate gene names: 108761773

Gene position: 5213318-5214685 (Clockwise)

Preceding gene: 108756970

Following gene: 108759035

Centisome position: 57.04

GC content: 70.39

Gene sequence:

>1368_bases
ATGACGCGCACCCGAATCCTTCTCGTGGACGACGAGCCCGGCATCCGCCTGGGCATGAGAGGCTTCCTCACCGCCCATGG
GTTCGACGTGGACGAGGCCACCAGCCTCGCCGAGGCGCAAGAGGCGTTCCGCACCTCGCGTCCCGACGTGGCCGTGGTGG
ACTACCGGCTCACGGACGGCACCGCGCTGGAGCTGCTGCCGCGCCTCAAGGACATCGACGCCTCGGTGCCCCTGGTGGTG
CTCACCGGCCATGGGTCCATCGAGCTGGCCGTCCAGGCCGTGAAGGAAGGCGCCGAGCAGTTCCTCACCAAGCCGGTGGA
ACTGGCCGTCCTCAAGGTGGTGCTGGACCGGCTGGTGTCGCAGCGCCGCGAGCGGCAGCGGCTGCTGGCGGAGCGTTCGC
GCATCCTCCGTGGGACGGTGAATCCCTTCATGGGTGGCAGCGCCACCATCCGCGCGCTCCAGGCGCAGGCCGAACGCATC
ATCGAAAGCGACAGCCCGGTGCTCATCACCGGTGAGACGGGCAGCGGCAAGAGCGTGCTCGCGCGGTGGCTGCATGAAGG
CGGCCCGCGCAAGGACGCGCCCTTCGTGGACCTCAACTGCGCGGCGCTCTCCAAGGACTTGCTCGACTCGGAGCTGTTCG
GCCACGAGAAGGGCGCCTTCACCGGCGCGGTGGCCGCCAAACAGGGCCTGCTGGAGGTCGCCGACCGAGGCACCCTCTTC
CTGGACGAAATTGGCGACATGGACTCGGCCGTCCAGCCCAAGCTCCTGAAGGTGCTGGAGGAGAAGCGCTTCCGGCGGCT
GGGCGACGTGAGGGACCGCCGCGTGGACGTGCGCCTCATCGCGGCCACGCACCAGGACCTGGCCGTGGCCGCTCGGGACA
AGCGGTTCCGCAGCGACCTCTACTTCCGCGTCAGCACGCTCATCCTCCATGTCCCCCCGCTGCGTGAGCGGCCCGAGGAC
ATCGTCGGGATGGCGCACCAATTCCTGGCAGAGCTGGGCGCGGCTCGAGGCCGCGGCAGCGTGGGGCTGCGACCCGACGC
GGAGATGGCCCTCACCTGCTACGCATGGCCTGGCAACATCCGCGAGCTGCGCAACGTGCTGGAGCGCGCGGTGCTGCTGT
CCGGCGGCGGGCCCTTGTCCCGGGGAGACTTGCGCTTCGAGGCCACCGCCGGCGAGCCCGCCTCCGACGCCGACGACCAC
CTCACCCTCGAGGAGTTGGAGCGCCGCCACATCGAGCGCATCCTCCGCCGCGAGCACGGCCACGTGGAGCGCGCCGCCGC
GCGGCTCGGCATCCCCCGGTCATCCCTCTACGAGCGGCTGAAGCGCCTGGGCATCAACCGGTCCGGATTCCAGAAACCGG
ATCCGTAG

Upstream 100 bases:

>100_bases
TCCGCTTGCGCAATCACCCGGAGGGCGGCGCGGAAGTCACACTGCTGCTTCCTGCTGTTTCTTCGCGTGTATCAACCCAG
CACGTAGACTCCCTGACTTC

Downstream 100 bases:

>100_bases
TCTGGACGCAGCAAGCAGTCTTGAGCCGTTGCGCGGGAGCAAGCACCCGAATTTGCTTGGAACCGAGACAAGCCTGTCTC
TGGCCCTGAGCTTGCTTGAG

Product: sigma-54 dependent DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTRTRILLVDDEPGIRLGMRGFLTAHGFDVDEATSLAEAQEAFRTSRPDVAVVDYRLTDGTALELLPRLKDIDASVPLVV
LTGHGSIELAVQAVKEGAEQFLTKPVELAVLKVVLDRLVSQRRERQRLLAERSRILRGTVNPFMGGSATIRALQAQAERI
IESDSPVLITGETGSGKSVLARWLHEGGPRKDAPFVDLNCAALSKDLLDSELFGHEKGAFTGAVAAKQGLLEVADRGTLF
LDEIGDMDSAVQPKLLKVLEEKRFRRLGDVRDRRVDVRLIAATHQDLAVAARDKRFRSDLYFRVSTLILHVPPLRERPED
IVGMAHQFLAELGAARGRGSVGLRPDAEMALTCYAWPGNIRELRNVLERAVLLSGGGPLSRGDLRFEATAGEPASDADDH
LTLEELERRHIERILRREHGHVERAAARLGIPRSSLYERLKRLGINRSGFQKPDP

Sequences:

>Translated_455_residues
MTRTRILLVDDEPGIRLGMRGFLTAHGFDVDEATSLAEAQEAFRTSRPDVAVVDYRLTDGTALELLPRLKDIDASVPLVV
LTGHGSIELAVQAVKEGAEQFLTKPVELAVLKVVLDRLVSQRRERQRLLAERSRILRGTVNPFMGGSATIRALQAQAERI
IESDSPVLITGETGSGKSVLARWLHEGGPRKDAPFVDLNCAALSKDLLDSELFGHEKGAFTGAVAAKQGLLEVADRGTLF
LDEIGDMDSAVQPKLLKVLEEKRFRRLGDVRDRRVDVRLIAATHQDLAVAARDKRFRSDLYFRVSTLILHVPPLRERPED
IVGMAHQFLAELGAARGRGSVGLRPDAEMALTCYAWPGNIRELRNVLERAVLLSGGGPLSRGDLRFEATAGEPASDADDH
LTLEELERRHIERILRREHGHVERAAARLGIPRSSLYERLKRLGINRSGFQKPDP
>Mature_454_residues
TRTRILLVDDEPGIRLGMRGFLTAHGFDVDEATSLAEAQEAFRTSRPDVAVVDYRLTDGTALELLPRLKDIDASVPLVVL
TGHGSIELAVQAVKEGAEQFLTKPVELAVLKVVLDRLVSQRRERQRLLAERSRILRGTVNPFMGGSATIRALQAQAERII
ESDSPVLITGETGSGKSVLARWLHEGGPRKDAPFVDLNCAALSKDLLDSELFGHEKGAFTGAVAAKQGLLEVADRGTLFL
DEIGDMDSAVQPKLLKVLEEKRFRRLGDVRDRRVDVRLIAATHQDLAVAARDKRFRSDLYFRVSTLILHVPPLRERPEDI
VGMAHQFLAELGAARGRGSVGLRPDAEMALTCYAWPGNIRELRNVLERAVLLSGGGPLSRGDLRFEATAGEPASDADDHL
TLEELERRHIERILRREHGHVERAAARLGIPRSSLYERLKRLGINRSGFQKPDP

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=36.2637362637363, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1790299, Length=471, Percent_Identity=37.791932059448, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=38.5487528344671, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1788905, Length=448, Percent_Identity=37.5, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1789087, Length=314, Percent_Identity=45.2229299363057, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI87082117, Length=316, Percent_Identity=44.9367088607595, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI87082152, Length=337, Percent_Identity=38.8724035608309, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=37.9310344827586, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787583, Length=306, Percent_Identity=34.3137254901961, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786524, Length=305, Percent_Identity=41.6393442622951, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081872, Length=270, Percent_Identity=41.1111111111111, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789828, Length=235, Percent_Identity=39.1489361702128, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=30.6666666666667, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50292; Mature: 50161

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTRILLVDDEPGIRLGMRGFLTAHGFDVDEATSLAEAQEAFRTSRPDVAVVDYRLTDG
CCCEEEEEEECCCCCEECHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
TALELLPRLKDIDASVPLVVLTGHGSIELAVQAVKEGAEQFLTKPVELAVLKVVLDRLVS
HHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QRRERQRLLAERSRILRGTVNPFMGGSATIRALQAQAERIIESDSPVLITGETGSGKSVL
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHH
ARWLHEGGPRKDAPFVDLNCAALSKDLLDSELFGHEKGAFTGAVAAKQGLLEVADRGTLF
HHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEE
LDEIGDMDSAVQPKLLKVLEEKRFRRLGDVRDRRVDVRLIAATHQDLAVAARDKRFRSDL
EHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCEEEEEEEECCCHHHHHHHHHHHHHHH
YFRVSTLILHVPPLRERPEDIVGMAHQFLAELGAARGRGSVGLRPDAEMALTCYAWPGNI
HHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCH
RELRNVLERAVLLSGGGPLSRGDLRFEATAGEPASDADDHLTLEELERRHIERILRREHG
HHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
HVERAAARLGIPRSSLYERLKRLGINRSGFQKPDP
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TRTRILLVDDEPGIRLGMRGFLTAHGFDVDEATSLAEAQEAFRTSRPDVAVVDYRLTDG
CCEEEEEEECCCCCEECHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
TALELLPRLKDIDASVPLVVLTGHGSIELAVQAVKEGAEQFLTKPVELAVLKVVLDRLVS
HHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QRRERQRLLAERSRILRGTVNPFMGGSATIRALQAQAERIIESDSPVLITGETGSGKSVL
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHH
ARWLHEGGPRKDAPFVDLNCAALSKDLLDSELFGHEKGAFTGAVAAKQGLLEVADRGTLF
HHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEE
LDEIGDMDSAVQPKLLKVLEEKRFRRLGDVRDRRVDVRLIAATHQDLAVAARDKRFRSDL
EHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCEEEEEEEECCCHHHHHHHHHHHHHHH
YFRVSTLILHVPPLRERPEDIVGMAHQFLAELGAARGRGSVGLRPDAEMALTCYAWPGNI
HHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCH
RELRNVLERAVLLSGGGPLSRGDLRFEATAGEPASDADDHLTLEELERRHIERILRREHG
HHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
HVERAAARLGIPRSSLYERLKRLGINRSGFQKPDP
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]