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The map label for this gene is gcvH

Identifier: 108761555

GI number: 108761555

Start: 3567431

End: 3567820

Strand: Direct

Name: gcvH

Synonym: MXAN_3041

Alternate gene names: 108761555

Gene position: 3567431-3567820 (Clockwise)

Preceding gene: 108758135

Following gene: 108760243

Centisome position: 39.03

GC content: 66.67

Gene sequence:

>390_bases
ATGGCCGACAACATCCCCGGCGACCTGAAGTACACCCGTGAGCACGAGTGGGCCCGCGTCCAGGGCACCTCGGTGGTGGT
GGGCGTCACGCAGCACGCGCAGGAGTCCCTGGGCGACGTGGTCTACGTGGAGCTGCCCAAGGTGGGCTCCACGGTGACGG
AGGGCAAGCAGTTCGGCGTCATCGAGTCCACCAAGGCGGTGTCGGAGCTGTACTCGCCGCTGACGGGCAAGGTGGTGAAG
GTGAACGACGGGCTGTCGGACAACCCCTCCACCGTGAACACCGACCCCTACGGTGCGGGCTGGATTGTGGAAATCGAGCC
CTCGGACCCCAAGCAGGTGGACGGACTCATGGACGCCGCCGCGTACACCGCCCTGCTCCAGAACAGCTGA

Upstream 100 bases:

>100_bases
CGACGTGGACATCCGCGGCCGCGCGGTGCCCGCAGTGGTGGTGAAGACCCCGTTCCACAAGAAGCCCTGAATCATTCCCG
TTCCAACCAGGAGCAACGAC

Downstream 100 bases:

>100_bases
GAGTGAAGGGCCGGGGACTGGTTGTTAGGGACGGTCCTCGACTTCTCGACGCACGACTCGCCTGCGAGCTACAAAGCCAT
GTCCTTGAACTGGAAGTATC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MADNIPGDLKYTREHEWARVQGTSVVVGVTQHAQESLGDVVYVELPKVGSTVTEGKQFGVIESTKAVSELYSPLTGKVVK
VNDGLSDNPSTVNTDPYGAGWIVEIEPSDPKQVDGLMDAAAYTALLQNS

Sequences:

>Translated_129_residues
MADNIPGDLKYTREHEWARVQGTSVVVGVTQHAQESLGDVVYVELPKVGSTVTEGKQFGVIESTKAVSELYSPLTGKVVK
VNDGLSDNPSTVNTDPYGAGWIVEIEPSDPKQVDGLMDAAAYTALLQNS
>Mature_128_residues
ADNIPGDLKYTREHEWARVQGTSVVVGVTQHAQESLGDVVYVELPKVGSTVTEGKQFGVIESTKAVSELYSPLTGKVVKV
NDGLSDNPSTVNTDPYGAGWIVEIEPSDPKQVDGLMDAAAYTALLQNS

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=42.8571428571429, Blast_Score=116, Evalue=4e-27,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=42.0168067226891, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=50.7936507936508, Blast_Score=147, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=37.3913043478261, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=33.0508474576271, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=44, Blast_Score=110, Evalue=8e-26,
Organism=Drosophila melanogaster, GI17865652, Length=126, Percent_Identity=38.8888888888889, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_MYXXD (Q1D7X3)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_631250.1
- ProteinModelPortal:   Q1D7X3
- SMR:   Q1D7X3
- STRING:   Q1D7X3
- GeneID:   4101105
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_3041
- TIGR:   MXAN_3041
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q1D7X3
- ProtClustDB:   PRK01202
- BioCyc:   MXAN246197:MXAN_3041-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13778; Mature: 13647

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNIPGDLKYTREHEWARVQGTSVVVGVTQHAQESLGDVVYVELPKVGSTVTEGKQFGV
CCCCCCCCCEECCCCCEEEECCCEEEEEEHHHHHHHHCCEEEEEECCCCCCCCCCCCCCC
IESTKAVSELYSPLTGKVVKVNDGLSDNPSTVNTDPYGAGWIVEIEPSDPKQVDGLMDAA
CHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
AYTALLQNS
HHHHHHCCC
>Mature Secondary Structure 
ADNIPGDLKYTREHEWARVQGTSVVVGVTQHAQESLGDVVYVELPKVGSTVTEGKQFGV
CCCCCCCCEECCCCCEEEECCCEEEEEEHHHHHHHHCCEEEEEECCCCCCCCCCCCCCC
IESTKAVSELYSPLTGKVVKVNDGLSDNPSTVNTDPYGAGWIVEIEPSDPKQVDGLMDAA
CHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
AYTALLQNS
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA