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The map label for this gene is dhaK [H]

Identifier: 108760784

GI number: 108760784

Start: 716019

End: 717719

Strand: Direct

Name: dhaK [H]

Synonym: MXAN_0618

Alternate gene names: 108760784

Gene position: 716019-717719 (Clockwise)

Preceding gene: 108758576

Following gene: 108757272

Centisome position: 7.83

GC content: 74.9

Gene sequence:

>1701_bases
ATGAAGAAGCTGGTCAACGCCCCTCGCGCGGTGGTGCGGGAGATGCTGGAGGGGTTGGTCTCGCTCGCCCCCGGGCAGGT
GCTGCTGGACGGGGAGTCGGTGGTGCTCCGCGCCGACACGCCTTCCGACGTCCGCGCGCGCAAGGTGGCTGTCATCTCCG
GTGGCGGCAGCGGCCATGAGCCGGCGCACGCGGGCTACGTGGGCGCGGGCATGCTGGACGCGGCGGTGGCCGGTGACGTC
TTCACCTCGCCCAGCACCGATGCCGTGCTGGCCGCCATCCGCGCCGTCGCGGGGCCCGCGGGCGCGCTGCTCGTCGTGAA
GAACTACACCGGGGACCGGCTCAACTTCGGGCTCGCCGCCGAGCTGGCGCGCGCCGAGGGCATCCCCGTGGAGACGGTGG
TGGTGGCGGACGACGTGTCCCTGCACGACACGGTGGAGCCCGCGCGGCGCCGGGGCATCGCTGGCACGGTGCTGGTCCAC
AAGGTCGCGGGCGCGGCGGCCGAGGCGGGCGCGGCGCTCCAGGACGTCCTCCGCGAGGCCACCGCGGCGGCGGAGGTGCT
GGGCACCATGGGCGTGGCCCTGGGGCCCTGCACCGTGCCCGCGGCGGGCAAGCCGGGCTTCACGCTGGAGGAGGACGAAA
TCGAGCTGGGCCTGGGCATCCACGGCGAGCAGGGCGTGCGGCGCGTGCCGATGCAGACGGCGGACAGCCTGGTGGACACG
CTGCTCACCACCATCGTCGAGGACCGGCGCATCACCTCGGGAGACAGGGTGGTGCTGGTGGTCAACGGATTGGGCGGCAC
GCCGCCCATGGAGCTGGCCATCGTCGCCCGGCGCGCACTGGCCGCTCTGCGTCAGGGCGGCATCCGCGTGGAGCGCGCGT
GGAGCGGGACGTTCCTCTCCGCGCTGGAGATGCCCGGCTGCTCGTTGACGCTGCTGAAGGTGGACGACGCGCGGCTGGCC
CGCCTGGATGCGGCGGTGGATGCGCCCGCGTGGCCCGGCGCGGGACGGCTGCCGAAGGAGCCGGGGGTGTACCGGCCTTC
GTCCACGGCGTCTCCAGCATCGCTTCCGGCGGAGGCGCCGCAACCGGGGATGGACCGCTTCCGGAAGGCCGCCTTGCGGG
TGGCGGACGCATTCGAGCAGTCGGAGCCCCGGCTGACCGCGCTCGATAGCGCCGCGGGCGACGGTGACCTGGGCCTCAGT
CTGGTGCGTGGCGCCGAGGCGATTCGCGCTCTTCCGGAGGACGCGTGGACGAGCCCCGCGCGTGCGCTGACGGCCATTGG
CAATGCCTTGCGGCGCAGCATTGGCGGCAGCTCGGGGCCCTTCTACGCGACGGCGCTGCTGCGCGCCGCGCGCAGGCTGG
CGGAAGGGCCCGTGGATGCCGCCGCATGGGCCGAGGCCTTCGACCTCGCCGTCACCGCCGTATCGGAGCTGGGCGGCGCG
CGGCCTGGGGACCGCACCATGCTCGATGCACTCCGGCCCGCCGCCGACGCCTTCGCGAAGGCGGTGCGTTGCGGGCAGGG
GGCTCGCGAGGCCTGGGCCTCGGCGGTGCACGCGGCCGAGGCGGGAGAGGAGGCGACGTCCCGGATGCAGCCGCGCCTGG
GACGCGCCAGCTACCTGGGTGCGCGCGCCGTCGGTGTGCCGGACGCGGGCGCCGCGGCCGTGGTGGTGTGGATGAAGGCG
CTCACGCCTGGCATCGGCTGA

Upstream 100 bases:

>100_bases
GTCTGTGCCGCGAGGAAGGCATGCTCATCGTCGGCGCCGAGTACTCGCTGGAGACGGGCGCTGTCGATTTCTTCAACGGC
GTCTGAGAGAGGACACGTTC

Downstream 100 bases:

>100_bases
GCGCCGTCGGGATTCGGGCCTTCCGGCCTACTGCACCCAGCGTTTGCTGGTCTGGTACACGTCCACCAGGGGGGAGACCA
GGGTGAGGCGCGGCCCCCAA

Product: dihydroxyacetone kinase

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKKLVNAPRAVVREMLEGLVSLAPGQVLLDGESVVLRADTPSDVRARKVAVISGGGSGHEPAHAGYVGAGMLDAAVAGDV
FTSPSTDAVLAAIRAVAGPAGALLVVKNYTGDRLNFGLAAELARAEGIPVETVVVADDVSLHDTVEPARRRGIAGTVLVH
KVAGAAAEAGAALQDVLREATAAAEVLGTMGVALGPCTVPAAGKPGFTLEEDEIELGLGIHGEQGVRRVPMQTADSLVDT
LLTTIVEDRRITSGDRVVLVVNGLGGTPPMELAIVARRALAALRQGGIRVERAWSGTFLSALEMPGCSLTLLKVDDARLA
RLDAAVDAPAWPGAGRLPKEPGVYRPSSTASPASLPAEAPQPGMDRFRKAALRVADAFEQSEPRLTALDSAAGDGDLGLS
LVRGAEAIRALPEDAWTSPARALTAIGNALRRSIGGSSGPFYATALLRAARRLAEGPVDAAAWAEAFDLAVTAVSELGGA
RPGDRTMLDALRPAADAFAKAVRCGQGAREAWASAVHAAEAGEEATSRMQPRLGRASYLGARAVGVPDAGAAAVVVWMKA
LTPGIG

Sequences:

>Translated_566_residues
MKKLVNAPRAVVREMLEGLVSLAPGQVLLDGESVVLRADTPSDVRARKVAVISGGGSGHEPAHAGYVGAGMLDAAVAGDV
FTSPSTDAVLAAIRAVAGPAGALLVVKNYTGDRLNFGLAAELARAEGIPVETVVVADDVSLHDTVEPARRRGIAGTVLVH
KVAGAAAEAGAALQDVLREATAAAEVLGTMGVALGPCTVPAAGKPGFTLEEDEIELGLGIHGEQGVRRVPMQTADSLVDT
LLTTIVEDRRITSGDRVVLVVNGLGGTPPMELAIVARRALAALRQGGIRVERAWSGTFLSALEMPGCSLTLLKVDDARLA
RLDAAVDAPAWPGAGRLPKEPGVYRPSSTASPASLPAEAPQPGMDRFRKAALRVADAFEQSEPRLTALDSAAGDGDLGLS
LVRGAEAIRALPEDAWTSPARALTAIGNALRRSIGGSSGPFYATALLRAARRLAEGPVDAAAWAEAFDLAVTAVSELGGA
RPGDRTMLDALRPAADAFAKAVRCGQGAREAWASAVHAAEAGEEATSRMQPRLGRASYLGARAVGVPDAGAAAVVVWMKA
LTPGIG
>Mature_566_residues
MKKLVNAPRAVVREMLEGLVSLAPGQVLLDGESVVLRADTPSDVRARKVAVISGGGSGHEPAHAGYVGAGMLDAAVAGDV
FTSPSTDAVLAAIRAVAGPAGALLVVKNYTGDRLNFGLAAELARAEGIPVETVVVADDVSLHDTVEPARRRGIAGTVLVH
KVAGAAAEAGAALQDVLREATAAAEVLGTMGVALGPCTVPAAGKPGFTLEEDEIELGLGIHGEQGVRRVPMQTADSLVDT
LLTTIVEDRRITSGDRVVLVVNGLGGTPPMELAIVARRALAALRQGGIRVERAWSGTFLSALEMPGCSLTLLKVDDARLA
RLDAAVDAPAWPGAGRLPKEPGVYRPSSTASPASLPAEAPQPGMDRFRKAALRVADAFEQSEPRLTALDSAAGDGDLGLS
LVRGAEAIRALPEDAWTSPARALTAIGNALRRSIGGSSGPFYATALLRAARRLAEGPVDAAAWAEAFDLAVTAVSELGGA
RPGDRTMLDALRPAADAFAKAVRCGQGAREAWASAVHAAEAGEEATSRMQPRLGRASYLGARAVGVPDAGAAAVVVWMKA
LTPGIG

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=564, Percent_Identity=45.9219858156028, Blast_Score=403, Evalue=1e-112,
Organism=Escherichia coli, GI87081857, Length=354, Percent_Identity=44.9152542372881, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1787449, Length=191, Percent_Identity=34.0314136125654, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17565018, Length=575, Percent_Identity=37.7391304347826, Blast_Score=330, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6321055, Length=573, Percent_Identity=36.3001745200698, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6323570, Length=588, Percent_Identity=35.0340136054422, Blast_Score=270, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 57839; Mature: 57839

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVNAPRAVVREMLEGLVSLAPGQVLLDGESVVLRADTPSDVRARKVAVISGGGSGHE
CCHHHCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCEEEEEEEECCCCCCC
PAHAGYVGAGMLDAAVAGDVFTSPSTDAVLAAIRAVAGPAGALLVVKNYTGDRLNFGLAA
CCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHH
ELARAEGIPVETVVVADDVSLHDTVEPARRRGIAGTVLVHKVAGAAAEAGAALQDVLREA
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TAAAEVLGTMGVALGPCTVPAAGKPGFTLEEDEIELGLGIHGEQGVRRVPMQTADSLVDT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
LLTTIVEDRRITSGDRVVLVVNGLGGTPPMELAIVARRALAALRQGGIRVERAWSGTFLS
HHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHH
ALEMPGCSLTLLKVDDARLARLDAAVDAPAWPGAGRLPKEPGVYRPSSTASPASLPAEAP
HHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QPGMDRFRKAALRVADAFEQSEPRLTALDSAAGDGDLGLSLVRGAEAIRALPEDAWTSPA
CCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHCCCHH
RALTAIGNALRRSIGGSSGPFYATALLRAARRLAEGPVDAAAWAEAFDLAVTAVSELGGA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
RPGDRTMLDALRPAADAFAKAVRCGQGAREAWASAVHAAEAGEEATSRMQPRLGRASYLG
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHCC
ARAVGVPDAGAAAVVVWMKALTPGIG
CCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKLVNAPRAVVREMLEGLVSLAPGQVLLDGESVVLRADTPSDVRARKVAVISGGGSGHE
CCHHHCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCEEEEEEEECCCCCCC
PAHAGYVGAGMLDAAVAGDVFTSPSTDAVLAAIRAVAGPAGALLVVKNYTGDRLNFGLAA
CCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHH
ELARAEGIPVETVVVADDVSLHDTVEPARRRGIAGTVLVHKVAGAAAEAGAALQDVLREA
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TAAAEVLGTMGVALGPCTVPAAGKPGFTLEEDEIELGLGIHGEQGVRRVPMQTADSLVDT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
LLTTIVEDRRITSGDRVVLVVNGLGGTPPMELAIVARRALAALRQGGIRVERAWSGTFLS
HHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHH
ALEMPGCSLTLLKVDDARLARLDAAVDAPAWPGAGRLPKEPGVYRPSSTASPASLPAEAP
HHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QPGMDRFRKAALRVADAFEQSEPRLTALDSAAGDGDLGLSLVRGAEAIRALPEDAWTSPA
CCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHCCCHH
RALTAIGNALRRSIGGSSGPFYATALLRAARRLAEGPVDAAAWAEAFDLAVTAVSELGGA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
RPGDRTMLDALRPAADAFAKAVRCGQGAREAWASAVHAAEAGEEATSRMQPRLGRASYLG
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHCC
ARAVGVPDAGAAAVVVWMKALTPGIG
CCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]