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The map label for this gene is gmd [H]

Identifier: 108760364

GI number: 108760364

Start: 6633484

End: 6634458

Strand: Direct

Name: gmd [H]

Synonym: MXAN_5327

Alternate gene names: 108760364

Gene position: 6633484-6634458 (Clockwise)

Preceding gene: 108763729

Following gene: 108758739

Centisome position: 72.58

GC content: 65.85

Gene sequence:

>975_bases
ATGGCGACCAAGCGCGCACTCATTACGGGCATCACGGGGCAGGACGGCAGCTATCTCGCGGAGCTGCTCCTTTCGAAGGG
CTACGAGGTGCACGGCATGGTGCGCCGCTCGTCGGAGGAGAAGTTCGAGCGCATCCAGCACCTGCACGGGAAGATTCAGC
TTCACCAGGGCGACTTGCTGGACCAGTTCTCGCTCGCGGCGTTGCTCAACCTGACGAAGCCCGACGAGGTCTACAACTTG
GCGGCGCAGTCCTTCGTCCCCACCAGTTGGAACCAGCCGGTGCTCACGGGTGAGTTCACCGCGCTGGGCGTGACGAAGAT
GCTGGAGGCCATCCGCCACACCCGCCCGGAGGCGCGCTTCTACCAGGCGTCGTCCAGCGAGATGTTCGGCAAGGTGCTGG
AAGTTCCGCAGACGGAGGACACGCCCTTCTATCCGCGCAGCCCGTACGGTGTGGCCAAGGCCTACGGCCACCACATCACG
GTGAACTACCGCGAGTCCTTCAACCTGTTCGCGGTGAGCGGCATCCTCTTCAACCACGAGTCGCCGCGCCGGGGCCTGGA
GTTCGTCACGCGCAAGGTCACCTACAACGTGGCGCGCATCAAGCTGGGCCTCCAGGAGAAGCTGCCCATGGGCAACCTGG
ACGCCAAGCGTGACTGGGGCTTCGCGGGCGACTACGTGGACGCCATGTGGCGGATGCTCCAGCAGCCGCAGGCCGAGGAC
TACGTGGTGGCCACCAACGAGACGCACACCGTGCGCGAGCTGGTGGAGATTGCCTTCGCGCGCGTGGGCCTGGACTGGGA
GAAGCACGTCTTCATCGACCCGGCCTTCGTGCGCCCCGCCGAGGTGGACCTGCTCATCGGTGACCCGGCCAAGGCGAAGA
CGAAGCTGGGCTGGGAGCCCAAGGTCCGCTTCAAGGAGCTGGTGGAGATGATGGTGGACGCGGACCTGGAGCGCGTGAAG
GCGGGGCAGCGGTAG

Upstream 100 bases:

>100_bases
CGAGGCGTCAGGCTTTCAAGTAGGAAGCGGCCGGGAACGGTTGGCGATGAGTGACAGCGTCGGCTAGAAAGTGCGTCTTC
CAGCCAACACAGAAAAGAAC

Downstream 100 bases:

>100_bases
ATGCGCATTCTGGTAACAGGCGCGGATGGCTTCGTCGGCCGGCACTTGTGCGCCCTGCTGCGCGCCGCCGGCGACGAGGT
AGTGGAGGCCCACGGGCCGC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MATKRALITGITGQDGSYLAELLLSKGYEVHGMVRRSSEEKFERIQHLHGKIQLHQGDLLDQFSLAALLNLTKPDEVYNL
AAQSFVPTSWNQPVLTGEFTALGVTKMLEAIRHTRPEARFYQASSSEMFGKVLEVPQTEDTPFYPRSPYGVAKAYGHHIT
VNYRESFNLFAVSGILFNHESPRRGLEFVTRKVTYNVARIKLGLQEKLPMGNLDAKRDWGFAGDYVDAMWRMLQQPQAED
YVVATNETHTVRELVEIAFARVGLDWEKHVFIDPAFVRPAEVDLLIGDPAKAKTKLGWEPKVRFKELVEMMVDADLERVK
AGQR

Sequences:

>Translated_324_residues
MATKRALITGITGQDGSYLAELLLSKGYEVHGMVRRSSEEKFERIQHLHGKIQLHQGDLLDQFSLAALLNLTKPDEVYNL
AAQSFVPTSWNQPVLTGEFTALGVTKMLEAIRHTRPEARFYQASSSEMFGKVLEVPQTEDTPFYPRSPYGVAKAYGHHIT
VNYRESFNLFAVSGILFNHESPRRGLEFVTRKVTYNVARIKLGLQEKLPMGNLDAKRDWGFAGDYVDAMWRMLQQPQAED
YVVATNETHTVRELVEIAFARVGLDWEKHVFIDPAFVRPAEVDLLIGDPAKAKTKLGWEPKVRFKELVEMMVDADLERVK
AGQR
>Mature_323_residues
ATKRALITGITGQDGSYLAELLLSKGYEVHGMVRRSSEEKFERIQHLHGKIQLHQGDLLDQFSLAALLNLTKPDEVYNLA
AQSFVPTSWNQPVLTGEFTALGVTKMLEAIRHTRPEARFYQASSSEMFGKVLEVPQTEDTPFYPRSPYGVAKAYGHHITV
NYRESFNLFAVSGILFNHESPRRGLEFVTRKVTYNVARIKLGLQEKLPMGNLDAKRDWGFAGDYVDAMWRMLQQPQAEDY
VVATNETHTVRELVEIAFARVGLDWEKHVFIDPAFVRPAEVDLLIGDPAKAKTKLGWEPKVRFKELVEMMVDADLERVKA
GQR

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=53.2163742690059, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI42516563, Length=314, Percent_Identity=25.1592356687898, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788366, Length=354, Percent_Identity=50, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1788353, Length=292, Percent_Identity=24.6575342465753, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994969, Length=184, Percent_Identity=27.7173913043478, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=345, Percent_Identity=52.1739130434783, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI133901790, Length=347, Percent_Identity=52.4495677233429, Blast_Score=354, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI133901788, Length=347, Percent_Identity=52.4495677233429, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI17539422, Length=347, Percent_Identity=52.4495677233429, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI17539424, Length=347, Percent_Identity=52.4495677233429, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI133901786, Length=347, Percent_Identity=52.4495677233429, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=25.8823529411765, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24158427, Length=341, Percent_Identity=53.0791788856305, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI21356223, Length=310, Percent_Identity=25.1612903225806, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 36783; Mature: 36651

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKRALITGITGQDGSYLAELLLSKGYEVHGMVRRSSEEKFERIQHLHGKIQLHQGDLL
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHHHCEEEEECCCCH
DQFSLAALLNLTKPDEVYNLAAQSFVPTSWNQPVLTGEFTALGVTKMLEAIRHTRPEARF
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHE
YQASSSEMFGKVLEVPQTEDTPFYPRSPYGVAKAYGHHITVNYRESFNLFAVSGILFNHE
EECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCEEEEEEEEECCC
SPRRGLEFVTRKVTYNVARIKLGLQEKLPMGNLDAKRDWGFAGDYVDAMWRMLQQPQAED
CCHHHHHHHHHHHHHEEEHEEECHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
YVVATNETHTVRELVEIAFARVGLDWEKHVFIDPAFVRPAEVDLLIGDPAKAKTKLGWEP
EEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCHHCCCCCCC
KVRFKELVEMMVDADLERVKAGQR
CHHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
ATKRALITGITGQDGSYLAELLLSKGYEVHGMVRRSSEEKFERIQHLHGKIQLHQGDLL
CCCCEEEEECCCCCHHHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHHHCEEEEECCCCH
DQFSLAALLNLTKPDEVYNLAAQSFVPTSWNQPVLTGEFTALGVTKMLEAIRHTRPEARF
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHE
YQASSSEMFGKVLEVPQTEDTPFYPRSPYGVAKAYGHHITVNYRESFNLFAVSGILFNHE
EECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCEEEEEEEEECCC
SPRRGLEFVTRKVTYNVARIKLGLQEKLPMGNLDAKRDWGFAGDYVDAMWRMLQQPQAED
CCHHHHHHHHHHHHHEEEHEEECHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
YVVATNETHTVRELVEIAFARVGLDWEKHVFIDPAFVRPAEVDLLIGDPAKAKTKLGWEP
EEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCHHCCCCCCC
KVRFKELVEMMVDADLERVKAGQR
CHHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]