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The map label for this gene is sasR [H]

Identifier: 108760029

GI number: 108760029

Start: 1456721

End: 1458154

Strand: Reverse

Name: sasR [H]

Synonym: MXAN_1245

Alternate gene names: 108760029

Gene position: 1458154-1456721 (Counterclockwise)

Preceding gene: 108758193

Following gene: 108759167

Centisome position: 15.95

GC content: 68.41

Gene sequence:

>1434_bases
ATGCAGGGAGAATCGACTCAAGCCATGGCGCGAATCCTCGTCATCGACGACCACGACACGCTGCGTGAAGGGATGGCTGT
CACCCTCACTCGGGCAGGCCACACCGTGTCCGCGGTGCGCAGTGGCCAGGACGGATTGGCCGCCTACCGCAAGGCACCGT
TCGACCTGGTCGTCACCGACCTGAAGATGGACGGTATGGACGGCATCGAGGTGACGAAGTCGCTCAAGGCGCTGGATGCC
GGCGCCGTCGTCATGGTGGTGACGGCCTTCGGCACCATCGAGACCGCGGTGCAGGCGATGCAGCAAGGCGCCTACGACTT
CATCACCAAGCCCTTCCCGCCCGAGGTGCTGCGCGCCAAGGTGGACAAGGGGCTGGAGCTGGCCTCCACGCGCAAGCAGG
TGGAGCGACTGACGGCGCGCACCGCCGCGCACGACACGGACGCGGCCCTCACGCACGGCAGCCTGGTGGGCGACAGCGAG
CCCATGCAGCGGCTGGTGTCCCAGGTGCTCAAGGTGGCGCAGAGCGACGCCACGGTGCTGGTGCGCGGAGAGAGCGGCAC
CGGCAAGGAGCTGGTGGCACGGATGCTCCACCAGCGCTCCCCGCGCCGAGACGGCCCCTTCATCGTCGTGCACTGCGCGG
CGCTGGCGGAGACCCTGCTGGAGAGCGAGCTGTTCGGGCACGAGCGCGGCGCCTTCACCGGCGCGGTGAAGCGCAAGCTG
GGGCGCTTCGAGCTGGCCGACGGCGGCACGCTGTTCCTGGACGAGATTGGCGAGATTCCCGCCTCTGTGCAGACGAAGCT
CTTGCGCGTGCTCCAGGAGAAGGAAATCCAGCGCGTCGGCGGAGAGGACACGTTCAAGGTGGACGTGCGCGTGGTGAGCG
CCACCCACCGGGACCTCCAGGCCGAGGTGAAGGCCGGACGCTTCCGCGAGGACCTCTACTACCGCCTGCACATCGTTCCG
GTGGTACTGCCGCCCCTGCGCGAGCGTCCCGAGGACATCTCCCTGCTGGCCCGACACTTCGTGGCCAAGCACGCCATGCG
CGTGAACCGACGTGTGATGGGCTTGGACGACAGCGCGCTGCGCGCCCTGGCCCGCCACGCGTGGCCCGGCAACGTGCGCG
AGCTGGAGAACGTCATCGAACAGGCGCTCGTCTTCGCCGAAGGTGAGCAGCTCACCGACCTGGACCTGCCCTCACACCTC
ACCGCCAGTGGCACCCCGCGCGTCGAGGCGGGCCTGCCAGTGCTCCAGGGCGACCGCCCCCTGCCCGACATCCTGGAGGA
TTTGGAGCGTCAGCTCATCGCCCGGGCCTATGAGAAGGCCAGCGGCGTGAAGACGGAGACGGCCCGCCTGCTAGGTATCA
AGACGTCCGCGCTGTACTACAAGCTGGAGAAGTACGGCTTCCTCCCCAAGGGGACGCCTCCCGAGGAGAGCTGA

Upstream 100 bases:

>100_bases
CTTCTCACGCCCGTGACATGTGACAACCTCCTGAGCCGGTGGAGTGAGCCGGTCCGGATGTGCTCTCATCCGGCGGCCCC
GGGAGGCGGCCGGCCTCGGG

Downstream 100 bases:

>100_bases
ACCTCGAAAATCTGTCATCCGCCCCCGCCCCCCATGACTTTTCACCGGTATCCCACCTCCGCCCCTGTCCCCCTGGATGC
TCCAGGTGTCCGGAATTGTT

Product: sigma-54 dependent DNA-binding response regulator SasR

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MQGESTQAMARILVIDDHDTLREGMAVTLTRAGHTVSAVRSGQDGLAAYRKAPFDLVVTDLKMDGMDGIEVTKSLKALDA
GAVVMVVTAFGTIETAVQAMQQGAYDFITKPFPPEVLRAKVDKGLELASTRKQVERLTARTAAHDTDAALTHGSLVGDSE
PMQRLVSQVLKVAQSDATVLVRGESGTGKELVARMLHQRSPRRDGPFIVVHCAALAETLLESELFGHERGAFTGAVKRKL
GRFELADGGTLFLDEIGEIPASVQTKLLRVLQEKEIQRVGGEDTFKVDVRVVSATHRDLQAEVKAGRFREDLYYRLHIVP
VVLPPLRERPEDISLLARHFVAKHAMRVNRRVMGLDDSALRALARHAWPGNVRELENVIEQALVFAEGEQLTDLDLPSHL
TASGTPRVEAGLPVLQGDRPLPDILEDLERQLIARAYEKASGVKTETARLLGIKTSALYYKLEKYGFLPKGTPPEES

Sequences:

>Translated_477_residues
MQGESTQAMARILVIDDHDTLREGMAVTLTRAGHTVSAVRSGQDGLAAYRKAPFDLVVTDLKMDGMDGIEVTKSLKALDA
GAVVMVVTAFGTIETAVQAMQQGAYDFITKPFPPEVLRAKVDKGLELASTRKQVERLTARTAAHDTDAALTHGSLVGDSE
PMQRLVSQVLKVAQSDATVLVRGESGTGKELVARMLHQRSPRRDGPFIVVHCAALAETLLESELFGHERGAFTGAVKRKL
GRFELADGGTLFLDEIGEIPASVQTKLLRVLQEKEIQRVGGEDTFKVDVRVVSATHRDLQAEVKAGRFREDLYYRLHIVP
VVLPPLRERPEDISLLARHFVAKHAMRVNRRVMGLDDSALRALARHAWPGNVRELENVIEQALVFAEGEQLTDLDLPSHL
TASGTPRVEAGLPVLQGDRPLPDILEDLERQLIARAYEKASGVKTETARLLGIKTSALYYKLEKYGFLPKGTPPEES
>Mature_477_residues
MQGESTQAMARILVIDDHDTLREGMAVTLTRAGHTVSAVRSGQDGLAAYRKAPFDLVVTDLKMDGMDGIEVTKSLKALDA
GAVVMVVTAFGTIETAVQAMQQGAYDFITKPFPPEVLRAKVDKGLELASTRKQVERLTARTAAHDTDAALTHGSLVGDSE
PMQRLVSQVLKVAQSDATVLVRGESGTGKELVARMLHQRSPRRDGPFIVVHCAALAETLLESELFGHERGAFTGAVKRKL
GRFELADGGTLFLDEIGEIPASVQTKLLRVLQEKEIQRVGGEDTFKVDVRVVSATHRDLQAEVKAGRFREDLYYRLHIVP
VVLPPLRERPEDISLLARHFVAKHAMRVNRRVMGLDDSALRALARHAWPGNVRELENVIEQALVFAEGEQLTDLDLPSHL
TASGTPRVEAGLPVLQGDRPLPDILEDLERQLIARAYEKASGVKTETARLLGIKTSALYYKLEKYGFLPKGTPPEES

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=466, Percent_Identity=43.3476394849785, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=46.0526315789474, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1790299, Length=477, Percent_Identity=40.251572327044, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1788905, Length=456, Percent_Identity=39.9122807017544, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI87082117, Length=329, Percent_Identity=47.7203647416413, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789087, Length=355, Percent_Identity=44.5070422535211, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=43.125, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87082152, Length=327, Percent_Identity=42.5076452599388, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786524, Length=350, Percent_Identity=41.4285714285714, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI87081872, Length=243, Percent_Identity=49.7942386831276, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1787583, Length=339, Percent_Identity=38.9380530973451, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87081858, Length=318, Percent_Identity=33.0188679245283, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1789828, Length=241, Percent_Identity=37.7593360995851, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87082012, Length=111, Percent_Identity=31.5315315315315, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786784, Length=111, Percent_Identity=28.8288288288288, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789402, Length=157, Percent_Identity=27.3885350318471, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52293; Mature: 52293

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGESTQAMARILVIDDHDTLREGMAVTLTRAGHTVSAVRSGQDGLAAYRKAPFDLVVTD
CCCCCCCCEEEEEEECCCHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHCCCCEEEEEE
LKMDGMDGIEVTKSLKALDAGAVVMVVTAFGTIETAVQAMQQGAYDFITKPFPPEVLRAK
EEECCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHH
VDKGLELASTRKQVERLTARTAAHDTDAALTHGSLVGDSEPMQRLVSQVLKVAQSDATVL
HHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
VRGESGTGKELVARMLHQRSPRRDGPFIVVHCAALAETLLESELFGHERGAFTGAVKRKL
EECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
GRFELADGGTLFLDEIGEIPASVQTKLLRVLQEKEIQRVGGEDTFKVDVRVVSATHRDLQ
CCEEECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHH
AEVKAGRFREDLYYRLHIVPVVLPPLRERPEDISLLARHFVAKHAMRVNRRVMGLDDSAL
HHHHHHHHHHHHEEEEEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RALARHAWPGNVRELENVIEQALVFAEGEQLTDLDLPSHLTASGTPRVEAGLPVLQGDRP
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LPDILEDLERQLIARAYEKASGVKTETARLLGIKTSALYYKLEKYGFLPKGTPPEES
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQGESTQAMARILVIDDHDTLREGMAVTLTRAGHTVSAVRSGQDGLAAYRKAPFDLVVTD
CCCCCCCCEEEEEEECCCHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHCCCCEEEEEE
LKMDGMDGIEVTKSLKALDAGAVVMVVTAFGTIETAVQAMQQGAYDFITKPFPPEVLRAK
EEECCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHH
VDKGLELASTRKQVERLTARTAAHDTDAALTHGSLVGDSEPMQRLVSQVLKVAQSDATVL
HHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
VRGESGTGKELVARMLHQRSPRRDGPFIVVHCAALAETLLESELFGHERGAFTGAVKRKL
EECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
GRFELADGGTLFLDEIGEIPASVQTKLLRVLQEKEIQRVGGEDTFKVDVRVVSATHRDLQ
CCEEECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHH
AEVKAGRFREDLYYRLHIVPVVLPPLRERPEDISLLARHFVAKHAMRVNRRVMGLDDSAL
HHHHHHHHHHHHEEEEEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RALARHAWPGNVRELENVIEQALVFAEGEQLTDLDLPSHLTASGTPRVEAGLPVLQGDRP
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LPDILEDLERQLIARAYEKASGVKTETARLLGIKTSALYYKLEKYGFLPKGTPPEES
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]