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The map label for this gene is sasS [H]

Identifier: 108758193

GI number: 108758193

Start: 1461842

End: 1463335

Strand: Reverse

Name: sasS [H]

Synonym: MXAN_1249

Alternate gene names: 108758193

Gene position: 1463335-1461842 (Counterclockwise)

Preceding gene: 108758151

Following gene: 108760029

Centisome position: 16.01

GC content: 72.09

Gene sequence:

>1494_bases
ATGCCCCACGTTGCCCCACACACTTCGCGATGGCGTGACGCGCTGGGCTCGCTCTCCGCGCGACTACTGGTGGCGTTCCT
CGTGCCCACCCTGCTCTTCGTCGCGGTGGCGGGCACCGCCGTCTACCAGCTCGCGCGCGTCATCCTGGAAGAAGAGCTGG
GCACCAGCCTCAGCGGCATCGCCGCCGCCACCGCCACCCAGGTCCATGGCGAGCGGCTGCTGACCATCGAACCCGGCGAC
GACGTGGCAAGCACCCGCACGTGGCGCAACTTCGTCCGCACCTTCGAGAACATCGCGGAGGCCAGTGGCGTGCGCCGCGT
CTACGCCGTGGACACGCAGGGCCGGATGCGCGTGGACGTCGGGGGCGGGCTCCCGGTGGGCGCGGAGATGCCGGAGCTGG
CGCGCGACAGGCTGGAGCTCACCCGTGTCTTCGCCGGGGAGCGCGCCGCCAGTCAGGTGCTCTTCACCGGCTCGGACGGA
CAGCGCTACAAGACGGGGTATGCGCCCATCCTGTACGAGGGCCGCGTGGTGGGCGCCGTGGGAGTGGAGGCCAGCGCCGC
CTTCTTCGGTCCCCTGCAGCGACTGTCACGCACCTACGCCATCATGGGCGCGGTGGCGCTGAGCGCGCTCGCCGTCATCG
CGTTGGTGACGGCGCGCGGTCTGGCCCGGCCGCTGCGGCGGCTGATGGCGTCCGCGCTGCGAATCGGCCGGGGCGACCTG
ACGACCCCGGTGCCACCGGAGCCCACGCTGGAAATCGGCGTCCTCGCGCGCGAGCTGGAGGACATGCGCGGCGCCCTGGA
GAGCCGGGACCGTCAGTTGAAGATGATGCTCGCGGGCGTGGCCCACGAGGTGCGCAACCCCATTGGCGGCATCGCGCTCT
TCTCCGGAATCTTGAAGGAGGACCTCCAGGCCGGCGCCCACGCCGACGCGGGCGCGCACGTGGAGCGCATCCAACGCGAG
GTCGCCTACCTCCAGCGCATCGTCGAGGACTTCCTCGCCTTCGCCCGCGAGCAGCCCCTGGCCCGCGCGCCCGTGGAGGC
CCCCACGCTGCTCTCCGTCGCCTGCGAGCTGATGGCCGCCGAGGCCAGCGCCCGCGACGTCGTGGTGGACATCGACGCCT
CCCCCGCGGTGCTGGAAGCTGACGGCAGCCTGCTCACCGCGGCGCTGGTCAACCTGGTGAAGAACGCGGTGCAGGCTTCC
CCCCCGGGTGGGCGCGTACAGGTGTCCGGCGCGTCCAAAGACGGCCACTACACCATCCAGGTCCGCGACACGGGCCCCGG
CGTGCCCGGCACGGCGCGCGAGCGCATCTTCGAGCCCTTCTTCACCACGCGCGAGAAGGGCACCGGCCTGGGCCTGCCAC
TGGCGCACAAGATTGCGCTCGCGCACGGCGGCGCGCTGCGGCTGGACTCCGCGCCTGGAGACACGCGCTTCATCCTCACG
CTGCCGTTGGGTGGAAAGCCGGGCCACGGCGTATCCGTCACCCCGTCGCGTTGA

Upstream 100 bases:

>100_bases
CACACGAAGGCGCCCTCGGCAACCCCCATCCGAGCAGGCAGCAGCACACAGCCGCGCCCCCGCGAGGGAGACGAGGCTCG
ATTAGAGTCCCGCCCCGGAC

Downstream 100 bases:

>100_bases
ACTGCCGCGCCATCCGCGCCACGTCGTCGGAGACGACCCTCAGCGTGTCGGTGGCCTCCTGTGTGGACTCCAACCGCTTC
AGCGTGGCGTCCATGAGCTG

Product: developmental sensor histidine kinase SasS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MPHVAPHTSRWRDALGSLSARLLVAFLVPTLLFVAVAGTAVYQLARVILEEELGTSLSGIAAATATQVHGERLLTIEPGD
DVASTRTWRNFVRTFENIAEASGVRRVYAVDTQGRMRVDVGGGLPVGAEMPELARDRLELTRVFAGERAASQVLFTGSDG
QRYKTGYAPILYEGRVVGAVGVEASAAFFGPLQRLSRTYAIMGAVALSALAVIALVTARGLARPLRRLMASALRIGRGDL
TTPVPPEPTLEIGVLARELEDMRGALESRDRQLKMMLAGVAHEVRNPIGGIALFSGILKEDLQAGAHADAGAHVERIQRE
VAYLQRIVEDFLAFAREQPLARAPVEAPTLLSVACELMAAEASARDVVVDIDASPAVLEADGSLLTAALVNLVKNAVQAS
PPGGRVQVSGASKDGHYTIQVRDTGPGVPGTARERIFEPFFTTREKGTGLGLPLAHKIALAHGGALRLDSAPGDTRFILT
LPLGGKPGHGVSVTPSR

Sequences:

>Translated_497_residues
MPHVAPHTSRWRDALGSLSARLLVAFLVPTLLFVAVAGTAVYQLARVILEEELGTSLSGIAAATATQVHGERLLTIEPGD
DVASTRTWRNFVRTFENIAEASGVRRVYAVDTQGRMRVDVGGGLPVGAEMPELARDRLELTRVFAGERAASQVLFTGSDG
QRYKTGYAPILYEGRVVGAVGVEASAAFFGPLQRLSRTYAIMGAVALSALAVIALVTARGLARPLRRLMASALRIGRGDL
TTPVPPEPTLEIGVLARELEDMRGALESRDRQLKMMLAGVAHEVRNPIGGIALFSGILKEDLQAGAHADAGAHVERIQRE
VAYLQRIVEDFLAFAREQPLARAPVEAPTLLSVACELMAAEASARDVVVDIDASPAVLEADGSLLTAALVNLVKNAVQAS
PPGGRVQVSGASKDGHYTIQVRDTGPGVPGTARERIFEPFFTTREKGTGLGLPLAHKIALAHGGALRLDSAPGDTRFILT
LPLGGKPGHGVSVTPSR
>Mature_496_residues
PHVAPHTSRWRDALGSLSARLLVAFLVPTLLFVAVAGTAVYQLARVILEEELGTSLSGIAAATATQVHGERLLTIEPGDD
VASTRTWRNFVRTFENIAEASGVRRVYAVDTQGRMRVDVGGGLPVGAEMPELARDRLELTRVFAGERAASQVLFTGSDGQ
RYKTGYAPILYEGRVVGAVGVEASAAFFGPLQRLSRTYAIMGAVALSALAVIALVTARGLARPLRRLMASALRIGRGDLT
TPVPPEPTLEIGVLARELEDMRGALESRDRQLKMMLAGVAHEVRNPIGGIALFSGILKEDLQAGAHADAGAHVERIQREV
AYLQRIVEDFLAFAREQPLARAPVEAPTLLSVACELMAAEASARDVVVDIDASPAVLEADGSLLTAALVNLVKNAVQASP
PGGRVQVSGASKDGHYTIQVRDTGPGVPGTARERIFEPFFTTREKGTGLGLPLAHKIALAHGGALRLDSAPGDTRFILTL
PLGGKPGHGVSVTPSR

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=210, Percent_Identity=33.8095238095238, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=31.6666666666667, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788393, Length=339, Percent_Identity=28.6135693215339, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790861, Length=389, Percent_Identity=26.9922879177378, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790346, Length=279, Percent_Identity=29.7491039426523, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789808, Length=289, Percent_Identity=28.0276816608997, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787894, Length=298, Percent_Identity=29.1946308724832, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693157, Length=261, Percent_Identity=28.3524904214559, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790300, Length=220, Percent_Identity=27.7272727272727, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081816, Length=333, Percent_Identity=24.9249249249249, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=26.1603375527426, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082128, Length=285, Percent_Identity=25.2631578947368, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790551, Length=287, Percent_Identity=26.4808362369338, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786783, Length=305, Percent_Identity=25.9016393442623, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=215, Percent_Identity=26.9767441860465, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=25.4098360655738, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786600, Length=279, Percent_Identity=25.089605734767, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52598; Mature: 52467

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHVAPHTSRWRDALGSLSARLLVAFLVPTLLFVAVAGTAVYQLARVILEEELGTSLSGI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AAATATQVHGERLLTIEPGDDVASTRTWRNFVRTFENIAEASGVRRVYAVDTQGRMRVDV
HHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEE
GGGLPVGAEMPELARDRLELTRVFAGERAASQVLFTGSDGQRYKTGYAPILYEGRVVGAV
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCEEEEECCCCCEEECCCCCEEECCEEEEEE
GVEASAAFFGPLQRLSRTYAIMGAVALSALAVIALVTARGLARPLRRLMASALRIGRGDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TTPVPPEPTLEIGVLARELEDMRGALESRDRQLKMMLAGVAHEVRNPIGGIALFSGILKE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DLQAGAHADAGAHVERIQREVAYLQRIVEDFLAFAREQPLARAPVEAPTLLSVACELMAA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
EASARDVVVDIDASPAVLEADGSLLTAALVNLVKNAVQASPPGGRVQVSGASKDGHYTIQ
CCCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
VRDTGPGVPGTARERIFEPFFTTREKGTGLGLPLAHKIALAHGGALRLDSAPGDTRFILT
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCEEEEE
LPLGGKPGHGVSVTPSR
EECCCCCCCCEECCCCC
>Mature Secondary Structure 
PHVAPHTSRWRDALGSLSARLLVAFLVPTLLFVAVAGTAVYQLARVILEEELGTSLSGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AAATATQVHGERLLTIEPGDDVASTRTWRNFVRTFENIAEASGVRRVYAVDTQGRMRVDV
HHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEE
GGGLPVGAEMPELARDRLELTRVFAGERAASQVLFTGSDGQRYKTGYAPILYEGRVVGAV
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCEEEEECCCCCEEECCCCCEEECCEEEEEE
GVEASAAFFGPLQRLSRTYAIMGAVALSALAVIALVTARGLARPLRRLMASALRIGRGDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TTPVPPEPTLEIGVLARELEDMRGALESRDRQLKMMLAGVAHEVRNPIGGIALFSGILKE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DLQAGAHADAGAHVERIQREVAYLQRIVEDFLAFAREQPLARAPVEAPTLLSVACELMAA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
EASARDVVVDIDASPAVLEADGSLLTAALVNLVKNAVQASPPGGRVQVSGASKDGHYTIQ
CCCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
VRDTGPGVPGTARERIFEPFFTTREKGTGLGLPLAHKIALAHGGALRLDSAPGDTRFILT
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCEEEEE
LPLGGKPGHGVSVTPSR
EECCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]