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The map label for this gene is gatB

Identifier: 108759569

GI number: 108759569

Start: 1723924

End: 1725363

Strand: Direct

Name: gatB

Synonym: MXAN_1464

Alternate gene names: 108759569

Gene position: 1723924-1725363 (Clockwise)

Preceding gene: 108759562

Following gene: 108757164

Centisome position: 18.86

GC content: 66.6

Gene sequence:

>1440_bases
ATGCCCGTGAGCGATTTCCAGCCCGTCATCGGCCTCGAGGTCCACGCGCAGCTCCTCACGCAGTCCAAGATTTTCTGTGG
CTGCTCCACCGCGTTCGGCGCCGAGCCCAACCGCAACACCTGTCCGGTGTGCCTGGGCATGCCCGGTGTGCTGCCGGTGC
TCAACCAGCGCGTGGCGGAGTTCGCCGTGCGCACGGGGCTGGCGCTGGAGTGCACCATCCGCCCGACGAGCGTGTGGAGT
CGGAAGAACTACTTCTATCCGGACCTGCCCAAGGGCTATCAGATTACGCAGTTCGACCAGCCCATCTGCGAGCACGGGCG
GCTCGTCATCGACACGCCGCAGGGCGAGAAGGCCATCCGCATCCTCCGCATCCACATGGAAGAGGACGCGGGCAAGAGCG
TGCATGACGCCGGTGGGGGCCAGAGCCTGGTGGACCTCAACCGCGCGGGGGTGCCGCTGCTCGAAATCGTCAGCCAGCCG
GACCTGCGCGACGCGGACGAGGCGGTGGAGTACCTCAAGGCGATGCGGGACGTGCTCGTCTACCTGGGCGTCAACGACGG
CAACCTGGAGGAGGGGAGCTTCCGCTGCGATGCCAACGTGTCGGTGATGCCGAAGGGCTCCACCACGTTTGGCCAGCGCT
GCGAGCTGAAGAACCTCAACTCGTTCCGCTTCCTCAAGCAGGCCATCGAGTACGAGATTGCCCGGCAGGTGGACGTCATC
GAGTCGGGTGGCAAGGTGGTGCAGGAGACGCGCCTGTGGGACGTGAACAAGGGCGTCACCCGCTCCATGCGCAGCAAGGA
GGAGGCGCACGACTACCGGTACTTCCCGGAGCCGGACCTGCCGCCGCTGCACGTGAGCGCGGAGGCCATCGCCGCCGCGG
CGAAGGCGCTGCCAGAGCTGCCGCGCGCGAAGCTCCAGCGCTTCACCAGCCAGTACGGACTGCCCGCGTATGACGCCCGC
ATCCTCACCGCCGAGCGTCCGCTGGCCGACTACTTCGAGGCCTGCGCCGGGCACTACAAGGACTACAAGAAACTCTCCAA
CTGGTTCCTCGGAGAGCTGATGCGCCTGCTGAAGGAAGAGGGTACGCCGCTGTCCGCGCTGCGCTTCACGCCAGCACAGC
TGGGGGAGCTGCTGGGCGCTGTCGACCAGGGCATGGTGTCCGCCAACGCGGGCAAGGATGTGCTCGGGGAGATGTTCCGC
ACGGGCAAGGCGCCCGCGGACATCATCGCGGAGAAGGGCCTGGCCCAGGTGAGCGACACCGGCGCCATCGAGGCGGTGGT
GGACGACATCCTCGCGAAGAACGCGGGGGAGATTGAGAAGTACCGCGCCGGCAAGAAGCAGGTGTTCGGTTTCTTCGTGG
GCCAGGTGATGCGGGCCATGAAGGGCAAGGGCAACCCCGCCCTGGTCAACGAGCTGCTGAAGAAGAAGCTCGGCGACTAA

Upstream 100 bases:

>100_bases
GCCTGCTGCGCATTGCCCGCGCCTACGAGCGCGAGCACGACTTCTTCCGCCGCTCCGCGCCCCTGTAGGGCCCTCGAGCG
TCCGCCCTGGTGACACCGCC

Downstream 100 bases:

>100_bases
GGCGTGCCGTGTGCCGCGGTGGGGACGATGGCGTGCACCACGTCCTCCTCCTGCGTCGTCTGGGCCTGGGCGAGCACGCG
CACGTCGCCGGAGGCCGTGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MPVSDFQPVIGLEVHAQLLTQSKIFCGCSTAFGAEPNRNTCPVCLGMPGVLPVLNQRVAEFAVRTGLALECTIRPTSVWS
RKNYFYPDLPKGYQITQFDQPICEHGRLVIDTPQGEKAIRILRIHMEEDAGKSVHDAGGGQSLVDLNRAGVPLLEIVSQP
DLRDADEAVEYLKAMRDVLVYLGVNDGNLEEGSFRCDANVSVMPKGSTTFGQRCELKNLNSFRFLKQAIEYEIARQVDVI
ESGGKVVQETRLWDVNKGVTRSMRSKEEAHDYRYFPEPDLPPLHVSAEAIAAAAKALPELPRAKLQRFTSQYGLPAYDAR
ILTAERPLADYFEACAGHYKDYKKLSNWFLGELMRLLKEEGTPLSALRFTPAQLGELLGAVDQGMVSANAGKDVLGEMFR
TGKAPADIIAEKGLAQVSDTGAIEAVVDDILAKNAGEIEKYRAGKKQVFGFFVGQVMRAMKGKGNPALVNELLKKKLGD

Sequences:

>Translated_479_residues
MPVSDFQPVIGLEVHAQLLTQSKIFCGCSTAFGAEPNRNTCPVCLGMPGVLPVLNQRVAEFAVRTGLALECTIRPTSVWS
RKNYFYPDLPKGYQITQFDQPICEHGRLVIDTPQGEKAIRILRIHMEEDAGKSVHDAGGGQSLVDLNRAGVPLLEIVSQP
DLRDADEAVEYLKAMRDVLVYLGVNDGNLEEGSFRCDANVSVMPKGSTTFGQRCELKNLNSFRFLKQAIEYEIARQVDVI
ESGGKVVQETRLWDVNKGVTRSMRSKEEAHDYRYFPEPDLPPLHVSAEAIAAAAKALPELPRAKLQRFTSQYGLPAYDAR
ILTAERPLADYFEACAGHYKDYKKLSNWFLGELMRLLKEEGTPLSALRFTPAQLGELLGAVDQGMVSANAGKDVLGEMFR
TGKAPADIIAEKGLAQVSDTGAIEAVVDDILAKNAGEIEKYRAGKKQVFGFFVGQVMRAMKGKGNPALVNELLKKKLGD
>Mature_478_residues
PVSDFQPVIGLEVHAQLLTQSKIFCGCSTAFGAEPNRNTCPVCLGMPGVLPVLNQRVAEFAVRTGLALECTIRPTSVWSR
KNYFYPDLPKGYQITQFDQPICEHGRLVIDTPQGEKAIRILRIHMEEDAGKSVHDAGGGQSLVDLNRAGVPLLEIVSQPD
LRDADEAVEYLKAMRDVLVYLGVNDGNLEEGSFRCDANVSVMPKGSTTFGQRCELKNLNSFRFLKQAIEYEIARQVDVIE
SGGKVVQETRLWDVNKGVTRSMRSKEEAHDYRYFPEPDLPPLHVSAEAIAAAAKALPELPRAKLQRFTSQYGLPAYDARI
LTAERPLADYFEACAGHYKDYKKLSNWFLGELMRLLKEEGTPLSALRFTPAQLGELLGAVDQGMVSANAGKDVLGEMFRT
GKAPADIIAEKGLAQVSDTGAIEAVVDDILAKNAGEIEKYRAGKKQVFGFFVGQVMRAMKGKGNPALVNELLKKKLGD

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=492, Percent_Identity=38.6178861788618, Blast_Score=332, Evalue=6e-91,
Organism=Caenorhabditis elegans, GI115534094, Length=341, Percent_Identity=37.8299120234604, Blast_Score=227, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=500, Percent_Identity=30.2, Blast_Score=220, Evalue=4e-58,
Organism=Drosophila melanogaster, GI45550808, Length=495, Percent_Identity=35.1515151515151, Blast_Score=304, Evalue=7e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_MYXXD (Q1DCA2)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_629721.1
- ProteinModelPortal:   Q1DCA2
- SMR:   Q1DCA2
- STRING:   Q1DCA2
- GeneID:   4103573
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_1464
- TIGR:   MXAN_1464
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q1DCA2
- ProtClustDB:   PRK05477
- BioCyc:   MXAN246197:MXAN_1464-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 52680; Mature: 52549

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSDFQPVIGLEVHAQLLTQSKIFCGCSTAFGAEPNRNTCPVCLGMPGVLPVLNQRVAE
CCCCCCCCHHHHHHHHHHHHCCHHEEECCHHCCCCCCCCCCCEEECCCCHHHHHHHHHHH
FAVRTGLALECTIRPTSVWSRKNYFYPDLPKGYQITQFDQPICEHGRLVIDTPQGEKAIR
HHHHCCCEEEEEECCHHHHCCCCCCCCCCCCCCEECCCCCHHHHCCCEEEECCCCCCEEE
ILRIHMEEDAGKSVHDAGGGQSLVDLNRAGVPLLEIVSQPDLRDADEAVEYLKAMRDVLV
EEEEEECCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHEE
YLGVNDGNLEEGSFRCDANVSVMPKGSTTFGQRCELKNLNSFRFLKQAIEYEIARQVDVI
EECCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ESGGKVVQETRLWDVNKGVTRSMRSKEEAHDYRYFPEPDLPPLHVSAEAIAAAAKALPEL
HCCCHHHHHHHCEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PRAKLQRFTSQYGLPAYDARILTAERPLADYFEACAGHYKDYKKLSNWFLGELMRLLKEE
HHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTPLSALRFTPAQLGELLGAVDQGMVSANAGKDVLGEMFRTGKAPADIIAEKGLAQVSDT
CCCCHHHCCCHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHCCCCCHHHHHHCCHHHHCCC
GAIEAVVDDILAKNAGEIEKYRAGKKQVFGFFVGQVMRAMKGKGNPALVNELLKKKLGD
CHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVSDFQPVIGLEVHAQLLTQSKIFCGCSTAFGAEPNRNTCPVCLGMPGVLPVLNQRVAE
CCCCCCCHHHHHHHHHHHHCCHHEEECCHHCCCCCCCCCCCEEECCCCHHHHHHHHHHH
FAVRTGLALECTIRPTSVWSRKNYFYPDLPKGYQITQFDQPICEHGRLVIDTPQGEKAIR
HHHHCCCEEEEEECCHHHHCCCCCCCCCCCCCCEECCCCCHHHHCCCEEEECCCCCCEEE
ILRIHMEEDAGKSVHDAGGGQSLVDLNRAGVPLLEIVSQPDLRDADEAVEYLKAMRDVLV
EEEEEECCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHEE
YLGVNDGNLEEGSFRCDANVSVMPKGSTTFGQRCELKNLNSFRFLKQAIEYEIARQVDVI
EECCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ESGGKVVQETRLWDVNKGVTRSMRSKEEAHDYRYFPEPDLPPLHVSAEAIAAAAKALPEL
HCCCHHHHHHHCEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PRAKLQRFTSQYGLPAYDARILTAERPLADYFEACAGHYKDYKKLSNWFLGELMRLLKEE
HHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTPLSALRFTPAQLGELLGAVDQGMVSANAGKDVLGEMFRTGKAPADIIAEKGLAQVSDT
CCCCHHHCCCHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHCCCCCHHHHHHCCHHHHCCC
GAIEAVVDDILAKNAGEIEKYRAGKKQVFGFFVGQVMRAMKGKGNPALVNELLKKKLGD
CHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA