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The map label for this gene is nolF [H]

Identifier: 108759563

GI number: 108759563

Start: 3477499

End: 3478620

Strand: Reverse

Name: nolF [H]

Synonym: MXAN_2968

Alternate gene names: 108759563

Gene position: 3478620-3477499 (Counterclockwise)

Preceding gene: 108761343

Following gene: 108757162

Centisome position: 38.06

GC content: 71.57

Gene sequence:

>1122_bases
GTGACCGGCAGGAGCAGGACGACCGTGATTCGACGCATGTGGATGGCCGCGGCAGTGGCAGCGGTGGTGACGACGGGCTG
TGGCAAGGCCAGCAACAAGCCGGCCCTCCCCGAGCAGACGGGTCCCGCGGCATTGGGCGTGCGCGCCATCACTCCAGCCA
CGGAGTTGAGCGCCGACGTCACCCGCGTGACGGGGCAGATCCGCTCGAAGAACGAGGCGGTCCTGGGGCCCCAGGCGACC
GGCACCATCTCGAGGATGAACGTGCGGGTGGGCGACAAGGTGAAGAAGGGCCAGGTGCTGGCGGCGCTGGACGCCTCCAA
CGTCGCCATCAGCGTGGAGCAGGCGCGCGCGGTGAAGCAGGCCGCGGACGCGGCGCTGGAGCTGGCCACGTCCTCCCTGG
AGCGCACCCGGAAGGTGGCGGAGTCCGGTGGCGTGGCCGCCGCGGGGCTGGATCAGGCCGTGATTGGCCAGAAGCAGGCC
GCCGCCCAGGCCGCCCAGGCCGCCGCCGGGCTGCGGCTGGCGGAGGAGATGCTGCGTGACCACTCCATCATCGCCCCCTT
CGACGGCGTCATCACGGCCCGCACGAAGAACGTCGGTGACTCGGTGGCGATGACGCCCTCCACGCCCGTGTTCAGCATCG
TCGACACGACGGGGCTGGAAGTTCGCGCGCAGGTCCCCGAGTCCGTGGTGGACAAGGTCCGCGTGGGCAGCAAGACGCAG
GGCACGGTGAGCCCCAGCGGCGCGCGCTTCGACGTGCAGGTGGCCGTCGTGGGCGCGGTGGTGGACGCCTCCAACCGCAC
GGTGGAGGTGCTGGCGGACGTGGTCGGTGAGCCGTCCCCGGCGCTGCGTCCCGGCGCCCTGGTGGAGCTGGACTTCTCGA
CCGTGAATCAGGCCGACGACGACAAGGGCCTCTTCCTCCCCACCCAGGCCGTCAGCGCCCGGGGCCAGGAGGGCTTCGTG
TGGGTGGTGCAGGACGGCACGGTGCGCAAGCGGGATGTGCGCGTCGAGCGAGTGCTGCCTGGTTACGTCCGCATCCTCCA
GGGACTGGGCGCCGACGAGCGCGTGCTCGCCGACTCCTCCCTGGACGTGAAGGAGGGCACGGCCGTCCGCGTAGTGCAGT
GA

Upstream 100 bases:

>100_bases
AGTATCCCCTTGACAGACAGTTCAGAGACTGTAGTTTCCGGGCCGCAGACTTTCCATGTAGAAGCCGGCTCGCAAGTGTC
TCCCTGGGGCCAGGCGCCCC

Downstream 100 bases:

>100_bases
CGCCTGCCCTCCCCTCTCAAGTTCGGAGCTCCTGAATGCTCAAGACATTCATCACCCGCCCTGTGTTCACCGCGATGCTG
ATGCTCGCGGTGGTCGTTTT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTGRSRTTVIRRMWMAAAVAAVVTTGCGKASNKPALPEQTGPAALGVRAITPATELSADVTRVTGQIRSKNEAVLGPQAT
GTISRMNVRVGDKVKKGQVLAALDASNVAISVEQARAVKQAADAALELATSSLERTRKVAESGGVAAAGLDQAVIGQKQA
AAQAAQAAAGLRLAEEMLRDHSIIAPFDGVITARTKNVGDSVAMTPSTPVFSIVDTTGLEVRAQVPESVVDKVRVGSKTQ
GTVSPSGARFDVQVAVVGAVVDASNRTVEVLADVVGEPSPALRPGALVELDFSTVNQADDDKGLFLPTQAVSARGQEGFV
WVVQDGTVRKRDVRVERVLPGYVRILQGLGADERVLADSSLDVKEGTAVRVVQ

Sequences:

>Translated_373_residues
MTGRSRTTVIRRMWMAAAVAAVVTTGCGKASNKPALPEQTGPAALGVRAITPATELSADVTRVTGQIRSKNEAVLGPQAT
GTISRMNVRVGDKVKKGQVLAALDASNVAISVEQARAVKQAADAALELATSSLERTRKVAESGGVAAAGLDQAVIGQKQA
AAQAAQAAAGLRLAEEMLRDHSIIAPFDGVITARTKNVGDSVAMTPSTPVFSIVDTTGLEVRAQVPESVVDKVRVGSKTQ
GTVSPSGARFDVQVAVVGAVVDASNRTVEVLADVVGEPSPALRPGALVELDFSTVNQADDDKGLFLPTQAVSARGQEGFV
WVVQDGTVRKRDVRVERVLPGYVRILQGLGADERVLADSSLDVKEGTAVRVVQ
>Mature_372_residues
TGRSRTTVIRRMWMAAAVAAVVTTGCGKASNKPALPEQTGPAALGVRAITPATELSADVTRVTGQIRSKNEAVLGPQATG
TISRMNVRVGDKVKKGQVLAALDASNVAISVEQARAVKQAADAALELATSSLERTRKVAESGGVAAAGLDQAVIGQKQAA
AQAAQAAAGLRLAEEMLRDHSIIAPFDGVITARTKNVGDSVAMTPSTPVFSIVDTTGLEVRAQVPESVVDKVRVGSKTQG
TVSPSGARFDVQVAVVGAVVDASNRTVEVLADVVGEPSPALRPGALVELDFSTVNQADDDKGLFLPTQAVSARGQEGFVW
VVQDGTVRKRDVRVERVLPGYVRILQGLGADERVLADSSLDVKEGTAVRVVQ

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=319, Percent_Identity=27.8996865203762, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081951, Length=203, Percent_Identity=28.0788177339901, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38685; Mature: 38554

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRSRTTVIRRMWMAAAVAAVVTTGCGKASNKPALPEQTGPAALGVRAITPATELSADV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCEEEECCHHHHHHHH
TRVTGQIRSKNEAVLGPQATGTISRMNVRVGDKVKKGQVLAALDASNVAISVEQARAVKQ
HHHHHHHCCCCCEEECCCCCCCEEEEEEEECCCCCCCCEEEEECCCCEEEEHHHHHHHHH
AADAALELATSSLERTRKVAESGGVAAAGLDQAVIGQKQAAAQAAQAAAGLRLAEEMLRD
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
HSIIAPFDGVITARTKNVGDSVAMTPSTPVFSIVDTTGLEVRAQVPESVVDKVRVGSKTQ
CCEECCCCCEEEEEECCCCCCEEECCCCCEEEEEECCCCEEEECCCHHHHHHHHCCCCCC
GTVSPSGARFDVQVAVVGAVVDASNRTVEVLADVVGEPSPALRPGALVELDFSTVNQADD
CCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCCCCC
DKGLFLPTQAVSARGQEGFVWVVQDGTVRKRDVRVERVLPGYVRILQGLGADERVLADSS
CCCEEEEHHHHHCCCCCCEEEEEECCCEEHHHHHHHHHCHHHHHHHHHCCCCCEEECCCC
LDVKEGTAVRVVQ
CCCCCCCEEEEEC
>Mature Secondary Structure 
TGRSRTTVIRRMWMAAAVAAVVTTGCGKASNKPALPEQTGPAALGVRAITPATELSADV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCEEEECCHHHHHHHH
TRVTGQIRSKNEAVLGPQATGTISRMNVRVGDKVKKGQVLAALDASNVAISVEQARAVKQ
HHHHHHHCCCCCEEECCCCCCCEEEEEEEECCCCCCCCEEEEECCCCEEEEHHHHHHHHH
AADAALELATSSLERTRKVAESGGVAAAGLDQAVIGQKQAAAQAAQAAAGLRLAEEMLRD
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
HSIIAPFDGVITARTKNVGDSVAMTPSTPVFSIVDTTGLEVRAQVPESVVDKVRVGSKTQ
CCEECCCCCEEEEEECCCCCCEEECCCCCEEEEEECCCCEEEECCCHHHHHHHHCCCCCC
GTVSPSGARFDVQVAVVGAVVDASNRTVEVLADVVGEPSPALRPGALVELDFSTVNQADD
CCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCCCCC
DKGLFLPTQAVSARGQEGFVWVVQDGTVRKRDVRVERVLPGYVRILQGLGADERVLADSS
CCCEEEEHHHHHCCCCCCEEEEEECCCEEHHHHHHHHHCHHHHHHHHHCCCCCEEECCCC
LDVKEGTAVRVVQ
CCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]