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The map label for this gene is def [H]

Identifier: 108759554

GI number: 108759554

Start: 3947244

End: 3947849

Strand: Direct

Name: def [H]

Synonym: MXAN_3395

Alternate gene names: 108759554

Gene position: 3947244-3947849 (Clockwise)

Preceding gene: 108761296

Following gene: 108757572

Centisome position: 43.19

GC content: 70.46

Gene sequence:

>606_bases
ATGGTGCTCAAGATTGTCCAGGCAGGGGACCCGGTGCTGCGTCGGAAGGCGCGCGATTTGACGCCGGAGGAGATTGCCAG
CCCGGAGACGGCGCGGCTCATCGAGCAGATGCGCGACACGATGCGGGACGCACCCGGCGTCGGGCTGGCGGCGCCCCAGG
TGGGCGTGGGCCTGCGAGTCGTCGTCATCGAGGACCGGCCCGAGTATCAGGCCGGCCTCTCGGAGAGCGAGCGGGCGGCG
CGAGGACGGAAGCCCGTTCCCTTCCACGTGCTCATCAATCCCAGGCTGGTGGTGGAGGACGCGGCACCCGCGGAGTTCCA
CGAAGGGTGTCTGAGTGTGTCGGGCTTCGCGGCCCTGGTGCCCCGCGCCTGCGCCGTGCGCGTGGATGCACTCGATGAGC
ACGGCCAGCCCGTGACGGTCCAGGCCCGGGGCTGGTACGCGCGCATTCTCCAACACGAGCTGGACCACCTGGACGGCACG
CTCTACGTGGACCGGATGGAGACACGGAGCTTCACCACCGCGGAGAACCATCGCCGGTACCAGGCGGGGCGCAGCACCGA
GGAGCTGCGCGCCGAGCTGGGGCTGCCAGTGCCCGACAAGGGGTAG

Upstream 100 bases:

>100_bases
CCCGGGCTGGAACGGTGCGGAGTGGACACCACTCCCGGACGGCAGTGGGTGGAGCATTGTCGCCGGTCAGGAGCGTAGCG
GGCCCTGCTACGCTGGCGGC

Downstream 100 bases:

>100_bases
GGCACGACGTCTTTCGCGTCAGCCTTCGCGGCCGTGCGCCGCGGCCTTCAACTGGGCCGTGTCGACGCGGACGAAGATGG
AATCACCCACCGCGGTCAGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MVLKIVQAGDPVLRRKARDLTPEEIASPETARLIEQMRDTMRDAPGVGLAAPQVGVGLRVVVIEDRPEYQAGLSESERAA
RGRKPVPFHVLINPRLVVEDAAPAEFHEGCLSVSGFAALVPRACAVRVDALDEHGQPVTVQARGWYARILQHELDHLDGT
LYVDRMETRSFTTAENHRRYQAGRSTEELRAELGLPVPDKG

Sequences:

>Translated_201_residues
MVLKIVQAGDPVLRRKARDLTPEEIASPETARLIEQMRDTMRDAPGVGLAAPQVGVGLRVVVIEDRPEYQAGLSESERAA
RGRKPVPFHVLINPRLVVEDAAPAEFHEGCLSVSGFAALVPRACAVRVDALDEHGQPVTVQARGWYARILQHELDHLDGT
LYVDRMETRSFTTAENHRRYQAGRSTEELRAELGLPVPDKG
>Mature_201_residues
MVLKIVQAGDPVLRRKARDLTPEEIASPETARLIEQMRDTMRDAPGVGLAAPQVGVGLRVVVIEDRPEYQAGLSESERAA
RGRKPVPFHVLINPRLVVEDAAPAEFHEGCLSVSGFAALVPRACAVRVDALDEHGQPVTVQARGWYARILQHELDHLDGT
LYVDRMETRSFTTAENHRRYQAGRSTEELRAELGLPVPDKG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=169, Percent_Identity=43.1952662721894, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=36.969696969697, Blast_Score=94, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24645726, Length=167, Percent_Identity=40.7185628742515, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24645728, Length=169, Percent_Identity=40.2366863905325, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22188; Mature: 22188

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLKIVQAGDPVLRRKARDLTPEEIASPETARLIEQMRDTMRDAPGVGLAAPQVGVGLRV
CEEEEEECCCHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
VVIEDRPEYQAGLSESERAARGRKPVPFHVLINPRLVVEDAAPAEFHEGCLSVSGFAALV
EEECCCCCHHHCCCHHHHHHCCCCCCCEEEEECCEEEEECCCCHHHHHHHHCCCHHHHHH
PRACAVRVDALDEHGQPVTVQARGWYARILQHELDHLDGTLYVDRMETRSFTTAENHRRY
HHHHHEEEECHHCCCCEEEEEECHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHH
QAGRSTEELRAELGLPVPDKG
HCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVLKIVQAGDPVLRRKARDLTPEEIASPETARLIEQMRDTMRDAPGVGLAAPQVGVGLRV
CEEEEEECCCHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
VVIEDRPEYQAGLSESERAARGRKPVPFHVLINPRLVVEDAAPAEFHEGCLSVSGFAALV
EEECCCCCHHHCCCHHHHHHCCCCCCCEEEEECCEEEEECCCCHHHHHHHHCCCHHHHHH
PRACAVRVDALDEHGQPVTVQARGWYARILQHELDHLDGTLYVDRMETRSFTTAENHRRY
HHHHHEEEECHHCCCCEEEEEECHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHH
QAGRSTEELRAELGLPVPDKG
HCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]