Click here to switch to the map view.

The map label for this gene is rlmI [H]

Identifier: 108759477

GI number: 108759477

Start: 3279476

End: 3280633

Strand: Reverse

Name: rlmI [H]

Synonym: MXAN_2809

Alternate gene names: 108759477

Gene position: 3280633-3279476 (Counterclockwise)

Preceding gene: 108763918

Following gene: 108760544

Centisome position: 35.89

GC content: 70.9

Gene sequence:

>1158_bases
GTGAGAGGAGAACCCATCGTGCTCAGCACCTATCTGTCCCGCGACGCCGCCCGCCGACTCCGCCATGGCGCGCCATGGCT
GCGCCGGGAAGACATCGTCTCCATGGAAGGCACGCCGCAGCCCGGAGAGCCCGTACAGCTTCGGGACGAGGACGGCTCGG
TGCTGGGCCTGGGAGACGTGGATTTAGAGGCGTCCTACGCGGTGCGGCGGCTCGGCCAGCCGGACGAGGCGGTGGAGGGA
CTCATCCCACGCCATCTCCGCCACGCCTTCGAACGGCGGGCCCGGCTGGTGGACGACCCGCGCTTCTGCCGCGTCGTCAA
CGATGACGGGGACGGTCTGCCCGGCCTCATCGTGGACCGCTACGACACGCACTTCGTGGTGCAGACGCTCACCCGTGCCA
TGGACGCGCGCCATGAGGAACTGACGCGCGCGCTGGTGGAGGTGGCGGGCGCGGGCGCGGTGCTGCTGCGCAACGACACC
CTTCGCCGCAAAGCGCTGGGCCTGCCCACACAGCGCCCCCATGTCCTGTACGGCACCCCACCCCGTTGGTGCCGGCTGCT
GGAGATGGGGGCCCGCTTCACCGTGGACCTCACATACGGCCAGGGCACGGGCTACGCGTATGACCAGCGCGAGGTCCGCC
GCTTCGTGGCGCGCATGGCCCAGGGCTCGCGCGTGCTGGACGCCAGTTGCAACGTGGGAGGGCTCTTCGTCCACGCGGGG
GTGCACGGGGCCCGGCACATCCTCGCGTTCGACGCCGACGCGGACGCCGCGGACCTGGCGCGTGAGAACGCGGAGGCCAA
CGGCCTGCTGGGCCGGGTGATGGTGGAGACGGGCAAACCCCTCCAGGTCCTCCGTGCGGTGCGAGACACCTTCGACCTGG
TCCTGCTGGACACACCGGCCGTGGCGACCGGCGAGGAATTCGTGGAGTTGCTGCGTCACGCGCTTCGCAGGACGCGCCAC
GGTGGATGGTTGATGGTCACCGGGTACCATCCACCCCTCCCCCAGGACGGCTTTGACGACCTGGTGGCCACCGCCTGCGA
GGGCGAGGCCCGGATCGCCACCCGGCTGGCCCGCCCTGGCCTGCCGCCAGACCACCCGACCCTGGCCGGATCCCATGGGG
CCGAGCACCTCCACGCAATGGCGTTGGAAGTCAGCTGA

Upstream 100 bases:

>100_bases
CCCCCCGACGTGGCGCTCCGGGACGGGCAGCCATCCGCAGGCCAGCACCCCATGTTCTCCCCTGGCAGCACGCCCCGGGC
GCCGGTGCCCTCGGGTCGCG

Downstream 100 bases:

>100_bases
AGCCATCCACCCGCGCGGCCGATGGAGTGCTAGTGTCCGCCGCCGCAATGACCAACGAAAACGTGCCCGAGTCCGCGCCC
CCCGCCCCGGAAGGTTCCGT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MRGEPIVLSTYLSRDAARRLRHGAPWLRREDIVSMEGTPQPGEPVQLRDEDGSVLGLGDVDLEASYAVRRLGQPDEAVEG
LIPRHLRHAFERRARLVDDPRFCRVVNDDGDGLPGLIVDRYDTHFVVQTLTRAMDARHEELTRALVEVAGAGAVLLRNDT
LRRKALGLPTQRPHVLYGTPPRWCRLLEMGARFTVDLTYGQGTGYAYDQREVRRFVARMAQGSRVLDASCNVGGLFVHAG
VHGARHILAFDADADAADLARENAEANGLLGRVMVETGKPLQVLRAVRDTFDLVLLDTPAVATGEEFVELLRHALRRTRH
GGWLMVTGYHPPLPQDGFDDLVATACEGEARIATRLARPGLPPDHPTLAGSHGAEHLHAMALEVS

Sequences:

>Translated_385_residues
MRGEPIVLSTYLSRDAARRLRHGAPWLRREDIVSMEGTPQPGEPVQLRDEDGSVLGLGDVDLEASYAVRRLGQPDEAVEG
LIPRHLRHAFERRARLVDDPRFCRVVNDDGDGLPGLIVDRYDTHFVVQTLTRAMDARHEELTRALVEVAGAGAVLLRNDT
LRRKALGLPTQRPHVLYGTPPRWCRLLEMGARFTVDLTYGQGTGYAYDQREVRRFVARMAQGSRVLDASCNVGGLFVHAG
VHGARHILAFDADADAADLARENAEANGLLGRVMVETGKPLQVLRAVRDTFDLVLLDTPAVATGEEFVELLRHALRRTRH
GGWLMVTGYHPPLPQDGFDDLVATACEGEARIATRLARPGLPPDHPTLAGSHGAEHLHAMALEVS
>Mature_385_residues
MRGEPIVLSTYLSRDAARRLRHGAPWLRREDIVSMEGTPQPGEPVQLRDEDGSVLGLGDVDLEASYAVRRLGQPDEAVEG
LIPRHLRHAFERRARLVDDPRFCRVVNDDGDGLPGLIVDRYDTHFVVQTLTRAMDARHEELTRALVEVAGAGAVLLRNDT
LRRKALGLPTQRPHVLYGTPPRWCRLLEMGARFTVDLTYGQGTGYAYDQREVRRFVARMAQGSRVLDASCNVGGLFVHAG
VHGARHILAFDADADAADLARENAEANGLLGRVMVETGKPLQVLRAVRDTFDLVLLDTPAVATGEEFVELLRHALRRTRH
GGWLMVTGYHPPLPQDGFDDLVATACEGEARIATRLARPGLPPDHPTLAGSHGAEHLHAMALEVS

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=376, Percent_Identity=25.7978723404255, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787181, Length=229, Percent_Identity=27.0742358078603, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 42391; Mature: 42391

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGEPIVLSTYLSRDAARRLRHGAPWLRREDIVSMEGTPQPGEPVQLRDEDGSVLGLGDV
CCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEECCCC
DLEASYAVRRLGQPDEAVEGLIPRHLRHAFERRARLVDDPRFCRVVNDDGDGLPGLIVDR
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECC
YDTHFVVQTLTRAMDARHEELTRALVEVAGAGAVLLRNDTLRRKALGLPTQRPHVLYGTP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCCCEEEECCC
PRWCRLLEMGARFTVDLTYGQGTGYAYDQREVRRFVARMAQGSRVLDASCNVGGLFVHAG
HHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCEEEEECC
VHGARHILAFDADADAADLARENAEANGLLGRVMVETGKPLQVLRAVRDTFDLVLLDTPA
CCCCEEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEEECCCC
VATGEEFVELLRHALRRTRHGGWLMVTGYHPPLPQDGFDDLVATACEGEARIATRLARPG
CCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCC
LPPDHPTLAGSHGAEHLHAMALEVS
CCCCCCCCCCCCCHHHHHHEEEECC
>Mature Secondary Structure
MRGEPIVLSTYLSRDAARRLRHGAPWLRREDIVSMEGTPQPGEPVQLRDEDGSVLGLGDV
CCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEECCCC
DLEASYAVRRLGQPDEAVEGLIPRHLRHAFERRARLVDDPRFCRVVNDDGDGLPGLIVDR
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECC
YDTHFVVQTLTRAMDARHEELTRALVEVAGAGAVLLRNDTLRRKALGLPTQRPHVLYGTP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCCCEEEECCC
PRWCRLLEMGARFTVDLTYGQGTGYAYDQREVRRFVARMAQGSRVLDASCNVGGLFVHAG
HHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCEEEEECC
VHGARHILAFDADADAADLARENAEANGLLGRVMVETGKPLQVLRAVRDTFDLVLLDTPA
CCCCEEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEEECCCC
VATGEEFVELLRHALRRTRHGGWLMVTGYHPPLPQDGFDDLVATACEGEARIATRLARPG
CCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCC
LPPDHPTLAGSHGAEHLHAMALEVS
CCCCCCCCCCCCCHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA