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The map label for this gene is rluB [H]

Identifier: 108759403

GI number: 108759403

Start: 4616462

End: 4618699

Strand: Reverse

Name: rluB [H]

Synonym: MXAN_3839

Alternate gene names: 108759403

Gene position: 4618699-4616462 (Counterclockwise)

Preceding gene: 108760008

Following gene: 108763908

Centisome position: 50.53

GC content: 73.24

Gene sequence:

>2238_bases
ATGGCGGCCGAACGACTACAGAAGTACCTGGCCCGCGCGGGAGTTGCTTCGCGCCGGCATGCAGAAGAGCTGATTACCGC
GGGCCGCGTCGCGGTGAACAACAAGACGGTGACGGAGCTGGGCAGCCGGGTGGAGCCCGGCACCGACCTGGTGACGGTGG
ACGGGACGCTGGTCACGCCTCCGGATGAATCCTCCTACTTCCTGCTCTACAAGCCCGTGGGCGTCGTGACGACGCTGTCG
GATCCGCAGGGCCGGCCCACGGTGGCCAACTACGTGGAAGAGACGGGCAAGCGCCTGTTCCCGGTCGGCCGTCTGGACTA
CGACGCCGAGGGCGCGCTGCTGTTCACGGATGATGGGGCGCTGGCGCACAAGCTGACGCACCCCAGCTTCCAGGTGCCGC
GCACCTACCTGGCGAAGGTGAAGGGCGCTCCGGACACGGCCACGCTGGACAAGTTGCGCGGCGGCGTGCGGCTCGAAGAC
GGCATGGCCACGCCGGTGTCGGTGAGCGTATTCGAGGCGGCGGAGCGGAACACCTGGCTGAAGATTGTCGTGGCGGAAGG
ACGTCCGCACCTCATTAAGCGGCTGTGCGCGGCGGTGGGGCACCCGGTGGTGCGTCTGTTCCGTCCGTCCTACGCCGGCG
TGGGCGTGGAAGGCCTGCGCCCCGGCGAGCTGCGCCCGCTCAAGACGAGCGAGGTGGAGCTGCTGAACGCGGTGTCGGAT
GGCAAGGCCGCGCCGCCGACGGGGGAGCTGAGGCTGCCTCCGCGCCGGCATGGCCGGGCGGCTCCGGGCTTCGACTCTGA
CGATGAGGCGGAGCTGTCCATGGATGATGATGCGCCCGTGGCCGCCAAGCCCGCGCGCAAGGCGCCGGCCCGTGCGGCCA
AAACCGCGACCGTGGGTGGTTCGAGCCTGGCGCGTTTCGGTCGGCCCAAGGGGGCTTCGGCCGAGGGCAAGCCCGCGCGC
CGTTCGTTCGGTGGCGATGAGGGCGCGCCGCGTGGCCGTTCCTTCGGCGGTGACAAGGCTGGCGCTCCGCGTGGCCGCTC
CTTCGGTGGTGACGAGGGCGCGCCGCGTGGCCGCTCCTTCGGCGGTGACAAGGCTGGCGCTCCGCGTGGCCGTTCCTTCG
GTGGTGACGAGGGCAGGCCCGCGCGCCGTTCCTTCGGCAGTGACGAGGGCAAGCCCGCGCGCCGCTCCTTCGGCAGTGAC
GAGGGCAGGCCCGCGCGTCGTTCCTTCGGCGGTGACGAGGGCAAGCCCGCGCGCCGCTCCTTCGGCAGTGACGAGGGCAG
GCCCGCGCGTCGTTCCTTCGGCGGTGACAAGGCAGGCTCGCCGCGTGGCCGTTCCTTCGGCAGTGACGAGGGCAGGCCCG
CGCGCCGTTCCTTCGGCGGTGACGAGGGTAAGCCCGCGCGCCGCTCCTTCGGTGGTGACGAGGGCAGGCCTGCACGCCGC
TCCTTCGGTGGTGACGAGGGAAGGCCCGCACGCCGTTCGTTCGGTGGCGATGATGGCGCACCGCGCGGCCGTTCCTTCGG
CGGTGACAAGGCAGGTGCTCCTCGCAGCCGTGGTGCGGGCCGGTCCTTCGGCGGTGACGAGGGCGGACCCGCGCGCCGCT
CCTTCGGCGGTGATGCGGGCAGCGGTCCGCGTAGCCGCGACACGGGCGCACGCGGTGCGGGTCGCGCATTCGGTGGTGCT
GGCGGCGCCCGTCGCTCGTTTGGCGGTGACGAGGGCGGAGCCCCCAAGAGCCGTGGCGCGGGTCGTCCGGAGCGCCGTGA
GTGGAGCGCGGGCGGAGACGCCGCGCCGCGGACTCGCGCACCGCGCGCGGGTGGCGAAGGCCGTCCGGAGCGCAAGTCGT
GGGGTGCTGGCGCCGGCGCCAGCCGTGCCCCGCGGGGTGAAGGCCGTCCGTACTCGCCCCGTTCCGGAGGAGCGGGTGCT
GGCCGCCCGGAGCGCCGTGAGTGGAAGCCTCGCGCCGAAGGGGCAGACGCTCCGCGTGGCCGGGGTGGTCCTCGCCGTGA
GGGCTCCGAGGGCGGACGCCCCGCACGCAGCACCTGGACGCCCACCGACGAAAGCGGGAATCCCCGTGAACGCGTCGTCC
GGGCCGGGGCTCGCAAGGGCCCTCCCGCCGAGAAGTCCGGCGGATTCCAGGACTGGGGCAAGAAGAAGGAGCGGGCGAGC
GCCCCGAGCTGGAGCAATGAGCGTCCCCGAGGCGGCGCGGGCCGGCCTCCTCCCCGGGGGCCTCGCCGTCCGCGTTGA

Upstream 100 bases:

>100_bases
GGTCAGCTCCAGCGTCCTGGACACGACGCCCCCGGAACCCGAGACGGGTGATACGACGGGGCCCAAGCCCGAGTGACGGG
CAGCATGGAAGAAGGATGGT

Downstream 100 bases:

>100_bases
TACATGACGCGGTGGATTGGGCGGGAATCGAGGGGGATTGATATAACGGGCGCCAACTCCTACACCGGAGACCGATGCGA
ACCGGCGCGCGCCCCCTCAT

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 745; Mature: 744

Protein sequence:

>745_residues
MAAERLQKYLARAGVASRRHAEELITAGRVAVNNKTVTELGSRVEPGTDLVTVDGTLVTPPDESSYFLLYKPVGVVTTLS
DPQGRPTVANYVEETGKRLFPVGRLDYDAEGALLFTDDGALAHKLTHPSFQVPRTYLAKVKGAPDTATLDKLRGGVRLED
GMATPVSVSVFEAAERNTWLKIVVAEGRPHLIKRLCAAVGHPVVRLFRPSYAGVGVEGLRPGELRPLKTSEVELLNAVSD
GKAAPPTGELRLPPRRHGRAAPGFDSDDEAELSMDDDAPVAAKPARKAPARAAKTATVGGSSLARFGRPKGASAEGKPAR
RSFGGDEGAPRGRSFGGDKAGAPRGRSFGGDEGAPRGRSFGGDKAGAPRGRSFGGDEGRPARRSFGSDEGKPARRSFGSD
EGRPARRSFGGDEGKPARRSFGSDEGRPARRSFGGDKAGSPRGRSFGSDEGRPARRSFGGDEGKPARRSFGGDEGRPARR
SFGGDEGRPARRSFGGDDGAPRGRSFGGDKAGAPRSRGAGRSFGGDEGGPARRSFGGDAGSGPRSRDTGARGAGRAFGGA
GGARRSFGGDEGGAPKSRGAGRPERREWSAGGDAAPRTRAPRAGGEGRPERKSWGAGAGASRAPRGEGRPYSPRSGGAGA
GRPERREWKPRAEGADAPRGRGGPRREGSEGGRPARSTWTPTDESGNPRERVVRAGARKGPPAEKSGGFQDWGKKKERAS
APSWSNERPRGGAGRPPPRGPRRPR

Sequences:

>Translated_745_residues
MAAERLQKYLARAGVASRRHAEELITAGRVAVNNKTVTELGSRVEPGTDLVTVDGTLVTPPDESSYFLLYKPVGVVTTLS
DPQGRPTVANYVEETGKRLFPVGRLDYDAEGALLFTDDGALAHKLTHPSFQVPRTYLAKVKGAPDTATLDKLRGGVRLED
GMATPVSVSVFEAAERNTWLKIVVAEGRPHLIKRLCAAVGHPVVRLFRPSYAGVGVEGLRPGELRPLKTSEVELLNAVSD
GKAAPPTGELRLPPRRHGRAAPGFDSDDEAELSMDDDAPVAAKPARKAPARAAKTATVGGSSLARFGRPKGASAEGKPAR
RSFGGDEGAPRGRSFGGDKAGAPRGRSFGGDEGAPRGRSFGGDKAGAPRGRSFGGDEGRPARRSFGSDEGKPARRSFGSD
EGRPARRSFGGDEGKPARRSFGSDEGRPARRSFGGDKAGSPRGRSFGSDEGRPARRSFGGDEGKPARRSFGGDEGRPARR
SFGGDEGRPARRSFGGDDGAPRGRSFGGDKAGAPRSRGAGRSFGGDEGGPARRSFGGDAGSGPRSRDTGARGAGRAFGGA
GGARRSFGGDEGGAPKSRGAGRPERREWSAGGDAAPRTRAPRAGGEGRPERKSWGAGAGASRAPRGEGRPYSPRSGGAGA
GRPERREWKPRAEGADAPRGRGGPRREGSEGGRPARSTWTPTDESGNPRERVVRAGARKGPPAEKSGGFQDWGKKKERAS
APSWSNERPRGGAGRPPPRGPRRPR
>Mature_744_residues
AAERLQKYLARAGVASRRHAEELITAGRVAVNNKTVTELGSRVEPGTDLVTVDGTLVTPPDESSYFLLYKPVGVVTTLSD
PQGRPTVANYVEETGKRLFPVGRLDYDAEGALLFTDDGALAHKLTHPSFQVPRTYLAKVKGAPDTATLDKLRGGVRLEDG
MATPVSVSVFEAAERNTWLKIVVAEGRPHLIKRLCAAVGHPVVRLFRPSYAGVGVEGLRPGELRPLKTSEVELLNAVSDG
KAAPPTGELRLPPRRHGRAAPGFDSDDEAELSMDDDAPVAAKPARKAPARAAKTATVGGSSLARFGRPKGASAEGKPARR
SFGGDEGAPRGRSFGGDKAGAPRGRSFGGDEGAPRGRSFGGDKAGAPRGRSFGGDEGRPARRSFGSDEGKPARRSFGSDE
GRPARRSFGGDEGKPARRSFGSDEGRPARRSFGGDKAGSPRGRSFGSDEGRPARRSFGGDEGKPARRSFGGDEGRPARRS
FGGDEGRPARRSFGGDDGAPRGRSFGGDKAGAPRSRGAGRSFGGDEGGPARRSFGGDAGSGPRSRDTGARGAGRAFGGAG
GARRSFGGDEGGAPKSRGAGRPERREWSAGGDAAPRTRAPRAGGEGRPERKSWGAGAGASRAPRGEGRPYSPRSGGAGAG
RPERREWKPRAEGADAPRGRGGPRREGSEGGRPARSTWTPTDESGNPRERVVRAGARKGPPAEKSGGFQDWGKKKERASA
PSWSNERPRGGAGRPPPRGPRRPR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=221, Percent_Identity=42.9864253393665, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=35.042735042735, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=31.404958677686, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081838, Length=174, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 77867; Mature: 77736

Theoretical pI: Translated: 11.63; Mature: 11.63

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAERLQKYLARAGVASRRHAEELITAGRVAVNNKTVTELGSRVEPGTDLVTVDGTLVTP
CCHHHHHHHHHHHCHHHHHHHHHHHHHCCEEECCCHHHHHHCCCCCCCCEEEECCEEECC
PDESSYFLLYKPVGVVTTLSDPQGRPTVANYVEETGKRLFPVGRLDYDAEGALLFTDDGA
CCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCEEEECCCH
LAHKLTHPSFQVPRTYLAKVKGAPDTATLDKLRGGVRLEDGMATPVSVSVFEAAERNTWL
HHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHCCCCEECCCCCCCCEEHHHHHHCCCCEE
KIVVAEGRPHLIKRLCAAVGHPVVRLFRPSYAGVGVEGLRPGELRPLKTSEVELLNAVSD
EEEEECCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
GKAAPPTGELRLPPRRHGRAAPGFDSDDEAELSMDDDAPVAAKPARKAPARAAKTATVGG
CCCCCCCCCEECCHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHCCHHHHHHHCCCC
SSLARFGRPKGASAEGKPARRSFGGDEGAPRGRSFGGDKAGAPRGRSFGGDEGAPRGRSF
HHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGDKAGAPRGRSFGGDEGRPARRSFGSDEGKPARRSFGSDEGRPARRSFGGDEGKPARRS
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCHHHHCCCCCCCHHHHH
FGSDEGRPARRSFGGDKAGSPRGRSFGSDEGRPARRSFGGDEGKPARRSFGGDEGRPARR
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCCCCCHHH
SFGGDEGRPARRSFGGDDGAPRGRSFGGDKAGAPRSRGAGRSFGGDEGGPARRSFGGDAG
HCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
SGPRSRDTGARGAGRAFGGAGGARRSFGGDEGGAPKSRGAGRPERREWSAGGDAAPRTRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
PRAGGEGRPERKSWGAGAGASRAPRGEGRPYSPRSGGAGAGRPERREWKPRAEGADAPRG
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
RGGPRREGSEGGRPARSTWTPTDESGNPRERVVRAGARKGPPAEKSGGFQDWGKKKERAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
APSWSNERPRGGAGRPPPRGPRRPR
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAERLQKYLARAGVASRRHAEELITAGRVAVNNKTVTELGSRVEPGTDLVTVDGTLVTP
CHHHHHHHHHHHCHHHHHHHHHHHHHCCEEECCCHHHHHHCCCCCCCCEEEECCEEECC
PDESSYFLLYKPVGVVTTLSDPQGRPTVANYVEETGKRLFPVGRLDYDAEGALLFTDDGA
CCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCEEEECCCH
LAHKLTHPSFQVPRTYLAKVKGAPDTATLDKLRGGVRLEDGMATPVSVSVFEAAERNTWL
HHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHCCCCEECCCCCCCCEEHHHHHHCCCCEE
KIVVAEGRPHLIKRLCAAVGHPVVRLFRPSYAGVGVEGLRPGELRPLKTSEVELLNAVSD
EEEEECCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
GKAAPPTGELRLPPRRHGRAAPGFDSDDEAELSMDDDAPVAAKPARKAPARAAKTATVGG
CCCCCCCCCEECCHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHCCHHHHHHHCCCC
SSLARFGRPKGASAEGKPARRSFGGDEGAPRGRSFGGDKAGAPRGRSFGGDEGAPRGRSF
HHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGDKAGAPRGRSFGGDEGRPARRSFGSDEGKPARRSFGSDEGRPARRSFGGDEGKPARRS
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCHHHHCCCCCCCHHHHH
FGSDEGRPARRSFGGDKAGSPRGRSFGSDEGRPARRSFGGDEGKPARRSFGGDEGRPARR
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCCCCCHHH
SFGGDEGRPARRSFGGDDGAPRGRSFGGDKAGAPRSRGAGRSFGGDEGGPARRSFGGDAG
HCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
SGPRSRDTGARGAGRAFGGAGGARRSFGGDEGGAPKSRGAGRPERREWSAGGDAAPRTRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
PRAGGEGRPERKSWGAGAGASRAPRGEGRPYSPRSGGAGAGRPERREWKPRAEGADAPRG
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
RGGPRREGSEGGRPARSTWTPTDESGNPRERVVRAGARKGPPAEKSGGFQDWGKKKERAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
APSWSNERPRGGAGRPPPRGPRRPR
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]