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The map label for this gene is wcaJ [H]

Identifier: 108759368

GI number: 108759368

Start: 3779636

End: 3781003

Strand: Direct

Name: wcaJ [H]

Synonym: MXAN_3229

Alternate gene names: 108759368

Gene position: 3779636-3781003 (Clockwise)

Preceding gene: 108758270

Following gene: 108762647

Centisome position: 41.35

GC content: 66.74

Gene sequence:

>1368_bases
GTGCTCCGCGTTTTTCACCATTATTTTTCTGCAAAGAAACTGACGTTCTTCCTCGCCGAGAGTTCGGCAATCGCGTTGGC
CTGCGTGATGGGCGCGGCGGCCTGCGCGGCACTCTTCGCGCCCGAAGGGACGCACACGCCGCTGTCGCAACTGTGGCCCA
CGCTGCTGTGGATGGGCGCGGCGTTCGTCATCACCTTCCAGTTCACGCTGTACCTGTTGGACTTGTATGACCTCCGTGTG
GCCGCGGAGGACCGGGTGAGGGGATACCGGTTCCTCAAGGCCGCGGGTGTCACGGCGATGGTGGCGGGCGGCGTCATGCT
CGTCACCCCGCTGGTGGTGCCGATGCAGCTTCCACCGGGCACGCTGCTGGGCGGGGCCATGGGCGCCCTGGCCGGGACGT
TGATGGTGCGGGTGTCCATCCGCGCCCTGGTGGGAGCGCCGCACTCGGTGCTCATCGTCGGAGAAGGGCCCAAGGCGCGC
GCGGTGGCCAGCGCGATTGACGCGGGCGGGGAGGGCACCTACCGCATCGTGGGCCTGACGGACCCCCGTACCTCGGGTGA
GGCGCTGGAGGAGACGGCGGCCCGGCTGAACGCGGCCTATGTGGTGCAGGCCGCGGATGACATGCGCGGGGCCAACTGGG
TGGACGGCCTGCTGCGCTGCCGGCTCCAGGGCCGGCGGGTGTATGAGGCCACGGGCTTCTGTGAGCGGGTGCTGCGCCGG
ATTCCGGTGCAGTTCCTGCGGGCGAGCGACTTCGCCTTCGCGGACGAGCTCACCGTGTCCCCGCTGCGCCGCACGCTGAA
GCGGGGCTTCGACATCGCGGTGGCATCCCTCCTGCTAATGCTGTCCGCGCCCTTCCTGTTGCTGGTCGTGGTGGCCATCA
AGCTGGATTCGAAGGGCCCCATCTTCTACCGGCAGGAAAGGACCGGGCTGTTCGGGAGCACGTATCACCTGTGGAAGTTC
CGCAGCATGCGCACGGACGCGGAGAAGCATGGAGCGGTGTGGGCGAAGGCGAACGATGACCGTGTCACGCGGGTGGGCCG
CTTCATCCGCCGTGCGCGCATCGACGAGATTCCCCAGGTCTTCAACATCCTGACGGGAGACATGAGTTTCGTGGGGCCCC
GCCCCGAGAGGCCAGTGTTCGTCGAGCAGTTGAAGGAGCAGATTCCGTTCTACGGCCTGCGGGAGGCCGTCAAGCCAGGC
CTGACGGGGTGGGCTCAGATTCGCTACCCCTACGGCGCGTCGGTGGAGGATGCGCGGAACAAGCTGGAGTTCGACCTGTA
CTACGTGAAGAACGGTTCGCTGTTCCTGGATGTGGGAATCATCTTCCACACCGTGAGGCATGTGCTTCTCGGTCGTGGGG
CGCGGTAG

Upstream 100 bases:

>100_bases
GAAGTAGCGGTGGGTGGCTTTGGTTCCGGTTCCCGCCTCCTGGTGACAGGGGGCGGGGTCGGAGATGGAAGAGGGTCGGG
CGGGCAGGTTGGAGGGGCAC

Downstream 100 bases:

>100_bases
GGCATTTCGCTTTCCCGGCCTCCGCTGAGGGAGGCCGGGAGGTGGGCCCGCCTGGGGAGGCGGGCTGGGGCGTCGTGGAT
GGGGGTAAGCGTCATGGTGG

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MLRVFHHYFSAKKLTFFLAESSAIALACVMGAAACAALFAPEGTHTPLSQLWPTLLWMGAAFVITFQFTLYLLDLYDLRV
AAEDRVRGYRFLKAAGVTAMVAGGVMLVTPLVVPMQLPPGTLLGGAMGALAGTLMVRVSIRALVGAPHSVLIVGEGPKAR
AVASAIDAGGEGTYRIVGLTDPRTSGEALEETAARLNAAYVVQAADDMRGANWVDGLLRCRLQGRRVYEATGFCERVLRR
IPVQFLRASDFAFADELTVSPLRRTLKRGFDIAVASLLLMLSAPFLLLVVVAIKLDSKGPIFYRQERTGLFGSTYHLWKF
RSMRTDAEKHGAVWAKANDDRVTRVGRFIRRARIDEIPQVFNILTGDMSFVGPRPERPVFVEQLKEQIPFYGLREAVKPG
LTGWAQIRYPYGASVEDARNKLEFDLYYVKNGSLFLDVGIIFHTVRHVLLGRGAR

Sequences:

>Translated_455_residues
MLRVFHHYFSAKKLTFFLAESSAIALACVMGAAACAALFAPEGTHTPLSQLWPTLLWMGAAFVITFQFTLYLLDLYDLRV
AAEDRVRGYRFLKAAGVTAMVAGGVMLVTPLVVPMQLPPGTLLGGAMGALAGTLMVRVSIRALVGAPHSVLIVGEGPKAR
AVASAIDAGGEGTYRIVGLTDPRTSGEALEETAARLNAAYVVQAADDMRGANWVDGLLRCRLQGRRVYEATGFCERVLRR
IPVQFLRASDFAFADELTVSPLRRTLKRGFDIAVASLLLMLSAPFLLLVVVAIKLDSKGPIFYRQERTGLFGSTYHLWKF
RSMRTDAEKHGAVWAKANDDRVTRVGRFIRRARIDEIPQVFNILTGDMSFVGPRPERPVFVEQLKEQIPFYGLREAVKPG
LTGWAQIRYPYGASVEDARNKLEFDLYYVKNGSLFLDVGIIFHTVRHVLLGRGAR
>Mature_455_residues
MLRVFHHYFSAKKLTFFLAESSAIALACVMGAAACAALFAPEGTHTPLSQLWPTLLWMGAAFVITFQFTLYLLDLYDLRV
AAEDRVRGYRFLKAAGVTAMVAGGVMLVTPLVVPMQLPPGTLLGGAMGALAGTLMVRVSIRALVGAPHSVLIVGEGPKAR
AVASAIDAGGEGTYRIVGLTDPRTSGEALEETAARLNAAYVVQAADDMRGANWVDGLLRCRLQGRRVYEATGFCERVLRR
IPVQFLRASDFAFADELTVSPLRRTLKRGFDIAVASLLLMLSAPFLLLVVVAIKLDSKGPIFYRQERTGLFGSTYHLWKF
RSMRTDAEKHGAVWAKANDDRVTRVGRFIRRARIDEIPQVFNILTGDMSFVGPRPERPVFVEQLKEQIPFYGLREAVKPG
LTGWAQIRYPYGASVEDARNKLEFDLYYVKNGSLFLDVGIIFHTVRHVLLGRGAR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=198, Percent_Identity=43.4343434343434, Blast_Score=171, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 50156; Mature: 50156

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVFHHYFSAKKLTFFLAESSAIALACVMGAAACAALFAPEGTHTPLSQLWPTLLWMGA
CCCHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AFVITFQFTLYLLDLYDLRVAAEDRVRGYRFLKAAGVTAMVAGGVMLVTPLVVPMQLPPG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCHHEECHHHEEEECCCC
TLLGGAMGALAGTLMVRVSIRALVGAPHSVLIVGEGPKARAVASAIDAGGEGTYRIVGLT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEC
DPRTSGEALEETAARLNAAYVVQAADDMRGANWVDGLLRCRLQGRRVYEATGFCERVLRR
CCCCCHHHHHHHHHHCCCEEEEEECHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
IPVQFLRASDFAFADELTVSPLRRTLKRGFDIAVASLLLMLSAPFLLLVVVAIKLDSKGP
CCHHHHHCCCCCHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
IFYRQERTGLFGSTYHLWKFRSMRTDAEKHGAVWAKANDDRVTRVGRFIRRARIDEIPQV
EEEEECCCCCCCCCCHHHHHHHHCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
FNILTGDMSFVGPRPERPVFVEQLKEQIPFYGLREAVKPGLTGWAQIRYPYGASVEDARN
HHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCHHHHHC
KLEFDLYYVKNGSLFLDVGIIFHTVRHVLLGRGAR
CEEEEEEEEECCCEEEEHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLRVFHHYFSAKKLTFFLAESSAIALACVMGAAACAALFAPEGTHTPLSQLWPTLLWMGA
CCCHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AFVITFQFTLYLLDLYDLRVAAEDRVRGYRFLKAAGVTAMVAGGVMLVTPLVVPMQLPPG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCHHEECHHHEEEECCCC
TLLGGAMGALAGTLMVRVSIRALVGAPHSVLIVGEGPKARAVASAIDAGGEGTYRIVGLT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEC
DPRTSGEALEETAARLNAAYVVQAADDMRGANWVDGLLRCRLQGRRVYEATGFCERVLRR
CCCCCHHHHHHHHHHCCCEEEEEECHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
IPVQFLRASDFAFADELTVSPLRRTLKRGFDIAVASLLLMLSAPFLLLVVVAIKLDSKGP
CCHHHHHCCCCCHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
IFYRQERTGLFGSTYHLWKFRSMRTDAEKHGAVWAKANDDRVTRVGRFIRRARIDEIPQV
EEEEECCCCCCCCCCHHHHHHHHCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
FNILTGDMSFVGPRPERPVFVEQLKEQIPFYGLREAVKPGLTGWAQIRYPYGASVEDARN
HHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCHHHHHC
KLEFDLYYVKNGSLFLDVGIIFHTVRHVLLGRGAR
CEEEEEEEEECCCEEEEHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]