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The map label for this gene is glnA [H]

Identifier: 108759227

GI number: 108759227

Start: 1041586

End: 1043025

Strand: Reverse

Name: glnA [H]

Synonym: MXAN_0912

Alternate gene names: 108759227

Gene position: 1043025-1041586 (Counterclockwise)

Preceding gene: 108763856

Following gene: 108762452

Centisome position: 11.41

GC content: 64.38

Gene sequence:

>1440_bases
ATGGACGCGAAGGGGCTACGGACGTTCCTGGAGATTCCCTACGACGAGCTCGAGGAGCGCAACCTCAAGGTCAAGGAGCA
GCGCCTCAAGCACACCGCGTCGGAGAAGCTCCGTGACGAGCGCATCGAGTACCTGACGAAGGAACGCGCCATCAAGGCGG
TCACCGTGTGCTTCACCGACCTCGAGGGTCGGCTGCACATGCTGGACTACGACAAGAAGTTCCTCATCAAGAGCGCCGAC
AACCTCACCTTCGACGGCTCCTCCATCCGGGGCTTCTCCGCGCAGCAGGAGAGTGACCTGCGGCTGAACATCGACTGGAG
CGCCTTCTATTGGCTGCCCTCGGACATCTTCGGTCCGGGCAAGGTGCTGGTGTTCAGCGAGGTGCACGAGCGTGACGGCA
CGCAGTACCACTCCGACCTGCGTGGCAGGCTGAAGCTGCTCACCGAGTCGATGTACCAGAAAGACGGCACGGTGGTTCAC
GCGGCGCCTGAAATCGAGGGCTTCCTCTTCAAGGGCCGCGACGCCGAGCACCACTTCCACGAGAAGGGCCACTTCGACTT
CATCTCCACCGGCGGCTACTACCACTCGCTGCCCGGTGACGTGCTGCGCACGTTCATCGACAAGGTGGCCGAGGCCCAGC
GCGCCATGGGCTTCCAGAACGAGAAGGACCACCCCGAAGTGGCGCCCAGCCAGTTCGAGATCAACTTCTCGTACAGCGAA
GCCCTCATCGCGGCCGACCAGATTCAGCTCTACAAGCTGCTCAGCCGCCAGGTGGCCGCGCAGTTGGACTGCACCGCCAG
CTTCCTCCCGAAGCCGGTGACGGGCGTCAATGGCAACGGCATGCACATGAACCTGTCGCTGTCCAAGGGCGGGAAGAACC
TCTTCCACGACCAGAACGGCCCGGACGGCCTCAGCCCCATGGGCTGGGACTTCATCGACCGCCTCCTCACCAGCGCGAAT
GACATCTGCCTCACGCTCAACTCCAGCGTGAACGCGTACCGCCGCCTGGACCCGCACTTCGAGGCCCCCAACCAGATCAA
GGCCAGCGCCAACAACCGCGGTGCCATGGTGCGCATCCCCTTCGGAAACGAGCGCAGCGCGCGCATCGAGTGCCGCTCGG
TGGCGCCGGACGCCAACCCCTACCTGGTGCTCTACACCCTGCTGCGCACCGGCCTGGAGGGCCCGCAGCCGCAGGAGGAC
GCGGAGGCCAAGCGCAGCCGCACCCGCTTCCTGCCGGACAACATCTTCGACGCCATCCGCATCTTCAAGGGCAGCCAGTT
CGTGGCGTCCATCCTGGGTGAGGGCGTGCAGAGCAAGTTCGCCGAGCTCAAGACGACCACGGCCGAGCGCAGCCCGAAGC
AGCTGGGCACCCGTGTGAAGTACTCGGAGGTCAAGTTCCACCACGAGGTCACCAACCAGTTCCTCTGGAACCAGTTCTAG

Upstream 100 bases:

>100_bases
GATTTGGGCTTGATTTCCGCTCGGCAAATGTAAATACCTGGGCCCACGGACTCATCCCGCCGCACCGACGGCGAAGAATC
CGGCCTGGGAGGCACGAACA

Downstream 100 bases:

>100_bases
GAACGGACGTCCGCACCGCCGGACTTCAGGAGCCGCCGCCCTCCCGCGACATGGGGAGGCGGCGGCTTCGTCCGTTACAG
CGGCTCGCCGCGAAGCGCCG

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MDAKGLRTFLEIPYDELEERNLKVKEQRLKHTASEKLRDERIEYLTKERAIKAVTVCFTDLEGRLHMLDYDKKFLIKSAD
NLTFDGSSIRGFSAQQESDLRLNIDWSAFYWLPSDIFGPGKVLVFSEVHERDGTQYHSDLRGRLKLLTESMYQKDGTVVH
AAPEIEGFLFKGRDAEHHFHEKGHFDFISTGGYYHSLPGDVLRTFIDKVAEAQRAMGFQNEKDHPEVAPSQFEINFSYSE
ALIAADQIQLYKLLSRQVAAQLDCTASFLPKPVTGVNGNGMHMNLSLSKGGKNLFHDQNGPDGLSPMGWDFIDRLLTSAN
DICLTLNSSVNAYRRLDPHFEAPNQIKASANNRGAMVRIPFGNERSARIECRSVAPDANPYLVLYTLLRTGLEGPQPQED
AEAKRSRTRFLPDNIFDAIRIFKGSQFVASILGEGVQSKFAELKTTTAERSPKQLGTRVKYSEVKFHHEVTNQFLWNQF

Sequences:

>Translated_479_residues
MDAKGLRTFLEIPYDELEERNLKVKEQRLKHTASEKLRDERIEYLTKERAIKAVTVCFTDLEGRLHMLDYDKKFLIKSAD
NLTFDGSSIRGFSAQQESDLRLNIDWSAFYWLPSDIFGPGKVLVFSEVHERDGTQYHSDLRGRLKLLTESMYQKDGTVVH
AAPEIEGFLFKGRDAEHHFHEKGHFDFISTGGYYHSLPGDVLRTFIDKVAEAQRAMGFQNEKDHPEVAPSQFEINFSYSE
ALIAADQIQLYKLLSRQVAAQLDCTASFLPKPVTGVNGNGMHMNLSLSKGGKNLFHDQNGPDGLSPMGWDFIDRLLTSAN
DICLTLNSSVNAYRRLDPHFEAPNQIKASANNRGAMVRIPFGNERSARIECRSVAPDANPYLVLYTLLRTGLEGPQPQED
AEAKRSRTRFLPDNIFDAIRIFKGSQFVASILGEGVQSKFAELKTTTAERSPKQLGTRVKYSEVKFHHEVTNQFLWNQF
>Mature_479_residues
MDAKGLRTFLEIPYDELEERNLKVKEQRLKHTASEKLRDERIEYLTKERAIKAVTVCFTDLEGRLHMLDYDKKFLIKSAD
NLTFDGSSIRGFSAQQESDLRLNIDWSAFYWLPSDIFGPGKVLVFSEVHERDGTQYHSDLRGRLKLLTESMYQKDGTVVH
AAPEIEGFLFKGRDAEHHFHEKGHFDFISTGGYYHSLPGDVLRTFIDKVAEAQRAMGFQNEKDHPEVAPSQFEINFSYSE
ALIAADQIQLYKLLSRQVAAQLDCTASFLPKPVTGVNGNGMHMNLSLSKGGKNLFHDQNGPDGLSPMGWDFIDRLLTSAN
DICLTLNSSVNAYRRLDPHFEAPNQIKASANNRGAMVRIPFGNERSARIECRSVAPDANPYLVLYTLLRTGLEGPQPQED
AEAKRSRTRFLPDNIFDAIRIFKGSQFVASILGEGVQSKFAELKTTTAERSPKQLGTRVKYSEVKFHHEVTNQFLWNQF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=387, Percent_Identity=31.5245478036176, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081870, Length=323, Percent_Identity=30.3405572755418, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 54379; Mature: 54379

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAKGLRTFLEIPYDELEERNLKVKEQRLKHTASEKLRDERIEYLTKERAIKAVTVCFTD
CCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEC
LEGRLHMLDYDKKFLIKSADNLTFDGSSIRGFSAQQESDLRLNIDWSAFYWLPSDIFGPG
CCCEEEEECCCCCEEEECCCCCEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCC
KVLVFSEVHERDGTQYHSDLRGRLKLLTESMYQKDGTVVHAAPEIEGFLFKGRDAEHHFH
CEEEEECHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHC
EKGHFDFISTGGYYHSLPGDVLRTFIDKVAEAQRAMGFQNEKDHPEVAPSQFEINFSYSE
CCCCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHH
ALIAADQIQLYKLLSRQVAAQLDCTASFLPKPVTGVNGNGMHMNLSLSKGGKNLFHDQNG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
PDGLSPMGWDFIDRLLTSANDICLTLNSSVNAYRRLDPHFEAPNQIKASANNRGAMVRIP
CCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCCCCEECCCCCCCCEEEEE
FGNERSARIECRSVAPDANPYLVLYTLLRTGLEGPQPQEDAEAKRSRTRFLPDNIFDAIR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
IFKGSQFVASILGEGVQSKFAELKTTTAERSPKQLGTRVKYSEVKFHHEVTNQFLWNQF
HHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDAKGLRTFLEIPYDELEERNLKVKEQRLKHTASEKLRDERIEYLTKERAIKAVTVCFTD
CCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEC
LEGRLHMLDYDKKFLIKSADNLTFDGSSIRGFSAQQESDLRLNIDWSAFYWLPSDIFGPG
CCCEEEEECCCCCEEEECCCCCEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCC
KVLVFSEVHERDGTQYHSDLRGRLKLLTESMYQKDGTVVHAAPEIEGFLFKGRDAEHHFH
CEEEEECHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHC
EKGHFDFISTGGYYHSLPGDVLRTFIDKVAEAQRAMGFQNEKDHPEVAPSQFEINFSYSE
CCCCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHH
ALIAADQIQLYKLLSRQVAAQLDCTASFLPKPVTGVNGNGMHMNLSLSKGGKNLFHDQNG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
PDGLSPMGWDFIDRLLTSANDICLTLNSSVNAYRRLDPHFEAPNQIKASANNRGAMVRIP
CCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCCCCEECCCCCCCCEEEEE
FGNERSARIECRSVAPDANPYLVLYTLLRTGLEGPQPQEDAEAKRSRTRFLPDNIFDAIR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
IFKGSQFVASILGEGVQSKFAELKTTTAERSPKQLGTRVKYSEVKFHHEVTNQFLWNQF
HHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2572584 [H]