Click here to switch to the map view.

The map label for this gene is greB [H]

Identifier: 108758975

GI number: 108758975

Start: 3732642

End: 3733172

Strand: Direct

Name: greB [H]

Synonym: MXAN_3186

Alternate gene names: 108758975

Gene position: 3732642-3733172 (Clockwise)

Preceding gene: 108761007

Following gene: 108760216

Centisome position: 40.84

GC content: 69.49

Gene sequence:

>531_bases
ATGTCCCAGGACGTACATCCGGATGAACAGGAGACCGAGGACGACGCCGAGCGGGCCCCGTTCCGCCGCTACCTCACCCG
GGCGGGCGCCGAGCGCCTGCACCGCGAGCTCGTCCACCTTCTCAACGAGGAGCGCCCCAAGGTGACGGCCGAGGTCTCCG
CTGCCGCCGCTCAGGGCGACCGCTCCGAGAACGCCGAGTACATCTACGGGAAGAAGCGCCTGCGCGAAATCGACCGCCGC
CTCCGTTTCCTCCAGAAGCGCCTGGACACCGCCACCATCGTCACGCCCGCCGAGCAGACCGACCGCTCCCGCGTCTTCTT
CGGGGCGACGGTGAGCCTGGAGGACGAGGACGGCGGCCGCAGCACCTACCAGATCGTGGGTTCCGACGAGATTGACGCCT
CGGGCGGGCGCATCAGCGTCGAATCCCCCATGGGGCGGGCGCTCCTTCGCAAGGCCGTGGGAGACTCGGTCGAGGTGCGG
CGGCCGCGCGGGGACATCGAACTCACGGTGGTGGACATCCGCTACGAATAG

Upstream 100 bases:

>100_bases
AGCGCATGGGGCTGTTTCTGACACGCCCCGACCGCTTTCCGGTGAACGTCAGGGCCAGCGCCCTTCGCCGCGTGTCACCC
CAGGGCGATATACAGGAACC

Downstream 100 bases:

>100_bases
GTGGAAGCCATGCCTCGGATTCAGCCCATCAACCCCCGCTACCGTCGCATCTATCAGGCGCTGAGCGGGCCGGACCTGGC
CGTTTCCGAGAGCGCGGGGA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MSQDVHPDEQETEDDAERAPFRRYLTRAGAERLHRELVHLLNEERPKVTAEVSAAAAQGDRSENAEYIYGKKRLREIDRR
LRFLQKRLDTATIVTPAEQTDRSRVFFGATVSLEDEDGGRSTYQIVGSDEIDASGGRISVESPMGRALLRKAVGDSVEVR
RPRGDIELTVVDIRYE

Sequences:

>Translated_176_residues
MSQDVHPDEQETEDDAERAPFRRYLTRAGAERLHRELVHLLNEERPKVTAEVSAAAAQGDRSENAEYIYGKKRLREIDRR
LRFLQKRLDTATIVTPAEQTDRSRVFFGATVSLEDEDGGRSTYQIVGSDEIDASGGRISVESPMGRALLRKAVGDSVEVR
RPRGDIELTVVDIRYE
>Mature_175_residues
SQDVHPDEQETEDDAERAPFRRYLTRAGAERLHRELVHLLNEERPKVTAEVSAAAAQGDRSENAEYIYGKKRLREIDRRL
RFLQKRLDTATIVTPAEQTDRSRVFFGATVSLEDEDGGRSTYQIVGSDEIDASGGRISVESPMGRALLRKAVGDSVEVRR
PRGDIELTVVDIRYE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=151, Percent_Identity=50.9933774834437, Blast_Score=142, Evalue=9e-36,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=34.8684210526316, Blast_Score=93, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 19929; Mature: 19798

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDVHPDEQETEDDAERAPFRRYLTRAGAERLHRELVHLLNEERPKVTAEVSAAAAQGD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCC
RSENAEYIYGKKRLREIDRRLRFLQKRLDTATIVTPAEQTDRSRVFFGATVSLEDEDGGR
CCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEEEEECCCCCC
STYQIVGSDEIDASGGRISVESPMGRALLRKAVGDSVEVRRPRGDIELTVVDIRYE
EEEEEECCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCEEEEEEEEEEC
>Mature Secondary Structure 
SQDVHPDEQETEDDAERAPFRRYLTRAGAERLHRELVHLLNEERPKVTAEVSAAAAQGD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCC
RSENAEYIYGKKRLREIDRRLRFLQKRLDTATIVTPAEQTDRSRVFFGATVSLEDEDGGR
CCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEEEEECCCCCC
STYQIVGSDEIDASGGRISVESPMGRALLRKAVGDSVEVRRPRGDIELTVVDIRYE
EEEEEECCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA