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The map label for this gene is nth [H]

Identifier: 108758880

GI number: 108758880

Start: 3688394

End: 3689026

Strand: Reverse

Name: nth [H]

Synonym: MXAN_3148

Alternate gene names: 108758880

Gene position: 3689026-3688394 (Counterclockwise)

Preceding gene: 108763780

Following gene: 108759218

Centisome position: 40.36

GC content: 65.09

Gene sequence:

>633_bases
ATGAATCCCGCCGAGAAAGCCGCCCTCTTCCTCGAGCGCCTCCGGGAAGCCCATCCGGATGCGCGCTACGAACTCAACTG
GTCCACGCCTTTCGAGCTGCTCGTCGCCACCATCCTGGCCGCGCAGTGCACCGACGAGCGCGTCAACCGCGTCACCGCCA
CGGTCTTCCCCAAGTACCCGGGGCCACAGGCCTTCGCCGACGCGGACACCGCCGCGCTGGAGGAGGACCTCAAGCCCACC
GGCTTCTTCAAGCAGAAGACGAAGTCCGTGCAGGCCATGAGCCGCGCGCTCCTCGACAAGTTCGGCGGTGAGGTCCCCCA
CACCATCGACGAACTGGTGACGCTCCCGGGCGTGGCGCGGAAGACGGCCAACGTCGTCCTCAACACCGCCTTCAATCTCC
CCTCCGGCATCATCGTCGACACCCACGTGGCGCGCGTCAGCCAGCGCCTGGGCCTGACGAAGAAAGACAAACCCGAGGCG
ATTGAAGAAGACCTGATGAAGCTGGTGCCCCAGGAGCAGTGGACCTTCTTCGGCCCCGCCACCGTCCTCCATGGGCGTTA
CACCTGCACCGCAAAGAAGCCGAAATGCGACGACTGCATCGTGAAGGATGTGTGCCCGCGTATCGGCGTGTAA

Upstream 100 bases:

>100_bases
GTATTCGGCTTTTTCGCGACCAAGCGCCTGCTGGGCGGCGCCCAAACGCTCGTGGCGCATGTCAGACGCTCCGAGTAGGG
TGGTGCTCAGTCACGCCACC

Downstream 100 bases:

>100_bases
CCAAATCCCTGTCCGCCTGTGGGATATGAATGTCTTTTCGCGTACGCTCCTGGGACGCGAAAAGACGCGCATCAAAAGCG
GAGGAATTCGCAATCGAGGT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNPAEKAALFLERLREAHPDARYELNWSTPFELLVATILAAQCTDERVNRVTATVFPKYPGPQAFADADTAALEEDLKPT
GFFKQKTKSVQAMSRALLDKFGGEVPHTIDELVTLPGVARKTANVVLNTAFNLPSGIIVDTHVARVSQRLGLTKKDKPEA
IEEDLMKLVPQEQWTFFGPATVLHGRYTCTAKKPKCDDCIVKDVCPRIGV

Sequences:

>Translated_210_residues
MNPAEKAALFLERLREAHPDARYELNWSTPFELLVATILAAQCTDERVNRVTATVFPKYPGPQAFADADTAALEEDLKPT
GFFKQKTKSVQAMSRALLDKFGGEVPHTIDELVTLPGVARKTANVVLNTAFNLPSGIIVDTHVARVSQRLGLTKKDKPEA
IEEDLMKLVPQEQWTFFGPATVLHGRYTCTAKKPKCDDCIVKDVCPRIGV
>Mature_210_residues
MNPAEKAALFLERLREAHPDARYELNWSTPFELLVATILAAQCTDERVNRVTATVFPKYPGPQAFADADTAALEEDLKPT
GFFKQKTKSVQAMSRALLDKFGGEVPHTIDELVTLPGVARKTANVVLNTAFNLPSGIIVDTHVARVSQRLGLTKKDKPEA
IEEDLMKLVPQEQWTFFGPATVLHGRYTCTAKKPKCDDCIVKDVCPRIGV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=182, Percent_Identity=32.967032967033, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=43.9024390243902, Blast_Score=170, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=35.5932203389831, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=25.1336898395722, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=26.4367816091954, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=34.2696629213483, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23221; Mature: 23221

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPAEKAALFLERLREAHPDARYELNWSTPFELLVATILAAQCTDERVNRVTATVFPKYP
CCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
GPQAFADADTAALEEDLKPTGFFKQKTKSVQAMSRALLDKFGGEVPHTIDELVTLPGVAR
CCCHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHH
KTANVVLNTAFNLPSGIIVDTHVARVSQRLGLTKKDKPEAIEEDLMKLVPQEQWTFFGPA
HHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHCCEEECCH
TVLHGRYTCTAKKPKCDDCIVKDVCPRIGV
HHHCCCEEECCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNPAEKAALFLERLREAHPDARYELNWSTPFELLVATILAAQCTDERVNRVTATVFPKYP
CCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
GPQAFADADTAALEEDLKPTGFFKQKTKSVQAMSRALLDKFGGEVPHTIDELVTLPGVAR
CCCHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHH
KTANVVLNTAFNLPSGIIVDTHVARVSQRLGLTKKDKPEAIEEDLMKLVPQEQWTFFGPA
HHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHCCEEECCH
TVLHGRYTCTAKKPKCDDCIVKDVCPRIGV
HHHCCCEEECCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]