Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 108758688

GI number: 108758688

Start: 3887698

End: 3890103

Strand: Direct

Name: slt [H]

Synonym: MXAN_3344

Alternate gene names: 108758688

Gene position: 3887698-3890103 (Clockwise)

Preceding gene: 108761379

Following gene: 108759696

Centisome position: 42.54

GC content: 70.95

Gene sequence:

>2406_bases
ATGAAGCCCTTCCTTTCCAAGCTCGCCGTCGCCGCCTCCGCGCTCCTGATGACCGCGCAGGCACCCGCCCCGAAGGCGGT
TTCGTCCACCCCGGCCGAGCCCGAGGCGTCCGAAGCGCCGGCCCAGCACCCCAGCAACGCGCCTTCAGAGGCGCAGTTGT
CGCCCCCTCCCGACTCGGAGAAGGCGCCGCTTCCGGTGGGCTACGTGGAGGTGCTCAACCCCGCCTTCCCCAATGCCGCG
CCGCCCACACCGGTGGTGCACCGGGGCCGCCGCTACGCGCTGGAGGACCTGGCGCCTTACTTCGGCGAGGGCAAGAAGAA
GGAGGCCCGTGACGCGTTCGACCGTGGACAGTACACGCGCGCCCGCGAGCTGCTGAAGGACGAGGGCGACAGCCCGCCGG
TGCGCTACCTGCGCGCCCTGGCCGCCGTCCGCGCCGGCGATGACGCGTCCGCCGCCAAGGAATTCGCCGCGCTGGTCCCG
GACTATCCCGCGCTGAAGGACCGCTGCCTGACGCATGGCGGCGTGGCGCTGGAGAGCCTGCGCCGCTTCGATGAGGCCGC
GGTGCTGCTGGAGCAGGTGCCACCCGAGTCCAAGCTGTACGTGGACGCGCGGCTGGCCCTGTCGCGCGTGCTGCGCAAGA
AGAAGGACGCGGCGGGCGCCATGGCCGCGCTGGAGCCGCTCACGTCGCGCGCGGCGCCCAGCTGGGGCCGCAACGTGGGC
GCCGAGGCGCTGATGGCCATCGCCGACATCGCCGCGGAGAAGAAGGACAAGGCCGCCGAGCGCGCCGCGCTGTGGCGCCT
GTGGGCCGCGCACCCGCTGTCCGCCTTGTCGAAGCAGGCCGAGAAGCGCCTCAAGGGGCAGACGCCGCCCGTCGATGCGA
GGGTGGGGCGTGGCGAGGCGCTGGTGGAGCTGCACCGGAACAAGGCCGGCCTGGAGCAGTTGGAGCCGCTGCTGCCGAAG
CTGGCGCTTCCGGACCCGCTGGCCTGCCGCGCGCACTTCGCCTTCGGCAAGGGCATGCGCAAGGAGCGCCAGCACACGCG
CGCCATCCAGGTGCTGACGCCCGTGGCGGAGAAGTGCCAGGACCGCGACCTGCTGGCGCGCGTGCTGTACGTGCTCGGCT
CGTCGCGCTCCATCGTCGACCAGGCGCGGGGCACGGAGACCTACGAGCGGCTGGCGCGCGAGTTCCCGGACCACTCGTTC
GCGGATGACGGCCTCTTCTACGCGGCCGACCTCTACCTGAAGACGGGCCGTCCGAAGGAGGCCATGGCCCGCCTGGACAC
GCTGGCCCGGCTGTACCCGCAGGGGGACTTCCTGGGCGAGGCGCTCTTCAAGGCGTTCTGGATTGCTCGCACCACGGGCG
TGGAGGACTCCGGCCTGTCGTTCCTGGACCGCATCGAGGCGCAGTTCGCCAAGGCGGACGAGAGCTACGACGTGGAGCGC
GCGCGCTACTGGCGGGCGCGGACGATGCAAGAGAAGGGCAACATCCAGGGCGCGGCGGAGCTGTTCGAGAAGCTCTCCGT
GGACCACCCGGCGACCTACTACGGGCTGATGGCGCGCTCGCAGCTGGCGAAGGTGGACCCGCCACGGCTGGAGAAGGTGT
CCGCCGAAATCTTCGCCGTGCCCGAGGCCGCCAGCCCCTGGCCGCTGTTCGCCGGCCCCATGGGCGAGGACCCGCACTTC
CGCGCGGGCGTGGAGCTGTACCGCCTGGGCTTCAAGGAAGCCGTGTCGTCCGAGATGCTGGCCGTCAACCGGACCAATCT
GCCCGCGGAGTCCGTGCGCCTGCTGGTGCTGGTGCTGCACGAGGCCGGGGACGAGCGCTCCGCCCACGCGGTGGCGCGGC
TCGCCCTGCGCAAGGACTTGAGCGGGCGCATCACTCCGGAGACGCGCGTGGTGTGGGAGGTGGCCTATCCCAACGCCTTC
CGCGACAGCATCGAGAAGCACACCGCGGACGCGGGCGTGGAGCCGGACCTGCTCCAGGCGTTGATGCGGGAGGAGAGCGC
GCTGGACCCCAAGGCGCTCTCGTGGGCGGGAGCGATGGGCCTCACCCAGTTGATGCCGTCCACCGCGAAGGGCGTGGCGC
GCGAGCTGAAGCTCAAGCGCTTCAGCGTGGACCAGTTGCTCCAGCCCGACCTCAACATCCGCATGGGGGCCCACTATCTT
GGCGGCTTGCTCAAGCGCTTCAACGGGCACACGCCCTATGCGGTGGGCAGCTACAACGCCGGTCCCGGCGCGGTGAATCG
TTGGAGGTCCGACAGGCCGGACCTGGCGCTGGACGCATGGGTGGAGGAAATCCCCATCTCCGAGACACGCGGTTACATCA
AGCGCGTGCTGCGCTCCTTCAACACGTACCAACTGCTGTACGGACGGGCGCCCAAGCTGCCGGTGCTCAAGAGCGCCGCG
AAGTAG

Upstream 100 bases:

>100_bases
CGGACAGTCTAACCTGTGTCAGCCTCCGGAAATTTTCCATGACCTGCCGGTTGTCCTGGGGCGAGCCCGCGCGCATCTCC
CTGGGACCCAGCCCCCGCGT

Downstream 100 bases:

>100_bases
GTGCGCTGCAGCCATCCGGGCGCCTGCTGGGTGGTGTCGGTGGAGAATTCGTGCGGAATGGGGCGCGGACGCCGCGGGTT
ATGTCCACGCCCGGAAAAAG

Product: transglycosylase SLT domain-containing protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKPFLSKLAVAASALLMTAQAPAPKAVSSTPAEPEASEAPAQHPSNAPSEAQLSPPPDSEKAPLPVGYVEVLNPAFPNAA
PPTPVVHRGRRYALEDLAPYFGEGKKKEARDAFDRGQYTRARELLKDEGDSPPVRYLRALAAVRAGDDASAAKEFAALVP
DYPALKDRCLTHGGVALESLRRFDEAAVLLEQVPPESKLYVDARLALSRVLRKKKDAAGAMAALEPLTSRAAPSWGRNVG
AEALMAIADIAAEKKDKAAERAALWRLWAAHPLSALSKQAEKRLKGQTPPVDARVGRGEALVELHRNKAGLEQLEPLLPK
LALPDPLACRAHFAFGKGMRKERQHTRAIQVLTPVAEKCQDRDLLARVLYVLGSSRSIVDQARGTETYERLAREFPDHSF
ADDGLFYAADLYLKTGRPKEAMARLDTLARLYPQGDFLGEALFKAFWIARTTGVEDSGLSFLDRIEAQFAKADESYDVER
ARYWRARTMQEKGNIQGAAELFEKLSVDHPATYYGLMARSQLAKVDPPRLEKVSAEIFAVPEAASPWPLFAGPMGEDPHF
RAGVELYRLGFKEAVSSEMLAVNRTNLPAESVRLLVLVLHEAGDERSAHAVARLALRKDLSGRITPETRVVWEVAYPNAF
RDSIEKHTADAGVEPDLLQALMREESALDPKALSWAGAMGLTQLMPSTAKGVARELKLKRFSVDQLLQPDLNIRMGAHYL
GGLLKRFNGHTPYAVGSYNAGPGAVNRWRSDRPDLALDAWVEEIPISETRGYIKRVLRSFNTYQLLYGRAPKLPVLKSAA
K

Sequences:

>Translated_801_residues
MKPFLSKLAVAASALLMTAQAPAPKAVSSTPAEPEASEAPAQHPSNAPSEAQLSPPPDSEKAPLPVGYVEVLNPAFPNAA
PPTPVVHRGRRYALEDLAPYFGEGKKKEARDAFDRGQYTRARELLKDEGDSPPVRYLRALAAVRAGDDASAAKEFAALVP
DYPALKDRCLTHGGVALESLRRFDEAAVLLEQVPPESKLYVDARLALSRVLRKKKDAAGAMAALEPLTSRAAPSWGRNVG
AEALMAIADIAAEKKDKAAERAALWRLWAAHPLSALSKQAEKRLKGQTPPVDARVGRGEALVELHRNKAGLEQLEPLLPK
LALPDPLACRAHFAFGKGMRKERQHTRAIQVLTPVAEKCQDRDLLARVLYVLGSSRSIVDQARGTETYERLAREFPDHSF
ADDGLFYAADLYLKTGRPKEAMARLDTLARLYPQGDFLGEALFKAFWIARTTGVEDSGLSFLDRIEAQFAKADESYDVER
ARYWRARTMQEKGNIQGAAELFEKLSVDHPATYYGLMARSQLAKVDPPRLEKVSAEIFAVPEAASPWPLFAGPMGEDPHF
RAGVELYRLGFKEAVSSEMLAVNRTNLPAESVRLLVLVLHEAGDERSAHAVARLALRKDLSGRITPETRVVWEVAYPNAF
RDSIEKHTADAGVEPDLLQALMREESALDPKALSWAGAMGLTQLMPSTAKGVARELKLKRFSVDQLLQPDLNIRMGAHYL
GGLLKRFNGHTPYAVGSYNAGPGAVNRWRSDRPDLALDAWVEEIPISETRGYIKRVLRSFNTYQLLYGRAPKLPVLKSAA
K
>Mature_801_residues
MKPFLSKLAVAASALLMTAQAPAPKAVSSTPAEPEASEAPAQHPSNAPSEAQLSPPPDSEKAPLPVGYVEVLNPAFPNAA
PPTPVVHRGRRYALEDLAPYFGEGKKKEARDAFDRGQYTRARELLKDEGDSPPVRYLRALAAVRAGDDASAAKEFAALVP
DYPALKDRCLTHGGVALESLRRFDEAAVLLEQVPPESKLYVDARLALSRVLRKKKDAAGAMAALEPLTSRAAPSWGRNVG
AEALMAIADIAAEKKDKAAERAALWRLWAAHPLSALSKQAEKRLKGQTPPVDARVGRGEALVELHRNKAGLEQLEPLLPK
LALPDPLACRAHFAFGKGMRKERQHTRAIQVLTPVAEKCQDRDLLARVLYVLGSSRSIVDQARGTETYERLAREFPDHSF
ADDGLFYAADLYLKTGRPKEAMARLDTLARLYPQGDFLGEALFKAFWIARTTGVEDSGLSFLDRIEAQFAKADESYDVER
ARYWRARTMQEKGNIQGAAELFEKLSVDHPATYYGLMARSQLAKVDPPRLEKVSAEIFAVPEAASPWPLFAGPMGEDPHF
RAGVELYRLGFKEAVSSEMLAVNRTNLPAESVRLLVLVLHEAGDERSAHAVARLALRKDLSGRITPETRVVWEVAYPNAF
RDSIEKHTADAGVEPDLLQALMREESALDPKALSWAGAMGLTQLMPSTAKGVARELKLKRFSVDQLLQPDLNIRMGAHYL
GGLLKRFNGHTPYAVGSYNAGPGAVNRWRSDRPDLALDAWVEEIPISETRGYIKRVLRSFNTYQLLYGRAPKLPVLKSAA
K

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=324, Percent_Identity=26.8518518518519, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 87958; Mature: 87958

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFLSKLAVAASALLMTAQAPAPKAVSSTPAEPEASEAPAQHPSNAPSEAQLSPPPDSE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KAPLPVGYVEVLNPAFPNAAPPTPVVHRGRRYALEDLAPYFGEGKKKEARDAFDRGQYTR
CCCCCCHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHH
ARELLKDEGDSPPVRYLRALAAVRAGDDASAAKEFAALVPDYPALKDRCLTHGGVALESL
HHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
RRFDEAAVLLEQVPPESKLYVDARLALSRVLRKKKDAAGAMAALEPLTSRAAPSWGRNVG
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
AEALMAIADIAAEKKDKAAERAALWRLWAAHPLSALSKQAEKRLKGQTPPVDARVGRGEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVELHRNKAGLEQLEPLLPKLALPDPLACRAHFAFGKGMRKERQHTRAIQVLTPVAEKCQ
HHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
DRDLLARVLYVLGSSRSIVDQARGTETYERLAREFPDHSFADDGLFYAADLYLKTGRPKE
CHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHH
AMARLDTLARLYPQGDFLGEALFKAFWIARTTGVEDSGLSFLDRIEAQFAKADESYDVER
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
ARYWRARTMQEKGNIQGAAELFEKLSVDHPATYYGLMARSQLAKVDPPRLEKVSAEIFAV
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHCHHHEEC
PEAASPWPLFAGPMGEDPHFRAGVELYRLGFKEAVSSEMLAVNRTNLPAESVRLLVLVLH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
EAGDERSAHAVARLALRKDLSGRITPETRVVWEVAYPNAFRDSIEKHTADAGVEPDLLQA
HCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHH
LMREESALDPKALSWAGAMGLTQLMPSTAKGVARELKLKRFSVDQLLQPDLNIRMGAHYL
HHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHCCCCCEEECHHHH
GGLLKRFNGHTPYAVGSYNAGPGAVNRWRSDRPDLALDAWVEEIPISETRGYIKRVLRSF
HHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHC
NTYQLLYGRAPKLPVLKSAAK
CCHHHHHCCCCCCCHHHCCCC
>Mature Secondary Structure
MKPFLSKLAVAASALLMTAQAPAPKAVSSTPAEPEASEAPAQHPSNAPSEAQLSPPPDSE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KAPLPVGYVEVLNPAFPNAAPPTPVVHRGRRYALEDLAPYFGEGKKKEARDAFDRGQYTR
CCCCCCHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHH
ARELLKDEGDSPPVRYLRALAAVRAGDDASAAKEFAALVPDYPALKDRCLTHGGVALESL
HHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
RRFDEAAVLLEQVPPESKLYVDARLALSRVLRKKKDAAGAMAALEPLTSRAAPSWGRNVG
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
AEALMAIADIAAEKKDKAAERAALWRLWAAHPLSALSKQAEKRLKGQTPPVDARVGRGEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVELHRNKAGLEQLEPLLPKLALPDPLACRAHFAFGKGMRKERQHTRAIQVLTPVAEKCQ
HHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
DRDLLARVLYVLGSSRSIVDQARGTETYERLAREFPDHSFADDGLFYAADLYLKTGRPKE
CHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHH
AMARLDTLARLYPQGDFLGEALFKAFWIARTTGVEDSGLSFLDRIEAQFAKADESYDVER
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
ARYWRARTMQEKGNIQGAAELFEKLSVDHPATYYGLMARSQLAKVDPPRLEKVSAEIFAV
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHCHHHEEC
PEAASPWPLFAGPMGEDPHFRAGVELYRLGFKEAVSSEMLAVNRTNLPAESVRLLVLVLH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
EAGDERSAHAVARLALRKDLSGRITPETRVVWEVAYPNAFRDSIEKHTADAGVEPDLLQA
HCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHH
LMREESALDPKALSWAGAMGLTQLMPSTAKGVARELKLKRFSVDQLLQPDLNIRMGAHYL
HHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHCCCCCEEECHHHH
GGLLKRFNGHTPYAVGSYNAGPGAVNRWRSDRPDLALDAWVEEIPISETRGYIKRVLRSF
HHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHC
NTYQLLYGRAPKLPVLKSAAK
CCHHHHHCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]