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The map label for this gene is mrpB [H]

Identifier: 108758595

GI number: 108758595

Start: 6400823

End: 6402289

Strand: Direct

Name: mrpB [H]

Synonym: MXAN_5124

Alternate gene names: 108758595

Gene position: 6400823-6402289 (Clockwise)

Preceding gene: 108760199

Following gene: 108762359

Centisome position: 70.03

GC content: 67.89

Gene sequence:

>1467_bases
ATGGAGACCCTTCTCATCGTCGACGACGACGTCTCGCTCCTCGAAACGCTGAAGATGCATTTCGAGGAGATCGAGCAGGA
CGGCCAGCCCCGCTACCAGGTGGCCACCGCCACCAGCGCCGCGGCAGGACTGCGCGCTGCCCAGGAGGCCATGCCCAGCG
TGGTCATCCTCGACATGATGCTCCCGGACCGCACGGGTCTGGAAATCATCGAGGAGATGAAGGGCCTGTGCGGGGACGCG
CGCATCATCCTGGTCACCGCGTACCACGACATGGAGACCACCATCCGGGCCATGAAGGCCGGGGCTTTCGACTACATCCA
CAAGCCCTTCCCGGACCCGGCCGCCCTGGACCTGGTGGTGGAGCGCGCGCTGGAGTACCGCCAGCTGTCGCGCCGCGCGG
ACGAGGTCCACCGGGAGAACGCCGCCGCCCGCCTGGGCGACATCGTGGGCACCAGCCCGCTGATGCAGCAACTGGTGAAG
GAGATTGGAAAAGTCACCGGCAGCCACGCCACCGTGCTGATTACGGGCGAGAGCGGCACCGGCAAGGAGCTCATCGCCCG
CGTCATCCACAACTACTCCTACGACGAGCCCCGGCCCTTCATCGGCATCAACTGTTCGGCCATCGTCGACACGCTGCTGG
AGAGCGAGCTCTTCGGCCACGAGAAGGGCGCCTTCACCGGCGCCACGGCGGGCAAGCCGGGCAAGTTCGAGCTGGCCGAG
GACGGCACGGTGTTCCTGGACGAGATTGGCGACATGTCGCTGATGCTCCAGGCCAAGCTCCTGCGCGTACTGCAGGAGCG
CGAGTTCGAGCGCGTGGGCGGCGTCAAACGCATCAAGCTGCGCGCCCGCGTCATCGCCGCCACCCACCGCGCGCTGGTGG
AGGAGGTGGAGGCCGGCCGCTTCCGCGAGGACCTCTACCAACGCCTCAAGGTCATCACCCTCCAGATTCCCCCGCTGCGC
GAGCGGCGCGAGGACATCCCCCCGCTGGTGAAGCACCTGCTCGAGCGCATCAACGAGAAGGTCCACAAGCGCGTCACCCG
CGTGCCCGGCGAGGTCATGGAGCGCCTCACCCGCCTGCCCTGGCGCGGCAACGTGCGCGAACTGGAGAACGTGCTCACCC
GCGCCGTGGTGCTGGCCCCCGGTGACGTCCTGCGCGGGGACGATTTGCCCGCCCTGGAGTCTCCCCTCCCCTCGCCGGAG
CCGGGCCGGGCCACCTCCGCCCACCACACCGCCAGCGCCATGTTCGCCGCGCCGGCCGAGGACGACGCCAGCCTGATTCC
CACTTTGGAAGAGGCCGAGCGCCAGCTCATCGCCCGTGCGATGACCGTCACGAAGGGGCACAAGGGTCGCACCTGCCAGA
TCCTTGGAATCAGCCGCCCCACCCTGGAGCGCAAGCTCCAGAAGTACGGTCTTGCACAGAGCCAGAGCCCTCAAGTGCAC
ACCTTCCCTGTGAAGGATGCGTTGTGA

Upstream 100 bases:

>100_bases
CTGCGCCGGGTGATGACGGGCGTGGGGGGAGACGTGGCCGCCCGGGGCAGCGCCGAGCCGGGCGTGGTGCTGACGCTGAC
GTTCGCCACCTGAGAGCGCC

Downstream 100 bases:

>100_bases
GCACTCTTGGACAGTGTCGCTGCTCCGACACTCATGCCCCCGGTTTGAACGTTTCGTTCAAATTGATCAGACTGTTTGAA
CGTTCTGTTTCAGGTTTTGG

Product: sigma-54 dependent DNA-binding response regulator MrpB

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
METLLIVDDDVSLLETLKMHFEEIEQDGQPRYQVATATSAAAGLRAAQEAMPSVVILDMMLPDRTGLEIIEEMKGLCGDA
RIILVTAYHDMETTIRAMKAGAFDYIHKPFPDPAALDLVVERALEYRQLSRRADEVHRENAAARLGDIVGTSPLMQQLVK
EIGKVTGSHATVLITGESGTGKELIARVIHNYSYDEPRPFIGINCSAIVDTLLESELFGHEKGAFTGATAGKPGKFELAE
DGTVFLDEIGDMSLMLQAKLLRVLQEREFERVGGVKRIKLRARVIAATHRALVEEVEAGRFREDLYQRLKVITLQIPPLR
ERREDIPPLVKHLLERINEKVHKRVTRVPGEVMERLTRLPWRGNVRELENVLTRAVVLAPGDVLRGDDLPALESPLPSPE
PGRATSAHHTASAMFAAPAEDDASLIPTLEEAERQLIARAMTVTKGHKGRTCQILGISRPTLERKLQKYGLAQSQSPQVH
TFPVKDAL

Sequences:

>Translated_488_residues
METLLIVDDDVSLLETLKMHFEEIEQDGQPRYQVATATSAAAGLRAAQEAMPSVVILDMMLPDRTGLEIIEEMKGLCGDA
RIILVTAYHDMETTIRAMKAGAFDYIHKPFPDPAALDLVVERALEYRQLSRRADEVHRENAAARLGDIVGTSPLMQQLVK
EIGKVTGSHATVLITGESGTGKELIARVIHNYSYDEPRPFIGINCSAIVDTLLESELFGHEKGAFTGATAGKPGKFELAE
DGTVFLDEIGDMSLMLQAKLLRVLQEREFERVGGVKRIKLRARVIAATHRALVEEVEAGRFREDLYQRLKVITLQIPPLR
ERREDIPPLVKHLLERINEKVHKRVTRVPGEVMERLTRLPWRGNVRELENVLTRAVVLAPGDVLRGDDLPALESPLPSPE
PGRATSAHHTASAMFAAPAEDDASLIPTLEEAERQLIARAMTVTKGHKGRTCQILGISRPTLERKLQKYGLAQSQSPQVH
TFPVKDAL
>Mature_488_residues
METLLIVDDDVSLLETLKMHFEEIEQDGQPRYQVATATSAAAGLRAAQEAMPSVVILDMMLPDRTGLEIIEEMKGLCGDA
RIILVTAYHDMETTIRAMKAGAFDYIHKPFPDPAALDLVVERALEYRQLSRRADEVHRENAAARLGDIVGTSPLMQQLVK
EIGKVTGSHATVLITGESGTGKELIARVIHNYSYDEPRPFIGINCSAIVDTLLESELFGHEKGAFTGATAGKPGKFELAE
DGTVFLDEIGDMSLMLQAKLLRVLQEREFERVGGVKRIKLRARVIAATHRALVEEVEAGRFREDLYQRLKVITLQIPPLR
ERREDIPPLVKHLLERINEKVHKRVTRVPGEVMERLTRLPWRGNVRELENVLTRAVVLAPGDVLRGDDLPALESPLPSPE
PGRATSAHHTASAMFAAPAEDDASLIPTLEEAERQLIARAMTVTKGHKGRTCQILGISRPTLERKLQKYGLAQSQSPQVH
TFPVKDAL

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=470, Percent_Identity=41.2765957446808, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1790437, Length=465, Percent_Identity=41.2903225806452, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1790299, Length=473, Percent_Identity=39.5348837209302, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=37.8378378378378, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1789087, Length=370, Percent_Identity=43.2432432432432, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI87082117, Length=367, Percent_Identity=41.9618528610354, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=42.2492401215805, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082152, Length=267, Percent_Identity=47.940074906367, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786524, Length=342, Percent_Identity=39.1812865497076, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787583, Length=351, Percent_Identity=35.8974358974359, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI87081872, Length=334, Percent_Identity=34.1317365269461, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI87081858, Length=309, Percent_Identity=31.0679611650485, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1789828, Length=245, Percent_Identity=34.6938775510204, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54110; Mature: 54110

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLLIVDDDVSLLETLKMHFEEIEQDGQPRYQVATATSAAAGLRAAQEAMPSVVILDMM
CCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEC
LPDRTGLEIIEEMKGLCGDARIILVTAYHDMETTIRAMKAGAFDYIHKPFPDPAALDLVV
CCCCCHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHH
ERALEYRQLSRRADEVHRENAAARLGDIVGTSPLMQQLVKEIGKVTGSHATVLITGESGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
GKELIARVIHNYSYDEPRPFIGINCSAIVDTLLESELFGHEKGAFTGATAGKPGKFELAE
HHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECC
DGTVFLDEIGDMSLMLQAKLLRVLQEREFERVGGVKRIKLRARVIAATHRALVEEVEAGR
CCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FREDLYQRLKVITLQIPPLRERREDIPPLVKHLLERINEKVHKRVTRVPGEVMERLTRLP
HHHHHHHHHHEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
WRGNVRELENVLTRAVVLAPGDVLRGDDLPALESPLPSPEPGRATSAHHTASAMFAAPAE
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCC
DDASLIPTLEEAERQLIARAMTVTKGHKGRTCQILGISRPTLERKLQKYGLAQSQSPQVH
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCEE
TFPVKDAL
ECCCCCCC
>Mature Secondary Structure
METLLIVDDDVSLLETLKMHFEEIEQDGQPRYQVATATSAAAGLRAAQEAMPSVVILDMM
CCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEC
LPDRTGLEIIEEMKGLCGDARIILVTAYHDMETTIRAMKAGAFDYIHKPFPDPAALDLVV
CCCCCHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHH
ERALEYRQLSRRADEVHRENAAARLGDIVGTSPLMQQLVKEIGKVTGSHATVLITGESGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
GKELIARVIHNYSYDEPRPFIGINCSAIVDTLLESELFGHEKGAFTGATAGKPGKFELAE
HHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECC
DGTVFLDEIGDMSLMLQAKLLRVLQEREFERVGGVKRIKLRARVIAATHRALVEEVEAGR
CCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FREDLYQRLKVITLQIPPLRERREDIPPLVKHLLERINEKVHKRVTRVPGEVMERLTRLP
HHHHHHHHHHEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
WRGNVRELENVLTRAVVLAPGDVLRGDDLPALESPLPSPEPGRATSAHHTASAMFAAPAE
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCC
DDASLIPTLEEAERQLIARAMTVTKGHKGRTCQILGISRPTLERKLQKYGLAQSQSPQVH
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCEE
TFPVKDAL
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]