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The map label for this gene is pbpE [H]

Identifier: 108758564

GI number: 108758564

Start: 2748720

End: 2750129

Strand: Reverse

Name: pbpE [H]

Synonym: MXAN_2364

Alternate gene names: 108758564

Gene position: 2750129-2748720 (Counterclockwise)

Preceding gene: 108757227

Following gene: 108759013

Centisome position: 30.09

GC content: 65.32

Gene sequence:

>1410_bases
GTGGCACCGCTGGCGGAGGCGGCGAACCGGAAGCAGGAGGTAGACCGGTACATCCATGGGTTCCACCAGAAGGGCATGTT
CAACGGAACGGCGCTCGTAGCCACGGAGGGGGGCATCCTCCTCAAGAAGGGCTACGGCTCCGCGAACCTCGAGTGGAAGG
TGCCCAACGCGCCGGACACGAAGTTCCGCATCGGCTCCATCACCAAGTCCTTCACGGCAACCGTCATCCTGCAGCTCGTC
GCCGAGGGAAAGCTCCAGCTCGATGACCCCATCACGAAGCACCTGCCGGACTACCGGGGCGACACCGGTGGGCGCGTCAC
CATCACCCACCTGTTGAACCACACCTCCGGCATCCCCAGCTACACGTCAGCCCCCCATTTCCGGGCCGACTCGGTCAACC
CCTATGGCGTCGCGGAGTTCGTGAAGAAGTCCTGCAGTGGCGACCTGGAGTTCGAGCCCGGCACGAAGTACGCGTACAAC
AACTGCGGCTACTACCTGCTGGGCGCCCTCATCGAGAAGCTCACCGGCCAGACGTATGCGCAGGCCGTGCAGGCGCGCAT
CTTCGGGCCACTGGGCATGAAGGACTCCGGCTATGACGTCACCGCCACGGTGCTGCCCAAGCGCGCCAGCGGCTACACGC
CCAGGCCGGGCGGGTACGTCAACGCGCCCTACATCGACATGGGCCAGCCCTATGCCGCCGGCTCGCTGTACTCGACGGTG
GAGGACCTGTACCGCTGGGACCGGGCCTTCTACGGCGACACGCTGATGCCCGCGGAGCTCAAGCAGAAGATGCTCACCCC
GGGCCTTCAGCACTACGGCTTCGGCTGGGCCATTGCCCCCGTGCAACTGCATGACGGCAAGACGAAGCTTCCAGGTATCT
TCCACAGCGGCGGCATCAACGGCTTCTCATCCCTCCTGGTCCGCCTGCCCGAGCGCAAGGAGGTCGTCATCCTCCTCGAC
AACGCGACCCACGGCGACCTCCAGGAGATTGCGGGCGGCGTGCTGAGCATCCTCCATGGCATCGCCCCCCGGCAGGCCCG
GATGCCCATCGGCACCGTGATGATGGAGTCTCTCGGCAAGGGCTCCACCGCCGAGGCCATCGCCAACTACCGCGCGCTGA
AGAAGACGAAAGAAGCCCAGTACGACTTCAGCGAATCGCAGTTGAACACGGTGGGCTACCACCTCTTGCGCGACGGGCGC
GTGGTGGATGCCATCGAGGTGTTCAAGCTCAACGTGGAGACGTTCCCCAAGGGGGCCAACTGCTACGACAGCCTCGGCGA
GGCCTACGCCGCCCACGGCGACAAGGAACAGGCCATCGCGAACTACCGCAAGTCCCTGGAGCTGGACCCCAAGAACGAGA
ACGCGGTGAAGATGCTCAAGGAGCTCGAGCAGCCCGCCGCGACGCGGTAG

Upstream 100 bases:

>100_bases
AATCACCCGCATCCCCGCACGTACTGCGCGAAATGAAGGGGAGGTCTGAATGAGATGGCTGAGCACGCGCCTCACGGGCG
TACTGGTGGCATGGGTGCTG

Downstream 100 bases:

>100_bases
GCCGCGAGCCTTCCGACGAAAGCACCGTGCCGGGTGCCGCCAGCGTGGGCGGCACCCGGAAGCACGCCAGGGACTACTTC
CCCTTGGCCTGCTCCTTCGT

Product: beta-lactamase

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAPLAEAANRKQEVDRYIHGFHQKGMFNGTALVATEGGILLKKGYGSANLEWKVPNAPDTKFRIGSITKSFTATVILQLV
AEGKLQLDDPITKHLPDYRGDTGGRVTITHLLNHTSGIPSYTSAPHFRADSVNPYGVAEFVKKSCSGDLEFEPGTKYAYN
NCGYYLLGALIEKLTGQTYAQAVQARIFGPLGMKDSGYDVTATVLPKRASGYTPRPGGYVNAPYIDMGQPYAAGSLYSTV
EDLYRWDRAFYGDTLMPAELKQKMLTPGLQHYGFGWAIAPVQLHDGKTKLPGIFHSGGINGFSSLLVRLPERKEVVILLD
NATHGDLQEIAGGVLSILHGIAPRQARMPIGTVMMESLGKGSTAEAIANYRALKKTKEAQYDFSESQLNTVGYHLLRDGR
VVDAIEVFKLNVETFPKGANCYDSLGEAYAAHGDKEQAIANYRKSLELDPKNENAVKMLKELEQPAATR

Sequences:

>Translated_469_residues
MAPLAEAANRKQEVDRYIHGFHQKGMFNGTALVATEGGILLKKGYGSANLEWKVPNAPDTKFRIGSITKSFTATVILQLV
AEGKLQLDDPITKHLPDYRGDTGGRVTITHLLNHTSGIPSYTSAPHFRADSVNPYGVAEFVKKSCSGDLEFEPGTKYAYN
NCGYYLLGALIEKLTGQTYAQAVQARIFGPLGMKDSGYDVTATVLPKRASGYTPRPGGYVNAPYIDMGQPYAAGSLYSTV
EDLYRWDRAFYGDTLMPAELKQKMLTPGLQHYGFGWAIAPVQLHDGKTKLPGIFHSGGINGFSSLLVRLPERKEVVILLD
NATHGDLQEIAGGVLSILHGIAPRQARMPIGTVMMESLGKGSTAEAIANYRALKKTKEAQYDFSESQLNTVGYHLLRDGR
VVDAIEVFKLNVETFPKGANCYDSLGEAYAAHGDKEQAIANYRKSLELDPKNENAVKMLKELEQPAATR
>Mature_468_residues
APLAEAANRKQEVDRYIHGFHQKGMFNGTALVATEGGILLKKGYGSANLEWKVPNAPDTKFRIGSITKSFTATVILQLVA
EGKLQLDDPITKHLPDYRGDTGGRVTITHLLNHTSGIPSYTSAPHFRADSVNPYGVAEFVKKSCSGDLEFEPGTKYAYNN
CGYYLLGALIEKLTGQTYAQAVQARIFGPLGMKDSGYDVTATVLPKRASGYTPRPGGYVNAPYIDMGQPYAAGSLYSTVE
DLYRWDRAFYGDTLMPAELKQKMLTPGLQHYGFGWAIAPVQLHDGKTKLPGIFHSGGINGFSSLLVRLPERKEVVILLDN
ATHGDLQEIAGGVLSILHGIAPRQARMPIGTVMMESLGKGSTAEAIANYRALKKTKEAQYDFSESQLNTVGYHLLRDGRV
VDAIEVFKLNVETFPKGANCYDSLGEAYAAHGDKEQAIANYRKSLELDPKNENAVKMLKELEQPAATR

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI26051231, Length=247, Percent_Identity=28.7449392712551, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1790593, Length=348, Percent_Identity=24.1379310344828, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786574, Length=339, Percent_Identity=28.023598820059, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082101, Length=224, Percent_Identity=29.9107142857143, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI115533026, Length=347, Percent_Identity=27.6657060518732, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17550934, Length=173, Percent_Identity=29.4797687861272, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 51245; Mature: 51114

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS50005 TPR L=RR ; PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPLAEAANRKQEVDRYIHGFHQKGMFNGTALVATEGGILLKKGYGSANLEWKVPNAPDT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCEEEECCCCCCC
KFRIGSITKSFTATVILQLVAEGKLQLDDPITKHLPDYRGDTGGRVTITHLLNHTSGIPS
CEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEHHHHHHHCCCCCC
YTSAPHFRADSVNPYGVAEFVKKSCSGDLEFEPGTKYAYNNCGYYLLGALIEKLTGQTYA
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCEECCCCHHHHHHHHHHHCCHHHH
QAVQARIFGPLGMKDSGYDVTATVLPKRASGYTPRPGGYVNAPYIDMGQPYAAGSLYSTV
HHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHH
EDLYRWDRAFYGDTLMPAELKQKMLTPGLQHYGFGWAIAPVQLHDGKTKLPGIFHSGGIN
HHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCCEEEEEEEECCCCCCCCCEEECCCCC
GFSSLLVRLPERKEVVILLDNATHGDLQEIAGGVLSILHGIAPRQARMPIGTVMMESLGK
HHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHCCC
GSTAEAIANYRALKKTKEAQYDFSESQLNTVGYHLLRDGRVVDAIEVFKLNVETFPKGAN
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEHEEECHHHCCCCCC
CYDSLGEAYAAHGDKEQAIANYRKSLELDPKNENAVKMLKELEQPAATR
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
APLAEAANRKQEVDRYIHGFHQKGMFNGTALVATEGGILLKKGYGSANLEWKVPNAPDT
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCEEEECCCCCCC
KFRIGSITKSFTATVILQLVAEGKLQLDDPITKHLPDYRGDTGGRVTITHLLNHTSGIPS
CEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEHHHHHHHCCCCCC
YTSAPHFRADSVNPYGVAEFVKKSCSGDLEFEPGTKYAYNNCGYYLLGALIEKLTGQTYA
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCEECCCCHHHHHHHHHHHCCHHHH
QAVQARIFGPLGMKDSGYDVTATVLPKRASGYTPRPGGYVNAPYIDMGQPYAAGSLYSTV
HHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHH
EDLYRWDRAFYGDTLMPAELKQKMLTPGLQHYGFGWAIAPVQLHDGKTKLPGIFHSGGIN
HHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCCEEEEEEEECCCCCCCCCEEECCCCC
GFSSLLVRLPERKEVVILLDNATHGDLQEIAGGVLSILHGIAPRQARMPIGTVMMESLGK
HHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHCCC
GSTAEAIANYRALKKTKEAQYDFSESQLNTVGYHLLRDGRVVDAIEVFKLNVETFPKGAN
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEHEEECHHHCCCCCC
CYDSLGEAYAAHGDKEQAIANYRKSLELDPKNENAVKMLKELEQPAATR
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]