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The map label for this gene is nfi

Identifier: 108758366

GI number: 108758366

Start: 1764109

End: 1764816

Strand: Direct

Name: nfi

Synonym: MXAN_1503

Alternate gene names: 108758366

Gene position: 1764109-1764816 (Clockwise)

Preceding gene: 108763949

Following gene: 108757603

Centisome position: 19.3

GC content: 69.63

Gene sequence:

>708_bases
GTGTGCATGGCGCTTCAGCCCGAAGTGAGCAGGTGGGACGTCACGCCTTCGGAGGCGGTGGAGCTGCAGCGCCGGCTGCG
TGAGCAGTTGGTGCTCCGGCCGCCCCCTGGGCTGAAGGTGGAGCGCATCGCGGGCGCGGACATCTCCACGGAGAAGGGGA
AGGACACCGGCTTTGGTGGGTTCGTCGTCCTGGACGTGGAGACGTTGGCGCCGGTGGCCCAGTCTGAGGCCGTGGTGACG
TTGCACTTTCCCTACGTGCCGGGCCTGCTGTCCTTCCGCGAGCTGCCCACCATCGCCGCTGCCTGGGAACGGCTCACGGT
GCGCCCCGATGTGGTCATCTTCGATGGACAAGGCACCGCCCATCCCCGGCGCATGGGGATTGCGTGTCACGGAGGGTTGC
TGTTCGGCGTGCCATCCATCGGCTGTGCCAAGTCCCTGCTGGTGGGGACGCACGGACCGCTGGGCGAGGCGAGAGGCTCC
ACCGCGCCCCTCATGCACCGGGGCGAGGTCGTGGGCATGGCGGTGCGCACGCGCAAGGGCGTGCAGCCCGTCTACGTGTC
CCCGGGCCACCTGATGGACCTGCCCACGGCGGTGGAGTGGGTGCTCAAGGTGAGCCCGAAGTACCGCGAGCCGGAGACCA
CGCGGCATGCGCACCGGCTGGTCAACGCACTCCGTCGCGCGGACGGAGAGGCCGCCGAACTGGAGTAG

Upstream 100 bases:

>100_bases
GCGGGTTCCAAAGGGGAACGGCCAGTTCACCAGTCCACGCTGAAGGGACGCCATGGGTTGCCCTTGAGGTCAGAGCCTTC
AGATTGGCAGTGGAGGTTGC

Downstream 100 bases:

>100_bases
GTGCCGGCCCCTGATTTTGACCGCCGTCCCAACCGACTCTATGTCCGGAGGTGGCTCCATCGCGTGGGGCCTCAAGGAAC
GGCGGCTCGGGCGTCTGTCT

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MCMALQPEVSRWDVTPSEAVELQRRLREQLVLRPPPGLKVERIAGADISTEKGKDTGFGGFVVLDVETLAPVAQSEAVVT
LHFPYVPGLLSFRELPTIAAAWERLTVRPDVVIFDGQGTAHPRRMGIACHGGLLFGVPSIGCAKSLLVGTHGPLGEARGS
TAPLMHRGEVVGMAVRTRKGVQPVYVSPGHLMDLPTAVEWVLKVSPKYREPETTRHAHRLVNALRRADGEAAELE

Sequences:

>Translated_235_residues
MCMALQPEVSRWDVTPSEAVELQRRLREQLVLRPPPGLKVERIAGADISTEKGKDTGFGGFVVLDVETLAPVAQSEAVVT
LHFPYVPGLLSFRELPTIAAAWERLTVRPDVVIFDGQGTAHPRRMGIACHGGLLFGVPSIGCAKSLLVGTHGPLGEARGS
TAPLMHRGEVVGMAVRTRKGVQPVYVSPGHLMDLPTAVEWVLKVSPKYREPETTRHAHRLVNALRRADGEAAELE
>Mature_235_residues
MCMALQPEVSRWDVTPSEAVELQRRLREQLVLRPPPGLKVERIAGADISTEKGKDTGFGGFVVLDVETLAPVAQSEAVVT
LHFPYVPGLLSFRELPTIAAAWERLTVRPDVVIFDGQGTAHPRRMGIACHGGLLFGVPSIGCAKSLLVGTHGPLGEARGS
TAPLMHRGEVVGMAVRTRKGVQPVYVSPGHLMDLPTAVEWVLKVSPKYREPETTRHAHRLVNALRRADGEAAELE

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=203, Percent_Identity=34.4827586206897, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI257467548, Length=120, Percent_Identity=36.6666666666667, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI257467550, Length=120, Percent_Identity=36.6666666666667, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI87082357, Length=192, Percent_Identity=41.1458333333333, Blast_Score=153, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_MYXXD (Q1DC68)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_629755.1
- ProteinModelPortal:   Q1DC68
- STRING:   Q1DC68
- GeneID:   4104549
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_1503
- TIGR:   MXAN_1503
- eggNOG:   COG1515
- HOGENOM:   HBG716360
- OMA:   SVGHRIG
- PhylomeDB:   Q1DC68
- BioCyc:   MXAN246197:MXAN_1503-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 25484; Mature: 25484

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCMALQPEVSRWDVTPSEAVELQRRLREQLVLRPPPGLKVERIAGADISTEKGKDTGFGG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCCCCCCCCCC
FVVLDVETLAPVAQSEAVVTLHFPYVPGLLSFRELPTIAAAWERLTVRPDVVIFDGQGTA
EEEEEHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCHHHHHHHHEECCCCEEEECCCCCC
HPRRMGIACHGGLLFGVPSIGCAKSLLVGTHGPLGEARGSTAPLMHRGEVVGMAVRTRKG
CHHHEEEEECCCEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEEEEHHCCC
VQPVYVSPGHLMDLPTAVEWVLKVSPKYREPETTRHAHRLVNALRRADGEAAELE
CCCEEECCCCEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MCMALQPEVSRWDVTPSEAVELQRRLREQLVLRPPPGLKVERIAGADISTEKGKDTGFGG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCCCCCCCCCC
FVVLDVETLAPVAQSEAVVTLHFPYVPGLLSFRELPTIAAAWERLTVRPDVVIFDGQGTA
EEEEEHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCHHHHHHHHEECCCCEEEECCCCCC
HPRRMGIACHGGLLFGVPSIGCAKSLLVGTHGPLGEARGSTAPLMHRGEVVGMAVRTRKG
CHHHEEEEECCCEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEEEEHHCCC
VQPVYVSPGHLMDLPTAVEWVLKVSPKYREPETTRHAHRLVNALRRADGEAAELE
CCCEEECCCCEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA