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The map label for this gene is truB [H]

Identifier: 108758349

GI number: 108758349

Start: 2403047

End: 2403916

Strand: Direct

Name: truB [H]

Synonym: MXAN_2071

Alternate gene names: 108758349

Gene position: 2403047-2403916 (Clockwise)

Preceding gene: 108757332

Following gene: 108762422

Centisome position: 26.29

GC content: 70.8

Gene sequence:

>870_bases
ATGGACGGCGTCCTCGTCATTGACAAGCCCACCGGTCCCACGTCGTTCGACGTGGTCCGCCAGGTGCGTTCGCTGCTTCG
CCTGAAGAAGGTGGGCCACACGGGGACGCTGGACCCGCTGGCCACTGGAGTGCTGCCGCTCTGCCTGGGGGAAGCCACCA
AGGTCGCGGGCTTCATCACCGAGGGCGACAAGGCCTACGACGCCACGGTGCGTCTGGGCTCGGAGACGGACACCCTGGAC
GCGGAAGGGCAGGTGACGGCGCAGGCGCCCGTGCCCGCCCTGACGCCCGCGCTGATTGAATCCGCGTTGGCGCGCTTCCG
GGGCACTTTCGACCAGGTCCCACCCATGTATTCGGCCGTCAAGGTGGGCGGGAAGCGGCTCTACGAACTGGCTCGTGCGG
GCGAGGAAGTGGAGCGCGCCGCCCGTCAGGTGACGGTGTACGAGTTGGTGCTGCGGGACTTCTCCGCCGAGCGCCTGCAG
CTGTCGGTGCGCTGCTCCAAGGGCTTCTTCGTGCGCACCCTGGCCCAGGAGGTGGGCCGGGCGCTGGGTTGTGGTGCCCA
CCTGGAGGCACTGCGGCGCACCGCCAGTGGCCCCTTCACCCTGGCGCAGGCGCTGCCGCTGGCGGACGTGCCGGAGCTGC
TGAAGGAGGGCGCGCTGGCCAGCCGGCTGATGACCATGTCGCATGCGCTGGTGGAACTGCCGGAGGTCCGGGTGGGCGCC
GCCGATGCCAAGCGCGTCTCCCACGGTGTCCCGGTGGAGGTCCCCGCCGGGAAGGTGGGACGGGTGCGCGTCATGGGCCC
GGACGACGCGCTGCTGGCGGTGGCCGAAGTGGCCGGTGGCCGCCTGCGCTATTTGCGCGTCCTGGTGTAA

Upstream 100 bases:

>100_bases
AATCGGTGGGCGAAGGTGACAAGATTGACCGGCTGCTGCGCGAAGTCCGCAACAAGGAAGGCTGGTAGGTCCAGCTCCGG
GCCTCCACGATTGGCGTGCC

Downstream 100 bases:

>100_bases
CGCCGGGATTTTTCGCGGGGTTGTGGCTGTACGTCTTCTCGTGGTCCCGGACTTTCCGGGGCTGGCGGGCAGGCGTCGAA
GGTTGACCCCACGGGGGGTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDGVLVIDKPTGPTSFDVVRQVRSLLRLKKVGHTGTLDPLATGVLPLCLGEATKVAGFITEGDKAYDATVRLGSETDTLD
AEGQVTAQAPVPALTPALIESALARFRGTFDQVPPMYSAVKVGGKRLYELARAGEEVERAARQVTVYELVLRDFSAERLQ
LSVRCSKGFFVRTLAQEVGRALGCGAHLEALRRTASGPFTLAQALPLADVPELLKEGALASRLMTMSHALVELPEVRVGA
ADAKRVSHGVPVEVPAGKVGRVRVMGPDDALLAVAEVAGGRLRYLRVLV

Sequences:

>Translated_289_residues
MDGVLVIDKPTGPTSFDVVRQVRSLLRLKKVGHTGTLDPLATGVLPLCLGEATKVAGFITEGDKAYDATVRLGSETDTLD
AEGQVTAQAPVPALTPALIESALARFRGTFDQVPPMYSAVKVGGKRLYELARAGEEVERAARQVTVYELVLRDFSAERLQ
LSVRCSKGFFVRTLAQEVGRALGCGAHLEALRRTASGPFTLAQALPLADVPELLKEGALASRLMTMSHALVELPEVRVGA
ADAKRVSHGVPVEVPAGKVGRVRVMGPDDALLAVAEVAGGRLRYLRVLV
>Mature_289_residues
MDGVLVIDKPTGPTSFDVVRQVRSLLRLKKVGHTGTLDPLATGVLPLCLGEATKVAGFITEGDKAYDATVRLGSETDTLD
AEGQVTAQAPVPALTPALIESALARFRGTFDQVPPMYSAVKVGGKRLYELARAGEEVERAARQVTVYELVLRDFSAERLQ
LSVRCSKGFFVRTLAQEVGRALGCGAHLEALRRTASGPFTLAQALPLADVPELLKEGALASRLMTMSHALVELPEVRVGA
ADAKRVSHGVPVEVPAGKVGRVRVMGPDDALLAVAEVAGGRLRYLRVLV

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=224, Percent_Identity=40.625, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI4503337, Length=197, Percent_Identity=31.4720812182741, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI215599015, Length=197, Percent_Identity=31.4720812182741, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI2367200, Length=288, Percent_Identity=38.8888888888889, Blast_Score=205, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17553978, Length=197, Percent_Identity=29.9492385786802, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=201, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324037, Length=133, Percent_Identity=39.0977443609023, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364189, Length=197, Percent_Identity=30.4568527918782, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281364187, Length=197, Percent_Identity=30.4568527918782, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281364185, Length=197, Percent_Identity=30.4568527918782, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281364183, Length=197, Percent_Identity=30.4568527918782, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI62471759, Length=197, Percent_Identity=30.4568527918782, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17975520, Length=197, Percent_Identity=30.4568527918782, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 30727; Mature: 30727

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGVLVIDKPTGPTSFDVVRQVRSLLRLKKVGHTGTLDPLATGVLPLCLGEATKVAGFIT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHEEE
EGDKAYDATVRLGSETDTLDAEGQVTAQAPVPALTPALIESALARFRGTFDQVPPMYSAV
CCCCCEEEEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCHHCCCHHHHHH
KVGGKRLYELARAGEEVERAARQVTVYELVLRDFSAERLQLSVRCSKGFFVRTLAQEVGR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
ALGCGAHLEALRRTASGPFTLAQALPLADVPELLKEGALASRLMTMSHALVELPEVRVGA
HHCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCC
ADAKRVSHGVPVEVPAGKVGRVRVMGPDDALLAVAEVAGGRLRYLRVLV
HHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHEEEC
>Mature Secondary Structure
MDGVLVIDKPTGPTSFDVVRQVRSLLRLKKVGHTGTLDPLATGVLPLCLGEATKVAGFIT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHEEE
EGDKAYDATVRLGSETDTLDAEGQVTAQAPVPALTPALIESALARFRGTFDQVPPMYSAV
CCCCCEEEEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCHHCCCHHHHHH
KVGGKRLYELARAGEEVERAARQVTVYELVLRDFSAERLQLSVRCSKGFFVRTLAQEVGR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
ALGCGAHLEALRRTASGPFTLAQALPLADVPELLKEGALASRLMTMSHALVELPEVRVGA
HHCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCC
ADAKRVSHGVPVEVPAGKVGRVRVMGPDDALLAVAEVAGGRLRYLRVLV
HHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA